Physical Sciences and Mathematics Commons^™

Expression And Characterization Of Hyperthermophilic Aqujfex Pyrophilus Flagellin Protein In Mesophilic Bacterial Systems, Srivani V. Mukkamala

Masters Theses

The structural protein flagellin, FlaA derived from hyperthermophilic Aquifex pyrophilus, a rod shaped eubacterial species that grows near hydrothermal vents at an optimum growth temperature of 85 °C forms elongated thermostable flagella nanotubes. FlaA was successfully over-expressed for the first time using pET28c plasmid in E. coli BL21 cells at 37 °C. The protein was isolated from inclusion bodies that were solubilized by alkaline pH, refolded by dialysis and purified by chromatographic techniques. The FlaA was characterized by fluorescence, light scattering and circular dichroism. The FlaA was not in the correct folded form to form flagella as efforts to …

Synthesis And Characterization Of Inosamines, Sing Raj Gurung

Masters Theses

This project is concerned with the preparation of scyllo-inosarnine hydrochloride 6, a biologically active compound, which bears six successive stereogenic centers. The synthesis of this compound would help elucidate the involvement of inosamines in biological nitrogen fixation. myo-Inositol served as the starting material to synthesize target compound 6. Regioselective protection and deprotection of myo-inositol provided azide 43 as key intermediate. In the final steps deprotection of five benzyl groups and reduction of azido group was achieved by catalytic hydrogenation. scyllo-Inosamine hydrochloride 6 was fully characterized by m.p., ¹H NMR, ¹³C NMR, IR, EA and via …

Applications Of Mass Spectrometry To Synthetic Copolymers, Elizabeth Angela Stewart

Masters Theses

Over the last decade, application of mass spectrometry has grown immensely especially in polymer characterization. The focus of this thesis is to assess and extend the applicability of matrix-assisted laser desorption ionization mass spectrometry (MALDI MS) and electrospray ionization mass spectrometry (ESI MS) in the analysis of synthetic copolymers.

Characterization of a non-ionic copolymer was attempted. The copolymer was styrene-co-maleic anhydride, which is in a class of copolymers that are used for commercial applications where low viscosity, high polarity and/or high reactivity are needed. MALDI-TOF MS was employed to obtain weight and number average molecular weight values (M_w and …

Development Of Novel Synthetic Methods Utilizing Organometallic Reagents And Total Synthesis Of Eupomatilone 2, Chunlan Chen

Masters Theses

This dissertation summarizes the development of novel synthetic methods involving organometallic reagents and their application in the total synthesis of the natural product eupomatilone 2. These newly discovered reactions include the palladium-catalyzed cross-coupling of allyl acetates with organosiloxanes and the diastereoselective synthesis of cis- and trans-a-methylene-y-lactones utilizing Baylis-Hillman adducts as precursors. Another important aspect of the research is the application of some of the methods in total synthesis of eupomatilone 2.

The new synthetic methodologies involving organometallic reagents developed in this dissertation are important transformations in modern organic synthesis. Mild reaction conditions, tolerance of various organic functional groups, and high …

An Investigation Into A Microwave-Enhanced Solvent-Free Mannich Condensation Reaction, Kristy Lynn Homburger

Masters Theses

Microwave irradiation has gained in popularity in recent years since it has been found to accelerate various chemical reactions. Microwaves are generally considered to be more environmentally friendly than traditional methods for activating chemical reactions. An investigation into a microwave-enhanced, solventless Mannich condensation was performed. The efficiency of a standard household microwave was compared to that of an industrial microwave, with the industrial microwave proving to be more effective. The advantages of using microwave irradiation for a Mannich condensation include milder reaction conditions, the absence of solvent, less waste, and shorter reaction times. The reaction between terminal alkynes, secondary amines, …

Free Radical Polymerization In Room Temperature Ionic Liquids, Xiao Ma

Masters Theses

No abstract provided.

Structural And Dynamical Studies Of Superacids And Superacidic Solutions Using Neutron And High Energy X-Ray Scattering, Jamie John Molaison

Masters Theses

The diffusive motions of the Brønsted superacid, hydrogen fluoride, have been studied using quasielastic neutron scattering. Neutron and high energy X-ray diffraction measurements on the Lewis superacid, antimony pentafluoride, are presented along with the results of three applied data models. Finally, structural information was obtained for a 9:1 FSO₃H:NH₃ solution using neutron diffraction with isotopic substitution and nuclear magnetic resonance.

Coordination Environment: A New Approach To The Sensing Of Lead (Ii) Itself, Kristina Marie Nespechal

Masters Theses

Lead(II) is a silent poison that has plagued society since 3800 B. C. The average intake of lead in the United States alone is 50 μg per day, which is approximately 100-1000 times greater than that of prehistoric lead levels. Regardless of the banning of lead in paints and gasoline, lead poisoning prevails as a major environmental health risk. Science has the ability to address the concern with this environmental contaminant. The future production of an economical and reliable test kit for lead(II) relies on the luminescence of the heavy metal itself.

The absorption and emission spectra for a series …

Novel Materials Used In Hydrogen Energy Economy, Hongliang Zhou

Masters Theses

The purpose of this study was to do some efforts in developing some novel polymer materials which could have the potential use in hydrogen pipelines and PEM fuel cell.

In the project of developing hydrogen pipelines, sulfonated PET, sulfonated PET/Clay and PET/Clay were produced by the method of solvent-mixing. Characterizations with TEM, SAXS were made to determine the exfoliation degree of nanocomposites. TGA was carried out to measure the thermal stability, and hydrogen permeability was also taken in order to measure the H2 gas barrier performance. It was found that the addition of clay into S-PET or PET could decrease …

Structural And Magnetic Investigations Of Cs2Agf4 And Its Doped Derivatives, Michelle Renee Dolgos

Masters Theses

This thesis describes the structural and magnetic properties of solid state silver fluorides such as Cs₂AgF₄ and its doped derivatives. A wide range of techniques such as X-ray and neutron diffraction, susceptibility measurements and inelastic neutron scattering experiments are used to characterize these materials. Silver fluorides are important given their strongly correlated magnetic behavior and the potential to discover a new class of high T_C materials. Few studies have been done on silver (II) fluorides as their characterization has been problematic due to their inherent air and water sensitivity. This study is the first to accurately …

Spectroscopic Investigation Of Metal-Substitution Of Cytochrome C, Deepthi Kandi

Masters Theses

No abstract provided.

The Study Of Carbon Acylation Of Highly Delocalized Enolates Using Catalytic Dmap On 3-Phenyl Benzofuranone Derivatives, Xiguang Zhao

Masters Theses

No abstract provided.