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Development Of Microsatellite Markers In Cannabis Sativa For Fingerprinting And Genetic Relatedness Analyses, Hussain J. Al-Ghanim
Development Of Microsatellite Markers In Cannabis Sativa For Fingerprinting And Genetic Relatedness Analyses, Hussain J. Al-Ghanim
FIU Electronic Theses and Dissertations
Microsatellite markers were developed for Cannabis sativa L. (marijuana) to estimate the level of polymorphism, usefulness for DNA typing (genotype identification), and to measure the genetic relationships between the different plants. Twelve different oligonucleotide probes were used to screen an enriched microsatellite library of Cannabis sativa in which 49% of the clones contained microsatellite sequences. Characterization of microsatellite loci in Cannabis revealed that GA/CT was the most abundant class of isolated microsatellites representing 50% overall. Eleven polymorphic SSR markers were developed, derived from dinucleotide motifs and eight from trinucleotide motifs. A total of 52 alleles were detected averaging 4.7 alleles/locus. …