Physical Sciences and Mathematics Commons^™

The Biochemical Assessment Of Two Secreted Acid Phosphatases From Leishmania Tarentolae, Their Response To Electric Fields, Glycosidase Incubation, And / Or Vanadium, Benjamin M. Dorsey

Theses and Dissertations

Leishmaniasis, as defined by the Center for Disease Control and Prevention, is a neglected tropical disease with 1.6 million new cases reported each year. However, there is yet to be safe, effective, and affordable treatments provided to those affected by this disease1. Still underappreciated as a potential pharmaceutical targets, especially for cutaneous leishmaniasis infections, are the two isozymes of secreted acid phosphatase (SAP); secreted acid phosphatase 1 (SAP1) and secreted acid phosphatase 2 (SAP2). These enzymes are involved in the survival of the parasite in the sand fly vector, and the prevention of host macrophages from forming parasitophorous vacuole and …

Kinetic Characterization Of Listeria Monocytogenes 2-C- Methyl-D-Erythritol 4-Phosphate Cytidylyltransferase ( Cms ) Enzymes Using High Performance Liquid Chromatography ( Hplc ), Mark Oblazny

Theses and Dissertations

Infectious diseases, with increasing prevalence of antibiotic resistant bacteria, coupled with the declining rate in discovery of antimicrobial agents, impose one of the most significant threats to human health. Here we identify 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (CMS) as a valid target for antibiotic development which is an enzyme in pathogenic organisms that leads to the biosynthesis of isoprene precursor molecules. Isoprene molecules are one of nature’s most common building blocks that are vital to many biological metabolic processes and are synthesized via the mevalonic acid dependent (MVA), or methylerythritol phosphate (MEP) pathway. Vertebrates utilize the MVA pathway, while many pathogenic bacteria …

Diurnal And Seasonal Variation In Groundwater Nitrate -N Concentration In A Saturated Buffer Zone, Joseph Miller

Theses and Dissertations

Surface water pollution by nitrate (NO3-) is identified as a critical problem in agricultural land-use areas. Excess NO3- loading causes eutrophication and hypoxia in near-shore marine waters such as the Gulf of Mexico. Diversion of agricultural runoff into saturated buffer zones reduces NO3- loading. Although the mechanisms responsible for NO3- reduction in saturated buffer zones are well characterized, little is known about how NO3- concentrations vary temporally and what factors control those changes. The objective of this study is to understand NO3- concentration and environmental factor variability in a saturated buffer zone on a diurnal scale within and among the …

Fermenting Solutions To New World Problems: Bioremediation Of Blue Agave And Cocoa Pod Wastes, Daniel Hart

Theses and Dissertations

Waste, minimizing it, and maximizing profit from it are major foci of most agricultural production processes today. Waste from two major production processes, namely tequila production and cocoa production, share several characteristics. Both these processes are still carried out as they have been for hundreds of years. They are both relatively concentrated in location; all tequila is produced in a small region of Mexico while cocoa pods only grow in tropical environments within 18° of the equator. In addition, both processes used to produce these commodities remain fairly inefficient; they generate huge amounts of waste that goes mostly unused. With …

Modeling The Influence Of El Niño On Parasite Transmission In Sand Crab Populations And Seabird Abundance Along The Californian Coast, Aboubacar Dio Seck

Theses and Dissertations

Pacific mole crabs (Emerita analoga) are one of the most important and abundant

invertebrates in sandy beach environments. Consequently, they are a common food source

for seabirds and sea otters. Since the mole crab serves as the primary intermediate host for

acanthocephalan parasites, they have been linked to a number of mortality events. It is

currently estimated that 13-16% of deaths in the threatened California sea otter population

have been caused by infection. In addition, unusually high loads of acanthocephalan

parasites have been linked to episodic deaths of thousands of surf scoters. Studies suggest

that acanthocephalan development and transmission may …

Evaluating Relationships Between Rock Strength And Longitudinal Stream Profile Morphometry In The Southern Guadalupe Mountains, Texas, Samuel T. Schoenmann

Theses and Dissertations

Landscapes record information about the tectonic, climatic, and lithologic environments in which they form (Yang et al., 2015). When one or more of these environmental conditions change spatially or temporally, the landscape responds through erosion and thus, develops representative geomorphic features (Ritter et al., 2011). Since the nineteenth century, it has been clear that bedrock strength and erodibility play an important role in landscape evolution and geomorphology (Lifton et al., 2009). However, the nuances of variable erodibility remain poorly understood. The implications of this limited understanding lies within landscape evolution models. While these models show strong qualitative relationships between longitudinal …

Evaluating Fault-Line Escarpment Exposure In The Guadalupe Mountains Of West Texas And New Mexico, Audrey Anne Happel

Theses and Dissertations

The Permian stratigraphy of the Guadalupe Mountains is well-known due to the impeccable exposure of Permian strata along distinct escarpments that define the boundaries of the range. Even though the Permian strata have been closely examined to understand the associated petroleum reservoirs in the adjacent Delaware basin, little work has been done on the escarpments that expose the well-known rocks at the surface by way of tectonic and erosional processes. The development of escarpments are directly affected by multiple processes that create the landscape, and can be used as a tool to temporally and spatially constrain tectonic and erosional activity …

Development Of New Routes For The Synthesis Of Carbaporphyrins And Carbachlorins, Navneet Sahota

Theses and Dissertations

Carbaporphyrins and carbachlorins are porphyrin analogs in which one of the nitrogens is replaced with a carbon atom within a porphyrin-like cavity. These systems form stable organometallic derivatives and exhibit unusual chemical reactivity. In the monocarbaporphyrinoids such as benzocarbaporphyrins, one of the pyrrole rings is replaced by an indene unit. Although syntheses of monocarbaporphyrins with fused rings have been widely investigated over the past 20 years, a few examples of carbaporphyrins without fused rings have been reported in the literature.

In this thesis, two projects were conducted to further explore carbaporphyrin and carbachlorin chemistry. The first project utilized a ‘3+1’ …

Nutrient Loading Reduction In A Tile Drained Agricultural Watershed Through Watershed-Scale Cover Cropping: A High Resolution Analysis, Benjamin Gerald Bruening

Theses and Dissertations

Nutrient pollution originating from agricultural regions in the Midwest is a serious issue, leading to pollution of drinking water sources as well as large hypoxic zones in the Gulf of Mexico. The source of much of this contamination has been shown to be runoff from agricultural fields in the Upper Mississippi River Basin. One method that has been shown to reduce this pollution from the Upper Mississippi River Basin is the planting of winter cover crops. Winter cover crops such as rye and tillage radish have been shown to significantly reduce nitrate exported from agricultural fields, even in tile-drained watersheds …

Creating A Scaling Relationship Between Peak Discharge And Drainage Area To Identify Tile Drainage Inputs Into An Agricultural Stream, Ryan Plath

Theses and Dissertations

Tile drains have been shown to contribute to high levels of nitrate in agricultural streams. Locations of tile drains on a watershed scale, however, are often unknown due to tile drains being located on many separate parcels of private property. This study evaluates the ability of a methodology, using scaling relationships between discharge and drainage area, for locating areas of large tile drainage contribution to Money Creek, in McLean County, Illinois. Additionally, this study examines the difference in scaling relationships and physical stream hydrology between tileflow and no-tileflow conditions. Eight stream sites were created in the watershed, that recorded stage …

Exploration Of The Northern Illinois Bedrock Surface, Matthew Rhoads

Theses and Dissertations

The purpose of this study is to deconstruct the relationship between the Leaf River anticline and the preglacial bedrock paleo topography at the eastern terminus of the Plum River Fault Zone in Ogle County, Illinois. A geostatistical approach was used to model the subsurface elevation of the bedrock paleo topography and the Leaf River anticline. The contour maps derived from the elevation models provided detailed depictions of the ancient bedrock landscape and subsurface structure in the study area. The Leaf River anticline is interpreted to be a component of hanging wall anticline at the terminus of the Plum River Fault …

Dynamics Of Nitrate, Phosphorus, And Suspended Sediment Transport In Two Agricultural Streams In Central Illinois, Luke W. Lampo

Theses and Dissertations

Nutrients such as nitrate and phosphorus are necessary for life, but excessive amounts can be detrimental. Large amounts of nutrients entering bodies of water can lead to hypoxic zones such as the one in the Gulf of Mexico. Nutrients are also problematic in drinking water reservoirs, as high concentrations of nitrate in drinking water can cause health conditions such as blue baby syndrome and high phosphorus concentrations can lead to algal blooms. Suspended sediment leads to reservoir sedimentation, habitat degradation, and is able to transport particulate nutrients. High nutrient and sediment concentrations are a recurring problem in the drinking water …

Formation, Characterization, And Optimization Of Antibody-Gold Nanoparticle Conjugates, Seth L. Filbrun

Theses and Dissertations

Protein modified gold nanoparticle based immunoassays are the basis of many novel detection techniques. There are many groups working on novel immunoassays but there is still much to understand about how many proteins are attached onto each nanoparticle and much to improve on immobilization methods. This thesis work is devoted to improving techniques for the quantification of protein immobilized on the gold nanoparticle and development of a novel approach to immobilize protein independent of pH.

The ability to evaluate antibody immobilization onto gold nanoparticles is critical for assessing coupling chemistry and optimizing the sensitivity of nanoparticle-enabled biosensors. Herein, we developed …

Death And Destruction: Insight Into The Rhino Poaching Epidemic In South Africa, Richard Wayne Charlton

Theses and Dissertations

The poaching of wildlife has been a concern to many involved in the protection of wildlife. Poaching is found throughout the world and has been around for thousands of years. In the past century, poaching has reached new heights and the concern of an extinction of a species has bought the topic of poaching to new light. Whilst poaching has been seen as a biological or conservation topic in the past, criminologists around the world have now started to delve into the topic. The poaching of wildlife and more so in recent years, rhino poaching, has been a concern in …