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Articles 31 - 60 of 80
Full-Text Articles in Physical Sciences and Mathematics
Lead Optimization Of Dual Tubulin And Hsp27 Inhibitors, Bo Zhong, Rati Lama, Daniel G. Kulman, Bibo Li Ph.D., Bin Su Ph.D.
Lead Optimization Of Dual Tubulin And Hsp27 Inhibitors, Bo Zhong, Rati Lama, Daniel G. Kulman, Bibo Li Ph.D., Bin Su Ph.D.
Chemistry Faculty Publications
Tubulin and heat shock protein 27 (Hsp27) are well-characterized molecular targets for anti-cancer drug development. We previously identified lead compounds that inhibited both Hsp27 and tubulin. These compounds exhibited extensive anti-cancer activities against the proliferation of various human cancer cell lines. In the current study, a systematic ligand based structural optimization led to new analogs that significantly inhibited the growth of a panel of breast cancer cell lines. Furthermore, the most potent compounds were examined with tubulin polymerization assay and Hsp27 chaperone activity assay. The compounds showed potent tubulin polymerization inhibition but no Hsp27 inhibitory effect. The structural optimization dissected …
Development And Validation Of Lc–Ms/Ms Method For Quantitative Determination Of (−)-Securinine In Mouse Plasma, Simuli L. Wabuyele, David Wald, Yan Xu
Development And Validation Of Lc–Ms/Ms Method For Quantitative Determination Of (−)-Securinine In Mouse Plasma, Simuli L. Wabuyele, David Wald, Yan Xu
Chemistry Faculty Publications
(−)-Securinine (SE) is a major alkaloid found in plant Securinega suffruticosa, which has a wide range of pharmacological activities including anticancer, anti-parasitic and central nervous system stimulating effects, etc. To aid the pharmacological study of SE, we developed an LC–MS/MS method for quantitative determination of SE in mouse plasma. In this method, plasma samples were first prepared with salting-out assisted liquid–liquid extraction using cold acetonitrile (−20 °C) and 2.00 M ammonium acetate. Separation of SE and the internal standard (IS) from sample matrix was achieved on a Gemini Nx C18 column using 40% acetonitrile and 60% 10.0 mM ammonium acetate …
Mechanistic Heterogeneity In Site Recognition By The Structurally Homologous Dna-Binding Domains Of The Ets-Family Transcription Factors Ets-1 And Pu.1, Shuo Wang, Miles H. Linde, Manoj Munde, Victor W. Carvalho, W. David Wilson, Gregory M. K. Poon
Mechanistic Heterogeneity In Site Recognition By The Structurally Homologous Dna-Binding Domains Of The Ets-Family Transcription Factors Ets-1 And Pu.1, Shuo Wang, Miles H. Linde, Manoj Munde, Victor W. Carvalho, W. David Wilson, Gregory M. K. Poon
Chemistry Faculty Publications
ETS-family transcription factors regulate diverse genes through binding at cognate DNA sites that overlap substantially in sequence. The DNA-binding domains of ETS proteins (ETS domains) are highly conserved structurally, yet share limited amino acid homology. To define the mechanistic implications of sequence diversity within the ETS family, we characterized the thermodynamics and kinetics of DNA site recognition by the ETS domains of Ets-1 and PU.1, which represent the extremes in amino acid divergence among ETS proteins. Even though the two ETS domains bind their optimal sites with similar affinities under physiologic conditions, their nature of site recognition differs strikingly in …
Prototype Nerve-Specific Near-Infrared Fluorophores, Min Ho Park, Hoon Hyun, Yoshitomo Ashitate, Hideyuki Wada, Gwangli Park, Jeong Heon Lee, Costyl Njiojob, Maged Henary, John V. Frangioni, Hak Soo Choi
Prototype Nerve-Specific Near-Infrared Fluorophores, Min Ho Park, Hoon Hyun, Yoshitomo Ashitate, Hideyuki Wada, Gwangli Park, Jeong Heon Lee, Costyl Njiojob, Maged Henary, John V. Frangioni, Hak Soo Choi
Chemistry Faculty Publications
Nerve preservation is an important issue during most surgery because accidental transection or injury results in significant morbidity, including numbness, pain, weakness, or paralysis. Currently, nerves are still identified only by gross appearance and anatomical location during surgery, without intraoperative image guidance. Near-infrared (NIR) fluorescent light, in the wavelength range of 650-900 nm, has the potential to provide high-resolution, high-sensitivity, and real-time avoidance of nerve damage, but only if nerve-specific NIR fluorophores can be developed. In this study, we evaluated a series of Oxazine derivatives to highlight various peripheral nerve structures in small and large animals. Among the targeted fluorophores, …
Co2 Reduction Under Periodic Illumination Of Zns, Ruixin Zhou, Marcelo I. Guzman
Co2 Reduction Under Periodic Illumination Of Zns, Ruixin Zhou, Marcelo I. Guzman
Chemistry Faculty Publications
The photoreduction of CO2 to formate (HCOO–) in sphalerite (ZnS) aqueous suspensions is systematically studied in the presence of Na2S hole scavenger. A series of cut-on filters at λcut-on ≥ 280, 295, 305, 320, and 400 nm are used to measure the reaction rate of formate production. The dependence of the measured reaction rates on λcut-on indicates that a wavelength of λ = 345 nm is associated with the actual bandgap of the semiconductor nanocrystallites suspended in water. The results from apparent quantum yield measurements during periodic illumination experiments suggest that (1) valence-band …
Reclaiming The Efficacy Of Β-Lactam–Β-Lactamase Inhibitor Combinations: Avibactam Restores The Susceptibility Of Cmy-2-Producing Escherichia Coli To Ceftazidime, Krisztina M. Papp-Wallace, Marisa L. Winkler, Julian A. Gatta, Magdalena A. Taracila, Sujatha Chilakala, Yan Xu, J. Kristie Johnson, Robert A. Bonomo
Reclaiming The Efficacy Of Β-Lactam–Β-Lactamase Inhibitor Combinations: Avibactam Restores The Susceptibility Of Cmy-2-Producing Escherichia Coli To Ceftazidime, Krisztina M. Papp-Wallace, Marisa L. Winkler, Julian A. Gatta, Magdalena A. Taracila, Sujatha Chilakala, Yan Xu, J. Kristie Johnson, Robert A. Bonomo
Chemistry Faculty Publications
CMY-2 is a plasmid-encoded Ambler class C cephalosporinase that is widely disseminated in Enterobacteriaceae and is responsible for expanded-spectrum cephalosporin resistance. As a result of resistance to both ceftazidime and β-lactamase inhibitors in strains carrying blaCMY, novel β-lactam–β-lactamase inhibitor combinations are sought to combat this significant threat to β-lactam therapy. Avibactam is a bridged diazabicyclo [3.2.1]octanone non-β-lactam β-lactamase inhibitor in clinical development that reversibly inactivates serine β-lactamases. To define the spectrum of activity of ceftazidime-avibactam, we tested the susceptibilities of Escherichia coli clinical isolates that carry blaCMY-2 or blaCMY-69 and investigated the inactivation kinetics of CMY-2. Our analysis showed that …
Effect Of Ca2+ On The Steady-State And Time-Resolved Emission Properties Of The Genetically Encoded Fluorescent Sensor Catcher, You Zhou, Kyril M. Solntsev, Florence Reddish, Shen Tang, Jenny J. Yang
Effect Of Ca2+ On The Steady-State And Time-Resolved Emission Properties Of The Genetically Encoded Fluorescent Sensor Catcher, You Zhou, Kyril M. Solntsev, Florence Reddish, Shen Tang, Jenny J. Yang
Chemistry Faculty Publications
We previously designed a calcium sensor CatchER (a GFP-based Calcium sensor for detecting high concentrations in the high calcium concentration environment such as ER) with a capability for monitoring calcium ion responses in various types of cells. Calcium binding to CatchER induces the ratiometric changes in the absorption spectra, as well as an increase in fluorescence emission at 510 nm upon excitation at both 395 and 488 nm. Here, we have applied the combination of the steady-state and time-resolved optical methods and Hydrogen/Deuterium isotope exchange to understand the origin of such calcium-induced optical property changes of CatchER. We first demonstrated …
A Small Molecule That Binds And Inhibits The Etv1 Transcription Factor Oncoprotein, Marius C. Pop, Nicolas Stransky, Colin W. Garvie, Jean-Philippe Theurillat, Emily C. Hartman, Timothy A. Lewis, Cheng Zhong, Elizabeth K. Culyba, Fallon Lin, Douglas S. Daniels, Raymond Pagliarini, Lucienne Ronco, Angela N. Koehler, Levi A. Garraway
A Small Molecule That Binds And Inhibits The Etv1 Transcription Factor Oncoprotein, Marius C. Pop, Nicolas Stransky, Colin W. Garvie, Jean-Philippe Theurillat, Emily C. Hartman, Timothy A. Lewis, Cheng Zhong, Elizabeth K. Culyba, Fallon Lin, Douglas S. Daniels, Raymond Pagliarini, Lucienne Ronco, Angela N. Koehler, Levi A. Garraway
Chemistry Faculty Publications
Members of the ETS transcription factor family have been implicated in several cancers, where they are often dysregulated by genomic derangement. ETS variant 1 (ETV1) is an ETS factor gene that undergoes chromosomal translocation in prostate cancers and Ewing's sarcomas, amplification in melanomas, and lineage dysregulation in gastrointestinal stromal tumors. Pharmacologic perturbation of ETV1 would be appealing in these cancers; however, oncogenic transcription factors are often deemed “undruggable” by conventional methods. Here, we used small-molecule microarray (SMM) screens to identify and characterize drug-like compounds that modulate the biological function of ETV1. We identified the 1,3,5-triazine small molecule BRD32048 as a …
Site-Specific Nitration Of Apolipoprotein A-I At Tyrosine 166 Is Both Abundant Within Human Atherosclerotic Plaque And Dysfunctional, Joseph A. Didonato, Kulwant Aulak, Ying Huang, Matthew A. Wagner, Gary Gerstenecker, Celalettin Topbas, Valentin Gogonea, Anthony J. Didonato, W.H. Wilson Tang, Ryan A. Mehl, Paul L. Fox, Edward F. Plow, Jonathan D. Smith, Edward A. Fisher, Stanley L. Hazen
Site-Specific Nitration Of Apolipoprotein A-I At Tyrosine 166 Is Both Abundant Within Human Atherosclerotic Plaque And Dysfunctional, Joseph A. Didonato, Kulwant Aulak, Ying Huang, Matthew A. Wagner, Gary Gerstenecker, Celalettin Topbas, Valentin Gogonea, Anthony J. Didonato, W.H. Wilson Tang, Ryan A. Mehl, Paul L. Fox, Edward F. Plow, Jonathan D. Smith, Edward A. Fisher, Stanley L. Hazen
Chemistry Faculty Publications
We reported previously that apolipoprotein A-I (apoA-I) is oxidatively modified in the artery wall at tyrosine 166 (Tyr166), serving as a preferred site for post-translational modification through nitration. Recent studies, however, question the extent and functional importance of apoA-I Tyr166 nitration based upon studies of HDL-like particles recovered from atherosclerotic lesions. We developed a monoclonal antibody (mAb 4G11.2) that recognizes, in both free and HDL-bound forms, apoA-I harboring a 3-nitrotyrosine at position 166 apoA-I (NO2-Tyr166-apoA-I) to investigate the presence, distribution, and function of this modified apoA-I form in atherosclerotic and normal artery wall. We also developed recombinant apoA-I with site-specific …
Designing Conical Intersections For Light-Driven Single Molecule Rotary Motors: From Precessional To Axial Motion, Michael Filatov, Massimo Olivucci
Designing Conical Intersections For Light-Driven Single Molecule Rotary Motors: From Precessional To Axial Motion, Michael Filatov, Massimo Olivucci
Chemistry Faculty Publications
In the past, the design of light-driven single molecule rotary motors has been mainly guided by the modification of their ground-state conformational properties. Further progress in this field is thus likely to be achieved through a detailed understanding of light-induced dynamics of the system and the ways of modulating it by introducing chemical modifications. In the present theoretical work, the analysis of model organic chromophores and synthesized rotary motors is used for rationalizing the effect of electron-withdrawing heteroatoms (such as a cationic nitrogen) on the topography and branching plane of mechanistically relevant conical intersections. Such an analysis reveals how the …
Simultaneous Mapping Of Pan And Sentinel Lymph Nodes For Real-Time Image-Guided Surgery, Yoshitomo Ashitate, Hoon Hyun, Soon Hee Kim, Jeong Heon Lee, Maged Henary, John V. Frangioni, Hak Soo Choi
Simultaneous Mapping Of Pan And Sentinel Lymph Nodes For Real-Time Image-Guided Surgery, Yoshitomo Ashitate, Hoon Hyun, Soon Hee Kim, Jeong Heon Lee, Maged Henary, John V. Frangioni, Hak Soo Choi
Chemistry Faculty Publications
The resection of regional lymph nodes in the basin of a primary tumor is of paramount importance in surgical oncology. Although sentinel lymph node mapping is now the standard of care in breast cancer and melanoma, over 20% of patients require a completion lymphadenectomy. Yet, there is currently no technology available that can image all lymph nodes in the body in real time, or assess both the sentinel node and all nodes simultaneously. In this study, we report an optical fluorescence technology that is capable of simultaneous mapping of pan lymph nodes (PLNs) and sentinel lymph nodes (SLNs) in the …
Structures Of Darunavir-Resistant Hiv‑1 Protease Mutant Reveal Atypical Binding Of Darunavir To Wide Open Flaps, Ying Zhang, Yu-Chung E. Chang, John M. Louis, Yuan Fang Wang, Robert W. Harrison, Irene Weber
Structures Of Darunavir-Resistant Hiv‑1 Protease Mutant Reveal Atypical Binding Of Darunavir To Wide Open Flaps, Ying Zhang, Yu-Chung E. Chang, John M. Louis, Yuan Fang Wang, Robert W. Harrison, Irene Weber
Chemistry Faculty Publications
The molecular basis for high resistance to clinical inhibitors of HIV-1 protease (PR) was examined for the variant designated PRP51 that was selected for resistance to darunavir (DRV). High resolution crystal structures of PRP51 with the active site D25N mutation revealed a ligand-free form and an inhibitor-bound form showing a unique binding site and orientation for DRV. This inactivating mutation is known to increase the dimer dissociation constant and decrease DRV affinity of PR. The PRP51‑D25N dimers were in the open conformation with widely separated flaps, as reported for other highly resistant variants. PRP51‑D25N dimer bound two DRV molecules and …
Lead Optimization Of Hmba To Develop Potent Hexim1 Inducers, Bo Zhong, Rati Lama, Wannarasmi Ketchart, Monica M. Montano, Bin Su
Lead Optimization Of Hmba To Develop Potent Hexim1 Inducers, Bo Zhong, Rati Lama, Wannarasmi Ketchart, Monica M. Montano, Bin Su
Chemistry Faculty Publications
The potency of a series of Hexamethylene bis-acetamide (HMBA) derivatives inducing Hexamethylene bis-acetamide inducible protein 1 (HEXIM1) was determined in LNCaP prostate cancer cells. Several compounds with unsymmetrical structures showed significantly improved activity. Distinct from HMBA, these analogs have increased hydrophobicity and can improve the short half-life of HMBA, which is one of the factors that have limited the application of HMBA in clinics. The unsymmetrical scaffolds of the new analogs provide the basis for further lead optimization of the compounds using combinatorial chemistry strategy.
Selective Anion Sensing By Chiral Macrocyclic Receptors With Multiple Hydrogen-Bonding Sites, Tadashi Ema, Keiichi Okuda, Sagiri Watanabe, Takayuki Yamasaki, Tsuyoshi Minami, Nina A. Esipenko, Pavel Anzenbacher Jr.
Selective Anion Sensing By Chiral Macrocyclic Receptors With Multiple Hydrogen-Bonding Sites, Tadashi Ema, Keiichi Okuda, Sagiri Watanabe, Takayuki Yamasaki, Tsuyoshi Minami, Nina A. Esipenko, Pavel Anzenbacher Jr.
Chemistry Faculty Publications
Chiral macrocycles featuring sulfonamide and/or amide groups as anion-binding sites were synthesized. X-ray crystal structures and DFT calculations have shown that they adopt different conformations that may lead to unique binding behavior. Indeed, various anions could be sensed by their colorimetric and/or fluorescence signal output. The chiral macrocycles showed chiral recognition for chiral anions. Furthermore, a multisensor array with two or four chiral receptors discriminated seven phosphate anions (AMP, ADP, ATP, CMP, GMP, Pi, and PPi) with 100% classification accuracy.
Nonproteolytic Roles Of 19s Atpases In Transcription Of Ciitapiv Genes, Nagini Maganti, Tomika Moody, Agnieszka D. Truax, Meghna Thakkar, Alexander M. Spring, Markus W. Germann, Susanna F. Greer
Nonproteolytic Roles Of 19s Atpases In Transcription Of Ciitapiv Genes, Nagini Maganti, Tomika Moody, Agnieszka D. Truax, Meghna Thakkar, Alexander M. Spring, Markus W. Germann, Susanna F. Greer
Chemistry Faculty Publications
Accumulating evidence shows the 26S proteasome is involved in the regulation of gene expression. We and others have demonstrated that proteasome components bind to sites of gene transcription, regulate covalent modifications to histones, and are involved in the assembly of activator complexes in mammalian cells. The mechanisms by which the proteasome influences transcription remain unclear, although prior observations suggest both proteolytic and non-proteolytic activities. Here, we define novel, non-proteolytic, roles for each of the three 19S heterodimers, represented by the 19S ATPases Sug1, S7, and S6a, in mammalian gene expression using the inflammatory gene CIITApIV. These 19S ATPases are recruited …
Drug Analogs Of Cox-2 Selective Inhibitors Lumiracoxib And Valdecoxib Derived From In Silico Search And Optimization, Ronald Bartzatt
Drug Analogs Of Cox-2 Selective Inhibitors Lumiracoxib And Valdecoxib Derived From In Silico Search And Optimization, Ronald Bartzatt
Chemistry Faculty Publications
The medicinal activity of COX-2 inhibitors are sufficiently beneficial to urge the search for new drug designs. This study presents 16 analogs of lumiracoxib and 10 analogs to valdecoxib having properties suitable as COX-2 inhibitors. For lumiracoxib analogs the mean Log P, polar surface area, and formula weight are 3.00, 70.46 A2, and 276.60, respectively. For valdecoxib analogs the mean Log P, polar surface area, and formula weight are 3.65, 68.46 A2, and 322.32, respectively. Grubb’s test analysis of seven properties for seven known COX-2 selective inhibitors and those of 26 analog compounds indicated no outliers. The unpaired t-test compared …
Multi-Technique Quantitative Analysis And Socioeconomic Considerations Of Lead, Cadmium, And Arsenic In Children's Toys And Toy Jewelry, Margot M. Hillyer, Lauren E. Finch, Alisha S. Cerel, Jonathan D. Dattelbaum, Michael C. Leopold
Multi-Technique Quantitative Analysis And Socioeconomic Considerations Of Lead, Cadmium, And Arsenic In Children's Toys And Toy Jewelry, Margot M. Hillyer, Lauren E. Finch, Alisha S. Cerel, Jonathan D. Dattelbaum, Michael C. Leopold
Chemistry Faculty Publications
A wide spectrum and large number of children’s toys and toy jewelry items were purchased from both bargain and retail vendors and analyzed for arsenic, cadmium, and lead metal content using multiple analytical techniques, including flame and furnace atomic absorption spectroscopy as well as X-ray fluorescence spectroscopy. Particularly dangerous for young children, metal concentrations in toys/toy jewelry were assessed for compliance with current Consumer Safety Product Commission (CPSC) regulations (F963-11). A conservative metric involving multiple analytical techniques was used to categorize compliance: one technique confirmation of metal in excess of CPSC limits indicated a “suspect” item while confirmation on two …
Functional Relevance Of Acrb Trimerization In Pump Assembly And Substrate Binding, Wei Lu, Meng Zhong, Qian Chai, Zhaoshuai Wang, Linliang Yu, Yinan Wei
Functional Relevance Of Acrb Trimerization In Pump Assembly And Substrate Binding, Wei Lu, Meng Zhong, Qian Chai, Zhaoshuai Wang, Linliang Yu, Yinan Wei
Chemistry Faculty Publications
AcrB is a multidrug transporter in the inner membrane of Escherichia coli. It is an obligate homotrimer and forms a tripartite efflux complex with AcrA and TolC. AcrB is the engine of the efflux machinery and determines substrate specificity. Active efflux depends on several functional features including proton translocation across the inner membrane through a proton relay pathway in the transmembrane domain of AcrB; substrate binding and migration through the substrate translocation pathway; the interaction of AcrB with AcrA and TolC; and the formation of AcrB homotrimer. Here we investigated two aspects of the inter-correlation between these functional features, …
A Review Of Air–Ice Chemical And Physical Interactions (Aici): Liquids, Quasi-Liquids, And Solids In Snow, T. Bartels-Rausch, H. -W. Jacobi, T. F. Kahan, J. L. Thomas, E. S. Thomson, J. P. D. Abbatt, M. Ammann, J. R. Blackford, H. Bluhm, C. Boxe, F. Domine, M. M. Frey, I. Gladich, M. I. Guzmán, D. Heger, Th. Huthwelker, P. Klán, W. F. Kuhs, M. H. Kuo, S. Maus, S. G. Moussa, V. F. Mcneill, J. T. Newberg, J. B. C. Pettersson, M. Roeselová, J. R. Sodeau
A Review Of Air–Ice Chemical And Physical Interactions (Aici): Liquids, Quasi-Liquids, And Solids In Snow, T. Bartels-Rausch, H. -W. Jacobi, T. F. Kahan, J. L. Thomas, E. S. Thomson, J. P. D. Abbatt, M. Ammann, J. R. Blackford, H. Bluhm, C. Boxe, F. Domine, M. M. Frey, I. Gladich, M. I. Guzmán, D. Heger, Th. Huthwelker, P. Klán, W. F. Kuhs, M. H. Kuo, S. Maus, S. G. Moussa, V. F. Mcneill, J. T. Newberg, J. B. C. Pettersson, M. Roeselová, J. R. Sodeau
Chemistry Faculty Publications
Snow in the environment acts as a host to rich chemistry and provides a matrix for physical exchange of contaminants within the ecosystem. The goal of this review is to summarise the current state of knowledge of physical processes and chemical reactivity in surface snow with relevance to polar regions. It focuses on a description of impurities in distinct compartments present in surface snow, such as snow crystals, grain boundaries, crystal surfaces, and liquid parts. It emphasises the microscopic description of the ice surface and its link with the environment. Distinct differences between the disordered air–ice interface, often termed quasi-liquid …
Kinetics And Structures On The Molecular Path To The Quadruplex Form Of The Human Telomere, W. David Wilson, Ananya Paul
Kinetics And Structures On The Molecular Path To The Quadruplex Form Of The Human Telomere, W. David Wilson, Ananya Paul
Chemistry Faculty Publications
No abstract provided.
The Unusual Monomer Recognition Of Guanine-Containing Mixed Sequence Dna By A Dithiophene Heterocyclic Diamidine, Manoj Munde, Arvind Kumar, Paul Peixoto, Sabine Depauw, Mohamed A. Ismail, Abdelbasset A. Farahat, Ananya Paul, Martial V. Say, Marie-HéLèNe David-Cordonnier, David Boykin, W. David Wilson
The Unusual Monomer Recognition Of Guanine-Containing Mixed Sequence Dna By A Dithiophene Heterocyclic Diamidine, Manoj Munde, Arvind Kumar, Paul Peixoto, Sabine Depauw, Mohamed A. Ismail, Abdelbasset A. Farahat, Ananya Paul, Martial V. Say, Marie-HéLèNe David-Cordonnier, David Boykin, W. David Wilson
Chemistry Faculty Publications
DB1255 is a symmetrical diamidinophenyl-dithiophene that exhibits cellular activity by binding to DNA and inhibiting binding of ERG, an ETS family transcription factor that is commonly overexpressed or translocated in leukemia and prostate cancer [Nhili, R., Peixoto, P., Depauw, S., Flajollet, S., Dezitter, X., Munde, M. M., Ismail, M. A., Kumar, A., Farahat, A. A., Stephens, C. E., Duterque-Coquillaud, M., Wilson, W. D., Boykin, D. W., and David-Cordonnier, M. H. (2013) Nucleic Acids Res. 41, 125−138]. Because transcription factor inhibition is complex but is an attractive area for anticancer and antiparasitic drug development, we have evaluated the DNA interactions of …
The Unusual Monomer Recognition Of Guanine-Containing Mixed Sequence Dna By A Dithiophene Heterocyclic Diamidine, Manoj Munde, Arvind Kumar, Paul Peixoto, Sabine Depauw, Mohamed A. Ismail, Abdelbasset A. Farahat, Ananya Paul, Martial V. Say, Marie-HéLèNe David-Cordonnier, David Boykin, W. David Wilson
The Unusual Monomer Recognition Of Guanine-Containing Mixed Sequence Dna By A Dithiophene Heterocyclic Diamidine, Manoj Munde, Arvind Kumar, Paul Peixoto, Sabine Depauw, Mohamed A. Ismail, Abdelbasset A. Farahat, Ananya Paul, Martial V. Say, Marie-HéLèNe David-Cordonnier, David Boykin, W. David Wilson
Chemistry Faculty Publications
DB1255 is a symmetrical diamidinophenyl-dithiophene that exhibits cellular activity by binding to DNA and inhibiting binding of ERG, an ETS family transcription factor that is commonly overexpressed or translocated in leukemia and prostate cancer [Nhili, R., Peixoto, P., Depauw, S., Flajollet, S., Dezitter, X., Munde, M. M., Ismail, M. A., Kumar, A., Farahat, A. A., Stephens, C. E., Duterque-Coquillaud, M., Wilson, W. D., Boykin, D. W., and David-Cordonnier, M. H. (2013) Nucleic Acids Res. 41, 125−138]. Because transcription factor inhibition is complex but is an attractive area for anticancer and antiparasitic drug development, we have evaluated the DNA interactions of …
Dna Binding Polyamides And The Importance Of Dna Recognition In Their Use As Gene-Specific And Antiviral Agents, Kevin J. Koeller, G Davis Harris, Karl Aston, Gaofei He, Carlos H. Castaneda, Melissa A. Thornton, Terri G. Edwards, Shuo Wang, Rupesh Nanjunda, W. David Wilson, Chris Fisher, James K. Bashkin
Dna Binding Polyamides And The Importance Of Dna Recognition In Their Use As Gene-Specific And Antiviral Agents, Kevin J. Koeller, G Davis Harris, Karl Aston, Gaofei He, Carlos H. Castaneda, Melissa A. Thornton, Terri G. Edwards, Shuo Wang, Rupesh Nanjunda, W. David Wilson, Chris Fisher, James K. Bashkin
Chemistry Faculty Publications
There is a long history for the bioorganic and biomedical use of N-methyl-pyrrole-derived polyamides (PAs) that are higher homologs of natural products such as distamycin A and netropsin. This work has been pursued by many groups, with the Dervan and Sugiyama groups responsible for many breakthroughs. We have studied PAs since about 1999, partly in industry and partly in academia. Early in this program, we reported methods to control cellular uptake of polyamides in cancer cell lines and other cells likely to have multidrug resistance efflux pumps induced. We went on to discover antiviral polyamides active against HPV31, where SAR …
An Abundant Dysfunctional Apolipoprotein A1 In Human Atheroma, Ying Huang, Joseph A. Didonato, Bruce S. Levison, Dave Schmitt, Lin Li, Yuping Wu, Jennifer Buffa, Timothy Kim, Gary S. Gerstenecker, Xiaodong Gu, Chandra S. Kadiyala, Zeneng Wang, Miranda K. Culley, Jennie E. Hazen, Anthony J. Didonato, Xiaoming Fu, Stela Z. Berisha, Daoquan Peng, Truc T. Nguyen, Shaohong Liang, Chia-Chi Chuang, Leslie Cho, Edward F. Plow, Paul L. Fox, Valentin Gogonea, W.H. Wilson Tang, John S. Parks, Edward A. Fisher, Jonathan D. Smith, Stanley L. Hazen
An Abundant Dysfunctional Apolipoprotein A1 In Human Atheroma, Ying Huang, Joseph A. Didonato, Bruce S. Levison, Dave Schmitt, Lin Li, Yuping Wu, Jennifer Buffa, Timothy Kim, Gary S. Gerstenecker, Xiaodong Gu, Chandra S. Kadiyala, Zeneng Wang, Miranda K. Culley, Jennie E. Hazen, Anthony J. Didonato, Xiaoming Fu, Stela Z. Berisha, Daoquan Peng, Truc T. Nguyen, Shaohong Liang, Chia-Chi Chuang, Leslie Cho, Edward F. Plow, Paul L. Fox, Valentin Gogonea, W.H. Wilson Tang, John S. Parks, Edward A. Fisher, Jonathan D. Smith, Stanley L. Hazen
Chemistry Faculty Publications
Recent studies have indicated that high-density lipoproteins (HDLs) and their major structural protein, apolipoprotein A1 (apoA1), recovered from human atheroma are dysfunctional and are extensively oxidized by myeloperoxidase (MPO). In vitro oxidation of either apoA1 or HDL particles by MPO impairs their cholesterol acceptor function. Here, using phage display affinity maturation, we developed a high-affinity monoclonal antibody that specifically recognizes both apoA1 and HDL that have been modified by the MPO-H2O2-Cl− system. An oxindolyl alanine (2-OH-Trp) moiety at Trp72 of apoA1 is the immunogenic epitope. Mutagenesis studies confirmed a critical role for apoA1 Trp72 in MPO-mediated inhibition of the ATP-binding …
Detection And Assay Of Riboflavin (Vitamin B2) Utilizing Uv/Vis Spectrophotometer And Citric Acid Buffer, Ronald Bartzatt, Michelle Lee Follis
Detection And Assay Of Riboflavin (Vitamin B2) Utilizing Uv/Vis Spectrophotometer And Citric Acid Buffer, Ronald Bartzatt, Michelle Lee Follis
Chemistry Faculty Publications
Aims: Riboflavin is a B vitamin that is required for a wide variety of cellular processes. The absorbance spectrum of riboflavin was determined at different pH utilizing several buffers. The buffer at pH demonstrating table absorbance peaks with high numerical values of molar absorptivity is followed by accurate and sensitive assay of riboflavin by spectrophotometer. Study Design: The absorbance spectrum of riboflavin is determined in an aqueous buffer at various pH values. After identifying the absorbance peaks providing maximal molar absorptivity the assay of riboflavin in the identical buffer was undertaken. Place and Duration of Study: Department of Chemistry, Durham …
Bsa–Boronic Acid Conjugate As Lectin Mimetics, Satya Nandana Narla, Poornima Pinnamaneni, Huan Nie, Yu Li, Xue-Long Sun
Bsa–Boronic Acid Conjugate As Lectin Mimetics, Satya Nandana Narla, Poornima Pinnamaneni, Huan Nie, Yu Li, Xue-Long Sun
Chemistry Faculty Publications
We report bovine serum albumin (BSA)–boronic acid (BA) conjugates as lectin mimetics and their glyco-capturing capacity. The BSA–BA conjugates were synthesized by amidation of carboxylic acid groups in BSA with aminophenyl boronic acid in the presence of EDC, and were characterized by Alizarin Red S (ARS) assay and SDS–PAGE gel. The BSA–BA conjugates were immobilized onto maleimide-functionalized silica beads and their sugar capturing capacity and specificity were confirmed by ARS displacement assay. Further, surface plasmon resonance (SPR) analysis of the glyco-capturing activity of the BSA–BA conjugates was conducted by immobilizing BSA–BA onto SPR gold chip. Overall, we demonstrated a BSA–BA-based …
Rat Hippocampal Responses Up To 90 Days After A Single Nanoceria Dose Extends A Hierarchical Oxidative Stress Model For Nanoparticle Toxicity, Sarita S. Hardas, Rukhsana Sultana, Govind Warrier, Mo Dan, Peng Wu, Eric A. Grulke, Michael T. Tseng, Jason M. Unrine, Uschi M. Graham, Robert A. Yokel, D. Allan Butterfield
Rat Hippocampal Responses Up To 90 Days After A Single Nanoceria Dose Extends A Hierarchical Oxidative Stress Model For Nanoparticle Toxicity, Sarita S. Hardas, Rukhsana Sultana, Govind Warrier, Mo Dan, Peng Wu, Eric A. Grulke, Michael T. Tseng, Jason M. Unrine, Uschi M. Graham, Robert A. Yokel, D. Allan Butterfield
Chemistry Faculty Publications
Ceria engineered nanomaterials (ENMs) have very promising commercial and therapeutic applications. Few reports address the effects of nanoceria in intact mammals, let alone long term exposure. This knowledge is essential to understand potential therapeutic applications of nanoceria in relation to its hazard assessment. The current study elucidates oxidative stress responses in the rat hippocampus 1 and 20 h, and 1, 7, 30 and 90 days following a single systemic infusion of 30 nm nanoceria. The results are incorporated into a previously described hierarchical oxidative stress (HOS) model. During the 1-20 h period, increases of the GSSG: GSH ratio and cytoprotective …
Nonexistent Compounds As A Guide To Innovation, Dean F. Martin, Barbara B. Martin
Nonexistent Compounds As A Guide To Innovation, Dean F. Martin, Barbara B. Martin
Chemistry Faculty Publications
A study of nonexistent compounds can be a useful exercise in gaining insight into the factors that can inhibit innovation. Several reasons are suggested: lack of financial support, disinterest in preparing compounds that lack evident utility, notable synthetic challenges with inadequate rewards, inhibition by well-established contemporary knowledge, and invalid interpolations.
Effects Of Oxidation On Protein-Nanoparticle Interactions, Valdez R. Rahming, Md. Abul Fazal
Effects Of Oxidation On Protein-Nanoparticle Interactions, Valdez R. Rahming, Md. Abul Fazal
Chemistry Faculty Publications
Aims: Upon entrance into the blood stream most nanoparticles bind to an array of proteins forming a “protein corona”. Fibrinogen is the second most abundant blood protein and has been reported to bind to a variety of nanoparticles including metal oxides, polymeric nanoparticles and carbon nanotubes.
Study Design: Study the effects of oxidation on the binding interactions between human serum fibrinogen and magnetic iron (III) oxide nanoparticles.
Place and Duration of Study: Department of Chemistry, College of St. Benedict, 37 South College Avenue, St. Joseph, MN 56374, U.S.A., between June 2011 and May 2012.
Methodology: Spectroscopic techniques (UV-Vis, IR, fluorescence, …
Quantification Of Free Sialic Acid In Human Plasma Through A Robust Quinoxalinone Derivatization And Lc–Ms/Ms Using Isotope-Labeled Standard Calibration, Dan Wang, Xiang Zhou, Lin Wang, Sihe Wang, Xue-Long Sun
Quantification Of Free Sialic Acid In Human Plasma Through A Robust Quinoxalinone Derivatization And Lc–Ms/Ms Using Isotope-Labeled Standard Calibration, Dan Wang, Xiang Zhou, Lin Wang, Sihe Wang, Xue-Long Sun
Chemistry Faculty Publications
We report an accurate quantification of free sialic acid (SA) in human plasma using LC–MS/MS method with isotope-labeled standard calibration (ILSC) and robust derivatization. Specifically, derivatization of SA with a stable and inexpensive 3,4-diaminotoluene (DAT) provides a stable product of SA with high MS response, proving a convenient and cost-effective LC–MS/MS analysis of free SA. In addition, the use of 13C3-SA as calibration standard ensured the accuracy for the measurement. This assay used ultra high performance liquid chromatography (UHPLC) for separation of native/labeled SA and IS from matrix interference, and employed mass spectrometry in multiple reaction monitoring …