Physical Sciences and Mathematics Commons^™

A Prostaglandin Synthase Inhibition Assay With Detection By Elisa, Patricia Scott

Theses & Honors Papers

A simple, reliable method is described for the routine measurement of non-steroidal anti-inflammatory drugs (NSAIDs) as inhibitors of prostaglandin synthase. Appropriate concentrations of inhibitor are incubated with ram seminal vesical prostaglandin synthase, sodium arachidonate and reduced glutathione. Prostaglandin E₂ (PGE₂) production is then quantitated by and enzyme linked immunosorbant assay (ELISA), in which anti-PGE₂ antibody is utilized in a binding competition between test sample and adsorbed conjugate of PGE₂-BSA. The antibody which remains bound to the solid phase is quantitatively determined colorimetrically by incubation with horse radish peroxidase labeled goat anti-rabbit IgG followed by …