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Extracellular Gluco-Oligosaccharide Degradation By Caulobacter Crescentus, Gerald N. Presley, Matthew J. Payea, Logan R. Hurst, Annie E. Egan, Brandon S. Martin, Gopal R. Periyannan
Extracellular Gluco-Oligosaccharide Degradation By Caulobacter Crescentus, Gerald N. Presley, Matthew J. Payea, Logan R. Hurst, Annie E. Egan, Brandon S. Martin, Gopal R. Periyannan
Gopal R. Periyannan
The oligotrophic bacterium Caulobacter crescentus has the ability to metabolize various organic molecules, including plant structural carbohydrates, as a carbon source. The nature of β-glucosidase (BGL)-mediated gluco-oligosaccharide degradation and nutrient transport across the outer membrane in C. crescentus was investigated. All gluco-oligosaccharides tested (up to celloheptose) supported growth in M2 minimal media but not cellulose or CM-cellulose. The periplasmic and outer membrane fractions showed highest BGL activity, but no significant BGL activity was observed in the cytosol or extracellular medium. Cells grown in cellobiose showed expression of specific BGLs and TonB-dependent receptors (TBDRs). Carbonyl cyanide 3-chlorophenylhydrazone lowered the rate of …