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Physical Sciences and Mathematics Commons

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Full-Text Articles in Physical Sciences and Mathematics

Kinetic Characterization Of Listeria Monocytogenes 2-C- Methyl-D-Erythritol 4-Phosphate Cytidylyltransferase ( Cms ) Enzymes Using High Performance Liquid Chromatography ( Hplc ), Mark Oblazny Oct 2017

Kinetic Characterization Of Listeria Monocytogenes 2-C- Methyl-D-Erythritol 4-Phosphate Cytidylyltransferase ( Cms ) Enzymes Using High Performance Liquid Chromatography ( Hplc ), Mark Oblazny

Theses and Dissertations

Infectious diseases, with increasing prevalence of antibiotic resistant bacteria, coupled with the declining rate in discovery of antimicrobial agents, impose one of the most significant threats to human health. Here we identify 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (CMS) as a valid target for antibiotic development which is an enzyme in pathogenic organisms that leads to the biosynthesis of isoprene precursor molecules. Isoprene molecules are one of nature’s most common building blocks that are vital to many biological metabolic processes and are synthesized via the mevalonic acid dependent (MVA), or methylerythritol phosphate (MEP) pathway. Vertebrates utilize the MVA pathway, while many pathogenic bacteria …


The Biochemical Assessment Of Two Secreted Acid Phosphatases From Leishmania Tarentolae, Their Response To Electric Fields, Glycosidase Incubation, And / Or Vanadium, Benjamin M. Dorsey Oct 2017

The Biochemical Assessment Of Two Secreted Acid Phosphatases From Leishmania Tarentolae, Their Response To Electric Fields, Glycosidase Incubation, And / Or Vanadium, Benjamin M. Dorsey

Theses and Dissertations

Leishmaniasis, as defined by the Center for Disease Control and Prevention, is a neglected tropical disease with 1.6 million new cases reported each year. However, there is yet to be safe, effective, and affordable treatments provided to those affected by this disease1. Still underappreciated as a potential pharmaceutical targets, especially for cutaneous leishmaniasis infections, are the two isozymes of secreted acid phosphatase (SAP); secreted acid phosphatase 1 (SAP1) and secreted acid phosphatase 2 (SAP2). These enzymes are involved in the survival of the parasite in the sand fly vector, and the prevention of host macrophages from forming parasitophorous vacuole and …


Nutrient Loading Reduction In A Tile Drained Agricultural Watershed Through Watershed-Scale Cover Cropping: A High Resolution Analysis, Benjamin Gerald Bruening Apr 2017

Nutrient Loading Reduction In A Tile Drained Agricultural Watershed Through Watershed-Scale Cover Cropping: A High Resolution Analysis, Benjamin Gerald Bruening

Theses and Dissertations

Nutrient pollution originating from agricultural regions in the Midwest is a serious issue, leading to pollution of drinking water sources as well as large hypoxic zones in the Gulf of Mexico. The source of much of this contamination has been shown to be runoff from agricultural fields in the Upper Mississippi River Basin. One method that has been shown to reduce this pollution from the Upper Mississippi River Basin is the planting of winter cover crops. Winter cover crops such as rye and tillage radish have been shown to significantly reduce nitrate exported from agricultural fields, even in tile-drained watersheds …


Creating A Scaling Relationship Between Peak Discharge And Drainage Area To Identify Tile Drainage Inputs Into An Agricultural Stream, Ryan Plath Apr 2017

Creating A Scaling Relationship Between Peak Discharge And Drainage Area To Identify Tile Drainage Inputs Into An Agricultural Stream, Ryan Plath

Theses and Dissertations

Tile drains have been shown to contribute to high levels of nitrate in agricultural streams. Locations of tile drains on a watershed scale, however, are often unknown due to tile drains being located on many separate parcels of private property. This study evaluates the ability of a methodology, using scaling relationships between discharge and drainage area, for locating areas of large tile drainage contribution to Money Creek, in McLean County, Illinois. Additionally, this study examines the difference in scaling relationships and physical stream hydrology between tileflow and no-tileflow conditions. Eight stream sites were created in the watershed, that recorded stage …