Physical Sciences and Mathematics Commons^™

Comparison Of Virulence Gene Profiles Between Escherichia Coli Strains Isolated From Healthy And Diarrheic Swine, Mark Wilson, Karl A Bettelheim, X Y Wu, S Driesen, James Chin, Toni Chapman, I Barchia, D Trott

Mark R Wilson

No abstract provided.

Amyloid Fibril Formation By Bovine Milk Kappa-Casein And Its Inhibition By The Molecular Chaperones Alpha-S And Beta-Casein, Mark Wilson, David Thorn, Agata Rekas, S. L Gras, Christopher Dobson, Sarah Meehan, Cait Macphee, M Sunde

Mark R Wilson

No abstract provided.

Heat Shock Protein 70 Inhibits Alpha-Synuclein Fibril Formation Via Preferential Binding To Prefibrillar Species, Mark Wilson, M. M. Dedmon, J. Christodoulou, Christopher Dobson

Mark R Wilson

No abstract provided.

Lymphotoxin-Beta Receptor-Dependent Genes In Lymph Node And Follicular Dendritic Cell Transcriptomes, Mark Wilson, C. Huber, C. Thielen, Y. Fu, E. Heinen, A Aguzzi, G Miele

Mark R Wilson

No abstract provided.

The Acute Phase Protein Haptoglobin Is A Mammalian Extracellular Chaperone With An Action Similar To Clusterin, Justin Yerbury, Mark S Rybchyn, Simon B Easterbrook-Smith, C. Henriques, Mark Wilson

Mark R Wilson

Haptoglobin (Hp) is an acidic glycoprotein present in most body fluids of humans and other mammals. Although the functions of Hp are not yet fully understood, the available evidence indicates that it is likely to play an important role in suppressing inflammatory responses. Some earlier work suggested that Hp might be a newly identified member of a small group of extracellular chaperones found at significant levels in human body fluids. Previously, the only well-characterized member of this group was clusterin, which shares functional similarities with the small heat-shock proteins. We report here that Hp specifically inhibited the precipitation of a …

Knowledge Discovery From Multispectral Satellite Images, Arun D. Kulkarni

Arun Kulkarni

Using Information Literacy Standards To Improve Geoscience Courses, Aimee Dechambeau, Ira Sasowsky

Aimee deChambeau

Information literacy (IL) is the set of skills that enables a person to find, evaluate, and use information. Although IL is important in the geosciences, it is hard to teach because the information comes in so many forms. Most geoscience courses include work that implicitly addresses some IL issues. However, because of the importance of IL, it is worthwhile to explicitly include it as part of the curriculum. The Association of College and Research Libraries has developed Information Literacy Standards for Higher Education that may be used to assess an individual's level of information literacy. We introduce a method for …

Working Across Cultures, John Hooker

John Hooker

No abstract provided.

Detecting Mitochondrial Permeability Transition By Confocal Imaging Of Intact Cells Pinocytically Loaded With Calcein, Rachel Jones, Alison Smail, Mark Wilson

Mark R Wilson

When studied in vitro, mitochondrial permeability transition (MPT) is associated with an increase in mitochondrial permeability to solutes up to 1500 Da in mass and a loss of electrical potential difference across the inner mitochondrial membrane (delta psi mit). The MPT has been implicated as being important in cellular calcium homeostasis, autophagy and cell death via necrosis and apoptosis. Thus, it is important to develop a valid technique for accurate measurement of this phenomenon in intact cells. We developed a procedure for the detection of MPT in intact cells that avoids disadvantages associated with earlier approaches. In this new technique, …

Evidence That Clusterin Has Discrete Chaperone And Ligand Binding Sites, Johnathon Lakins, Stephen Poon, Simon Easterbrook-Smith, John Carver, Martin Tenniswood, Mark Wilson

Mark R Wilson

Clusterin is the first identified extracellular mammalian chaperone and binds to a wide variety of partly unfolded, stressed proteins. Clusterin also binds to many different unstressed ligands including the cell surface receptor low density lipoprotein receptor-related protein-2 (LRP-2). It is unknown whether clusterin binds to all of these many ligands via one or more binding sites. Furthermore, the region(s) of clusterin involved in these many binding interactions remain to be identified. As part of an investigation of these issues, we expressed recombinant human clusterin in the yeast Pichia pastoris. The resultant protein had variable proteolytic truncations of the C-terminal region …

Clusterin Is An Atp-Independent Chaperone With Very Broad Substrate Specificity That Stabilizes Stressed Proteins In A Folding-Competent State, Stephen Poon, Simon Easterbrook-Smith, Mark Rybchyn, John Carver, Mark Wilson

Mark R Wilson

We recently reported that the ubiquitous, secreted protein clusterin has chaperone activity in vitro [Humphreys et al. (1999) J. Biol. Chem. 274, 6875−6881]. In this study, we demonstrate that clusterin (i) inhibits stress-induced precipitation of a very broad range of structurally divergent protein substrates, (ii) binds irreversibly via an ATP-independent mechanism to stressed proteins to form solubilized high molecular weight complexes, (iii) lacks detectable ATPase activity, (iv) when acting alone, does not effect refolding of stressed proteins in vitro, and (v) stabilizes stressed proteins in a state competent for refolding by heat shock protein 70 (HSP70). Furthermore, we show that, …

Logic-Based Methods For Optimization: Combining Optimization And Constraint Satisfaction, John Hooker

John Hooker

No abstract provided.

Radiocarbon Chronology And Δ13c Analysis Of Mid-To Late-Holocene Aeolian Environments, Guadalupe Mountains National Park, Texas, Usa, David Wilkins, Donald Currey

David E. Wilkins

The Red Dunes of Guadalupe Mountains National Park are quartzose sand sheets and dunes stabilized by sparse plant cover. Stratigraphy, radiocarbon dating, and δ13C analysis were examined in two of the larger arroyos for correlating evidence of aeolian deposition, which in this basin is taken as an indication of arid conditions. Four quartz-sand lithostratigraphic units, representing intervals of aeolian activity, are identified. Radiocarbon dates provide constraint on the timing of the three youngest quartz-sand units. A mid-Holocene aeolian interval, represented by a single radiocarbon date of 6350± 70 BP, is interpreted as transitional to an altithermal regime. A neopluvial period, …

Optimization Methods For Logical Inference, Vijay Chandru, John Hooker

John Hooker

No abstract provided.

Longitudinal Study Of Dental Development In Chimpanzees Of Known Chronological Age: Implications For Understanding The Age At Death Of Plio-Pleistocene Hominids, Robert Anemone, Mark Mooney

Robert L. Anemone

Reconstruction of life history variables of fossil hominids on the basis of dental development requires understanding of and comparison with the pattern and timing of dental development among both living humans and pongids. Whether dental development among living apes or humans provides a better model for comparison with that of Plio-Pleistocene hominids of the genus Australopithecus remains a contentious point. This paper presents new data on chimpanzees documenting developmental differences in the dentitions of modern humans and apes and discusses their significance in light of recent controversies over the human or pongid nature of australopithecine dental development. Longitudinal analysis of …

Timing And Extent Of Late Quaternary Paleolakes In The Trans-Pecos Closed Basin, West Texas And South-Central New Mexico, David Wilkins, Donald Currey

David E. Wilkins

The Trans-Pecos Closed Basin is a hydrographically closed region covering 20,000 km centered on Salt Basin, 160 km east of El Paso, Texas. Geomorphic and limnetic evidence have been used to identify four major highstands for Lake King during the last glacial maximum (LGM). Additional geomorphic features from a second, recently identified, paleolake, Lake Sacramento, have been found in the Beargrass subbasin, a nested subbasin approximately 75 km northwest of Salt Basin. Radiocarbon ages of the organic material in Lake King sediments date four abrupt climate changes and rapid lacustrine transgressions during the LGM with a quasi-periodicity of 2000 yr. …

The Vcl Hypothesis Revisited: Patterns Of Femoral Morphology Among Quadrupedal And Saltatorial Prosimian Primates, Robert Anemone

Robert L. Anemone

The descriptive and functional morphology of the postcranium of the vertical clinging and leaping prosimians is of great interest in both adaptational and phylogenetic studies of extant and extinct primates. An analysis of patterns of femoral morphology among quadrupedal and saltatory living prosimians indicates the presence of at least two, and possibly three, distinct femoral adaptations to the demands of an arboreal, saltatory existence. Osteological measurements were taken on 277 postcranial skeletons representing eight prosimian families, with skeletal trunk length (Biegert and Maurer, Folia Primatol. 17:142–156, 1972) used as an estimator of body size in both bivariate and multivariate (discriminant …