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Apparent Retention Volume Variation With Flow Rate Change In High Performance Liquid Chromatography, Susanne Buntz Erz
Apparent Retention Volume Variation With Flow Rate Change In High Performance Liquid Chromatography, Susanne Buntz Erz
Seton Hall University Dissertations and Theses (ETDs)
Recent studies have demonstrated noticeable flow rate dependency of the chromatographic zone retention volume with respect to migration within the empty capillary. This appears to be the result of superposition of asymmetric lateral diffusion and of the laminar flow profile. Although these effects have been studied on empty capillaries, in the presence of the packed column, the retention shift may be insignificant relative to the adsorption-based retention of the analytes. In the case of fast and ultrafast HPLC with short capillary columns, the effect of extra-column caused variation in the analyte retention may constitute an increase of up to 120 …