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Full-Text Articles in Physical Sciences and Mathematics

Biochemical Characterization Of The Nifb Enzyme And Nifb-Cofactor, Jirair Gevorkyan Jan 2013

Biochemical Characterization Of The Nifb Enzyme And Nifb-Cofactor, Jirair Gevorkyan

Electronic Theses and Dissertations

The Mo-nitrogenase complex is composed of two components, Fe-protein and MoFe-protein. This complex is able to catalyze the reduction of N2 through the MgATP dependent transfer of electrons from the Fe-protein Fe4S4 cluster to the MoFe-protein P-cluster and, subsequently, to the iron-molybdenum cofactor (FeMoco). FeMo-co is a Fe7S9MoC-(R)-homocitrate cluster and has two biosynthetic precursors, NifB-co and L-cluster, of unknown structure and composition. The biosynthesis of FeMo-co is an enigmatic process that minimally requires NifB, NifEN, Fe-protein, MoO4 2- , (R)-homocitrate and S-adenolsylmethionine. A means to isolate the NifB enzyme for characterization has been developed through use of a GST-fusion tag. …


Remediation Of Polychlorinated Biphenyl (Pcb) Contaminated Building Materials Using Non-Metal And Activated Metal Treatment Systems, Tamra Legron-Rodriguez Jan 2013

Remediation Of Polychlorinated Biphenyl (Pcb) Contaminated Building Materials Using Non-Metal And Activated Metal Treatment Systems, Tamra Legron-Rodriguez

Electronic Theses and Dissertations

PCBs are recalcitrant compounds of no known natural origin that persist in the environment despite their ban by the United States Environmental Protection Agency in 1979 due to negative health effects. Transport of PCBs from elastic sealants into concrete, brick, and granite structures has resulted in the need for a technology capable of removing these PCBs from the materials. This research investigated the use of a nonmetal treatment system (NMTS) and an activated metal treatment system (AMTS) for the remediation and degradation of PCBs from concrete, brick, and granite affixed with PCB-laden caulking. The adsorption of PCBs onto the components …


Development Of Novel Redox Sensors And Processes Towards Biological Applications, Jigna Patel Jan 2013

Development Of Novel Redox Sensors And Processes Towards Biological Applications, Jigna Patel

Electronic Theses and Dissertations

Research on the cure and early detection of diseases such as diabetes, Alzheimer's, and Parkinson's is becoming of great interest due to the increasing number of people affected by them every year. An accurate and quick detection of various damaging species is highly critical in treatments of such diseases not only for exploring possible cures but also for early detection. If these diseases are detected during the initial stages than the possibility of curing them is much higher. Motivated by this, many researchers today have developed numerous types of sensing devices that can detect various physiological and biological compounds. However, …


Magnesium And Acidified Ethanol Based Treatment Systems For The Extraction And Dechlorination Of Polychlorinated Biphenyls From Contaminated Oils, Paints, And Soils, Simone Novaes-Card Jan 2013

Magnesium And Acidified Ethanol Based Treatment Systems For The Extraction And Dechlorination Of Polychlorinated Biphenyls From Contaminated Oils, Paints, And Soils, Simone Novaes-Card

Electronic Theses and Dissertations

Polychlorinated biphenyls (PCBs) are a class of environmentally persistent halogenated organic compounds that were once used as stabilizers to improve the properties of a variety of materials such as lubricants, heat transfer fluids, paints, and caulking materials. PCBs are also capable of migration through processes such as spillage into soils, leaching into groundwater, and volatilization into the atmosphere. Although banned in 1979 over health concerns, PCBs persist in these materials to this day because they are resistant to biotic degradation and natural weathering processes. The wide variety of contaminated materials means that many existing treatment options cannot be used across …