Environmental Health and Protection Commons^™

Tracing Nutrient Inputs Into A Eutrophic Lake Using Nitrogen Isotopes, Wilgreen Lake, Madison County, Kentucky, Walter S. Borowski, Theresa A. Aguiar

EKU Faculty and Staff Scholarship

Wilgreen Lake is a dammed lake that has been classified as nutrient-impaired (303d list) by the EPA and State of Kentucky. The lake is moderately-sized covering 169 acres (0.7 km²), and drains a watershed with residential developments, cattle pasture, modified woodlands, and some industrial/urban usage in the city of Richmond. The principal tributaries are Taylor Fork and Old Town Branch that meet to form the trunk of the lake approximately one mile in length. The upper reaches of Taylor Fork are adjacent to a densely-packed (quarter-acre lots) housing development with septic systems. Old Town Branch drains cattle pasture …

Using E. Coli And Bacteroides Distribution And Abundance In A Eutrophic Lake As A Tracer For Nutrient Inputs, Wilgreen Lake, Madison County, Kentucky, Theresa A. Aguiar, Walter S. Borowski, Alice C. Layton, Larry Mckay

EKU Faculty and Staff Scholarship

Wilgreen Lake is a eutrophic lake that has been listed on the EPA’s 303d list as nutrient impaired. Potential sources of this impairment are likely from humans, cattle manure, and fertilizers. We suspect that the majority of nutrients originate from human sources, namely from septic tank effluent emanating from key housing developments ringing the lakeshore. We test our hypothesis with conventional microbial assays (Escherichia coli) and RT-PCR techniques (Bacteroides).

We took water samples at 19 sampling locations on 4 occasions, and measured the abundance of Escherichia coli using IDEXX methods. Corresponding sub-samples slated for potential PCR …

Fecal Microbe Distribution And Abundance Used As A Possible Proxy For Nutrient Source Identification In Eutrophic Wilgreen Lake, Madison County, Kentucky, Theresa A. Aguiar, Walter S. Borowski

EKU Faculty and Staff Scholarship

Wilgreen Lake is a eutrophic lake that has been listed on the EPA’s 303d list as nutrient impaired. Potential sources of this impairment are from humans, cattle manure and fertilizers. We suspect that the majority of nutrients originate from human sources. As a possible proxy for nutrient input, we test our hypothesis by examining fecal microbe distribution and abundance in the lake.

We took water samples at 19 sampling locations on 4 occasions. Sampling spanned 26 June to 15 August with the last 3 sampling events occurring at roughly two-week intervals. These samples were then processed using IDEXX methods, which …

Preliminary Results Of A Fecal Microbe Survey In An Eutrophic Lake, Wilgreen Lake, Madison County, Kentucky, Walter S. Borowski, Michael S. Albright

EKU Faculty and Staff Scholarship

Wilgreen Lake is a small (~14 mi²) eutrophic lake formed by damming several tributary streams to Silver Creek, Madison County, Kentucky. The lake receives runoff from industrial and urban areas (Richmond) that comprise ~10% of the total watershed area; most runoff is from cattle pasture or human developments encircling the lake. Present and past developments are on septic systems, and effluent from these systems is known qualitatively to seep into lake waters.

Our research group is currently conducting a study of the lake in order to identify major nutrient sources, and one possible tracer method is to quantitatively …

Preliminary Results Of A Nutrient Source Study In Wilgreen Lake, Madison County, Kentucky, Walter S. Borowski, Erin C. Jolly

EKU Faculty and Staff Scholarship

Wilgreen Lake (Madison County, Kentucky) is an eutrophic lake formed by damming Taylor Fork, part of the Silver Creek watershed. Two principal tributaries drain urban areas of the city of Richmond, agricultural land typified by cattle grazing, and a high-density residential area using septic systems. The lake is “nutrient impaired,” so it is likely that anthropogenic nutrient loading is affecting water quality. Our study aims to first characterize the physical characteristics and water quality of the lake (2006), and then determine the specific proportion of nutrient inputs (2007) to the lake with the aim of remediating any possible degradation of …