Open Access. Powered by Scholars. Published by Universities.®
Physical Sciences and Mathematics Commons™
Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 3 of 3
Full-Text Articles in Physical Sciences and Mathematics
Cell Toxicity And Uptake Of Rrr-Alpha-Tocopheryl Polyethylene Glycol 1000 Succinate (Tpgs) By Various Cell Ines In Vitro., Christelle Komguem Kamga
Cell Toxicity And Uptake Of Rrr-Alpha-Tocopheryl Polyethylene Glycol 1000 Succinate (Tpgs) By Various Cell Ines In Vitro., Christelle Komguem Kamga
Electronic Theses and Dissertations
This research focused on investigating and comparing the cytotoxicity and cellular uptake of RRR-alpha-tocopheryl polyethylene glycol succinate (TPGS, with that of alpha-tocopheryl succinate (α-TS). Both TPGS and α-TS are water-soluble forms of vitamin E with important clinical applications. Cytotoxicity assays with RAW 264.7 and LNCaP cells incubated overnight with TPGS or α-TS at concentrations ≥ 12.4 μM suggest that α-TS is more cytotoxic than TPGS. Macrophages were found to be more sensitive than LNCaP cells when treated with similar concentrations of α-TS. For both cell lines, most of the TPGS or α-TS taken up remained esterified after 24 hours. Our …
Cell Toxicology Study Of Rrr-Alpha-Tocopheryl Polyethylene Glycol 1000 Succinate (Tpgs)., Clarisse Sornsay Muenyi
Cell Toxicology Study Of Rrr-Alpha-Tocopheryl Polyethylene Glycol 1000 Succinate (Tpgs)., Clarisse Sornsay Muenyi
Electronic Theses and Dissertations
This research focused on the cytotoxic properties of RRR-alpha-tocopheryl polyethylene glycol 1000 succinate (TPGS) in transformed and cancerous cell lines. We used RAW264.7 macrophage and prostate cancer (LNCaP) cell lines in this study. TPGS caused cell death and decreased cell viability in a dose and time dependent manner. Cell death was evaluated fluorimetrically by employing the nucleic acid-binding fluorophore; propidium iodide. A colorimetric 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay was used to evaluate cell viability. Cell death can occur through necrosis or apoptosis. Our results suggested that TPGS triggered apoptotic cell death. Induction of apoptosis, as measured by caspase …
Structural Study Of Disease Related Proteins, Ayse Sinem Ozyurt
Structural Study Of Disease Related Proteins, Ayse Sinem Ozyurt
Electronic Theses and Dissertations
The availability of complete sequences for the genomes of many organisms changes the researcher's attention from genome sequencing to finding the function of gene products, the proteins. The three dimensional (3D) structure of a protein is helpful to understand its function, because tertiary structure is more conserved in evolution than sequence. The 3D structures can be found quickly by using high-throughput (HT) methods for protein expression, purification, characterization and structure determination. This process is very challenging in every step. Therefore we selected nearly 200 cancer related proteins as targets to see the difficulties in trying to determine the web predicted …