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Full-Text Articles in Physical Sciences and Mathematics
Cucurbit[7]Uril-Tetramethylrhodamine Conjugate For Direct Sensing And Cellular Imaging, Andrew T. Bockus, Lauren C. Smith, Amy G. Grice, Omar A. Ali, Carolyn C. Young, William Mobley, Ashley Leek, James Lewis Roberts, Brittany Vinciguerra, Lyle D. Isaacs, Adam R. Urbach
Cucurbit[7]Uril-Tetramethylrhodamine Conjugate For Direct Sensing And Cellular Imaging, Andrew T. Bockus, Lauren C. Smith, Amy G. Grice, Omar A. Ali, Carolyn C. Young, William Mobley, Ashley Leek, James Lewis Roberts, Brittany Vinciguerra, Lyle D. Isaacs, Adam R. Urbach
Chemistry Faculty Research
This paper describes the design and synthesis of a conjugate (Q7R) comprising the synthetic host cucurbit[7]uril (Q7) linked to the fluorescent dye tetramethylrhodamine (TMR), and the characterization of its optical and guest-binding properties as well as its cellular uptake. Q7R was synthesized in two steps from monofunctionalized azidobutyl-Q7 and NHS-activated TMR. The fluorescence of Q7R is quenched upon guest binding, and this observable was used to determine equilibrium dissociation constant (Kd) values. Unexpectedly, the Kd values for guests binding to Q7R and to unmodified Q7 were essentially identical. Therefore, Q7R can directly report binding to Q7 without …
Polyglutamine Aggregates Impair Lipid Membrane Integrity And Enhance Lipid Membrane Rigidity, Chian Sing Ho, Nawal K. Khadka, Fengyu She, Jianfeng Cai, Jianjun Pan
Polyglutamine Aggregates Impair Lipid Membrane Integrity And Enhance Lipid Membrane Rigidity, Chian Sing Ho, Nawal K. Khadka, Fengyu She, Jianfeng Cai, Jianjun Pan
Physics Faculty Publications
Lipid membranes are suggested as the primary target of amyloid aggregates. We study aggregates formed by a polyglutamine (polyQ) peptide, and their disruptive effect on lipid membranes. Using solution atomic force microscopy (AFM), we observe polyQ oligomers coexisting with short fibrils, which have a twisted morphology that likely corresponds to two intertwined oligomer strings. Fourier transform infrared spectroscopy reveals that the content of β-sheet enriched aggregates increases with incubation time. Using fluorescence microscopy, we find that exposure to polyQ aggregates results in deflated morphology of giant unilamellar vesicles. PolyQ aggregates induced membrane disruption is further substantiated by time-dependent calcein leakage …