Veterinary Physiology Commons^™

Generation And Use Of Monoclonal Antibodies To Ldentify Subpopulations Of Post Thaw Stallion Sperm, Sarah Abdulaziz Almuhanna

Masters Theses

Artificial insemination (AI) is commonly used for breeding of horses. Selecting high quality semen is critical to AI success but is difficult to achieve. In this study, we investigate the potential of generating antibodies against stallion sperm to identify subpopulations of post-thaw sperm based on surface markers. Female Balb/C mice are immunized with semen from a 24-year-old fertile stallion followed by booster immunizations each week for at least six weeks. Monoclonal antibodies are generated by fusion of myeloma cells to mouse spleen cells. Eight monoclonal antibodies resulting from these fusions are subdivided into four immunostaining patterns. These patterns differentially recognize …

The Effects Of Insulin On Hepatic Glucocorticoid Receptor Content In The Diabetic Rat, Jeffrey John Yourick

Masters Theses

The streptozotocin-induced diabetic rat liver was analyzed for glucocorticoid receptor (GCR) content by a saturation and Scatchard analysis. A preliminary study was performed on the stabilization of the cytoplasmic GCR in a frozen cytosol preparation. The addition of 10% glycerol (V/V), 10 mM sodium molybdate, and 0.2 mg/ml trypsin inhibitor resulted in an approximate 20% sparing of GCR content upon freezing.

The hepatic GCR content of diabetic rats was significantly decreased from a control level of 0.1667 ± 0.0142 pmol/mg protein to 0.1094 ± 0.007 pmol/mg protein. Insulin replacement therapy to the diabetic rat dramatically increased the hepatic GCR content. …

The Effects Of Prostaglandin E2 On The Healing Processes Of Rat Perforated Tympanic Membranes, Diane Sue Saylor

Masters Theses

Prostaglandin E₂ (PGE₂) was topically administered to perforated rat tympanic membranes in an attempt to facilitate regrowth of the membranes. Every 24 hours for the first four postperforation days, two groups of tympanic membranes were processed and examined histologically for differences between PGE₂ treated and control animals.

At 24 hours postperforation granulocyte infiltration at the perforation site was greater in the test animals. By 48 hours blood clots were larger in PGE₂ treated animals. Finally, the epithelium was thinner in test animals than in control animals at 96 hours.

Slight tendencies for the test animals …

An In Vitro Study Of The Effects Of 17Β Estradiol On A Possible Atherosclerotic-Causing Enzyme, Xanthine Oxidase, Peter E. Malo

Masters Theses

No abstract provided.

The Effect Of Prostaglandins And Aspirin On Insulin Release From Isolated Normal Rat Islets, Barbara Ann Hopps

Masters Theses

No abstract provided.

Progesterone And Cortisol In The Hamster: Their Role In Parturition, As Well As The Growth And Lactogenesis Of The Mammary Glands, Anne Christine Flook

Masters Theses

No abstract provided.

Metabolic Abnormalities In Liver And Pancreas Of A Diabetic (Toronto-Kk) Strain Of Mouse, Michael Clayton Appel

Masters Theses

No abstract provided.

Immunological And Reproductive Responses In The Male Rat Following Challenge With Homologous Or Autologous Sperm, James M. Andress

Masters Theses

No abstract provided.

A Study Of Blood Constituent Levels And The Influence Of Certain Environmental Factors Upon Them In The Wild Mallard Duck, Christopher Louis Schatte

Masters Theses

No abstract provided.

The Effect Of Pregnancy And Lactation On Steroid-Induced Ulcers In The Rat, Paul A. Kelly

Masters Theses

No abstract provided.

Influence Of Some Stressors On Peripheral Blood Response To Estrogen Stimulation In The Female Mallard Duck, Morton S. Rickless

Masters Theses

No abstract provided.

Nickelous Nitrate Hexahydrate And Its Effect On Reproduction In The Mouse, Marvin R. Guthaus

Masters Theses

No abstract provided.