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Weast Nile Virus Serosurveillance In Iowa White-Tailed Deer (1999-2003), Julian Santatella, Robert Mclean, Jeffrey S. Hall, James S. Gill, Richard A. Bowen, Harlo H. Hadow, Larry Clark

USDA Wildlife Services: Staff Publications

Sera from white-tailed deer (Odocoileus virginianus) were collected in Iowa during the winter months (1999-2003), 2 years before and after West Nile virus (WNV) was first reported in Iowa (2001), and were analyzed for antibodies to WNV. Samples from 1999 to 2001 were antibody negalive by blocking enzyme-linikedI immunosorbent assay (bELISA) and plaque reduction neutralization test (PRNT₉₀). Prevalence derived from bELISA (2002, 12.7%; 2003. 11.2%) and WNV PRNT₉₀ (2002,7.9%; 2003, 8.5%) assays were similar. All sanlples were negative for antibodies against St. Louis encephalitis virus as determined by PRNT₉₀. Antibodies to flaviviruses were …