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Articles 751 - 780 of 1357
Full-Text Articles in Natural Resources and Conservation
The Feasibility Of Producing Adequate Feedstock For Year-Round Cellulosic Ethanol Production In An Intensive Agricultural Fuelshed, Daniel R. Uden, Robert B. Mitchell, Craig R. Allen, Qingfeng Guan, Tim D. Mccoy
The Feasibility Of Producing Adequate Feedstock For Year-Round Cellulosic Ethanol Production In An Intensive Agricultural Fuelshed, Daniel R. Uden, Robert B. Mitchell, Craig R. Allen, Qingfeng Guan, Tim D. Mccoy
Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications
To date, cellulosic ethanol production has not been commercialized in the United States. However, government mandates aimed at increasing second-generation biofuel production could spur exploratory development in the cellulosic ethanol industry. We conducted an in-depth analysis of the fuelshed surrounding a starch-based ethanol plant near York, Nebraska that has the potential for cellulosic ethanol production. To assess the feasibility of supplying adequate biomass for year-round cellulosic ethanol production from residual maize (Zea mays) stover and bioenergy switchgrass (Panicum virgatum) within a 40-km road network service area of the existing ethanol plant, we identified ∼14,000 ha of marginally productive cropland within …
Survival And Behavior Of Chinese Mystery Snails (Bellamya Chinensis) In Response To Simulated Water Body Drawdowns And Extended Air Exposure, Kody M. Unstad, Daniel R. Uden, Craig R. Allen, Noelle M. Chaine, Danielle M. Haak, Robert A. Kill, Kevin L. Pope, Bruce J. Stephen, Alec Wong
Survival And Behavior Of Chinese Mystery Snails (Bellamya Chinensis) In Response To Simulated Water Body Drawdowns And Extended Air Exposure, Kody M. Unstad, Daniel R. Uden, Craig R. Allen, Noelle M. Chaine, Danielle M. Haak, Robert A. Kill, Kevin L. Pope, Bruce J. Stephen, Alec Wong
Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications
Nonnative invasive mollusks degrade aquatic ecosystems and induce economic losses worldwide. Extended air exposure through water body drawdown is one management action used for control. In North America, the Chinese mystery snail (Bellamya chinensis) is an invasive aquatic snail with an expanding range, but eradication methods for this species are not well documented. We assessed the ability of B. chinensis to survive different durations of air exposure, and observed behavioral responses prior to, during, and following desiccation events. Individual B. chinensis specimens survived air exposure in a laboratory setting for > 9 weeks, and survivorship was greater among adults …
Enamel-Based Mark Performance For Marking Chinese Mystery Snail Bellamya Chinensis, Alec Wong, Craig R. Allen, Noelle M. Hart, Danielle M. Haak, Kevin L. Pope, Nicholas A. Smeenk, Bruce J. Stephen, Daniel R. Uden
Enamel-Based Mark Performance For Marking Chinese Mystery Snail Bellamya Chinensis, Alec Wong, Craig R. Allen, Noelle M. Hart, Danielle M. Haak, Kevin L. Pope, Nicholas A. Smeenk, Bruce J. Stephen, Daniel R. Uden
Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications
The exoskeleton of gastropods provides a convenient surface for carrying marks, and in the interest of improving future marking methods our laboratory assessed the performance of an enamel paint. The endurance of the paint was also compared to other marking methods assessed in the past. We marked the shells of 30 adult Chinese mystery snails Bellamya chinensis and held them in an aquarium for 181 days. We observed no complete degradation of any enamel-paint mark during the 181 days. The enamel-paint mark was superior to a nail-polish mark, which lasted a median of 100 days. Enamel-paint marks also have a …
Mortality Estimate Of Chinese Mystery Snail, Bellamya Chinensis (Reeve, 1863) In A Nebraska Reservoir, Danielle M. Haak, Noelle M. Chaine, Bruce J. Stephen, Alec Wong, Craig R. Allen
Mortality Estimate Of Chinese Mystery Snail, Bellamya Chinensis (Reeve, 1863) In A Nebraska Reservoir, Danielle M. Haak, Noelle M. Chaine, Bruce J. Stephen, Alec Wong, Craig R. Allen
Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications
The Chinese mystery snail (Bellamya chinensis) is an aquatic invasive species found throughout the USA. Little is known about this species’ life history or ecology, and only one population estimate has been published, for Wild Plum Lake in southeast Nebraska. A recent die-off event occurred at this same reservoir and we present a mortality estimate for this B. chinensis population using a quadrat approach. Assuming uniform distribution throughout the newly-exposed lake bed (20,900 m2), we estimate 42,845 individuals died during this event, amounting to approximately 17% of the previously-estimated population size of 253,570. Assuming uniform distribution throughout all …
Scenarios Of Bioenergy Development Impacts On Regional Groundwater Withdrawals, Daniel R. Uden, Craig R. Allen, Rob B. Mitchell, Qingfeng Guan, Tim D. Mccoy
Scenarios Of Bioenergy Development Impacts On Regional Groundwater Withdrawals, Daniel R. Uden, Craig R. Allen, Rob B. Mitchell, Qingfeng Guan, Tim D. Mccoy
Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications
Irrigation increases agricultural productivity, but it also stresses water resources (Huffaker and Hamilton 2007). Drought and the potential for drier conditions resulting from climate change could strain water supplies in landscapes where human populations rely on finite groundwater resources for drinking, agriculture, energy, and industry (IPCC 2007). For instance, in the North American Great Plains, rowcrops are utilized for livestock feed, food, and bioenergy production (Cassman and Liska 2007), and a large portion is irrigated with groundwater from the High Plains aquifer system (McGuire 2011). Under projected future climatic conditions, greater crop water use requirements and diminished groundwater recharge rates …
Rainwater Basin Wetland Inundation Decision Support System Guide, Christopher F. Jorgensen, Andy A. Bishop, Daniel R. Uden, Roger Grosse
Rainwater Basin Wetland Inundation Decision Support System Guide, Christopher F. Jorgensen, Andy A. Bishop, Daniel R. Uden, Roger Grosse
Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications
The Rainwater Basin region of south-central Nebraska, U.S.A., is a critical stopover location for migratory waterfowl, waterbirds and shorebirds traveling along the Central Flyway. The ephemeral playa wetlands in the region serve as spring staging areas, providing critical habitat for migrating birds to rest, feed and establish pairs before resuming their northward migration to the breeding grounds. Collectively, the playa wetlands found in the region form the Rainwater Basin Wetland Complex, encompassing a 6,150 square-mile area of rolling loess plains that extend over 21 counties. Analyses of the State’s historic soil surveys (1910-1917), National Wetland Inventory (NWI; 1980-1982; U.S. Geological …
Effects Of Simulated Angler Capture And Live-Release Tournaments On Walleye Survival, John H. Loomis, Harold L. Schramm Jr., Bruce Vondracek, Patrick D. Gerard, Christopher J. Chizinski
Effects Of Simulated Angler Capture And Live-Release Tournaments On Walleye Survival, John H. Loomis, Harold L. Schramm Jr., Bruce Vondracek, Patrick D. Gerard, Christopher J. Chizinski
Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications
We examined the effects of acclimation water temperature, live-well (LW) water temperature, and LW dissolved oxygen (DO) concentration on survival of adult Walleyes Sander vitreus subjected to simulated tournament conditions (angling, LW confinement, and weigh-in procedures) under controlled laboratory conditions. We tested three acclimation temperatures (12, 18, and 24◦C), and three LWtemperature differentials (Delta T=−4, 0, and +4◦C) were tested at each acclimation temperature. Survival was monitored after 8 h of LW confinement and during a 5-d retention period in 1,700-L tanks. None of the Walleyes that were acclimated to 24◦C and subjected to simulated tournament procedures survived the 5-d …
2013 Nebraska Groundwater Quality Monitoring Report
2013 Nebraska Groundwater Quality Monitoring Report
Nebraska Department of Environmental Quality: Reports
The 2001 Nebraska Legislature passed LB329 (Neb. Rev. Stat. §46-1304) which, in part, directed the Nebraska Department of Environmental Quality (NDEQ) to report on groundwater quality monitoring in Nebraska. Reports have been issued annually since December 2001. The text of the statute applicable to this report follows: “The Department of Environmental Quality shall prepare a report outlining the extent of ground water quality monitoring conducted by natural resources districts during the preceding calendar year. The department shall analyze the data collected for the purpose of determining whether or not ground water quality is degrading or improving and shall present the …
Using Gis To Delineate Headwater Stream Origins In The Appalachian Coal-Belt Region Of Kentucky, Jonathan A. Villines
Using Gis To Delineate Headwater Stream Origins In The Appalachian Coal-Belt Region Of Kentucky, Jonathan A. Villines
Theses and Dissertations--Biosystems and Agricultural Engineering
Human activity such as surface mining can have substantial impacts on the natural environment. Performing a Cumulative Hydrologic Impact Assessment (CHIA) of such impacts on surface water systems requires knowing the location and extent of these impacted streams. The Jurisdictional Determination (JD) of a stream’s protected status under the Clean Water Act (CWA) involves locating and classifying streams according to their flow regime: ephemeral, intermittent, or perennial. Due to their often remote locations and small size, taking a field inventory of headwater streams for surface mining permit applications or permit reviews is challenging. A means of estimating headwater stream location …
Acequias, Brigette Buynak, Jerold Widdison, Darcy S. Bushnell
Acequias, Brigette Buynak, Jerold Widdison, Darcy S. Bushnell
Water Matters!
Acequias are community irrigation systems in the villages and pueblos of New Mexico. They have deep roots in two ancient traditions—Pueblo Indian and Spanish. The Pueblos collected and shared water for centuries before the arrival of Spanish colonists in 1598. The Spanish settlers brought technical knowledge and institutional frameworks for governing irrigation systems, which originated in the Moors’ seven-century occupation of Spain. Both traditions remain important to an understanding of New Mexico’s acequia heritage and the continuing relevance of these “water democracies.”Today, these traditions must meld with state law as the legislature has provided that acequias are “political subdivisions” or …
American Indian Water Rights, Michael Osborn, Darcy S. Bushnell
American Indian Water Rights, Michael Osborn, Darcy S. Bushnell
Water Matters!
Pueblos and tribal reservations are located within most of the larger stream systems in New Mexico. Each has claims to rights to use the water in its stream. In New Mexico, Indian rights are significant because of their early priority dates, because of the large amounts of water rights claimed, or both. In some instances, such claims have the potential to displace a significant number of junior water rights.
Common law theories or doctrines pertaining to Indians continue to be judicially refined and to evolve so that discussing the nature and extent of “Indian water rights” is a complex topic.
Groundwater, Darcy S. Bushnell, Diego Urbina
Groundwater, Darcy S. Bushnell, Diego Urbina
Water Matters!
Since the late nineteenth century, New Mexicans have been developing the state’s groundwater resources. From hand-dug wells to proposed wells that could penetrate to 12,000 feet, residents have sought sources to supplement and replace surface water. The state relies upon groundwater to supply almost 50 percent of its needs.
As the population grows and drought intensifies, groundwater sources are tapped with increasing urgency. Limited steps are being taken to preserve groundwater through conservation, groundwater recharge, and regulation.
State And Regional Water Planning, Brigette Buynak, Susan Kelly, Sarah Armstrong
State And Regional Water Planning, Brigette Buynak, Susan Kelly, Sarah Armstrong
Water Matters!
A statewide water planning effort was initiated by the New Mexico legislature in the 2003 session. The Interstate Stream Commission (ISC),in collaboration with the Office of the State Engineer (OSE) and the Water Trust Board, was tasked with preparing and implementing a comprehensive state water plan. Regional water planning had begun much earlier, prompted by a lawsuit that El Paso filed against New Mexico in 1983, El Paso v. Reynolds.
The State Water Plan Act of 2003 (Act) was intended to promote stewardship of the state’s water resources and to establish clear policies and strategies for management of the state’s …
Community Water Systems, Joanne Hilton, Susan Kelly, Sarah Armstrong
Community Water Systems, Joanne Hilton, Susan Kelly, Sarah Armstrong
Water Matters!
Apart from the major cities along the Rio Grande corridor, much of New Mexico remains relatively rural. Recent studies estimate a 2013population of around 2,085,500 statewide. In the state fiscal year 2011,about 1,836,000 people, or 88 percent of New Mexico’s population obtain their water from community water systems. Approximately 284,000 people, or about14 percent of the population, receive their drinking water from community water systems serving fewer than 5,000 people. As of 2012, there are 1,148 public water systems that provide drinking water in New Mexico. Of these systems, 593are community water systems; of these, 546 serve fewer than 5,000 …
Deep Water Regulation, Paul Bossert, Kari Olson
Deep Water Regulation, Paul Bossert, Kari Olson
Water Matters!
With most of the surface water in New Mexico fully appropriated and with groundwater sources being drawn down and becoming less reliable, the search for new sources of water is reaching further and further afield of traditional sources and methods. Water wells deeper than 2,000 feet have been rare due to the expense of deep drilling and the uncertainty of finding potable water. Yet the combined circumstances of advances in hydrology and the escalating demand for new water have driven the search for water deeper than was previously considered practical.
Water Marketing, Jeremy Oat, Laura Paskus
Water Marketing, Jeremy Oat, Laura Paskus
Water Matters!
Water doesn’t just flow around New Mexico in streams and rivers: it also moves around on paper. Since all of the state’s surface-water and most of its groundwater have already been allocated, the only way for cities, developers, or conservation organizations to find new water supplies is to buy and transfer water rights from old uses and places to new uses and places. The N.M. Office of the State Engineer (OSE) approves each of these transfers, most of which are relatively small, but the numbers can add up over time. Between 1982 and 2011, for instance, 21,000 acre-feet of Middle …
Water Quality Regulation, Joanne Hilton, Susan Kelly, James Hogan, Kimberly Kirby, Jerry Schoeppner
Water Quality Regulation, Joanne Hilton, Susan Kelly, James Hogan, Kimberly Kirby, Jerry Schoeppner
Water Matters!
While many of the water issues in New Mexico center around having an adequate supply of water, the quality of the water is just as important as the quantity in supplying water for drinking and other uses that rely on clean water. Protecting water quality is financially more feasible than conducting expensive cleanup programs. New Mexico has a strong interest in water quality regulation to protect public health and the environment and to minimize expenditures for mitigation of contaminated supplies. Water quality is a difficult subject to navigate; there is a complex web of statutes and agency involvement. This paper …
Water Litigation In The Lower Rio Grande, Darcy S. Bushnell
Water Litigation In The Lower Rio Grande, Darcy S. Bushnell
Water Matters!
The water allocation issues are hotly contested in south-central New Mexico and the surrounding area. Today, the river and those who depend on it face more administrative challenges in the face of shrinking water supplies and increased population. These challenges have given rise to two ongoing lawsuits: the Lower Rio Grande Adjudication,New Mexico v. EBID, et al., 96-CV-888 (1996) (N.M. v. EBID) in the New Mexico Third Judicial District Court (adjudication court) and the New Mexico v. United States,et al., D.N.M. 11-CV-691 (2011) (N.M. v.U.S.) in United States District Court of New Mexico (U.S. District Court).
Eastern New Mexico Rural Water System (Ute Pipeline Project), Jerold Widdison, Paul Van Gulick, Darcy S. Bushnell
Eastern New Mexico Rural Water System (Ute Pipeline Project), Jerold Widdison, Paul Van Gulick, Darcy S. Bushnell
Water Matters!
The Ute Pipeline Project (Project), officially known as the Eastern New Mexico Rural Water System (ENMRWS),is a 151-mile-long pipeline project to provide a sustainable municipal and industrial water supply for several eastern New Mexico communities and a military base. The Congress authorized major federal funding for the Ute Pipeline in the Omnibus Public Land Management Act of 2009. This important milestone for the project was reached after about 45 years of effort.Attention now shifts to myriad details involved in actually constructing, financing,and administering the project.
The Rio Grande As An International River, Margaret J. Vick
The Rio Grande As An International River, Margaret J. Vick
Water Matters!
The Rio Grande is divided into two major river reaches and has different legal regimes for each. New Mexico is primarily concerned with the Rio Grande from the headwaters in Colorado to Ft. Quitman in Texas, a distance of approximately 670 miles. This section of the river is the subject of the1906 Rio Grande Convention (Treaty) between the United States and Mexico. The lower section of the Rio Grande from Ft. Quitman to the Gulf of Mexico is the subject of the 1944 Rivers Treaty between the United States and Mexico; the 1944 Rivers Treaty also includes the Colorado and …
Nevada Interagency Volunteer Program: Helping Hands Across Public Lands—Phase Ii: Final Project Report, December 31, 2012, Margaret N. Rees
Nevada Interagency Volunteer Program: Helping Hands Across Public Lands—Phase Ii: Final Project Report, December 31, 2012, Margaret N. Rees
Get Outdoors Nevada
- Maintained a central clearinghouse created for Southern Nevada public lands volunteers, including: ─ an online volunteer data-management application (Volgistics) adopted and customized for the program and populated with 8,500 active and 961 mailing list volunteer records ─ a community outreach Web site that currently averages 8,230 pages viewed per month since July 2007 and has aided in the recruitment of 11,943 volunteers ─ updated website to word press for sustainability ─ branding development and marketing and outreach tool creation
- Updated Standard Operating Procedures (SOPs) for all areas of Interagency Volunteer Program Management—recruitment, training, recognition, retention of volunteers, event planning, and …
Interactions Of Zooplankton And Phytoplankton With Cyanobacteria, Rebecca Alexander
Interactions Of Zooplankton And Phytoplankton With Cyanobacteria, Rebecca Alexander
School of Natural Resources: Dissertations, Theses, and Student Research
Cyanobacteria are a major concern in Nebraska reservoirs and are capable of producing toxins that can cause skin irritations and gastrointestinal problems, as well as affect the nervous system. It is important to determine the mechanisms that can cause cyanobacteria blooms due to the effect they can have on human health. The interaction of zooplankton and other phytoplankton groups with cyanobacteria is important because there is a biological component in surface waters that should be taken into consideration along with the physical and chemical parameters that have been noted to promote cyanobacteria. For example, zooplankton have the ability to alter …
Mortality Assessment Of Redwood And Mixed Conifer Forest Types In Santa Cruz County Following Wildfire, Steve R. Auten
Mortality Assessment Of Redwood And Mixed Conifer Forest Types In Santa Cruz County Following Wildfire, Steve R. Auten
Master's Theses
On August 12, 2009, the Lockheed Fire ignited the west slope of the Santa Cruz Mountains burning approximately 7,819 acres. Foresters and other land managers were left with challenging decisions on how to evaluate tree mortality. Big Creek Lumber Company, California Polytechnic State University, San Luis Obispo (Cal Poly)’s Swanton Pacific Ranch (SPR), and other resource professionals familiar with this region teamed up to develop a method for evaluating damage and thereby mortality for redwood, California nutmeg, live oak, tanoak, California bay, Pacific madrone, big leaf maple, Douglas-fir, Monterey pine, and knobcone pine. Quantitative damage criteria were used to design …
Drinking Water Resource Directory, New England Environmental Finance Center
Drinking Water Resource Directory, New England Environmental Finance Center
Sustainable Communities Capacity Building
This document is intended to help local and regional planning agencies, and their constituent water utilities, integrate drinking water infrastructure planning and investments into plans for sustainable development. Resources listed here provide guidance on making land use decisions that protect water resources, setting adequate and sustainable drinking water rates, controlling water loss, funding water infrastructure projects, and managing water utilities.
The directory was developed by the Environmental Finance Center Network through the Capacity Building for Sustainable Communities program funded by the U.S. Department of Housing and Urban Development and the Environmental Protection Agency. Through this program, EFCN is providing capacity …
Droughtscape, Fall 2012, National Drought Mitigation Center
Droughtscape, Fall 2012, National Drought Mitigation Center
DroughtScape: Quarterly Newsletter of the National Drought Mitigation Center (2007–2023)
Contents
Director’s report
Updated resources
Drought outlook and review
2012 and past droughters
Summer impacts summary
Urban planners and drought
USDM wins CLEAN seal
Meixiu Yu's Chinese SPEI
Ethiopian project
Mexican VegDRI
Drought monitor sculpture
Economics Of Nuclear Power And Climate Change Mitigation Policies, Nico Bauer, Robert J. Brecha, Gunnar Luderer
Economics Of Nuclear Power And Climate Change Mitigation Policies, Nico Bauer, Robert J. Brecha, Gunnar Luderer
Physics Faculty Publications
The events of March 2011 at the nuclear power complex in Fukushima, Japan, raised questions about the safe operation of nuclear power plants, with early retirement of existing nuclear power plants being debated in the policy arena and considered by regulators. Also, the future of building new nuclear power plants is highly uncertain. Should nuclear power policies become more restrictive, one potential option for climate change mitigation will be less available. However, a systematic analysis of nuclear power policies, including early retirement, has been missing in the climate change mitigation literature. We apply an energy economy model framework to derive …
A Comparison Of Qualitative And Quantitative Ecological Assessment Methods Of Mangroves In Southwestern Madagascar, Sondra Winders
A Comparison Of Qualitative And Quantitative Ecological Assessment Methods Of Mangroves In Southwestern Madagascar, Sondra Winders
Independent Study Project (ISP) Collection
Mangroves are unique, tropical, intertidal forests that, among many other important functions, serve as large carbon sinks for the sequestration of atmospheric CO2. This project qualitatively assessed the mangrove forest of Honko Mangrove Conservation and Education (Tulear, Madagascar) in conjunction with Blue Ventures for a proposed Plan Vivo carbon stock project. The qualitative results were then compared with quantitative measurements in order to determine the most effective method of ecological assessment. Sixty-three stands of mangrove forest covering 9.72km2 were identified and mapped. Level of harvest, species composition, density, canopy cover, dominant height, dominant DBH, forest condition and composition were compared …
Nevada Interagency Volunteer Program: Helping Hands Across Public Lands - Phase Ii: Quaterly Progress Report, Period Covering July 1, 2012 To September 30, 2012, Margaret N. Rees
Nevada Interagency Volunteer Program: Helping Hands Across Public Lands - Phase Ii: Quaterly Progress Report, Period Covering July 1, 2012 To September 30, 2012, Margaret N. Rees
Get Outdoors Nevada
- The number of records in the volunteer database is 8,511, an increase from 8,308 in July.
- Results show an average of 1,094 visits per month, and an average of 2,729 pages viewed per month.
- Get Outdoors Nevada supp01ied 6 volunteer events in a variety of ways. These events utilized 214 volunteers whom contributed approximately 856 hours of service.
- The team continues planning an alternative bus tour.
- The team rolled out their media plan .
- The IVP team has continued planning for the annual volunteer recognition banquet.
- No volunteer trainings were conducted this quarter, but two are planned for next quarter.
Limnological Assistance For Lake Mead National Recreation Area: Quarterly Report, Period Ending September 30, 2012, Margaret N. Rees
Limnological Assistance For Lake Mead National Recreation Area: Quarterly Report, Period Ending September 30, 2012, Margaret N. Rees
Limnological Studies
Continuation of Project 1
- The research effort titled Growth of adult quagga mussels within Las Vegas Wash/Las Vegas Bay is ongoing.
Continuation of Project 2
- Additional content was contributed to the future NPS-funded U.S. Geological Survey Circular ‘state-of-the-science’ report for Lakes Mead and Mohave. UNLV performed a new co-editing and coordinating task related to this product as the major focus of this quarter’s effort; the master version of this report is on schedule for delivery to the editorial team during the first week of October 2012.
Modification Activities:
Extended Short-term Continued Limnological Assistance
Quagga Mussel Objectives:
- Most juvenile/adult quagga mussel …
Bait Effects In Sampling Coral Reef Fish Assemblages With Stereo-Bruvs, Stacey R. Dorman, Euan S. Harvey, Stephen J. Newman
Bait Effects In Sampling Coral Reef Fish Assemblages With Stereo-Bruvs, Stacey R. Dorman, Euan S. Harvey, Stephen J. Newman
Fisheries Research Articles
Baited underwater video techniques are increasingly being utilised for assessing and monitoring demersal fishes because they are: 1) non extractive, 2) can be used to sample across multiple habitats and depths, 3) are cost effective, 4) sample a broader range of species than many other techniques, 5) and with greater statistical power. However, an examination of the literature demonstrates that a range of different bait types are being used. The use of different types of bait can create an additional source of variability in sampling programs. Coral reef fish assemblages at the Houtman Abrolhos Islands, Western Australia, were sampled using …