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Full-Text Articles in Physical Sciences and Mathematics
Investigation Of Photophysical And Photochemical Processes In Conjugated Polymer Nanoparticles By Single Particle And Ensemble Spectroscopy, Craig Szymanski
Investigation Of Photophysical And Photochemical Processes In Conjugated Polymer Nanoparticles By Single Particle And Ensemble Spectroscopy, Craig Szymanski
All Dissertations
Single molecule imaging has emerged as a powerful tool in a range of applications, but the field is limited by a lack of fluorescent probes with sufficient brightness and photostability for many demanding applications such as tracking of single biomolecules in cells and tissues at video rate with 1 nm spatial resolution. Conjugated polymers hold great promise as a solution to these issues, with a high density of π electrons and variety of chemistries, allowing efficient and tunable absorption of light. This dissertation describes the development and characterization of a novel type of nanoparticle composed of conjugated polymer called CPdots. …
Fluorescent Conjugated Polymer Dots For Single Molecule Imaging And Sensing Applications, Changfeng Wu
Fluorescent Conjugated Polymer Dots For Single Molecule Imaging And Sensing Applications, Changfeng Wu
All Dissertations
While single molecule imaging and sensing hold the promise of providing unprecedented detail about cellular processes, many advanced applications are limited by the lack of appropriate fluorescence probes. In many cases, currently available fluorescent labels are not sufficiently bright and photostable to overcome the background associated with various autofluorescence and scattering processes. This dissertation describes research efforts focused on the development of a novel class of fluorescent nanoparticles called conjugated polymers dots (CPdots) for single molecule fluorescence detection. The CPdots contain highly fluorescent π-conjugated polymers that have been refined over the last decades as the active material in polymer light-emitting …