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Oxidative Stress In Algae: Method Development And Effects Of Temperature On Antioxidant Nuclear Signaling Compounds, Md Noman Siddiqui

Open Access Theses

Non-enzymatic antioxidants neutralize the oxidative stress through scavenging reactive oxygen species. The structural complexity of non-enzymatic nuclear signaling antioxidants poses are more challenges for traditional quantitative analysis as these compounds have multiple forms and structurally diverse than proteins and therefore, challenge traditional analytical techniques. Current analytical methods cannot distinguish the carotenoids, and vitamin A and D metabolites within a single sample. To find an appropriate method to measure all the nuclear signaling compounds in algae, Isochrysis sp. , under thermal stress, HPLC/ESI-MS and HPLC /APPI-MS was used. Standards of those compounds were run by QQQ HPLC/ ESI-MS and HPLC /APPI-MS …