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Full-Text Articles in Veterinary Physiology
Effect Of Haemonchus Contortus Excretory/Secretory Protein On Differences In Host Neutrophil Migration, Hannah Grace Teddleton
Effect Of Haemonchus Contortus Excretory/Secretory Protein On Differences In Host Neutrophil Migration, Hannah Grace Teddleton
Graduate Theses, Dissertations, and Problem Reports (ETD)
An excretory/secretory (E/S) product, common to many helminths, is neutrophil inhibitory factor (NIF) which negatively affects neutrophil migration and activity. Due to differences in neutrophil accumulation between parasite resistant St. Croix STC and parasite susceptible Suffolk (SUF) sheep, we hypothesized that Hc-NIF may inhibit migration of SUF and not STC neutrophils. Previous studies have demonstrated that Hc produces NIF but is not commercially available. To test our hypothesis, neutrophils were cultured from STC and SUF sheep in the presence of Ancylostoma caninum-derived NIF (Ac-NIF) and measure chemotaxis to Interleukin-8 (IL-8), HcLA or H. contortus third-stage excretory/secretory (E/S) products. Neutrophils were …