Physical Sciences and Mathematics Commons^™

Probe-Level Statistical Models For Differential Expression Of Genes In Bovine Nt Studies, Jason L. Bell

All Graduate Plan B and other Reports, Spring 1920 to Spring 2023

A brief introduction of microarray technology and its uses is given. This technology is commonly used in agricultural research, including research in nuclear transfer, which motivated this study. There are 3 classes of statistical models compared: probeset-level, weighted probeset-level and probe-level.

Different statistical mod els are compared on 3 spike-in experiments to assess the relative performance in identifying differentially expressed genes . A novel nested factorial model was found to outperform all other models compared in this study in one spike-in experiment, and was found to be competitive in its performance relative to the other models on the other spike-in …