Physical Sciences and Mathematics Commons^™

The Epididymal Soluble Prion Protein Forms A High-Molecular-Mass Complex In Association With Hydrophobic Proteins, Heath W. Ecroyd, Maya Belghazi, Jean-Louis Dacheux, Jean-Luc Gatti

Heath Ecroyd

We have shown previously that a 'soluble' form of PrP (prion protein), not associated with membranous vesicles, exists in the male reproductive fluid [Ecroyd, Sarradin, Dacheux and Gatti (2004) Biol. Reprod. 71, 993-1001]. Attempts to purify this 'soluble' PrP indicated that it behaves like a high-molecular-mass complex of more than 350 kDa and always co-purified with the same set of proteins. The main associated proteins were sequenced by MS and were found to match to clusterin (apolipoprotein J), BPI (bacterial permeability-increasing protein), carboxylesterase-like urinary excreted protein (cauxin), beta-mannosidase and beta-galactosidase. Immunoblotting and enzymatic assay confirmed the presence of clusterin and …

Compartmentalization Of Prion Isoforms Within The Reproductive Tract Of The Ram, Heath W. Ecroyd, Pierre Sarradin, Jean-Louis Dacheux, Jean-Luc Gatti

Heath Ecroyd

Cellular prion protein (Prp(C)) is a glycoprotein usually associated with membranes via its glycosylphosphatidylinositol (GPI) anchor. The trans-conformational form of this protein (Prp(SC)) is the suggested agent responsible for transmissible neurodegenerative spongiform encephalopathies. This protein has been shown on sperm and in the reproductive fluids of males. Antibodies directed against the C-terminal sequence near the GPI-anchor site, an N-terminal sequence, and against the whole protein showed that the Prp isoforms were compartmentalized within the reproductive tract of the ram. Immunoblotting with the three antibodies showed that the complete protein and both N- and C-terminally truncated and glycosylated isoforms are present …

Post-Testicular Sperm Environment And Fertility, Jean-Luc Gatti, Sandrine Castella, Francoise Dacheux, Heath Ecroyd, S Metayer, Veronique Thimon, Jean-Louis Dacheux

Heath Ecroyd

When mammalian spermatozoa exit the testis, they show a highly specialized morphology; however, they are not yet able to carry out their task: to fertilize an oocyte. This property, that includes the acquisition of motility and the ability to recognize and to fuse with the oocyte investments, is gained only after a transit through the epididymis during which the spermatozoa from the testis travel to the vas deferens. The exact molecular mechanisms that turn these cells into fertile gametes still remain mysterious, but surface-modifying events occurring in response to the external media are key steps in this process. Our laboratory …

Protective Interactions Of Dairy Peptides With Fibril Structures And Relevance To Alzheimer's Disease, Louise Bennett, Williams Roderick, Heath Ecroyd, Yanqin Liu, Sunanda Sudharmarajan, John Carver

Heath Ecroyd

Selected dairy caseins have been shown to have capacity for chaperone-like regulation of folding pathways of other caseins, specifically in preventing development of fibrillar aggregates of beta sheet structure. An assay based on fibril formation by reduced and carboxymethylated-kappa casein (RCM-kCn) was thus used to screen for anti-fibril activity among a selection of dairy protein hydrolysates, in order to discover peptides with possible anti-fibril, chaperone activity. From the selection of eight dairy hydrolysates based on different dairy protein fractions, two of the hydrolysates of whey protein exhibited superior anti-fibril bioactivity against RCM-kCn and amyloid beta (Aβ), the peptide associated with …

Small Heat-Shock Proteins Interact With A Flanking Domain To Suppress Polyglutamine Aggregation, Amy L. Roberston, Stephen J. Headey, Helen M. Saunders, Heath Ecroyd, Martin J. Scanlon, John A. Carver, Stephen P. Bottomley

Heath Ecroyd

Small heat-shock proteins (sHsps) are molecular chaperones that play an important protective role against cellular protein misfolding by interacting with partially unfolded proteins on their off-folding pathway, preventing their aggregation. Polyglutamine (polyQ) repeat expansion leads to the formation of fibrillar protein aggregates and neuronal cell death in nine diseases, including Huntington disease and the spinocerebellar ataxias (SCAs). There is evidence that sHsps have a role in suppression of polyQ-induced neurodegeneration; for example, the sHsp alphaB-crystallin (αB-c) has been identified as a suppressor of SCA3 toxicity in a Drosophila model. However, the molecular mechanism for this suppression is unknown. In this …

Testicular Descent, Sperm Maturation And Capacitation. Lessons From Our Most Distant Relatives, The Monotremes, Russell Jones, Heath Ecroyd, Jean-Louis Dacheux, Brett Nixon

Heath Ecroyd

The present review examines whether monotremes may help to resolve three questions relating to sperm production in mammals: why the testes descend into a scrotum in most mammals, why spermatozoa are infertile when they leave the testes and require a period of maturation in the specific milieu provided by the epididymides, and why ejaculated spermatozoa cannot immediately fertilise an ovum until they undergo capacitation within the female reproductive tract. Comparisons of monotremes with other mammals indicate that there is a need for considerable work on monotremes. It is hypothesised that testicular descent should be related to epididymal differentiation. Spermatozoa and …

The Development Of Signal Transduction Pathways During Epididymal Maturation Is Calcium Dependent, Heath W. Ecroyd, Kelly Asquith, Russell C. Jones, Robert J. Aitken

Heath Ecroyd

Capacitation has been correlated with the activation of a cAMP-PKA-dependent signaling pathway leading to protein tyrosine phosphorylation. The ability to exhibit this response to cAMP matures during epididymal maturation in concert with the ability of the sperrnatozoa to capacitate. In this study, we have addressed the mechanisms by which spermatozoa gain the potential to activate this signaling pathway during epididymal maturation. In a modified Tyrode's medium containing 1.7 mM calcium, caput spermatozoa had significantly higher [Ca2+](i) than caudal cells and could not tyrosine phosphorylate in response to cAMP. However, in calcium-depleted medium both caput and caudal cells could exhibit a …

The Interaction Of Alphab-Crystallin With Mature Alpha-Synuclein Amyloid Fibrils Inhibits Their Elongation, Christopher A. Waudby, Tuomas P. J Knowles, Glyn L. Devlin, Jeremy N. Skepper, Heath Ecroyd, John A. Carver, Mark E. Welland, John Christodoulou, Christopher M. Dobson, Sarah Meehan

Heath Ecroyd

alphaB-Crystallin is a small heat-shock protein (sHsp) that is colocalized with alpha-synuclein (alphaSyn) in Lewy bodies—the pathological hallmarks of Parkinson's disease—and is an inhibitor of alphaSyn amyloid fibril formation in an ATP-independent manner in vitro. We have investigated the mechanism underlying the inhibitory action of sHsps, and here we establish, by means of a variety of biophysical techniques including immunogold labeling and nuclear magnetic resonance spectroscopy, that alphaB-crystallin interacts with alphaSyn, binding along the length of mature amyloid fibrils. By measurement of seeded fibril elongation kinetics, both in solution and on a surface using a quartz crystal microbalance, this binding …

Do You Believe The Climate Is Changing? Answers From New Survey Research, Lawrence C. Hamilton

The Carsey School of Public Policy at the Scholars' Repository

This brief explores how political views influence Americans’ understanding and perception of science. The research is based on a national version of the Community and Environment in Rural America survey called NCERA, and on New Hampshire’s statewide Granite State Poll. Author Lawrence Hamilton reports that most people on both surveys feel that they understand either a great deal or a moderate amount about global warming or climate change. However, deep partisan divisions affect both personal beliefs about climate change and perceptions of agreement among scientists. Democrats are much more likely to state they believe that climate change is happening, caused …

Investigating The Impact Of Restricted Irrigation Practices On Soil Moisture Variability And Distribution In A Dry Farmed Vineyard Site, Boise, Idaho, J. Duffin, David Wilkins, J. Guenther

David E. Wilkins

Changing climate in semiarid regions may result in increased water stresses for agricultural production as timing and form of precipitation may result in diminished surface water for irrigation. To prepare for these changing conditions, studies are being conducted on the possibility of dry farmed agriculture as an alternative to irrigated production. This study specifically investigates the ability to grow productive wine grapes with limited or zero irrigation in the Boise Front Foothills, West Foothills TIC Vineyard, located in a climate zone receiving less than 300 mm of annual precipitation. Traditional vineyard performance factors such as planting densities, soil type, rootstock, …

Sophomore Field Experiences As An Introduction To The Nature Of Geosciences: Data Collection And Analysis Using Dendrochronology, David Wilkins, M. Kunkel

David E. Wilkins

Opportunities for field experiences are often presented as a primary reason that undergraduate students gravitate towards geosciences as a major field of study. What those students may not understand is that, for professional geoscientists, field experiences go beyond the freshman-level field trip, and students may overlook the processes of observation, data collection and analysis that are inherent to the science. The Department of Geosciences at Boise State has developed a set of sophomore field experience courses designed to "set the hook" into new majors and prepare them for field experiences in upper division coursework. These sophomore courses have been shown …

Whole-Rock Lead-Lead Systematics And Major Element Analyses On The 1.85 Ga. Flin Flon Paleosol, Manitoba, Canada: Implications For Uranium Mobility., Federico Arturo Valencia

Geosciences Theses

The 1.85 Ga Flin Flon paleosol located in Flin Flon, Manitoba, Canada, is studied with the purpose of determining the timing and geochemical trend of uranium migration. Radiometric minimum ages of sediments and paleosols indicates the presence of a post-depositional event, these ages are bracketed by the Trans-Hudson orogeny event (2155–1750 Ma) which resulted in the alteration of κ(Th/U) and µ(U/Pb) ratios by exposing volcanics to the atmosphere and instigating the mobilization of U. The profile shows that the Missi sediments lost Uby 84% average relative to corrected average upper crust value. The upper paleosol gained U by 11% and …

Erasing Boundaries: Global Resource Sharing In The 21st Century, Robert A. Seal

Robert A Seal

Academic libraries have long been accustomed to participating in cooperative ventures with neighboring institutions as well as with those in other regions of their country. In large part, such activities have grown out of a desire to reduce or control costs, as well as to provide users with a broader base of materials for research, study, and teaching. Because not even a well-developed national library is able to fulfill all its users' needs all the time, today's university libraries must depend upon one another to fulfill those needs. For many decades, the resource sharing tradition has been particularly strong among …

If And How Many 'Races'? The Application Of Mixture Modeling To World-Wide Human Craniometric Variation, Bridget Frances Beatrice Algee-Hewitt

Doctoral Dissertations

Studies in human cranial variation are extensive and widely discussed. While skeletal biologists continue to focus on questions of biological distance and population history, group-specific knowledge is being increasingly used for human identification in medico-legal contexts. The importance of this research has been often overshadowed by both philosophic and methodological concerns. Many analyses have been constrained in their scope by the limited availability of representative samples and readily criticized for adopting statistical techniques that require user-guidance and a priori information. A multi-part project is presented here that implements model-based clustering as an alternative approach for population studies using craniometric traits. …

Minerva 2011, The Honors College

Minerva

This issue of Minerva includes an article on four newly-hired Honors preceptors, Rob Glover, Sarah Harlan-Haughey, Jordan LaBouff, and Justin Martin; a feature on Honors award, scholarship, and fellowship winners; and an article on the Honors College collaboration with the IDeA Network of Biomedical Research Infrastructure (INBRE) National Genomics Research Initiative.

Randomization Tests For Quantifying Species Importance To Ecosystem Function, Nicholas J. Gotelli, Werner Ulrich, Fernando T. Maestre

College of Arts and Sciences Faculty Publications

1. Quantifying the contribution of different species to ecosystem function is an important challenge. We introduce simple randomization tests (and software) for quantifying the average effect of species on ecosystem variables measured in multiple plots with and without the presence of a particular species. These randomization tests formalize the analysis of uncontrolled 'natural experiments' and quantify species effects in standardized deviation units. 2.We tested the method with data on ecosystem function in biological soil crust assemblages of lichens in semi-arid gypsum outcrops in central Spain. In sixty-three 50cm×50cm sample plots, we measured the presence and percentage cover of 17 species …

Underestimating The Costs Of Conservation In Southeast Asia, David P. Edwards, Brendan Fisher, Xingli Giam, David S. Wilcove

Rubenstein School of Environment and Natural Resources Faculty Publications

No abstract provided.

Overview Of Contrast Data Mining As A Field And Preview Of An Upcoming Book, Guozhu Dong, James Bailey

Kno.e.sis Publications

This report provides an overview of the field of contrast data mining and its applications, and offers a preview of an upcoming book on the topic. The importance of contrasting is discussed and a brief survey is given covering the following topics: general definitions and terminology for contrast patterns, representative contrast pattern mining algorithms, applications of contrast mining for fundamental data mining tasks such as classification and clustering, applications of contrast mining in bioinformatics, medicine, blog analysis, image analysis and subgroup mining, results on contrast based dataset similarity measure, and on analyzing item interaction in contrast patterns, and open research …

Missouri National Recreational River, Natural Resource Condition Assessment, Kevin J. Stark, Lucas J. Danzinger, Michael R. Komp, Andy J. Nadeau, Shannon Amberg, Eric J. Iverson, David Kadlec, Barry Drazkowski

United States National Park Service: Publications

Executive Summary

As a unit in the National Park Service (NPS), Missouri National Recreational River (MNRR) is responsible for the management and conservation of natural resources within its boundaries. This mandate is supported by the National Park Service Organic Act of 1916, which directs the NPS to:

conserve the scenery and natural and historic objects and the wildlife therein and to provide for the enjoyment of the same in such a manner and by such means as will leave them unimpaired for the enjoyment of future generations.

In 2003, NPS Water Resources Division received funding through the Natural Resource Challenge …

Scotts Bluff National Monument Plant Community Composition And Structure Monitoring, 2011 Annual Report, Isabel W. Ashton, Michael Prowatzke, Michael R. Bynum, Tim Shepherd, Stephen K. Wilson, Kara Paintner-Green

United States National Park Service: Publications

Executive Summary

The Northern Great Plains Inventory & Monitoring Network (NGPN) was established to develop and provide scientifically credible information on the current status and long-term trends of the composition, structure, and function of ecosystems in thirteen parks located in five northern Great Plains states. NGPN identified upland plant communities, exotic plant early detection, and riparian lowland communities as vital signs that can be used to better understand the condition of terrestrial park ecosystems (Gitzen et al. 2010). Upland and riparian ecosystems are important targets for vegetation monitoring because the status and trends in plant communities provide critical insights into …

Agate Fossil Beds National Monument Plant Community Composition And Structure Monitoring, 2011 Annual Report, Isabel W. Ashton, Michael Prowatzke, Michael R. Bynum, Tim Shepherd, Stephen K. Wilson, Kara Paintner-Green

United States National Park Service: Publications

Executive Summary

The Northern Great Plains Inventory & Monitoring Network (NGPN) was established to develop and provide scientifically credible information on the current status and long-term trends of the composition, structure, and function of ecosystems in thirteen parks located in five northern Great Plains states. NGPN identified upland plant communities, exotic plant early detection, and riparian lowland communities as vital signs that can be used to better understand the condition of terrestrial park ecosystems (Gitzen et al. 2010). Upland and riparian ecosystems are important targets for vegetation monitoring because the status and trends in plant communities provide critical insights into …

Designing Carbon Taxation Schemes For Automobiles: A Simulation Exercise For Germany, Adamos Adamou, Sofronis Clerides, Theodoros Zachariadis

Theodoros Zachariadis

Vehicle taxation based on CO2 emissions is increasingly being adopted worldwide in order to shift consumer purchases to low-carbon cars, yet little is known about the effectiveness and overall economic impact of these schemes. We focus on feebate schemes, which impose a fee on high-carbon vehicles and give a rebate to purchasers of low-carbon automobiles. We estimate a discrete choice model of demand for automobiles in Germany and simulate the impact of alternative feebate schemes on emissions, consumer welfare, public revenues and firm profits. The analysis shows that a well-designed scheme can lead to emission reductions without reducing overall welfare.

The Value Of Government Mandated Location-Based Services In Emergencies In Australia, Anas Aloudat, Katina Michael, Roba Abbas, Mutaz M. Al-Debei

Dr. Mutaz M. Al-Debei

The adoption of mobile technologies for emergency management has the capacity to save lives. In Australia in February 2009, the Victorian Bushfires claimed 173 lives, the worst peace-time disaster in the nation’s history. The Australian government responded swiftly to the tragedy by going to tender for mobile applications that could be used during emergencies, such as mobile alerts and location services. These applications, which are becoming increasingly accurate with the evolution of positioning techniques, have the ability to deliver personalized information direct to the citizen during crises, complementing traditional broadcasting mediums like television and radio. Indeed governments have a responsibility …

Computing Inconsistency Measure Based On Paraconsistent Semantics, Pascal Hitzler, Yue Ma, Guilin Qi

Computer Science and Engineering Faculty Publications

Measuring inconsistency in knowledge bases has been recognized as an important problem in several research areas. Many methods have been proposed to solve this problem and a main class of them is based on some kind of paraconsistent semantics. However, existing methods suffer from two limitations: (i) they are mostly restricted to propositional knowledge bases; (ii) very few of them discuss computational aspects of computing inconsistency measures. In this article, we try to solve these two limitations by exploring algorithms for computing an inconsistency measure of first-order knowledge bases. After introducing a four-valued semantics for first-order logic, we define an …

Perception Of Aquifer Depletion And The Effects Of Land Use Change Across The Madaba Plain, Jordan, Mohammad Salem

Graduate Theses and Dissertations

In Jordan, demand for water for agricultural purposes has put a high strain on aquifer resources. Water mining, erosion, and desertification have all increased as global temperatures rise. This along with fragmentation of the landscape have altered the environment in a profound way. The Madaba Plain was chosen for this study due to the agricultural activities that take place there, as well as the proximity to a number of population centers. The purpose of the study is to examine how fragmentation of irrigated landscape has affected the aquifers underneath, while taking into account perceptions of risk of the local population. …

The Impact Of ‘‘No Impact Man’’: Alternative Hedonism As Environmental Appeal, Jen Schneider, Glen Miller

Jen Schneider

As ‘‘No Impact Man,’’ writer Colin Beavan conducted a one-year experiment to determine whether he and his family could reduce their environmental impact to zero while living and working in Manhattan. This article examines the No Impact Man (NIM) experiment both as ‘‘alternative hedonism,’’ a reconceptualization of the ‘‘good life’’ that avoids unduly damaging the natural world, and also as a kind of ‘‘eco-stunt,’’ an attempt to garner significant media coverage about positive environmental behaviors. We use DeLuca’s theorization of the ‘‘image event’’ to analyze the No Impact Man franchise—blog, book, and documentary film—though we modify that theory in order …

Object Recognition Based On Shape And Function, Akihiro Eguchi

Computer Science and Computer Engineering Undergraduate Honors Theses

This thesis explores a new approach to computational object recognition by borrowing an idea from child language acquisition studies in developmental psychology. Whereas previous image recognition research used shape to recognize and label a target object, the model proposed in this thesis also uses the function of the object resulting in a more accurate recognition. This thesis makes use of new gaming technology, Microsoft’s Kinect, in implementing the proposed new object recognition model. A demonstration of the model developed in this project properly infers different names for similarly shaped objects and the same name for differently shaped objects.

Spatial Analysis Of Fatal Automobile Crashes In Kentucky, William Nathan Oris

Masters Theses & Specialist Projects

Fatal automobile crashes have claimed the lives of over 33,000 people each year in the United States since 1995. As in any point event, fatal crash events do not occur randomly in time or space. The objectives of this study were to identify spatial patterns and hot spots in FARS (Fatal Analysis Reporting System) fatal crash events based on temporal and demographic characteristics. The methods employed included 1) rate calculation using FARS points and average daily traffic flow; 2) planar kernel density estimation of FARS crash events based on temporal and demographic attributes within the data; and 3) two case …

Multiple Storm Event Impacts On Epikarst Storage And Transport Of Organic Soil Amendments In South-Central Kentucky, Sean M. Vanderhoff

Masters Theses & Specialist Projects

The groundwater in agricultural karst areas is susceptible to contamination from organic soil amendments and pesticides. During major storm events during 2011, dye traces were initiated using sulphorhodamine-B, fluorescein and eosine in a groundwater recharge area where manure was applied to the ground. Fecal coliform samples were collected from significant storm events from January-September 2011. Water samples and geochemical data were collected every four hours before, during, and between the storm events from a waterfall in Crumps cave flowing from the known recharge area to track the transport and residence time of the epikarst water and organic soil amendments during …

Geospatial Tools And Techniques For Watershed Management Using Swat 2009, Naresh Pai

Graduate Theses and Dissertations

In this study, geospatial tools and techniques were developed to support specific aspects of watershed pollution management, such as quantifying land-use change (LUC) impacts, prioritizing subwatersheds, and communicating field-scale impacts, using the soil and water assessment tool 2009 (SWAT 2009) model.

For the land-use change objective, a geospatial tool titled SWAT2009_LUC was developed that enables SWAT modelers to prepare specific input files for simulating concurrent land-use changes during the SWAT 2009 model simulations. Testing of the tool for the Illinois River Drainage Area in Arkansas (IRDAA) watershed showed that the tool accurately represented temporal land-uses within the model. Model simulations …