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University of Nebraska - Lincoln
Department of Chemistry: Dissertations, Theses, and Student Research
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Applications Of High Performance Affinity Chromatography With High Capacity Stationary Phases Made By Entrapment, John A. Vargas Badilla
Applications Of High Performance Affinity Chromatography With High Capacity Stationary Phases Made By Entrapment, John A. Vargas Badilla
Department of Chemistry: Dissertations, Theses, and Student Research
High performance affinity chromatography (HPAC) is a technique that uses a biologically-related agent such as a transport protein or an antibody as the stationary phase in an HPLC system. In recent years, HPAC has been shown to be a valuable bioanalytical tool for studying solute-protein interactions. Human serum albumin (HSA), the most abundant protein in blood (with concentrations of 35 to 50 mg/mL in serum), has been shown to interact with many drugs, affecting their transport, excretion and metabolism. A physical method for immobilizing proteins in HPAC supports has been optimized in this dissertation by using HSA as a model …