Veterinary Physiology Commons^™

Effects Of 17Β Estradiol In The Metabolism And Morphology Of Bluegill Sunfish (Lepomis Macrochirus), Neeta Parajulee Karki

Masters Theses

Fish natural habitats are increasingly contaminated with various estrogenic compounds, including 17β estradiol (E2). This compound causes adverse effects on the reproductive system of male fish; however, the effects of E2 on other aspects of fish metabolism, morphology and histopathological changes in internal organs are not well known. The objective of this study is to evaluate the effects of E2 exposure on the basal and stressed metabolic rate, morphological changes in body shapes, and histological changes in the liver tissues of sunfish species. Fish were held individually in ten gallon tanks under two treatments of 40 and 80 ng/L and …

Generation And Use Of Monoclonal Antibodies To Ldentify Subpopulations Of Post Thaw Stallion Sperm, Sarah Abdulaziz Almuhanna

Masters Theses

Artificial insemination (AI) is commonly used for breeding of horses. Selecting high quality semen is critical to AI success but is difficult to achieve. In this study, we investigate the potential of generating antibodies against stallion sperm to identify subpopulations of post-thaw sperm based on surface markers. Female Balb/C mice are immunized with semen from a 24-year-old fertile stallion followed by booster immunizations each week for at least six weeks. Monoclonal antibodies are generated by fusion of myeloma cells to mouse spleen cells. Eight monoclonal antibodies resulting from these fusions are subdivided into four immunostaining patterns. These patterns differentially recognize …

Effects Of Methylxanthines On Newborn Rat Respiration, Craig Gatto

Masters Theses

Methylxanthines (MX), such as theophylline, are commonly used in the treatment of recurrent apnea due to their stimulant effects on the respiratory center. Structure-activity studies have demonstrated that substitution of alkyl groups on the 3-position of the xanthine nucleus results in increased bronchodilator potency, while substitution on the 1-position is important for adenosine antagonism and CNS stimulation. Three different alkylxanthines were studied to determine the structural requirements for respiratory stimulation. Respiratory rates and volumes were determined in 4- to 7-day-old rats using a volume displacement body plethysmograph. Measurements were made before and at 5 minute intervals after subcutaneous (s.c.) injection …

The Effects Of Xanthine And Adenosine Analogs On Rat Pup Diaphragmatic Contractility, Liam C. Farrell

Masters Theses

Methylxanthines are commonly used in the treatment of respiratory disorders such as asthma, apnea of prematurity and Cheyne-Stokes respiration. Recently, certain methylxanthines have been found to potentiate the contraction of the diaphragm and increase contraction strength of the fatigued diaphragm. The mechanism of action of the methylxanthines is unknown. Three theories have been proposed but each has its supportive and negative points. One theory is that methylxanthines inhibit cyclic AMP phosphodiesterase (PDE) which facilitates contraction by increasing cyclic AMP levels in the cell. Another theory is that methylxanthines antagonize adenosine, thus blocking the adenosine-induced inhibition of contraction. The third theory …

The Effects Of Insulin On Hepatic Glucocorticoid Receptor Content In The Diabetic Rat, Jeffrey John Yourick

Masters Theses

The streptozotocin-induced diabetic rat liver was analyzed for glucocorticoid receptor (GCR) content by a saturation and Scatchard analysis. A preliminary study was performed on the stabilization of the cytoplasmic GCR in a frozen cytosol preparation. The addition of 10% glycerol (V/V), 10 mM sodium molybdate, and 0.2 mg/ml trypsin inhibitor resulted in an approximate 20% sparing of GCR content upon freezing.

The hepatic GCR content of diabetic rats was significantly decreased from a control level of 0.1667 ± 0.0142 pmol/mg protein to 0.1094 ± 0.007 pmol/mg protein. Insulin replacement therapy to the diabetic rat dramatically increased the hepatic GCR content. …

The Effects Of Prostaglandin E2 On The Healing Processes Of Rat Perforated Tympanic Membranes, Diane Sue Saylor

Masters Theses

Prostaglandin E₂ (PGE₂) was topically administered to perforated rat tympanic membranes in an attempt to facilitate regrowth of the membranes. Every 24 hours for the first four postperforation days, two groups of tympanic membranes were processed and examined histologically for differences between PGE₂ treated and control animals.

At 24 hours postperforation granulocyte infiltration at the perforation site was greater in the test animals. By 48 hours blood clots were larger in PGE₂ treated animals. Finally, the epithelium was thinner in test animals than in control animals at 96 hours.

Slight tendencies for the test animals …

An In Vitro Study Of The Effects Of 17Β Estradiol On A Possible Atherosclerotic-Causing Enzyme, Xanthine Oxidase, Peter E. Malo

Masters Theses

No abstract provided.

The Effect Of Prostaglandins And Aspirin On Insulin Release From Isolated Normal Rat Islets, Barbara Ann Hopps

Masters Theses

No abstract provided.

Progesterone And Cortisol In The Hamster: Their Role In Parturition, As Well As The Growth And Lactogenesis Of The Mammary Glands, Anne Christine Flook

Masters Theses

No abstract provided.

Treflan And The Oxygen Consumption Of Green Sunfish (Lepomis Cyanellus), Barbara Jo Warner

Masters Theses

Oxygen consumption rates of 51 green sunfish (Lepomis cyanellus, Rafinesque) were monitored over five hours exposure to Treflan E.C. concentrations of 0, 0.32, 0.56, 1.0, and 2.0 ppm. There was a great variation in the responses of the fish at each concentration; and Student's t-tests revealed no significant differences. However, graphs of the mean hourly oxygen consumption rates showed certain trends. During the fifth hour of exposure, there appears to be an acclimation to the Treflan at concentrations of 0.32 and 0.56 ppm; but no such acclimation at the higher concentrations of 1.0 and 2.0 ppm. Hourly oxygen …

Metabolic Abnormalities In Liver And Pancreas Of A Diabetic (Toronto-Kk) Strain Of Mouse, Michael Clayton Appel

Masters Theses

No abstract provided.

The Effects Of Crowding On The Diurnal Ascorbic Acid Levels Of Albino Rats, Mary Jo Barr

Masters Theses

The adrenal physiology of the albino rat was studied through the measurement of adrenal ascorbic acid levels. A cyclic phenomenon was observed which follows the diel periodicity of the animal. Ascorbic acid values were found to be characteristically higher in males than in females. A similar difference was also seen in populations divided into young animals (body weight less than 175 grams) and old animals (body weight greater than 175 grams). Crowding, induced by manipulating the area of cage space per gram of body weight, was observed to be a definite stressor and caused the adrenal ascorbic acid levels to …

The Effects Of Acute Stress On Adrenal Ascorbic Acid Levels, Robert Joseph Collier

Masters Theses

The diurnal rhythm of adrenal ascorbic acid (DAR) displays an inverse relationship to the diurnal rhythm of circulating levels of plasma corticosteroids. Alloxan monohydrate, 175 mg/kg body weight was given subcutaneously to albino rats to determine the effect of the diabetic state on the DAR. A total of 200 female albino rats were divided into two study groups. Study Group I was composed of 150 animals divided into populations of 15, each including 3 controls. Study Group II was composed of populations of 5, each group containing all controls or all alloxinated rats. The populations of 15 were sacrificed at …

Immunological And Reproductive Responses In The Male Rat Following Challenge With Homologous Or Autologous Sperm, James M. Andress

Masters Theses

No abstract provided.

A Study Of Blood Constituent Levels And The Influence Of Certain Environmental Factors Upon Them In The Wild Mallard Duck, Christopher Louis Schatte

Masters Theses

No abstract provided.

The Effect Of Pregnancy And Lactation On Steroid-Induced Ulcers In The Rat, Paul A. Kelly

Masters Theses

No abstract provided.

Influence Of Some Stressors On Peripheral Blood Response To Estrogen Stimulation In The Female Mallard Duck, Morton S. Rickless

Masters Theses

No abstract provided.

Nickelous Nitrate Hexahydrate And Its Effect On Reproduction In The Mouse, Marvin R. Guthaus

Masters Theses

No abstract provided.