Physical Sciences and Mathematics Commons^™

Adsorption Kinetics Of Bovine Serum Albumin To Strong Anion Exchange Adsorbents: Application Of The Pore Diffusion Model To Resins And Membranes, Sarah Catherine Jensen

Graduate Theses - Chemical Engineering

The replacement of traditional bead-based chromatography by membranes has gained recent interest in the bioseparations industry. However, membranes have drawbacks such as lower binding capacity that make them unrealistic for some applications. The goal of this work is to compare the adsorption kinetics of anion exchange bead-based resin and membranes both on a theoretical modeling level and experimental level. Bovine serum albumin (BSA) was adsorbed to Q Sepharose FastFlow resin and Sartobind Q membrane to obtain adsorption equilibrium characteristics and kinetic data. A numerical solution was used to fit the pore diffusion model to the data and estimate a pore …