Physical Sciences and Mathematics Commons^™

Synthesis And Characterization Of Amphiphiles For Mammalian Cell Transfection And Antimicrobial Activity, Joseph William Meisel

Dissertations

Amphiphiles are molecules that contain both water-soluble and water-insoluble components. The dual nature of these molecules engenders remarkable properties including the ability to self-assemble into ordered structures. Cell membranes are composed of amphiphilic phospholipids that organize into a bilayer motif. Synthetic amphiphiles can interact with natural membranes and influence the transport of molecules across the cell membrane. The work elaborated in this report employs amphiphiles to co-assemble with DNA and transport the genetic material across cell membranes. First, a simplified method for interacting DNA with amphiphiles was developed. Second, a series of known ion-transporting compounds were assayed for their interaction …

Unraveling The Mechanism Of Selective Ion Transport In Hydrophobic Subnanometer Channels, Hui Li, Joseph S. Francisco, Xiao Cheng Zeng

Xiao Cheng Zeng Publications

Recently reported synthetic organic nanopore (SONP) can mimic a key feature of natural ion channels, i.e., selective ion transport. However, the physical mechanism underlying the K⁺/Na⁺ selectivity for the SONPs is dramatically different from that of natural ion channels. To achieve a better understanding of the selective ion transport in hydrophobic subnanometer channels in general and SONPs in particular, we perform a series of ab initio molecular dynamics simulations to investigate the diffusivity of aqua Na⁺ and K⁺ ions in two prototype hydrophobic nanochannels: (i) an SONP with radius of 3.2 Å, and (ii) single-walled …

Equipment Qualification Of Ion Mobility Spectrometry And Method Development And Validation For Pharmaceutical Equipment Cleaning Validation, Amy Lee Heiser

Masters Theses

Ion mobility spectrometry (IMS) instrumentation has been identified as a suitable technology for the detection and reporting of drug product and detergent residues from pharmaceutical manufacturing equipment. Ion mobility is not a new technology, but is entering the field of cleaning validation because of tightened requirements from the US Food and Drug Administration (FDA).

The purpose of this thesis is to outline a practical implementation of the analytical technique, Ion Mobility Spectrometry in a cleaning validation program. Ion Mobility Spectrometry (IMS) is fast and specific for the analysis of small organic molecules and has been gaining popularity in the pharmaceutical …

Prediction Of Metal Charge-Dependent Amide Hydrogen Exchange Rates For The Proteins Rubredoxin And Azurin, Stephen Scoglio

Honors Theses

Although it has been shown that the hydrogen exchange rates between the most slowly exchanging amide protons of a protein and the solvent can be used to predict the global thermodynamic stability of a protein, the great majority of amide hydrogens along the backbone of a protein exchange from either partially or fully folded conformations, and their rate of exchange strongly depends upon the environment in that folded state. We are examining the electrostatic and conformational dependence of the hydrogen exchange kinetics of Pseudomonas aeruginosa azurin, which can bind either the diamagnetic Cu(I) or Zn(II) in its active site and …

Synthesis And Applications Of Polycationic Organic Salts, Collaborative Project

Dyson College- Seidenberg School of CSIS : Collaborative Projects and Presentations

This entry adheres to the use of the quad chart template to provide a succint description only of the current research project undertaken by the participants. It provides for the following information

1. Participants and Affiliations
2. Overall Project Goals
3. Illustrative Picture
4. Specific research/artistic/pedagogic foci

Velocity And Temperature Characterization Of The First Vacuum Stage Expansion In An Inductively Coupled Plasma - Mass Spectrometer, William Neil Radicic

Theses and Dissertations

The inductively coupled plasma - mass spectrometer (ICP-MS) is the analytical instrument of choice for trace element detection and quantification. Despite the popularity of ICP-MS, significant degradation in sensitivity and precision occurs as the result of matrix and instrument-induced effects. The sources of these effects are not well understood, characterized, or correlated to particular plasma operating condition settings or matrix compositions and involve both neutral and charged species. The purpose of this study is to characterize the behavior of metastable Ar (I) atom and Ca (II) ion through the measurement of Doppler velocities and fluorescence line width "temperatures."

For the …

The Photochemical Reduction Of Octacyanomolybdate(V) Ion In Aqueous Solutions, Gordon W. Gray

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The photochemical reactions occurring in aqueous solutions of octacyanomolybdate(V) ion, Mo(CN)₈^3-, during irradiation with light of 365 and 436 nm wavelengths were studied. The quantum yields and reaction products were determined for neutral, unbuffered solutions as well as solutions buffered at pH 4.0, 7.0, and 9.0.

The results of these studies showed that the Mo(CN)₈^3- ion is rapidly reduced by light to a Mo(IV) complex. In neutral or acidic media, the initial photoreduction product was found to be the red heptacyanoaquomolybdate(IV) ion, Mo(CN)₇(H₂O)^3-. Photolysis in basic solution gave the …

Differentiation Between The Ph Effect And The Bicarbonate Ion Effect In Causing Lime-Induced Chlorosis, Hyrum Del Var Petersen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Lime-induced chlorosis has been recognized for many years as a problem where plants are grown on calcareous soils. There are many factors associated with and influencing this form of iron chlorosis and because of this it has been very difficult to determine the relationship between the factors and chlorosis.