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Articles 1 - 6 of 6
Full-Text Articles in Veterinary Preventive Medicine, Epidemiology, and Public Health
Wild Pig Removal Reduces Pathogenic Bacteria In Low-Order Streams, Sara A. Bolds, B. Graeme Lockaby, Latif Kalin, Stephen S. Ditchkoff, Mark D. Smith, Kurt Vercauteren
Wild Pig Removal Reduces Pathogenic Bacteria In Low-Order Streams, Sara A. Bolds, B. Graeme Lockaby, Latif Kalin, Stephen S. Ditchkoff, Mark D. Smith, Kurt Vercauteren
USDA Wildlife Services: Staff Publications
Invasive wild pig populations have undergone enormous increases in the United States and particularly across the southern U.S. in recent years. High fecundity rates and abilities to adapt quickly to varied habitats have enabled pig populations to become entrenched and difficult to eliminate. The pigs cause many negative impacts on ecosystems including degradation of water quality through infusion of fecal contamination and other non-point source pollutants. Our goal was to determine the effects of pig removal on water quality in streams that were known to be significantly polluted by pig activity Bolds (J Environ Qual 50: 441–453, 2021). We compared …
A Model For The Prediction Of Antimicrobial Resistance In Escherichia Coli Based On A Comparative Evaluation Of Fatty Acid Profiles, Randal S. Stahl, Bledar Bisha, Sebabrata Mahapatra, Jeffrey C. Chandler
A Model For The Prediction Of Antimicrobial Resistance In Escherichia Coli Based On A Comparative Evaluation Of Fatty Acid Profiles, Randal S. Stahl, Bledar Bisha, Sebabrata Mahapatra, Jeffrey C. Chandler
USDA Wildlife Services: Staff Publications
Antimicrobial resistance is a threat to agricultural production and public health. In this proof-of-concept study, we investigated predicting antimicrobial sensitive/resistant (S/R) phenotypes and host sources of Escherichia coli (n = 128) based on differential fatty acid abundance. Myristic (14:0), pentadecanoic acid (15:0), palmitic (16:0), elaidic (18:19) and steric acid (18:0) were significantly different (α = 0.05) using a two-way ANOVA for predicting nalidixic acid, ciprofloxacin, aztreonam, cefatoxime, and ceftazidime S/R phenotypes. Additionally, analyses of palmitoleic (16:1), palmitic acid (16:0), methyl palmitate (i-17:0), and cis-9,10-methyleneoctadecanoic acid (19:0Δ) showed these markers were significantly different (α = 0.05) between isolates obtained from cattle …
Monoclonal Antibodies To Avian Escherichia Coli Iss, Steven L. Foley, Shelley M. Horne, Catherine W. Giddings, Thomas R. Gustad, Erick D. Handegard, Michael Robinson, Lisa K. Nolan
Monoclonal Antibodies To Avian Escherichia Coli Iss, Steven L. Foley, Shelley M. Horne, Catherine W. Giddings, Thomas R. Gustad, Erick D. Handegard, Michael Robinson, Lisa K. Nolan
Lisa K. Nolan
Escherichia coli infections are a major problem for the poultry industry in the United States. Yet, the virulence mechanisms operative in avian E. coli are poorly understood. In the present studies, monoclonal antibodies (MAbs) have been generated that may facilitate study of the pathogenesis of avian colibacillosis. These MAbs are directed against the Iss protein because results from our laboratory have shown that the possession of iss DNA sequences is strongly correlated with the E. coli implicated in avian colibacillosis. As part of an overall effort to explore the role of iss/Iss in colibacillosis pathogenesis, Iss protein has been purified, …
Complement Resistance, As Determined By Viable Count And Flow Cytometric Methods, And Its Association With The Presence Of Iss And The Virulence Of Avian Escherichia Coli, Lisa K. Nolan, Catherine W. Giddings, Shelley M. Horne, Curt Doetkott, Penelope S. Gibbs, Richard E. Wooley, Steven L. Foley
Complement Resistance, As Determined By Viable Count And Flow Cytometric Methods, And Its Association With The Presence Of Iss And The Virulence Of Avian Escherichia Coli, Lisa K. Nolan, Catherine W. Giddings, Shelley M. Horne, Curt Doetkott, Penelope S. Gibbs, Richard E. Wooley, Steven L. Foley
Lisa K. Nolan
Previous work in our labs has shown that avian Escherichia coli virulence is correlated with resistance to complement. Also, our studies have revealed that the presence of the increased serum survival gene (iss), known to contribute to the complement resistance and virulence of mammalian E. coli, may predict the virulent nature of an avian E. coli isolate. This relationship warrants further research, but further clarification of the relationship among virulence, complement resistance, and iss sequences requires use of complement susceptibility assays. Such assays, unfortunately, are labor-intensive, expensive, and difficult to perform. In the present study, the results of two complement …
Location Of Increased Serum Survival Gene And Selected Virulence Traits On A Conjugative R Plasmid In An Avian Escherichia Coli Isolate, Timothy J. Johnson, Catherine W. Giddings, Shelley M. Horne, Penelope S. Gibbs, Richard E. Wooley, Jerod Skyberg, Pam Olah, Ronda Kercher, Julie S. Sherwood, Steven L. Foley, Lisa K. Nolan
Location Of Increased Serum Survival Gene And Selected Virulence Traits On A Conjugative R Plasmid In An Avian Escherichia Coli Isolate, Timothy J. Johnson, Catherine W. Giddings, Shelley M. Horne, Penelope S. Gibbs, Richard E. Wooley, Jerod Skyberg, Pam Olah, Ronda Kercher, Julie S. Sherwood, Steven L. Foley, Lisa K. Nolan
Lisa K. Nolan
Avian colibacillosis is a costly disease for the poultry industry. The mechanisms of virulence employed by the etiologic agent of this disease remain ill defined. However, accumulated evidence suggests that complement resistance and the presence of the increased serum survival gene (iss) in an avian Escherichia coli isolate may be indicative of its ability to cause disease. This association of iss with the E. coli implicated in avian disease may mean that iss and/or, perhaps, the genes associated with it are important contributors to avian E. coli virulence. For this reason, we have begun a search for iss's location in …
Virulence Factors Of Escherichia Coli From Cellulitis Or Colisepticemia Lesions In Chickens, J. S. Jeffrey, Lisa K. Nolan, K. H. Tonooka, S. Wolfe, C. W. Giddings, S. M. Horne, S. L. Foley, A. M. Lynne, J. O. Ebert, L. M. Elijah, G. Bjorklund, S. J. Pfaff-Mcdonough, R. S. Singer, C. Doetkott
Virulence Factors Of Escherichia Coli From Cellulitis Or Colisepticemia Lesions In Chickens, J. S. Jeffrey, Lisa K. Nolan, K. H. Tonooka, S. Wolfe, C. W. Giddings, S. M. Horne, S. L. Foley, A. M. Lynne, J. O. Ebert, L. M. Elijah, G. Bjorklund, S. J. Pfaff-Mcdonough, R. S. Singer, C. Doetkott
Lisa K. Nolan
This study was designed to compare virulence factors of cellulitis-derived Escherichia coli to colisepticemic E. coli in order to clarify whether E. coli associated with cellulitis comprise a unique subset of pathogenic E. coli. Isolates were tested for serotype, capsule, aerobactin production, colicin production, the presence of the iss gene, and serum resistance. Untypable isolates made up the greatest percentage of each group. Serotypes O2 and O78 were the most commonly identified among both groups of isolates. No statistical differences in the distribution of aerobactin or colicin production, capsule, or iss gene were observed between groups. Cluster analysis showed that …