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Characterization Of Α‑Synuclein Multimer Stoichiometry In Complex Biological Samples By Electrophoresis, Bryan A. Killinger, Anna Moszczynska
Characterization Of Α‑Synuclein Multimer Stoichiometry In Complex Biological Samples By Electrophoresis, Bryan A. Killinger, Anna Moszczynska
Pharmaceutical Sciences Faculty Publications
The aberrant aggregation of α-synuclein in the brain is a hallmark of Parkinson’s disease (PD). In vivo soluble α-synuclein occurs as a monomer and several multimers, the latter of which may be important for the biological function of α-synuclein. Currently, there is a lack of reproducible methods to compare α-synuclein multimer abundance between complex biological samples. Here we developed a method, termed “multimer-PAGE,” that combines in-gel chemical cross-linking with several common electrophoretic techniques to measure the stoichiometry of soluble α-synuclein multimers in brain tissue lysates. Results show that soluble α-synuclein from the rat brain exists as several high molecular weight …