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Articles 1201 - 1230 of 3635

Full-Text Articles in Other Environmental Sciences

Increasing Atmospheric Co2 Concentrations Outweighs Effects Of Stand Density In Determining Growth And Water Use Efficiency In Pinus Ponderosa Of The Semi-Arid Grasslands Of Nebraska (U.S.A.), Giovanna Battipaglia, Tala Awada, Rahel Aus Der Au, Michele Innangi, Matthias Saurer, Paolo Cherubini Jan 2020

Increasing Atmospheric Co2 Concentrations Outweighs Effects Of Stand Density In Determining Growth And Water Use Efficiency In Pinus Ponderosa Of The Semi-Arid Grasslands Of Nebraska (U.S.A.), Giovanna Battipaglia, Tala Awada, Rahel Aus Der Au, Michele Innangi, Matthias Saurer, Paolo Cherubini

School of Natural Resources: Faculty Publications

This study investigated the impacts of environmental (e.g., climate and CO2 level) and ecological (e.g., stand density) factors on the long-term growth and physiology of pon- derosa pine (Pinus ponderosa) in a semi-arid north American grassland. We hypothesized that ponderosa pine long-term growth patterns were positively influenced by an increase in atmospheric CO2 concentrations and a decrease in stand density. To test this hypothesis, comparison of long-term trends in tree-ring width and carbon and oxygen stable isotopic composition of trees growing in dense and sparse forest stands were carried out at two sites located in the Nebraska National Forest. Results …


Linking Drought Impacts To Drought Severity At The State Level, M. Noel, K. Smith, D. Bathke, B. Fuchs, D. Gutzmer, T. Haigh, M. Hayes, M. Podebradska, C. Shield, M. Svoboda Jan 2020

Linking Drought Impacts To Drought Severity At The State Level, M. Noel, K. Smith, D. Bathke, B. Fuchs, D. Gutzmer, T. Haigh, M. Hayes, M. Podebradska, C. Shield, M. Svoboda

School of Natural Resources: Faculty Publications

No abstract provided.


Diurnal Activity Patterns Of Walia Ibex (Capra Walie) In Simien Mountains National Park, Ethiopia, D. Ejigu, A. Bekele, L.A. Powell Jan 2020

Diurnal Activity Patterns Of Walia Ibex (Capra Walie) In Simien Mountains National Park, Ethiopia, D. Ejigu, A. Bekele, L.A. Powell

School of Natural Resources: Faculty Publications

No abstract provided.


Biotic And Abiotic Factors Influencing Arsenic Biogeochemistry And Toxicity In Fluvial Ecosystems: A Review, Laura Barral-Fraga, María Teresa Barral, Keeley L. Macneill, Diego Martiñá-Prieto, Soizic Morin, María Carolina Rodríguez-Castro, Baigal-Amar Tuulaikhuu, Helena Guasch Jan 2020

Biotic And Abiotic Factors Influencing Arsenic Biogeochemistry And Toxicity In Fluvial Ecosystems: A Review, Laura Barral-Fraga, María Teresa Barral, Keeley L. Macneill, Diego Martiñá-Prieto, Soizic Morin, María Carolina Rodríguez-Castro, Baigal-Amar Tuulaikhuu, Helena Guasch

School of Natural Resources: Faculty Publications

This review is focused on the biogeochemistry of arsenic in freshwaters and, especially, on the key role that benthic microalgae and prokaryotic communities from biofilms play together in through speciation, distribution, and cycling. These microorganisms incorporate the dominant iAs (inorganic arsenic) form and may transform it to other arsenic forms through metabolic or detoxifying processes. These transformations have a big impact on the environmental behavior of arsenic because different chemical forms exhibit differences in mobility and toxicity. Moreover, exposure to toxicants may alter the physiology and structure of biofilms, leading to changes in ecosystem function and trophic relations. In this …


The Pocatello Supply Depot: A History And Update, Patrick A. Darrow Jan 2020

The Pocatello Supply Depot: A History And Update, Patrick A. Darrow

Vertebrate Pest Conference Proceedings: 29th (2020)

Since the Pocatello Supply Depot (PSD) was created in 1936, the PSD has gone through many transformation and organizational changes. The most recent of these changes occurred in 2014, when the PSD transitioned from a cooperative service agreement between the U.S. Department of Agriculture (USDA) and the Greater Pocatello Chamber of Commerce, to a fully federal facility within USDA. Despite the many organizational changes, the purpose of the PSD has remained the same. The PSD manufactures and provides specialized wildlife damage management materials and services that are not readily available from commercial sources, for use by USDA Wildlife Services (WS), …


Assessing Beaver Occupancy And Dam Building Potential: A Case Study In The Umpqua Watershed Of Southwestern Oregon, Vanessa M. Petro, John Stevenson, Jimmy Taylor Jan 2020

Assessing Beaver Occupancy And Dam Building Potential: A Case Study In The Umpqua Watershed Of Southwestern Oregon, Vanessa M. Petro, John Stevenson, Jimmy Taylor

Vertebrate Pest Conference Proceedings: 29th (2020)

Interest in beaver-related restoration is growing in the western U.S. but understanding the basic ecology of American beaver and their population dynamics is often overlooked when integrating beaver into stream restoration goals. Our study investigated the spatial-temporal distribution of beaver colonies and their damming activities to better inform stream restoration projects in the West Fork Cow Creek Basin of the Umpqua Watershed in southwestern Oregon. During fall 2017, we conducted beaver activity surveys at 144 randomly selected reaches predicted to be either suitable or unsuitable for damming, but suitable for beaver occupation. We categorized beaver use at each reach using …


Automated Aerial Baiting For Invasive Brown Treesnake Control: System Overview And Program Status, Shane R. Siers, John D. Eisemann, William C. Pitt, Larry Clark, Scott M. Goetz, Robert J. Gosnell, Aaron F. Collins, Eric T. Hileman, Melia G. Nafus, Amy A. Yackel Adams, Michael C. Messaros, William G.N. Coon Jan 2020

Automated Aerial Baiting For Invasive Brown Treesnake Control: System Overview And Program Status, Shane R. Siers, John D. Eisemann, William C. Pitt, Larry Clark, Scott M. Goetz, Robert J. Gosnell, Aaron F. Collins, Eric T. Hileman, Melia G. Nafus, Amy A. Yackel Adams, Michael C. Messaros, William G.N. Coon

Vertebrate Pest Conference Proceedings: 29th (2020)

The economically and ecologically catastrophic introduction of invasive brown treesnakes to the Pacific Island of Guam has long served as a cautionary tale about the dangers of invasive species and the seeming impossibility of their management on a landscape scale. USDA Wildlife Services and federal and private partners have engineered a system for the automated manufacture and aerial delivery of toxic baits for landscape-scale suppression of brown treesnakes in large and remote forest plots. The helicopter-borne dispensing module can launch four bait cartridges per second, and a single payload of 3,600 cartridges can treat 30 ha of forest at 120 …


A Preliminary Field Evaluation Of Candidate Repellents To Reduce Black Bear Damage To Western Larch Trees, G. W. Witmer, Michael Pipas Jan 2020

A Preliminary Field Evaluation Of Candidate Repellents To Reduce Black Bear Damage To Western Larch Trees, G. W. Witmer, Michael Pipas

Vertebrate Pest Conference Proceedings: 29th (2020)

Bear damage to western larch trees on intensively managed public and private forest lands of the Intermountain West continues to be a problem for forest managers. Bark stripping and subsequent cambium feeding by bears commences upon den emergence in the spring, when foraging options are most restrictive. Various damage reduction methods are often controversial (snaring, hunting, supplemental feeding), or do not always adequately resolve the problem (silvicultural strategies); hence, a need exists for the development of alternative nonlethal techniques to reduce damage. We tested the efficacy of three candidate repellents (Hot Sauce®, Tree Guard™, and grizzly bear feces) to reduce …


Understanding And Preventing Bird Damage On Dairies, Amber Adams-Progar, Karen Steensma, Stephanie A. Shwiff, Julie L. Elser, Susan Kerr, Tyler P. Caskin Jan 2020

Understanding And Preventing Bird Damage On Dairies, Amber Adams-Progar, Karen Steensma, Stephanie A. Shwiff, Julie L. Elser, Susan Kerr, Tyler P. Caskin

Vertebrate Pest Conference Proceedings: 29th (2020)

Wild birds cause significant damage to dairy farms through the consumption and spoilage of cattle feed. A survey of Washington State dairy farmers revealed approximately $14 million in bird damage losses for the Washington State dairy industry, annually. Furthermore, farms that reported the presence of more than 10,000 birds per day were more likely to report the presence of Salmonella spp. or Johne’s disease (caused by Mycobacterium avium subsp. paratuberculosis). Over the course of three years, we assessed the impact of bird populations on the presence of bacteria in bird feces and the nutritional composition of cattle feed. Five dairies …


Island Rodent Eradications: Little Things Make Big Things Happen, Araceli Samaniego-Herrera, Peter J. Kappes, Shane R. Siers Jan 2020

Island Rodent Eradications: Little Things Make Big Things Happen, Araceli Samaniego-Herrera, Peter J. Kappes, Shane R. Siers

Vertebrate Pest Conference Proceedings: 29th (2020)

Island rodent eradication is often a prerequisite for ecological restoration. These operations have been scaling up in size and complexity, and typically revolve around the thorough distribution of rodenticides in bait stations, by hand broadcast, by helicopter-borne spreading buckets, or by combinations of these methods. Many of the requirements of an eradication can be met by simply purchasing the right materials and following published best practices. However, intangible factors such as training and mindset of personnel are equally critical, and less commonly understood. We briefly review these factors and highlight issues such as the limited pool of experienced eradication practitioners, …


If At First You Don’T Eradicate: Remediating Rat Eradication Failure On Wake Atoll, Peter J. Kappes, Shane R. Siers, Kristen Rex, Chad Hanson Jan 2020

If At First You Don’T Eradicate: Remediating Rat Eradication Failure On Wake Atoll, Peter J. Kappes, Shane R. Siers, Kristen Rex, Chad Hanson

Vertebrate Pest Conference Proceedings: 29th (2020)

Island rodent eradication operations have been remarkably successful at eliminating damages caused by these harmful vertebrate pests. As efforts increase in scale and complexity, so does risk of eradication failure. In this paper we present the example of a partially successful rodent eradication project to highlight how best practices and lessons learned are being integrated to reduce risk of failure during a second attempt. In 2012 the U.S. Air Force (USAF) commissioned an attempted eradication of two rat species from Wake Atoll in the Western Pacific. Asian house rats were successfully eradicated, but it was soon confirmed that some Polynesian …


Laboratory Evaluation Of The Effectiveness Of The Fertility Control Bait Contrapest® On Wild-Captured Black Rats (Rattus Rattus), Shane Siers, Robert T. Sugihara, Israel Leinbach, Brandy R. Pyzyna, Gary Witmer Jan 2020

Laboratory Evaluation Of The Effectiveness Of The Fertility Control Bait Contrapest® On Wild-Captured Black Rats (Rattus Rattus), Shane Siers, Robert T. Sugihara, Israel Leinbach, Brandy R. Pyzyna, Gary Witmer

Vertebrate Pest Conference Proceedings: 29th (2020)

A non-toxic liquid fertility control bait for rats has recently become commercially available (ContraPest® from SenesTech, Inc.). This product contains two chemicals, both of which impair spermatogenesis in male and reduce ovulations in female rats. We tested the efficacy of this bait in wild-caught adult black rats from the island of Hawai’i in a short-term laboratory trial. A control group (n = 25) was offered placebo bait and the treatment group (n = 25) was offered fertility control bait, both ad libitum, during a 15-day introduction period and during the first of four breeding rounds, for a total of 58 …


Development And Testing Of A Matrix For Mongoose Toxic Bait: Nontoxic Bait Acceptance Cage Trials, Shane Siers, Robert T. Sugihara, Israel Leinbach, Daniel Sedgwick, Chris N. Niebuhr, Emily W. Ruell Jan 2020

Development And Testing Of A Matrix For Mongoose Toxic Bait: Nontoxic Bait Acceptance Cage Trials, Shane Siers, Robert T. Sugihara, Israel Leinbach, Daniel Sedgwick, Chris N. Niebuhr, Emily W. Ruell

Vertebrate Pest Conference Proceedings: 29th (2020)

The only pesticide currently registered for mongoose control is a product developed for rats that consists of a hard-cereal bait block. Although the active ingredient (diphacinone) is known to be highly effective for mongoose, previous studies indicate that carnivorous and omnivorous mongooses do not readily consume the hard bait matrix designed for gnawing rodents. A palatable bait matrix with a consistency more appropriate to mongoose dentition and feeding behavior will be required to develop a more effective mongoose pesticide. We evaluated the acceptance and consumption of nontoxic versions of four candidate bait matrices: FOXECUTE® and FOXSHIELD® (Animal Control Technologies, Australia; …


Feasibility Of A Successful Rat Eradication On Wake Atoll Following Initial Partial Failure: Potential Causes, Remedial Actions, And Remaining Knowledge Gaps, Chad Hanson, Kristen Rex, Peter J. Kappes, Shane R. Siers Jan 2020

Feasibility Of A Successful Rat Eradication On Wake Atoll Following Initial Partial Failure: Potential Causes, Remedial Actions, And Remaining Knowledge Gaps, Chad Hanson, Kristen Rex, Peter J. Kappes, Shane R. Siers

Vertebrate Pest Conference Proceedings: 29th (2020)

A 2012 attempt to remove two rat species (Rattus tanezumi and R. exulans) from Wake Atoll was partially successful. R. tanezumi was eradicated from all three islands (Wake, Wilkes, and Peale), and R. exulans was eradicated from Peale. However, R. exulans remained on Wake and Wilkes and have since recovered to very high densities. In 2013, a panel of experts reviewed the eradication operation and offered a list of possible causes of the partial failure. Since that time, further research has been conducted to address several of the issues identified in the review. In this paper, we conduct a current …


Placebo Bait Uptake Trial To Test Feasibility Of Polynesian Rat (Rattus Exulans) Eradication On Wake Atoll, Chris N. Niebuhr, Israel Leinbach, Thomas W. Mcauliffe, Dean K. Foster, Shane R. Siers Jan 2020

Placebo Bait Uptake Trial To Test Feasibility Of Polynesian Rat (Rattus Exulans) Eradication On Wake Atoll, Chris N. Niebuhr, Israel Leinbach, Thomas W. Mcauliffe, Dean K. Foster, Shane R. Siers

Vertebrate Pest Conference Proceedings: 29th (2020)

Rodent eradications have contributed to the recovery of many threatened species, but challenges often exist for campaigns that occur on tropical islands when compared to more temperate regions. A post-operational review of a rat eradication operation on Wake Atoll indicated that certain areas, such as those with high alternative food abundance, may have contributed to the failure to remove all Polynesian rats. We conducted a nontoxic bait uptake trial to evaluate whether the maximum prescribed bait application rate for Brodifacoum-25W rodenticide pellets was sufficient to expose all rats to a lethal dose at three sites on Wake Atoll, including around …


Flash Drought Development And Cascading Impacts Associated With The 2010 Russian Heatwave, J. Christian, J. B. Basara, E. D. Hunt, J. A. Otkin, X. Xiao Jan 2020

Flash Drought Development And Cascading Impacts Associated With The 2010 Russian Heatwave, J. Christian, J. B. Basara, E. D. Hunt, J. A. Otkin, X. Xiao

School of Natural Resources: Faculty Publications

No abstract provided.


The Prairie Post Quarterly Newsletter Of The High Plains Regional Climate Center- January 2020, Natalie Umphlett, Rezaul Mamood, Paul Flanagan, Logan Winters, Kierstin Blomberg, Emilee Lachenmeier, Crystal J. Stiles Jan 2020

The Prairie Post Quarterly Newsletter Of The High Plains Regional Climate Center- January 2020, Natalie Umphlett, Rezaul Mamood, Paul Flanagan, Logan Winters, Kierstin Blomberg, Emilee Lachenmeier, Crystal J. Stiles

Prairie Post: Quarterly Newsletter of the High Plains Regional Climate Center

Inside this issue:

Message from the director........................................1

Staff spotlight...........................1

Year in review............................2

Updates on engagement and tools.....................................3

Observer awards......................3

Overview of regional climate conditions..................................4

AMS annual meeting.............5

Recent and upcoming travel and activities.............................6


Uptake, Metabolism, And Elimination Of Fungicides From Coated Wheat Seeds In Japanese Quail (Coturnix Japonica), Michael S. Gross, Thomas G. Bean, Michelle L. Hladik, Barnett A. Rattner, Kathryn M. Kuivila Jan 2020

Uptake, Metabolism, And Elimination Of Fungicides From Coated Wheat Seeds In Japanese Quail (Coturnix Japonica), Michael S. Gross, Thomas G. Bean, Michelle L. Hladik, Barnett A. Rattner, Kathryn M. Kuivila

United States Geological Survey: Staff Publications

Pesticides coated to the seed surface potentially pose an ecological risk to granivorous birds that consume incompletely buried or spilled seeds. To assess the toxicokinetics of seeds treated with current-use fungicides, Japanese quail ( Coturnix japonica) were orally dosed with commercially coated wheat seeds. Quail were exposed to metalaxyl, tebuconazole, and fludioxonil at either a low dose (0.0655, 0.0308, and 0.0328 mg/kg of body weight, respectively) or a high dose (0.196, 0.0925, and 0.0985 mg/kg of body weight, respectively). Fungicides were rapidly absorbed and distributed to tissues. Tebuconazole was metabolized into tert-butylhydroxy-tebuconazole. All compounds were eliminated to below detection limits …


Winter Use Of A Rock Crevice By Northern Long-Eared Myotis (Myotis Septentrionalis) In Nebraska, Jeremy A. White, Patricia Freeman, Hans W. Otto, Cliff Lemen Jan 2020

Winter Use Of A Rock Crevice By Northern Long-Eared Myotis (Myotis Septentrionalis) In Nebraska, Jeremy A. White, Patricia Freeman, Hans W. Otto, Cliff Lemen

School of Natural Resources: Faculty Publications

We used a combination of acoustic detectors, thermal imaging cameras, and mist netting to confirm the use of a rock crevice by northern long-eared myotis (Myotis septentrionalis) in winter in northeastern Nebraska. Bats roosted in a large crevice in a rock face along the Missouri River, and 5 individuals were captured emerging from the opening of the crevice in March 2017. Our study is the first to confirm the use of a rock-crevice hibernaculum by M. septentrionalis and adds to the growing literature on the winter use of rock crevices by hibernating bats in North America. As white-nose …


Agri-Combs: Open-Path Dual-Comb Spectroscopy Of Livestock Emissions, D. I. Herman, L. C. Hutcherson, C. Weerasekara Et Al. Jan 2020

Agri-Combs: Open-Path Dual-Comb Spectroscopy Of Livestock Emissions, D. I. Herman, L. C. Hutcherson, C. Weerasekara Et Al.

School of Natural Resources: Documents and Reviews

No abstract provided.


Estimating Abiotic Thresholds For Sagebrush Condition Class In The Western United States, Stephen Boyte, Bruce K. Wylie, Yingxin Gu, Donald J. Major Jan 2020

Estimating Abiotic Thresholds For Sagebrush Condition Class In The Western United States, Stephen Boyte, Bruce K. Wylie, Yingxin Gu, Donald J. Major

United States Geological Survey: Staff Publications

Sagebrush ecosystems of the western United States can transition from extended periods of relatively stable conditions to rapid ecological change if acute disturbances occur. Areas dominated by native sagebrush can transition from species-rich native systems to altered states where non-native annual grasses dominate, if resistance to annual grasses is low. The non-native annual grasses provide relatively little value to wildlife, livestock, and humans and function as fuel that increases fire frequency. The more land area covered by annual grasses, the higher the potential for fire, thus reducing the potential for native vegetation to reestablish, even when applying restoration treatments. Mapping …


Droughtscape, Winter 2019, Cory Matteson Jan 2020

Droughtscape, Winter 2019, Cory Matteson

DroughtScape: Quarterly Newsletter of the National Drought Mitigation Center (2007–2023)

Contents

From the Director

4th quarter climate summary

2019 year in review summary

4th quarter drought impact summary

2019 drought impact summary

New form helps ‘see more’ drought

Landscape photographers invited to submit photos

Helping Central and South America planning

Database includes more drought planning


Prairie Grouse Population Trends And Their Historical Drivers In The Nebraska Sandhills, Danielle Berger Jan 2020

Prairie Grouse Population Trends And Their Historical Drivers In The Nebraska Sandhills, Danielle Berger

School of Natural Resources: Dissertations, Theses, and Student Research

The Nebraska Game and Parks Commission (NGPC) has monitored greater prairie-chickens (Tympanuchus cupido pinnatus) and plains sharp-tailed grouse populations (Tympanuchus phasianellus jamesi), collectively known as prairie grouse, since the 1950s using spring breeding ground counts. My research modeled long-term, species-specific spatial and temporal trends of prairie grouse abundance and potential environmental drivers in the Sandhills of Nebraska using NGPC’s historical monitoring data. Prairie-chicken populations have increased since the 1950s while sharp-tailed grouse populations have remained stable or slightly declined. These population trends arise in the context of a dynamic landscape. I created indices representing raptor predation …


Mixed Ancestry From Wild And Domestic Lineages Contributes To The Rapid Expansion Of Invasive Feral Swine, Timothy J. Smyser, Michael A. Tabak, Chris Slootmaker, Michael S. Robeson Ii, Ryan S. Miller, Mirte Bosse, Hendrik-Jan Megens, Martien A.M. Groenen, Samuel Rezende Paiva, Danielle Assis De Faria, Harvey D. Blackburn, Brandon S. Schmit, Antoinette J. Piaggio Jan 2020

Mixed Ancestry From Wild And Domestic Lineages Contributes To The Rapid Expansion Of Invasive Feral Swine, Timothy J. Smyser, Michael A. Tabak, Chris Slootmaker, Michael S. Robeson Ii, Ryan S. Miller, Mirte Bosse, Hendrik-Jan Megens, Martien A.M. Groenen, Samuel Rezende Paiva, Danielle Assis De Faria, Harvey D. Blackburn, Brandon S. Schmit, Antoinette J. Piaggio

United States Department of Agriculture Wildlife Services: Staff Publications

Invasive alien species are a significant threat to both economic and ecological systems. Identifying the processes that give rise to invasive populations is essential for implementing effective control strategies. We conducted an ancestry analysis of invasive feral swine (Sus scrofa, Linnaeus, 1758), a highly destructive ungulate that is widely distributed throughout the contiguous United States, to describe introduction pathways, sources of newly emergent populations and processes contributing to an ongoing invasion. Comparisons of high-density single nucleotide polymorphism genotypes for 6,566 invasive feral swine to a comprehensive reference set of S. scrofa revealed that the vast majority of feral swine were …


The Role Of European Starlings (Sturnus Vulgaris) In The Dissemination Of Multidrug- Resistant Escherichia Coli Among Concentrated Animal Feeding Operations, Jeffrey C. Chandler, Jennifer E. Anders, Nicolas A. Blouin, James C. Carlson, Jeffrey T. Lejeune, Lawrence D. Goodridge, Baolin Wang, Leslie A. Day, Anna M. Mangan, Dustin A. Reid, Shannon M. Coleman, Matthew W. Hopken, Bledar Bisha Jan 2020

The Role Of European Starlings (Sturnus Vulgaris) In The Dissemination Of Multidrug- Resistant Escherichia Coli Among Concentrated Animal Feeding Operations, Jeffrey C. Chandler, Jennifer E. Anders, Nicolas A. Blouin, James C. Carlson, Jeffrey T. Lejeune, Lawrence D. Goodridge, Baolin Wang, Leslie A. Day, Anna M. Mangan, Dustin A. Reid, Shannon M. Coleman, Matthew W. Hopken, Bledar Bisha

United States Department of Agriculture Wildlife Services: Staff Publications

Antimicrobial use in livestock production is a driver for the development and proliferation of antimicrobial resistance (AMR). Wildlife interactions with livestock, acquiring associated AMR bacteria and genes, and wildlife’s subsequent dispersal across the landscape are hypothesized to play an important role in the ecology of AMR. Here, we examined priority AMR phenotypes and genotypes of Escherichia coli isolated from the gastrointestinal tracts of european starlings (Sturnus vulgaris) found on concentrated animal feeding operations (CAFOs). European starlings may be present in high numbers on cAfos (>100,000 birds), interact with urban environments, and can migrate distances exceeding 1,500 km in North …


Reporting The Limits Of Detection And Quantification For Environmental Dna Assays, Katy E. Klymus, Christopher M. Merkes, Michael J. Allison, Caren S. Goldberg, Caren C. Helbing, Margaret E. Hunter, Craig A. Jackson, Richard F. Lance, Anna M. Mangan, Emy M. Monroe, Antoinette J. Piaggio, Joel P. Stokdyk, Chris C. Wilson, Catherine A. Richter Jan 2020

Reporting The Limits Of Detection And Quantification For Environmental Dna Assays, Katy E. Klymus, Christopher M. Merkes, Michael J. Allison, Caren S. Goldberg, Caren C. Helbing, Margaret E. Hunter, Craig A. Jackson, Richard F. Lance, Anna M. Mangan, Emy M. Monroe, Antoinette J. Piaggio, Joel P. Stokdyk, Chris C. Wilson, Catherine A. Richter

United States Department of Agriculture Wildlife Services: Staff Publications

Background: Environmental DNA (eDNA) analysis is increasingly being used to detect the presence and relative abundance of rare species, especially invasive or imperiled aquatic species. The rapid progress in the eDNA field has resulted in numerous studies impacting conservation and management actions. However, standardization of eDNA methods and reporting across the field is yet to be fully established, with one area being the calculation and interpretation of assay limit of detection (LOD) and limit of quantification (LOQ).

Aims: Here, we propose establishing consistent methods for determining and reporting of LOD and LOQ for single‐species quantitative PCR (qPCR) eDNA studies.

Materials …


Safety, Immunogenicity, And Efficacy Of Intramuscular And Oral Delivery Of Era-G333 Recombinant Rabies Virus Vaccine To Big Brown Bats (Eptesicus Fuscus), Amy T. Gilbert, Xianfu Wu, Felix R. Jackson, Richard Franka, Gary F. Mccracken, Charles E. Rupprecht Jan 2020

Safety, Immunogenicity, And Efficacy Of Intramuscular And Oral Delivery Of Era-G333 Recombinant Rabies Virus Vaccine To Big Brown Bats (Eptesicus Fuscus), Amy T. Gilbert, Xianfu Wu, Felix R. Jackson, Richard Franka, Gary F. Mccracken, Charles E. Rupprecht

United States Department of Agriculture Wildlife Services: Staff Publications

Attenuated strains of rabies virus (RABV) have been used for oral vaccination of wild carnivores in Europe and North America. However, some RABV vaccines caused clinical rabies in target animals. To improve the safety of attenuated RABV as an oral vaccine for field use, strategies using selection of escape mutants under monoclonal antibody neutralization pressure and reverse genetics–defined mutations have been used. We tested the safety, immunogenicity, and efficacy of one RABV construct, ERA-g333, developed with reverse genetics by intramuscular (IM) or oral (PO) routes in big brown bats (Eptesicus fuscus). Twenty-five bats received 5×106 mouse intracerebral …


Psychological Drivers Of Risk-Reducing Behaviors To Limit Human–Wildlife Conflict, Stacy A. Lischka, Tara L. Teel, Heather E. Johnson, Courtney Larson, Stewart Breck, Kevin R. Crooks Jan 2020

Psychological Drivers Of Risk-Reducing Behaviors To Limit Human–Wildlife Conflict, Stacy A. Lischka, Tara L. Teel, Heather E. Johnson, Courtney Larson, Stewart Breck, Kevin R. Crooks

United States Department of Agriculture Wildlife Services: Staff Publications

Conflicts between people and wild animals are increasing globally, often with serious consequences

for both. Local regulations or ordinances are frequently used to promote human behaviors that minimize these conflicts (risk-reducing behaviors), but compliance with ordinances can be highly variable. While efforts to increase compliance could be improved through applications of conservation psychology, little is known about the relative influence of different factors motivating compliance. Using concepts from psychology and risk theory, we conducted a longitudinal study pairing data from mail surveys with direct observations of compliance with a wildlife ordinance requiring residents to secure residential garbage from black bears …


Timing And Extent Of Crop Damage By Wild Pigs (Sus Scrofa Linnaeus) To Corn And Peanut Fields, C. M. Boyce, Kurt C. Vercauteren, James C. Beasley Jan 2020

Timing And Extent Of Crop Damage By Wild Pigs (Sus Scrofa Linnaeus) To Corn And Peanut Fields, C. M. Boyce, Kurt C. Vercauteren, James C. Beasley

United States Department of Agriculture Wildlife Services: Staff Publications

The global expansion of wild pigs over the last few decades has resulted in an increase in extent and distribution of damages to crops, placing a growing strain on agricultural producers and land managers. Despite the extent of wild pig damage to agriculture, there is little data regarding timing and spatial variability of damage to corn (Zea mays Linnaeus) and we found no data regarding the effect of these factors on peanuts (Arachis hypogaea Linnaeus). Our objective was to determine the timing and extent of wild pig damage to corn and peanut fields, as well as the extent …


Validation Of A Screening Method For The Detection Of Colistin-Resistant E. Coli Containing Mcr-1 In Feral Swine Feces, Jeffrey C. Chandler, Alan B. Franklin, S. N. Bevins, Kevin T. Bentler, Jonas Bonnedahl, Christina A. Ahlstrom, Bledar Bisha, Susan A. Shriner Jan 2020

Validation Of A Screening Method For The Detection Of Colistin-Resistant E. Coli Containing Mcr-1 In Feral Swine Feces, Jeffrey C. Chandler, Alan B. Franklin, S. N. Bevins, Kevin T. Bentler, Jonas Bonnedahl, Christina A. Ahlstrom, Bledar Bisha, Susan A. Shriner

United States Department of Agriculture Wildlife Services: Staff Publications

A method was developed and validated for the detection of colistin-resistant Escherichia coli containing mcr-1 in the feces of feral swine. Following optimization of an enrichment method using EC broth supplemented with colistin (1 μg/mL) and vancomycin (8 μg/mL), aliquots derived from 100 feral swine fecal samples were spiked with of one of five different mcr-1 positive E. coli strains (between 100 and 104 CFU/g), for a total of 1110 samples tested. Enrichments were then screened using a simple boil-prep and a previously developed real-time PCR assay for mcr-1 detection. The sensitivity of the method was determined in …