Physical Sciences and Mathematics Commons^™

Synthesis And Characterization Of Inosamines, Sing Raj Gurung

Masters Theses

This project is concerned with the preparation of scyllo-inosarnine hydrochloride 6, a biologically active compound, which bears six successive stereogenic centers. The synthesis of this compound would help elucidate the involvement of inosamines in biological nitrogen fixation. myo-Inositol served as the starting material to synthesize target compound 6. Regioselective protection and deprotection of myo-inositol provided azide 43 as key intermediate. In the final steps deprotection of five benzyl groups and reduction of azido group was achieved by catalytic hydrogenation. scyllo-Inosamine hydrochloride 6 was fully characterized by m.p., ¹H NMR, ¹³C NMR, IR, EA and via …

Expression And Characterization Of Hyperthermophilic Aqujfex Pyrophilus Flagellin Protein In Mesophilic Bacterial Systems, Srivani V. Mukkamala

Masters Theses

The structural protein flagellin, FlaA derived from hyperthermophilic Aquifex pyrophilus, a rod shaped eubacterial species that grows near hydrothermal vents at an optimum growth temperature of 85 °C forms elongated thermostable flagella nanotubes. FlaA was successfully over-expressed for the first time using pET28c plasmid in E. coli BL21 cells at 37 °C. The protein was isolated from inclusion bodies that were solubilized by alkaline pH, refolded by dialysis and purified by chromatographic techniques. The FlaA was characterized by fluorescence, light scattering and circular dichroism. The FlaA was not in the correct folded form to form flagella as efforts to …

Coordination Environment: A New Approach To The Sensing Of Lead (Ii) Itself, Kristina Marie Nespechal

Masters Theses

Lead(II) is a silent poison that has plagued society since 3800 B. C. The average intake of lead in the United States alone is 50 μg per day, which is approximately 100-1000 times greater than that of prehistoric lead levels. Regardless of the banning of lead in paints and gasoline, lead poisoning prevails as a major environmental health risk. Science has the ability to address the concern with this environmental contaminant. The future production of an economical and reliable test kit for lead(II) relies on the luminescence of the heavy metal itself.

The absorption and emission spectra for a series …