Physical Sciences and Mathematics Commons^™

New Strategies To Reveal Protein Candidates In Protein-Protein Interactome Study, Meng-Chieh Chen

Open Access Theses

Comprehensive protein-protein interaction network analysis can help reveal protein functions in a system-wide manner. A reliable knowledgebase of interaction networks is not only important for selecting the candidates for drug therapies, but also for evaluating the disease risk. In current interaction databases, 322579 interactions comprised of 56460 proteins have been reported (statistical analysis from APID: Agile Protein Interaction DataAnalyzer; http://bioinfow.dep.usal.es/apid/index.htm). The huge datasets are contributed mainly by yeast -two -hybrid (Y2H) screening and affinity-purification followed by mass spectrometry (AP-MS). High false positive rates and failing to cover certain interaction categories are the limitations of these two methods. Here, we developed …

Liquid Swine Manure Application Timing And Instincttm Impacts On Net Soil Nitrogen Mineralization And Corn Yield, Min Xu

Open Access Theses

Nitrogen (N) is one of the most expensive variable input costs for corn production. Optimized manure management is essential to ensure maximum crop N utilization and to reduce the risk of N loss. A field study was conducted in central Indiana from 2011-2013 to assess liquid swine ( Sus scrofa L.) manure N availability when applied at different times or co-applied with InstinctTM , a microencapsulated form of nitrapyrin. Soil inorganic N contents, soil N mineralization, corn yield, grain N content, whole plant N concentrations of corn at the V6 growth stage, and ear leaf N concentrations of corn at …

Assessing Positional Accuracy And Correcting Point Data For Digital Soil Mapping At Varying Scales, Minerva J. Dorantes

Open Access Theses

Accuracy, timeliness, and the effect of scale of soil maps are rarely assessed. The recent increase in the use of GIS technologies and modelling software in natural resources and land management, has increased the demand for soil information at a finer resolution worldwide. Most of the world's developing countries rely on soils information at a scale that is too coarse for practical planning, and have obstacles impeding collection of new data, such as civil war and a lack of collection resources. The United States has an exhaustive collection of soils data at a fine scale. However, its location information is …

Vegetation And Soil Characteristics Of Pine Plantations And Naturally Regenerated Hardwood Forests On The Hoosier National Forest, Patrick James Duffy

Open Access Theses

During the 1930s there was widespread erosion on farmland and subsequent land abandonment. As a result, Pinus strobus L. (white pine), P. resinosa Aiton (red pine), and P. echinata Mill. (shortleaf pine) were planted in the Midwest to prevent erosion and rehabilitate sites. These species were selected due to their wide availability at the time. Currently, it is the goal of the U.S. Forest Service to provide a more natural and sustainable landscape, in part by removing these non-native Pinus stands and by replacing them with native hardwood species. The ultimate success of hardwood restoration depends, in part, on the …

Cosmogenic Beryllium Cycling In A Natural Forest Setting, Grace Conyers

Open Access Theses

10 Bemet , or cosmogenic beryllium, has a long half-life of 1.4 million years and quick adsorption on soil particles, which may make it ideal for dating soil erosion in historical context. However, there are questions on about the fundamental assumptions of the retentivity of 10 Bemet . This manuscript explores these assumptions and the context of nutrient cycling in a natural forest setting.

To see if 10 Bemet was being cycled through the trees, and at what rate, we looked at the[10 Bemet ] in the soil, 4 species of trees, and their leaves. The isotopic ratio 10 Be/9 …

Deforestation Of Cloud Forest In The Central Highlands Of Guatemala: Soil Erosion And Sustainability Implications For Q'Eqchi' Maya Communities, Ian Christopher Pope

Open Access Theses

Understanding the nexus between deforestation, food production, land degradation, and culture contributes knowledge that is useful for development practitioners working to enhance conservation and food security. Documenting deforestation and soil erosion in the Sierra Yalijux and Sierra Sacranix in the Central Highlands of Guatemala adds new knowledge about the rates and dynamics of deforestation and land degradation in areas with unique and sensitive cloud forest ecosystems. It also suggests possible areas of emphasis for efforts targeted at combining cloud forest conservation with sustainability for indigenous Q'eqchi' communities. In addition, this work contributes to a small but growing body of literature …

Oxidative Stress In Algae: Method Development And Effects Of Temperature On Antioxidant Nuclear Signaling Compounds, Md Noman Siddiqui

Open Access Theses

Non-enzymatic antioxidants neutralize the oxidative stress through scavenging reactive oxygen species. The structural complexity of non-enzymatic nuclear signaling antioxidants poses are more challenges for traditional quantitative analysis as these compounds have multiple forms and structurally diverse than proteins and therefore, challenge traditional analytical techniques. Current analytical methods cannot distinguish the carotenoids, and vitamin A and D metabolites within a single sample. To find an appropriate method to measure all the nuclear signaling compounds in algae, Isochrysis sp. , under thermal stress, HPLC/ESI-MS and HPLC /APPI-MS was used. Standards of those compounds were run by QQQ HPLC/ ESI-MS and HPLC /APPI-MS …

Study Of The Sustainbility Issues Of Food Production Using Vertical Farm Methods In An Urban Environment Within The State Of Indiana, Victor Mendez Perez

Open Access Theses

There are several problems around the globe related to water scarcity, energy shortages, deforestation and loss of fertile land, and food security. Climate change is making environmental and social issues arise. Issues such as, changes in the agricultural landscape, increasing population (9 billion is the expected population for 2040), rising food borne illnesses, scarcity of drinking water, and more crop failure due to plant pathogens and insect pests, among other factors make this study specially significant. Vertical farming could be a potencial solution to some of these problems.

Due to these problems the present thesis analyzed vertical farming as a …

Pharmaceuticals And Personal Care Products: Emerging Contaminants In Aquatic Ecosystems, Jenny E. Zenobio

Open Access Theses

In recent years, the presence of pharmaceuticals and personal care products (PPCPs) in aquatic systems has led to research on their fate and effects. PPCPs have been found in mixture in wastewater effluents, surface, ground, and drinking water at low concentrations from areas of intense urbanization. Although adverse effects to human health from the current environmental concentrations are unlikely, the impacts to ecological receptors are not clear. We performed field and laboratory studies to quantify and evaluate effects of PPCPs on fish. First, a field study was conducted at the Baca National Wildlife Refuge, Colorado (2010-2012) because a portion of …