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Full-Text Articles in Physical Sciences and Mathematics
Engineering Sortase; Activity And Selectivity Of New Hybrid And Ancestral Variants Of Sortase A, Sarah Struyvenberg
Engineering Sortase; Activity And Selectivity Of New Hybrid And Ancestral Variants Of Sortase A, Sarah Struyvenberg
Scholars Week
Bacterial sortase enzymes are a beneficial tool in innovative mechanisms of protein engineering. However, important limitations to utilization of sortases for engineered purposes exist; namely, that sortase A (SrtA) is a relatively poor enzyme and very specific for the substrate containing LPATXG motif. Exciting previous work from our collaborators reveals that sortases from different species recognize different sequences and that activity can vary. Therefore, we wanted to create and investigate hybrid sortase enzymes between SrtA from S. aureus and Streptococcus pneumoniae, wherein we swapped a substrate-interacting loop between the beta-E and beta-F strands. Our hypothesis is that these residues are …
Progress Towards The Substrate-Bound Structure Of Streptococcus Pneumoniae Sortase A, Nicholas M. Horvath
Progress Towards The Substrate-Bound Structure Of Streptococcus Pneumoniae Sortase A, Nicholas M. Horvath
WWU Graduate School Collection
Sortases are cysteine transpeptidases found primarily on the cell surface of Gram-positive bacteria. Sortase-mediated ligations have become an attractive option for protein modification chemistry, enabling the synthesis of a wide range of non-natural polypeptide derivatives. Attempts at understanding how these enzymes recognize and bind substrates are integral to furthering their usefulness in protein engineering and, potentially, treatment of bacterial diseases. However, the variable substrate specificity and activity between homologs of these enzymes is not yet fully understood. Of specific interest to us is sortase A from Streptococcus pneumoniae (SrtApneu), as it demonstrates a broad substrate tolerance not …