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Cell Toxicity And Uptake Of Rrr-Alpha-Tocopheryl Polyethylene Glycol 1000 Succinate (Tpgs) By Various Cell Ines In Vitro., Christelle Komguem Kamga
Cell Toxicity And Uptake Of Rrr-Alpha-Tocopheryl Polyethylene Glycol 1000 Succinate (Tpgs) By Various Cell Ines In Vitro., Christelle Komguem Kamga
Electronic Theses and Dissertations
This research focused on investigating and comparing the cytotoxicity and cellular uptake of RRR-alpha-tocopheryl polyethylene glycol succinate (TPGS, with that of alpha-tocopheryl succinate (α-TS). Both TPGS and α-TS are water-soluble forms of vitamin E with important clinical applications. Cytotoxicity assays with RAW 264.7 and LNCaP cells incubated overnight with TPGS or α-TS at concentrations ≥ 12.4 μM suggest that α-TS is more cytotoxic than TPGS. Macrophages were found to be more sensitive than LNCaP cells when treated with similar concentrations of α-TS. For both cell lines, most of the TPGS or α-TS taken up remained esterified after 24 hours. Our …
Cell Toxicology Study Of Rrr-Alpha-Tocopheryl Polyethylene Glycol 1000 Succinate (Tpgs)., Clarisse Sornsay Muenyi
Cell Toxicology Study Of Rrr-Alpha-Tocopheryl Polyethylene Glycol 1000 Succinate (Tpgs)., Clarisse Sornsay Muenyi
Electronic Theses and Dissertations
This research focused on the cytotoxic properties of RRR-alpha-tocopheryl polyethylene glycol 1000 succinate (TPGS) in transformed and cancerous cell lines. We used RAW264.7 macrophage and prostate cancer (LNCaP) cell lines in this study. TPGS caused cell death and decreased cell viability in a dose and time dependent manner. Cell death was evaluated fluorimetrically by employing the nucleic acid-binding fluorophore; propidium iodide. A colorimetric 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay was used to evaluate cell viability. Cell death can occur through necrosis or apoptosis. Our results suggested that TPGS triggered apoptotic cell death. Induction of apoptosis, as measured by caspase …