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Method Development For The Quantification Of Heavy Metals In Biological Matrices And Its Application To Clinical And Environmental Samples, Michelle Catherine Gende

Dissertations

A three-fold approach was taken to develop a method for the detection of heavy metals in different matrices and had to be performed sequentially. The first was to develop a method capable of quantifying lead in complex matrices by graphite furnace atomic absorption (GFAA). The developed method needed to meet specific analytical requirements, namely, to be robust, sensitive, and accurate. Initially, four different sample preparation methods were explored for the quantification of lead using Escherichia coli (E. coli) as a sample matrix. The sample preparation procedures attempted were acidic dilution, matrix modification, conventional heating digestion, and microwave assisted acid digestion …