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Comparative Enzymology Of Glyoxalase I, Christina Margaret Schimandle
Comparative Enzymology Of Glyoxalase I, Christina Margaret Schimandle
Chemistry and Chemical Biology ETDs
The role of the metal ion in rat erythrocyte glyoxalase I was evaluated. The apoenzyme was formed by dialysis against an EDTA-imidazole buffer. Fluorescence studies of the Mg++- and Mn++-dansylated glyoxalase I's suggest that the metal ion is near the active site of the enzyme and participates in the catalytic reaction. Stereochemical studies of the conversion of methylglyoxal to S-lactoylglutathione with Mg++, Mn++, Co++ and Ni++-glyoxalase I's show that the 0-lactate of S-lactoylglutathione (97±1.5%) is formed. This result suggests that the reaction mechanism is the same with a change …