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Full-Text Articles in Physical Sciences and Mathematics
Optimization Of Protein Entrapment In Affinity Microcolumns Using Hydrazide-Activated Silica And Glycogen As A Capping Agent, John Vargas-Badilla, Saumen Poddar, Shiden Azaria, Chenhua Zhang, David S. Hage
Optimization Of Protein Entrapment In Affinity Microcolumns Using Hydrazide-Activated Silica And Glycogen As A Capping Agent, John Vargas-Badilla, Saumen Poddar, Shiden Azaria, Chenhua Zhang, David S. Hage
David Hage Publications
Several approaches were compared for the entrapment of proteins within hydrazide-activated silica for use in affinity microcolumns and high performance affinity chromatography. Human serum albumin (HSA) and concanavalin A (Con A) were used as model proteins for this work. Items considered in this study included the role played by the solution volume, amount of added protein, and use of slurry vs. on-column entrapment on the levels of solute retention and extent of protein immobilization that could be obtained by means of entrapment. The levels of retention and protein immobilization were evaluated by injecting warfarin or 4-methylumbellipheryl α-D-mannopyranoside as solutes with …
Applications Of High Performance Affinity Chromatography With High Capacity Stationary Phases Made By Entrapment, John A. Vargas Badilla
Applications Of High Performance Affinity Chromatography With High Capacity Stationary Phases Made By Entrapment, John A. Vargas Badilla
Department of Chemistry: Dissertations, Theses, and Student Research
High performance affinity chromatography (HPAC) is a technique that uses a biologically-related agent such as a transport protein or an antibody as the stationary phase in an HPLC system. In recent years, HPAC has been shown to be a valuable bioanalytical tool for studying solute-protein interactions. Human serum albumin (HSA), the most abundant protein in blood (with concentrations of 35 to 50 mg/mL in serum), has been shown to interact with many drugs, affecting their transport, excretion and metabolism. A physical method for immobilizing proteins in HPAC supports has been optimized in this dissertation by using HSA as a model …