Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 2 of 2
Full-Text Articles in Other Chemistry
Two-Stage Mechanism For Activation Of The Dna Replication Checkpoint Kinase Cds1 In Fission Yeast, Xu Yong, Matthew Davenport, Thomas Kelly
Two-Stage Mechanism For Activation Of The Dna Replication Checkpoint Kinase Cds1 In Fission Yeast, Xu Yong, Matthew Davenport, Thomas Kelly
Matthew P Davenport
The DNA replication checkpoint is a complex signal transduction pathway, present in all eukaryotic cells, that functions to maintain genomic integrity and cell viability when DNA replication is perturbed. In Schizosaccharomyces pombe the major effector of the replication checkpoint is the protein kinase Cds1. Activation of Cds1 is known to require the upstream kinase Rad3 and the mediator Mrc1, but the biochemical mechanism of activation is not well understood. We report that the replication checkpoint is activated in two stages. In the first stage, Mrc1 recruits Cds1 to stalled replication forks by interactions between the FHA domain of Cds1 and …
Extraction And Characterization Of Lipids From Antarctic Krill (< I> Euphausia Superba), Joseph Gigliotti, Matthew Davenport, Sarah Beamer, Janet Tou, J Jaczynski
Extraction And Characterization Of Lipids From Antarctic Krill (< I> Euphausia Superba), Joseph Gigliotti, Matthew Davenport, Sarah Beamer, Janet Tou, J Jaczynski
Matthew P Davenport
There is significant commercial interest in oil extraction from krill because it is rich in omega-3 polyunsaturated fatty acids (n-3 PUFA) such as eicosapentaenoic (EPA, 20:5n3) and docosahexaenoic (DHA, 22:6n3) acids. The objectives were to determine oil extraction efficiency using different solvent systems and the composition of extracted oil and spent krill following extraction. Extraction efficiency was the highest (P < 0.05) for one-step extraction using freeze-dried krill with 1:12 or 1:30 krill:solvent ratio (w:v) compared to Folch, Soxhlet, and conventional two-step extraction. Extracted oils contained predominantly phospholipids (20–33%), polar non-phospholipids (64–77%), and minor triglycerides (1–3%). Triglycerides contained much less (P …