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Engineering Sortase; Activity And Selectivity Of New Hybrid And Ancestral Variants Of Sortase A, Sarah Struyvenberg
Engineering Sortase; Activity And Selectivity Of New Hybrid And Ancestral Variants Of Sortase A, Sarah Struyvenberg
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Bacterial sortase enzymes are a beneficial tool in innovative mechanisms of protein engineering. However, important limitations to utilization of sortases for engineered purposes exist; namely, that sortase A (SrtA) is a relatively poor enzyme and very specific for the substrate containing LPATXG motif. Exciting previous work from our collaborators reveals that sortases from different species recognize different sequences and that activity can vary. Therefore, we wanted to create and investigate hybrid sortase enzymes between SrtA from S. aureus and Streptococcus pneumoniae, wherein we swapped a substrate-interacting loop between the beta-E and beta-F strands. Our hypothesis is that these residues are …