Natural Resource Economics Commons^™

Surveillance Of Plasticizers, Bisphenol A, Steroids And Caffeine In Surface Water Of River Ganga And Sundarban Wetland Along The Bay Of Bengal: Occurrence, Sources, Estrogenicity Screening And Ecotoxicological Risk Assessment, Paromita Chakraborty, Nancy W. Shappell, Moitraiyee Mukhopadhyay, Sathaporn Onanong, Daniel D. Snow

Daugherty Water for Food Global Institute: Faculty Publications

The transboundary River Ganga serves as a conduit for meltwater from the Himalayas and is a major freshwater source for two thirds of Indian population before emptying into the Sundarban Delta, the largest estuary in the Bay of Bengal. Endocrine disrupting compounds (EDCs) such as phthalic acid esters (PAEs) and bisphenol A (BPA) used as organic plastic additives can pollute the aquatic environment receiving plastic litter. Hence, we have investigated these EDCs in water samples from Ganga and Sundarban wetland of India. Since these compounds exhibit estrogenic potential, we have further measured steroids and evaluated the estrogenic activity (estradiol equivalents, …

Transmission Routes Of The Microbiome And Resistome From Manure To Soil And Lettuce, Yuepeng Sun, Daniel D. Snow, Harkamal Walia, Xu Li

Daugherty Water for Food Global Institute: Faculty Publications

The land application of animal manure can introduce manure microbiome and resistome to croplands where food crops are grown. The objective of this study was to characterize the microbiome and resistome on and in the leaves of lettuce grown in manured soil and identify the main transmission routes of microbes and antibiotic resistance genes (ARGs) from soil to the episphere and endosphere of lettuce. Shotgun metagenomic results show that manure application significantly altered the composition of the microbiome and resistome of surface soil. SourceTracker analyses indicate that manure and original soil were the main source of the microbiome and resistome …

Developing A Tourism Opportunity Index Regarding The Prospective Of Overtourism In Nepal, Susan Phuyal

Graduate Theses/Dissertations

This research explores Nepal's overtourism scenario based on the capacity of a locality to manage sustainable tourism practices. Environmental degradation, local infrastructure degradation, negative tourist experience and local resident responses regarding visitors are the four main variables used in this study to analyze overtourism. In order to analyze the case study of overtourism, we select the three top touristic cities of Nepal, Kathmandu, Pokhara, and Chitwan based on the number of annual visitors. Nepal's case analysis of overtourism conditions reviews the overall threat of over-tourism and establishes a metric by which tourism can be viewed as potentially detrimental to sustainability. …

Aspergillus Niger Decreases Bioavailability Of Arsenic(V) Via Biotransformation Of Manganese Oxide Into Biogenic Oxalate Minerals, Bence Farkas, Marek Kolenčík, Miroslav Hain, Edmund Dobročka, Gabriela Kratošová, Marek Bujdoš, Huan Feng, Yang Deng, Qian Yu, Ramakanth Illa, B. Ratna Sunil, Hyunjung Kim, Peter Matúš, Martin Urík

Department of Earth and Environmental Studies Faculty Scholarship and Creative Works

The aim of this work was to evaluate the transformation of manganese oxide (hausmannite) by microscopic filamentous fungus Aspergillus niger and the effects of the transformation on mobility and bioavailability of arsenic. Our results showed that the A. niger strain CBS 140837 greatly affected the stability of hausmannite and induced its transformation into biogenic crystals of manganese oxalates—falottaite and lindbergite. The transformation was enabled by fungal acidolysis of hausmannite and subsequent release of manganese ions into the culture medium. While almost 45% of manganese was bioextracted, the arsenic content in manganese precipitates increased throughout the 25-day static cultivation of fungus. …

Satellite-Based Monitoring Of Irrigation Water Use: Assessing Measurement Errors And Their Implications For Agricultural Water Management Policy, T. Foster, Taro Mieno, Nicholas Brozovic

Daugherty Water for Food Global Institute: Faculty Publications

Reliable accounting of agricultural water use is critical for sustainable water management. However, the majority of agricultural water use is not monitored, with limited metering of irrigation despite increasing pressure on both groundwater and surface water resources in many agricultural regions worldwide. Satellite remote sensing has been proposed as a low-cost and scalable solution to fill widespread gaps in monitoring of irrigation water use in both developed and developing countries, bypassing the technical, socioeconomic, and political challenges that to date have constrained in situ metering. In this paper, we show through a systematic meta-analysis that the relative accuracy of different …

Agate Fossil Beds National Monument, Paleontological Resources Management Plan (Public Version), Scott Kottkamp, Vincent L. Santucci, Justin S. Tweet, Jessica De Smet, Ellen Stark

United States National Park Service: Publications

Executive Summary

Since Agate Springs Ranch was founded by James H. Cook in 1887, exquisite examples of transitional Miocene mammalian fauna have been found along this stretch of the Niobrara River valley. Collectively these paleontological discoveries, along with the existing archeological and historical Native American collection, were the basis for establishing Agate Fossil Beds National Monument (AGFO) as a unit of the National Park System (NPS). The fossil remains from the Harrison and Anderson Ranch formations span a short, but important, time period within the Miocene Epoch. AGFO has provided science with an intimate look into North American mammalian evolution …

Assessment Of Switchgrass-Based Bioenergy Supply Using Gis-Based Fuzzy Logic And Network Optimization In Missouri (U.S.A.), Gia Nguyen, Erik Lyttek, Pankaj Lal, Taylor Wieczerak, Pralhad Burli

Department of Earth and Environmental Studies Faculty Scholarship and Creative Works

Bioenergy has been globally recognized as one of the sustainable alternatives to fossil fuels. An assured supply of biomass feedstocks is a crucial bottleneck for the bioenergy industry emanating from uncertainties in land-use changes and future prices. Analytical approaches deriving from geographical information systems (GIS)-based analysis, mathematical modeling, optimization analyses, and empirical techniques have been widely used to evaluate the potential for bioenergy feedstock. In this study, we propose a three-phase methodology integrating fuzzy logic, network optimization, and ecosystem services assessment to estimate potential bioenergy supply. The fuzzy logic analysis uses multiple spatial criteria to identify suitable biomass cultivating regions. …

Foliar Application Of Low Concentrations Of Titanium Dioxide And Zinc Oxide Nanoparticles To The Common Sunflower Under Field Conditions, Marek Kolenčík, Dávid Ernst, Martin Urík, Ľuba Ďurišová, Marek Bujdoš, Martin Šebesta, Edmund Dobročka, Samuel Kšiňan, Ramakanth Illa, Qian Yu, Huan Feng, Ivan Černý, Veronika Holišová, Gabriela Kratošová

Department of Earth and Environmental Studies Faculty Scholarship and Creative Works

Nano-fertilisers have only recently been introduced to intensify plant production, and there still remains inadequate scientific knowledge on their plant-related effects. This paper therefore compares the effects of two nano-fertilisers on common sunflower production under field conditions. The benefits arising from the foliar application of micronutrient-based zinc oxide fertiliser were compared with those from the titanium dioxide plant-growth enhancer. Both the zinc oxide (ZnO) and titanium dioxide (TiO₂) were delivered by foliar application in nano-size at a concentration of 2.6 mg·L⁻¹. The foliar-applied nanoparticles (NPs) had good crystallinity and a mean size distribution under 30 nm. …

Storm Surges In The Bohai Sea: The Role Of Waves And Tides, Yuanyi Li, Huan Feng, Guillaume Vigouroux, Dekui Yuan, Guangyu Zhang, Xiaodi Ma, Kun Lei

Department of Earth and Environmental Studies Faculty Scholarship and Creative Works

A storm surge is a complex phenomenon in which waves, tide and current interact. Even though wind is the predominant force driving the surge, waves and tidal phase are also important factors that influence the mass and momentum transport during the surge. Devastating storm surges often occur in the Bohai Sea, a semi-enclosed shallow sea in North China, due to extreme storms. However, the effects of waves on storm surges in the Bohai Sea have not been quantified and the mechanisms responsible for the higher surges that affect part of the Bohai Sea have not been thoroughly studied. In this …

Sediment Outflow Under Simulated Rainfall Conditions With Varying Geotechnical Properties, Pranjay Joshi, Akhilesh Kumar, P. V. Singh, Jahangeer Jahangeer

Daugherty Water for Food Global Institute: Faculty Publications

The vulnerability of soil toward erosion might be reduced by having a good vegetative cover over the soil surface, slope improvement, and improving soil properties so that it is not easily detached and transported. However, the establishment of proper vegetative cover is a long process because it takes time for seeds to germinate and attain maturity. As an alternative approach, if soil resistance was increased by increasing the shear strength of soil against erosive forces offered by eroding agents, the soil system would become capable of withstanding the detachment of its particles on the application of shear stress. To achieve …

Water Scarcity And Fish Imperilment Driven By Beef Production, Brian D. Richter, Dominique Bartak, Peter Caldwell, Kyle Fankel Davis, Peter Debaere, Arjen Y. Hoekstra, Tianshu Li, Landon Marston, Ryan Mcmanamay, Mesfin Mekonnen, Benjamin L. Ruddell, Richard R. Rushforth, Tara J. Troy

Daugherty Water for Food Global Institute: Faculty Publications

Human consumption of freshwater is now approaching or surpassing the rate at which water sources are being naturally replenished in many regions, creating water shortage risks for people and ecosystems. Here we assess the impact of human water uses and their connection to water scarcity and ecological damage across the United States, identify primary causes of river dewatering and explore ways to ameliorate them. We find irrigation of cattle-feed crops to be the greatest consumer of river water in the western United States, implicating beef and dairy consumption as the leading driver of water shortages and fish imperilment in the …

Assessment Of An Automated Calibration Of The Sebal Algorithm To Estimate Dry-Season Surface-Energy Partitioning In A Forest–Savanna Transition In Brazil, Leonardo Laipelt, Anderson Luis Ruhoff, Ayan Santos Fleischmann, Rafael Henrique Bloedow Kayser, Elisa De Mello Kich, Humberto Ribeiro Da Rocha, Christopher Michael Usher Neale

Daugherty Water for Food Global Institute: Faculty Publications

Evapotranspiration (ET) provides a strong connection between surface energy and hydrological cycles. Advancements in remote sensing techniques have increased our understanding of energy and terrestrial water balances as well as the interaction between surface and atmosphere over large areas. In this study, we computed surface energy fluxes using the Surface Energy Balance Algorithm for Land (SEBAL) algorithm and a simplified adaptation of the CIMEC (Calibration using Inverse Modeling at Extreme Conditions) process for automated endmember selection. Our main purpose was to assess and compare the accuracy of the automated calibration of the SEBAL algorithm using two different sources of meteorological …

Risk And Cost Assessment Of Nitrate Contamination In Domestic Wells, Pongpun Juntakut, Erin M.K. Haacker, Daniel D. Snow, Chittaranjan Ray

Daugherty Water for Food Global Institute: Faculty Publications

This study combines empirical predictive and economics models to estimate the cost of remediation for domestic wells exceeding suggested treatment thresholds for nitrates. A multiple logistic regression model predicted the probability of well contamination by nitrate, and a life cycle costing methodology was used to estimate costs of nitrate contamination in groundwater in two areas of Nebraska. In south-central Nebraska, 37% of wells were estimated to be at risk of exceeding a threshold of 7.5 mg/L as N, and 17% were at risk of exceeding 10 mg/L as N, the legal limit for human consumption in the United States. In …

Statistical Analysis Of Nutrient Loads From The Mississippi-Atchafalaya River Basin (Marb) To The Gulf Of Mexico, Phyllis Okwan, Yi Zhen, Huan Feng, Shinjae Yoo, Murty S. Kambhampati, Abreione Walker, Shayne Boykin, Joe Omojola, Noel Blackburn

Department of Earth and Environmental Studies Faculty Scholarship and Creative Works

This study investigated the annual and seasonal variations in nutrient loads (NO₂⁻ + NO₃⁻ and orthophosphate) delivered to the Gulf of Mexico from the Mississippi-Atchafalaya River Basin (MARB) and examined the water quality variations. The results indicate that (1) annually, the mean NO₂⁻ + NO₃⁻ and orthophosphate loads showed a steady increase during 1996–1999, a persistent level during 2000–2007, and a moderate increase during 2008–2016; (2) seasonally, NO₂⁻ + NO₃⁻ and orthophosphate in MARB in spring and summer were higher than those in autumn and winter. Analysis of …

Arjen Y. Hoekstra 1967–2019, Davy Vanham, Mesfin Mekonnen, Ashok K. Chapagain

Daugherty Water for Food Global Institute: Faculty Publications

Arjen Hoekstra introduced the water footprint in 20021, building on the concept of virtual water Tony Allan to discuss the role of trade in alleviating water scarcity in the Middle East. He thereby opened a new dimension in the debate around fair and sustainable allocation of freshwater resources. He laid the foundations to show the role of indirect water (that is, water used elsewhere to produce goods we consume) in our daily life beyond our direct use for drinking, cooking or washing. The water footprint is an indicator of direct and indirect water use by a producer or consumer, showing …

Effects Of Surface Heterogeneity Due To Drip Irrigation On Scintillometer Estimates Of Sensible, Latent Heat Fluxes And Evapotranspiration Over Vineyards, Hatim M. E. Geli, José González-Piqueras, Christopher M. U. Neale, Claudio Balbontín, Isidro Campos, Alfonso Calera

Daugherty Water for Food Global Institute: Faculty Publications

Accurate estimates of sensible (H) and latent (LE) heat fluxes and actual evapotranspiration (ET) are required for monitoring vegetation growth and improved agricultural water management. A large aperture scintillometer (LAS) was used to provide these estimates with the objective of quantifying the effects of surface heterogeneity due to soil moisture and vegetation growth variability. The study was conducted over drip-irrigated vineyards located in a semi-arid region in Albacete, Spain during summer 2007. Surface heterogeneity was characterized by integrating eddy covariance (EC) observations of H, LE and ET; land surface temperature (LST) and normalized difference vegetation index (NDVI) data from Landsat …

Assessment Of An Automated Calibration Of The Sebal Algorithm To Estimate Dry-Season Surface-Energy Partitioning In A Forest–Savanna Transition In Brazil, Leonardo Laipelt, Anderson Luis Ruhoff, Ayan Santos Fleischmann, Rafael Henrique Bloedow Kayser, Elisa De Mello Kich, Humberto Ribeiro Da Rocha, Christopher Michael Usher Neale

Daugherty Water for Food Global Institute: Faculty Publications

Evapotranspiration (ET) provides a strong connection between surface energy and hydrological cycles. Advancements in remote sensing techniques have increased our understanding of energy and terrestrial water balances as well as the interaction between surface and atmosphere over large areas. In this study, we computed surface energy fluxes using the Surface Energy Balance Algorithm for Land (SEBAL) algorithm and a simplified adaptation of the CIMEC (Calibration using Inverse Modeling at Extreme Conditions) process for automated endmember selection. Our main purpose was to assess and compare the accuracy of the automated calibration of the SEBAL algorithm using two different sources of meteorological …

Treenuts And Groundnuts In The Eat-Lancet Reference Diet: Concerns Regarding Sustainable Water Use, Davy Vanhama, Mesfin Mekonnen, Arjen Y. Hoekstra

Daugherty Water for Food Global Institute: Faculty Publications

The EAT-Lancet universal healthy reference diet recommends an increase in the consumption of healthy foods, among which treenuts and groundnuts. Both are, however, water-intensive products, with a large water footprint (WF) per unit of mass and protein and already today contribute to blue water stress in different parts of the world. The envisaged massive required increase in nut production to feed a global population with this reference diet, needs to occur in a water-sustainable way. In this paper, we identify and quantify where current nut production contributes to local blue water stress and discuss options for water-sustainable nut production. We …

Water Productivity Benchmarks: The Case Of Maize And Soybean In Nebraska, Mesfin Mekonnen, Arjen Y. Hoekstra, Christopher Michael Usher Neale, Chittaranjan Ray, Haishun Yang

Daugherty Water for Food Global Institute: Faculty Publications

Water productivity benchmarks for irrigated and rainfed agriculture will provide relevant information to manage scarce water resources and control groundwater level decline. We analyze the temporal and spatial variation of the water productivity (WP) of maize and soybean in Nebraska, with WP defined as harvested crop weight per total evapotranspiration. The results show that WP of both maize and soybean increase from west to east within Nebraska and have increased over the last 25 years, mainly due to the increase in crop yields (land productivity). We derive WP benchmarks for each crop per climate zone. Increasing actual WPs in the …

Potential Of Using Spectral Vegetation Indices For Corn Green Biomass Estimation Based On Their Relationship With The Photosynthetic Vegetation Sub-Pixel Fraction, Luan Peroni Venancio, Everardo Chartuni Mantovani, Cibele Hummel Do Amaral, Christopher M.U. Neale, Ivo Zution Gonçalves, Roberto Filgueiras, Fernando Coelho Eugenio

Daugherty Water for Food Global Institute: Faculty Publications

Crop biomass (Bio) is one of the most important parameters of a crop, and knowledge of it before harvest is essential to help farmers in their decision making. Both green and dry Bio can be estimated from vegetation spectral indices (VIs) because they have a close relationship with accumulated absorbed photosynthetically active radiation (APAR), which is proportional to total Bio. The aims of this study were to analyze the potential capacity of spectral vegetation indices in estimating corn green biomass based on their relationship with the photosynthetic vegetation sub-pixel fraction derived from spectral mixture analysis and to analyze the best …

Ferrihydrite Reduction Increases Arsenic And Uranium Bioavailability In Unsaturated Soil, Arindam Malakar, Michael Kaiser, Daniel D. Snow, Harkamal Walia, Banajarani Panda, Chittaranjan Ray

Daugherty Water for Food Global Institute: Faculty Publications

Redox driven mobilization and plant uptake of contaminants under transiently saturated soil conditions need to be clarified to ensure food and water quality across different irrigation systems. We postulate that solid-phase iron reduction in anoxic microsites present in the rhizosphere of unsaturated soil is a key driver for mobilization and bioavailability of contaminants under nonflooded irrigation. To clarify this, two major crops, corn and soybean differing in iron uptake strategies, were grown in irrigated synthetic soil under semiarid conditions with gravimetric moisture content ∼12.5 ± 2.4%. 2-line ferrihydrite, which was coprecipitated with uranium and arsenic, served as the only iron …

Use Of Multiple Environment Variety Trials Data To Simulate Maize Yields In The Ogallala Aquifer Region: A Two Model Approach, Vaishali Sharda, Mesfin Mekonnen, Chittaranjan Ray, Prasanna H. Gowda

Daugherty Water for Food Global Institute: Faculty Publications

With a long-term goal to optimize use of groundwater in the Ogallala Aquifer Region (OAR) to sustain food production systems, this study was conducted to calibrate Decision Support System for Agrotechnology Transfer (DSSAT) and AquaCrop crop modeling platforms to simulate maize production at a regional scale using historic datasets. Calibration of the models with local crop growth data and crop management practices is important, but usually this in-season crop growth information is not available. This study determined the possibility of using maize variety trial data for the evaluation of the CSM-Crop Estimation through Resources and Environmental Synthesis-Maize and AquaCrop models …

2020 Sponsorship Opportunities. Lincoln, Neb., Usa, Oct. 7-9, 2020

Daugherty Water for Food Global Institute: Administrative Materials

The Water for Food Global Conference convenes leading international experts and organizations to focus on achieving global water and food security. “Cultivating Innovation: The Next Decade,” will include keynote speakers, sessions, interactive learning and networking opportunities. The conference also celebrates the 10 year anniversary of the Daugherty Water for Food Global Institute, headlining a week of water and food security-related activities.

Topics include: • Research and Technology Innovation • Policy/Management Tools and Best Practices • Capacity Development/Youth and Students • Water Quality and Public Health • Climate Change and Ecosystems • Women for Water and Food • Nutrition and Health …

Green Strategic Planning Approach For International Shipping Activities, Xiaofang Wu, Luoping Zhang, Huan Feng

Department of Earth and Environmental Studies Faculty Scholarship and Creative Works

Sustainability is a long-term and ultimate goal for international shipping, although it is slowly making progress. The shipping perspective that moves away from “port-to-port” operations to “door-to-door” services also requires international shipping to take a long-term and holistic view instead of fragmented efforts. How to achieve the long-term sustainability goal becomes a key issue for door-to-door international shipping. Hence, green strategic planning for door-to-door international shipping was proposed with green development that puts forward the eco-centric point of view as its basic theory for sustainability. This study used a strategic decision-making approach, a so-called multi-dimensional decision-making (MDDM), coupled with the …

Small Ruminant Health Intervention Calendar In Ethiopia, Mesfin Mekonnen, Ayalew Assefa, Tesfalem Nane, Firdawok Ayele, Asrat Arke, Belay Elias, Barbara Wieland

Daugherty Water for Food Global Institute: Faculty Publications

Contents

1 Background................................................................................................... 3
2 Developing the health intervention calendar............................................................ 4
The treatment calendar ......................................................... 4 Applying the calendar..................................................................... 5 4 References................................................................... 8

Vegetation Classification And Mapping, Missouri National Recreational River, Tom Baldvins, Matt Ley, David Jones, Joe Stevens, Hannah Pilkington

United States National Park Service: Publications

Executive Summary

The vegetation inventory project at Missouri National Recreational River (MNRR) classified and mapped vegetation within the park administrative boundary and estimated thematic map accuracy quantitatively. The project was conducted over a four year period from the summer of 2015 to the winter of 2019.

The project follows guidance provided by the National Park Service (NPS) Vegetation Mapping Inventory (VMI) Program. The overall process includes initial planning and scoping, imagery procurement, field data collection, data analysis, imagery interpretation and classification, and accuracy assessment. The initial planning and scoping meetings to support study plan development took place in December, 2009, …

Effect Of Foliar Spray Application Of Zinc Oxide Nanoparticles On Quantitative, Nutritional, And Physiological Parameters Of Foxtail Millet (Setaria Italica L.) Under Field Conditions, Marek Kolenčík, Dávid Ernst, Matej Komár, Martin Šebesta, Martin Urík, Edmund Dobročka, Ivan Černý, Ramakanth Illa, Raghavendra Kanike, Qian Yu, Huan Feng, Denisa Orlová, Gabriela Kratošová

Department of Earth and Environmental Studies Faculty Scholarship and Creative Works

It has been shown that the foliar application of inorganic nano-materials on cereal plants during their growth cycle enhances the rate of plant productivity by providing a micro-nutrient source. We therefore studied the effects of foliarly applied ZnO nanoparticles (ZnO NPs) on Setaria italica L. foxtail millet’s quantitative, nutritional, and physiological parameters. Scanning electron microscopy showed that the ZnO NPs have an average particle size under 20 nm and dominant spherically shaped morphology. Energy dispersive X-ray spectrometry then confirmed ZnO NP homogeneity, and X-ray diffraction verified their high crystalline and wurtzite-structure symmetry. Although plant height, thousand grain weight, and grain …

Student Support Program Outputs, Outcomes And Impacts Report

Daugherty Water for Food Global Institute: Administrative Materials

The Robert B. Daugherty Water for Food Global Institute (DWFI) inititated its Postdoctoral and Student Support Programs in 2014. The following details their achievements.

Round One -- The institute first provided undergraduate, graduate student and postdoctoral support to faculty who were selected following a call for proposals in 2014. Support was awarded for two postdocs, five graduate students, and two projects with undergraduate students. By FY19 a small amount of support continues for Francisco Munoz-Arriola’s program. Outputs include presentations, grants and publications. The other faculty who have received support are: Vijendra Boken, UNK Geography & Earth Science; Carrick Detweiler, UNL …

Numerical Simulation Based Targeting Of The Magushan Skarn Cu-Mo Deposit, Middle-Lower Yangtze Metallogenic Belt, China, Xunyu Hu, Xiaohui Li, Feng Yuan, Alison Ord, Simon M. Jowitt, Yue Li, Wenqiang Dai, Rui Ye, Taofa Zhou

Geoscience Faculty Research

The Magushan Cu–Mo deposit is a skarn deposit within the Nanling–Xuancheng mining district of the Middle-Lower Yangtze River Metallogenic Belt (MLYRMB), China. This study presents the results of a new numerical simulation that models the ore-forming processes that generated the Magushan deposit and enables the identification of unexplored areas that have significant exploration potential under areas covered by thick sedimentary sequences that cannot be easily explored using traditional methods. This study outlines the practical value of numerical simulation in determining the processes that operate during mineral deposit formation and how this knowledge can be used to enhance exploration targeting in …

A Nanoscale Investigation Of Carlin-Type Gold Deposits: An Atom-Scale Elemental And Isotopic Perspective, Philippe Gopon, James O. Douglas, Maria A. Auger, Lars Hansen, Jon Wade, Jean S. Cline, Laurence J. Robb, Michael P. Moody

Geoscience Faculty Research

Carlin-type gold deposits are one of the most important gold mineralization styles in the world. Despite their economic importance and the large volume of work that has been published, there remain crucial questions regarding their metallogenesis. Much of this uncertainty is due to the cryptic nature of the gold occurrence, with gold occurring as dispersed nanoscale inclusions within host pyrite rims that formed on earlier formed barren pyrite cores. The small size of the gold inclusions has made determining their nature within the host sulfides and the mechanisms by which they precipitated from the ore fluids particularly problematic. This study …