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Articles 1 - 30 of 37

Full-Text Articles in Veterinary Microbiology and Immunobiology

Evaluation Of Chicken Serum And Chicken Egg Yolk As Novel Supplements For The In Vitro Cultivation Of Mycoplasma Hyorhinis, Yi-Chia Li, Sin-Hang Ma, Wei-Hao Lin, Chao-Nan Lin, Ming-Tang Chiou Dec 2025

Evaluation Of Chicken Serum And Chicken Egg Yolk As Novel Supplements For The In Vitro Cultivation Of Mycoplasma Hyorhinis, Yi-Chia Li, Sin-Hang Ma, Wei-Hao Lin, Chao-Nan Lin, Ming-Tang Chiou

The Thai Journal of Veterinary Medicine

Mycoplasma hyorhinis is a significant pathogen in swine, yet its fastidious nutritional requirements present challenges for in vitro cultivation. Conventional supplements like horse serum (HS) often suffer from batch variability and potential inhibitors. This study aimed to evaluate chicken serum (CS) and chicken egg yolk (CY) as novel alternatives to develop a reliable cultivation method for M. hyorhinis. Five clinical isolates were cultured for 10 days in a basal medium supplemented with CS or CY at 10%, 20%, or 30%, with 30% HS as the control. Growth was quantified daily using a Color Changing Unit (CCU) assay. CS supported …


Core And Characteristic Oral Microorganisms In The Giant Pandas (Ailuropoda Melanoleuca) Suggested Plenty Of Antibiotic Resistance Prevalence: A Mini Review, Han Long, Haifeng Liu, Caiwu Li, Xiaoping Ma, Guangneng Peng, Zhijun Zhong, Chengdong Wang, Ziyao Zhou Dec 2025

Core And Characteristic Oral Microorganisms In The Giant Pandas (Ailuropoda Melanoleuca) Suggested Plenty Of Antibiotic Resistance Prevalence: A Mini Review, Han Long, Haifeng Liu, Caiwu Li, Xiaoping Ma, Guangneng Peng, Zhijun Zhong, Chengdong Wang, Ziyao Zhou

The Thai Journal of Veterinary Medicine

The giant panda (Ailuropoda melanoleuca) is a world-recognized rare wildlife endemic to China. Oral microorganisms may cause dental and other systemic diseases. Additionally, antibiotic resistance in this cute mammal may spread widely to travelers. In this mini review, we illustrated the oral core microorganisms of the giant pandas and their characteristics, as well as their prevalence of antibiotic resistance genes. Although there is limited availability in panda sample collection, researchers have made great efforts to find the characteristics of the giant pandas’ oral microbiota, including Enterococcus faecium, Enterococcus faecalis, and Staphylococci, with antibiotic resistance genes optrA …


Biofilm Formation Of A Strain Of Bovine Escherichia Coli And Analysis Of Its Influencing Factors, Xin Zhang, Yunjiao Chen, Weijuan Jia, Zhilin Liu, Zelin Jia, Jiayu Cui, Huisheng Xiong, Yuhang Zhang, Xueli Wang Oct 2025

Biofilm Formation Of A Strain Of Bovine Escherichia Coli And Analysis Of Its Influencing Factors, Xin Zhang, Yunjiao Chen, Weijuan Jia, Zhilin Liu, Zelin Jia, Jiayu Cui, Huisheng Xiong, Yuhang Zhang, Xueli Wang

The Thai Journal of Veterinary Medicine

This study investigated the biofilm (BF) formation of a bovine-derived Escherichia coli (E. coli) strain (designated DL1907) and analyzed the factors influencing its formation. The BF-forming capacity, optimal formation time and temperature, as well as the effects of different culture media, pH levels, NaCl concentrations, and glucose concentrations, were assessed using the crystal violet method. The results demonstrated that the strain had a strong BF-forming capacity, achieving the most intact BF structure after 36 h of culture at 37°C. Trypticase Soy Broth (TSB) medium promoted the strongest BF formation compared to Luria-Bertani (LB) and Müller-Hinton Broth (MH) media. …


Molecular Identification Of Cryptosporidium Spp. Isolated From Dairy Calves In The Northern Region Of Algeria, Dadda Anes, Abdellah Mohamed Cherif, Aoun Leila, Chatanun Eamudomkarn, Khelef Djamel, Keeseon S Eom, Dongmin Lee, Barakaeli Abdieli Ndosi, Mohammed Mebarek Bia Oct 2025

Molecular Identification Of Cryptosporidium Spp. Isolated From Dairy Calves In The Northern Region Of Algeria, Dadda Anes, Abdellah Mohamed Cherif, Aoun Leila, Chatanun Eamudomkarn, Khelef Djamel, Keeseon S Eom, Dongmin Lee, Barakaeli Abdieli Ndosi, Mohammed Mebarek Bia

The Thai Journal of Veterinary Medicine

Cryptosporidium spp. is the most frequent agent in Algeria that causes neonatal diarrhea in calves, with high symptomatic diarrhea in pre-weaned dairy calves. The study aims to measure the prevalence, distribution, and genotype of Cryptosporidium-infected calves in one of Algeria's high milk production areas (North Central and Northeast). From 35 dairy cattle farms were selected 172 (77 with diarrheic and 95 without diarrheic symptoms) calves aged less than 60 days. Twenty-three farms (65.71%) were detected with cryptosporidiosis using a modified Ziehl-Neelsen stain, whereby 68 calves (39.53%) were obtained positive. The Copro-ELISA test gave an individual prevalence of (37.66%, 22.11%) …


Monitoring Of African Horse Sickness Virus-Specific Antibodies In Horses, Northeastern Thailand, Napawan Bunpapong, Kitikhun Udom, Ekkapat Chamsai, Patchara Phuektes, Suphattra Jittimanee, Suphannika Phutthachalee, Supassama Chaiyawong, Kamonpan Charoenkul, Alongkorn Amonsin Prof. Dr. Jul 2025

Monitoring Of African Horse Sickness Virus-Specific Antibodies In Horses, Northeastern Thailand, Napawan Bunpapong, Kitikhun Udom, Ekkapat Chamsai, Patchara Phuektes, Suphattra Jittimanee, Suphannika Phutthachalee, Supassama Chaiyawong, Kamonpan Charoenkul, Alongkorn Amonsin Prof. Dr.

The Thai Journal of Veterinary Medicine

African horse sickness (AHS) is caused by infection with the African horse sickness virus (AHSV). In March 2020, AHS was first reported in Thailand due to the importation of subclinically infected host animals, such as zebras. In this study, we conducted a serological survey for AHSV-specific antibodies across 21 horse farms in the northeastern provinces (n=3) of Thailand. A total of 600 serum samples were collected for cross-sectional (n = 292) and longitudinal serological monitoring (n = 308). A cross-sectional study was conducted in 2020, during which serum samples (n = 292) were collected and tested for AHS antibodies using …


Genetic Characterization Of Lpai-H4n2 And H4n8 Viruses Recovered From Free-Grazing Ducks In Thailand, Supanat Boonyapisitsopa, Supassama Chaiyawong, Kamonpan Charoenkul, Kitikhun Udom, Ekkapat Chamsai, Napawan Bunpapong, Somsak Pakpinyo, Alongkorn Amonsin Prof. Dr. Jun 2025

Genetic Characterization Of Lpai-H4n2 And H4n8 Viruses Recovered From Free-Grazing Ducks In Thailand, Supanat Boonyapisitsopa, Supassama Chaiyawong, Kamonpan Charoenkul, Kitikhun Udom, Ekkapat Chamsai, Napawan Bunpapong, Somsak Pakpinyo, Alongkorn Amonsin Prof. Dr.

The Thai Journal of Veterinary Medicine

nfluenza A viruses (IAVs) can infect birds and various mammals. In this study, we retrospectively isolated and characterized IAVs (n=8) from oropharyngeal and cloacal swab samples of free-grazing ducks collected during December 2018 to December 2021, by using egg inoculation and whole-genome sequencing. The IAVs could be subtyped as IAV-H4N2 (n = 3) and IAV-H4N8 (n = 5) using real-time RT-PCR assays. Genetic and phylogenetic analyses revealed that all eight genes of Thai IAV-H4 belong to the Avian Eurasian lineage. Both Thai IAV-H4N2 and IAV-H4N8 exhibited low pathogenic avian influenza (LPAI) characteristics and were susceptible to oseltamivir and amantadine. This …


Antibiotic Resistance Profile Of Enterobacteriaceae Isolated From Healthy Pigs In The Republic Of Armenia, Yura Shirvanyan, Irma Artushyan, Lilit Hovsepyan, Ashkhen Shirvanyan, Elmira Ghazaryan, Tigran Markosyan Mar 2025

Antibiotic Resistance Profile Of Enterobacteriaceae Isolated From Healthy Pigs In The Republic Of Armenia, Yura Shirvanyan, Irma Artushyan, Lilit Hovsepyan, Ashkhen Shirvanyan, Elmira Ghazaryan, Tigran Markosyan

The Thai Journal of Veterinary Medicine

The rising prevalence of antimicrobial resistance (AMR) among Enterobacteriaceae in livestock presents a significant threat to both animal and human health. This study investigates the antibiotic resistance profiles of Enterobacteriaceae isolated from 160 samples collected from the lymph nodes of healthy pigs in Armenia. Fifty isolates were identified, including Escherichia coli (24), Klebsiella spp. (10), Salmonella spp. (6), Raoultella ornithinolytica (4), Pseudomonas aeruginosa (4), and Proteus mirabilis (2). Resistance was evaluated using the disk diffusion method against 22 antibiotics from nine classes. Alarmingly, 94.5% of isolates were resistant to clarithromycin and 90.3% to erythromycin, while resistance to cephalothin and amoxicillin …


Prevalence Study Of Feline Ab Blood Types In Bangkok And Vicinities Of Thailand By Rapid Immunochromatographic Test During 2022-2024, Suwarath Wadeerat, Sinittha Thongwandi, Thitima Tripipat, Teerawut Nedumpun Mar 2025

Prevalence Study Of Feline Ab Blood Types In Bangkok And Vicinities Of Thailand By Rapid Immunochromatographic Test During 2022-2024, Suwarath Wadeerat, Sinittha Thongwandi, Thitima Tripipat, Teerawut Nedumpun

The Thai Journal of Veterinary Medicine

The AB blood group system is a key classification for feline blood types, categorizing them as type A, B, or AB based on red blood cell surface antigens. This study aimed to update the prevalence of feline blood types among domestic and pedigreed cats in Bangkok and vicinities from 2022 to 2024. A total of 114 blood samples, including 76 Domestic Shorthairs (DSH) and 38 pedigreed cats, including 10 breeds, were analyzed using the rapid immunochromatographic feline blood typing. Results revealed that blood type A was predominant, present in 93.86% of cats, consistent with global findings. Blood types B and …


Immuno-Bioinformatics Study Of Secondary Metabolites From Watercress Nasturtium Officinale As Immunostimulant In Whiteleg Shrimp Litopenaeus Vannamei By Targeting Peroxinectin And Scavenger Receptor Class B, Qurrota A’Yunin, Muhaimin Rifa’I, Maftuch Maftuch, Yoga Dwi Jatmiko, Muhammad Hermawan Widyananda, Triyanto Triyanto, Muhammad Tegar Handrianto Jan 2025

Immuno-Bioinformatics Study Of Secondary Metabolites From Watercress Nasturtium Officinale As Immunostimulant In Whiteleg Shrimp Litopenaeus Vannamei By Targeting Peroxinectin And Scavenger Receptor Class B, Qurrota A’Yunin, Muhaimin Rifa’I, Maftuch Maftuch, Yoga Dwi Jatmiko, Muhammad Hermawan Widyananda, Triyanto Triyanto, Muhammad Tegar Handrianto

Karbala International Journal of Modern Science

The viral disease affects the whiteleg shrimp (Litopenaeus vannamei) and causes losses. Immunostimulants are recognized as a more environmentally friendly approach to disease management and antimicrobial properties of aquatic organisms. Watercress (Nasturtium officinale) is an aquatic plant species that contains antioxidant properties that can protect the organism from disease and boost the immune system. Shrimp rely heavily on innate immunity to combat infectious agents due to their absence of adaptive immunity. Peroxinectin and scavenger receptor class B (SRB) play essential roles in the shrimp defense system. This research employs a bioinformatic to assess the immunostimulatory potential …


Correlation Of Classical Swine Fever (Csf) Antibody Protective Level Detected By Enzyme-Linked Immunosorbent Assay And Serum Neutralization Test, Chairani Ridha Maghfira, Nguyen Thanh Che, Apisit Kittawornrat, Roongroje Thanawongnuwech, Yaowalak Panyasing Jan 2025

Correlation Of Classical Swine Fever (Csf) Antibody Protective Level Detected By Enzyme-Linked Immunosorbent Assay And Serum Neutralization Test, Chairani Ridha Maghfira, Nguyen Thanh Che, Apisit Kittawornrat, Roongroje Thanawongnuwech, Yaowalak Panyasing

The Thai Journal of Veterinary Medicine

Protective antibody against classical swine fever virus (CSFV) is an important parameter used for disease monitoring in CSFV-endemic areas. Enzyme-linked immunosorbent assay (ELISA) is a simple and practical serological assay for CSFV antibody detection. However, the use of CSFV ELISAs in CSFV antibody-positive herds, i.e., CSFVvaccinated or -previously infected herds, was limited by an application of the test results. This study aimed to evaluate the correlation and estimation of protective antibody levels detected by ELISA and serum neutralization assay. A total of 522 negative and positive serums were tested by SN and ELISA in parallel. Comparisons of sample-to-positive (S/P) values …


Evaluation Of The Presence Of Sarcocystis Spp. In Dogs Raised In Alpaca Farm Enterprises In Cajamarca, Peru, Milena Ydrogo, María Cabrera, Jennifer L. Cuzcano-Anarcaya, Luis A. Vargas-Rocha, Teófilo Torrel Dec 2024

Evaluation Of The Presence Of Sarcocystis Spp. In Dogs Raised In Alpaca Farm Enterprises In Cajamarca, Peru, Milena Ydrogo, María Cabrera, Jennifer L. Cuzcano-Anarcaya, Luis A. Vargas-Rocha, Teófilo Torrel

The Thai Journal of Veterinary Medicine

The apicomplexans of the genus Sarcocystis are parasites that infect various animal species, including humans. Both dogs and other carnivores can act as definitive hosts of this protozoan. The present study aimed to determine the frequency of Sarcocystis spp. in domestic dogs raised in three alpaca breeding areas in Cajamarca. Random fecal samples were collected from 102 dogs of different ages and both sexes in three locations related to alpaca breeding: Atahualpa Jerusalén Agricultural Workers Cooperative - Cajamarca (N = 35), SAIS Huacraruco - San Juan (N = 33), and Alpacas Project - Foncreagro - Sorochuco (N = 34). The …


The Relationship Between Periodontal Disease Of Feline And Oral Core Microbiome, Ziyao Zhou Dec 2024

The Relationship Between Periodontal Disease Of Feline And Oral Core Microbiome, Ziyao Zhou

The Thai Journal of Veterinary Medicine

The microbiome of the oral cavity is complex with the average adult harboring about 50 to 100 billion bacteria which represent about 200 predominant bacterial species. There are a large number of microbes in the oral cavity of the feline, normally beneficial to oral health and safety maintenance. However, when the balance of bacteria lost, it may occur feline stomatitis and periodontitis, which seriously endanger local oral health. Environmental implication, feeding model, age and genetic factors may be main factors affecting oral composition of microbiome. Filifactor alocis, Porphyromonas gingivals, Tannerella forsythia and their interactions are main pathogen that …


Multidrug-Resistance In Escherichia Coli And Klebsiella Pneumoniae Isolated From Broiler Chickens In Eastern Thailand, Suttitas Tongkamsai, Wachirawit Chatmontee, Kulchai Nakbubpa Dec 2024

Multidrug-Resistance In Escherichia Coli And Klebsiella Pneumoniae Isolated From Broiler Chickens In Eastern Thailand, Suttitas Tongkamsai, Wachirawit Chatmontee, Kulchai Nakbubpa

The Thai Journal of Veterinary Medicine

Broiler chickens have been suggested as an important source of antibiotic resistance Escherichia coli (E. coli) and Klebsiella pneumoniae (K. pneumoniae). The coinfection of multiple species of bacteria provides a specific ecological niche for the plasmid-mediated exchange of antibiotic-resistance genes. However, there is limited information about antimicrobial resistance (AMR) phenotypes of E. coli and K. pneumoniae isolates from broiler chickens. This study aimed to isolate E. coli and K. pneumoniae from broiler chicken farms in Thailand by observing the antibiotic resistance profiles and detecting the coexistence of these two bacteria. A total of 26 E. coli and 29 …


Bovine Respiratory Syncytial Virus: First Identification In Eastern Algeria Based On Direct Immunofluorescence And Elisa Tests, Imene Abdelmalek, Bakir Mamache, Amine Boudaoud, Yacine Khanfri Sep 2024

Bovine Respiratory Syncytial Virus: First Identification In Eastern Algeria Based On Direct Immunofluorescence And Elisa Tests, Imene Abdelmalek, Bakir Mamache, Amine Boudaoud, Yacine Khanfri

The Thai Journal of Veterinary Medicine

Bovine respiratory syncytial virus (BRSV) is the main agent of the bovine respiratory disease complex (BRD). Its circulation among the Algerian bovine population has been strongly suspected but never confirmed. This study investigated the prevalence of BRSV in cattle in eastern Algeria. From 2022 to 2023, a total of 225 serum and 102 lung tissue samples were collected from farms and slaughtered cattle of different ages. Samples were tested for BRSV antibodies by Enzyme-Linked Immunosorbent Assay (ELISA) and BRSV antigens by Direct Immunofluorescence Test (DFAT). Histopathological examination was also performed on lung tissue sections. BRSV-specific antibodies were detected in 91.11% …


Bioinformatics Analysis And Comparison Of The S Protein Of Canine Coronavirus And Feline Coronavirus, Bo Dong, Xiaowei Zhong, Jiaqi Zhuo, Renyi Hu, Xiaodong Zhang, Xiaolian Xu, Haiyan Deng, Xiaofang Yan, Weiming Lin Sep 2024

Bioinformatics Analysis And Comparison Of The S Protein Of Canine Coronavirus And Feline Coronavirus, Bo Dong, Xiaowei Zhong, Jiaqi Zhuo, Renyi Hu, Xiaodong Zhang, Xiaolian Xu, Haiyan Deng, Xiaofang Yan, Weiming Lin

The Thai Journal of Veterinary Medicine

The Coronavirus S protein induces neutralizing antibodies, specific receptor binding, and membrane fusion between the virus and host cells. These changes are crucial to understanding the mechanism, genetic correlates, and prophylactic treatment of viral infection. In this study, the spike (S) protein structure and phylogenetic association of CCoV and FcoV were analyzed using bioinformatics. The results showed that the S proteins of CCoV and FCoV contained transmembrane regions and signal peptides. The S proteins of these coronaviruses were soluble. The S proteins of these strains also contained two functional domains and two motifs, i.e., corona_S1 and corona_S2. Phylogenetic analysis showed …


Multidrug-Resistant Esbl-Producing Escherichia Coli Coexisting With Colistin-Resistance Genes In Pig Farms, Central Thailand, Wipawee Songsaeng, Nutthee Am-In, Nuvee Prapasarakul, Wandee Sirichokchatchawan Aug 2024

Multidrug-Resistant Esbl-Producing Escherichia Coli Coexisting With Colistin-Resistance Genes In Pig Farms, Central Thailand, Wipawee Songsaeng, Nutthee Am-In, Nuvee Prapasarakul, Wandee Sirichokchatchawan

The Thai Journal of Veterinary Medicine

The presence of extended-spectrum beta-lactamase and colistin resistance E. coli in food-producing animals is a public health concern. This study was conducted using a rigorous methodology to survey the prevalence of ESBL-producing and colistin-resistant E. coli from pig farms in the central part of Thailand. A total of 519 samples were collected from fecal, feed, and waste in 53 farms located in central Thailand. All samples were inoculated onto selective agar, and species identification was performed using biochemical assays and MAlDI-TOF MS. Antimicrobial susceptibility was determined by broth microdilution, and ESBL production was confirmed by the combination disc diffusion method. …


Preventive Effects Of Quercetin On The Leaky Gut By Suppression Of Il-1beta-Induced Transcription Factors Involved In An Inflammatory Pathway In Colonic Epithelial Cells, Wannaporn Chayalak, Sumpun Thammacharoen, Chatsri Deachapunya, Sutthasinee Poonyachoti Aug 2024

Preventive Effects Of Quercetin On The Leaky Gut By Suppression Of Il-1beta-Induced Transcription Factors Involved In An Inflammatory Pathway In Colonic Epithelial Cells, Wannaporn Chayalak, Sumpun Thammacharoen, Chatsri Deachapunya, Sutthasinee Poonyachoti

The Thai Journal of Veterinary Medicine

Flavonol quercetin (Q), found in vegetables and fruits, demonstrates the most potent anti-inflammatory effect. The preventive effect and cellular mechanisms of Q (Q1, Q10, and Q100 µM) on IL-1β induced leaky gut via the signaling molecules of inflammatory pathway, NF-κB p65-iκB/or p38/ERK1/2/MLCK/phospho(p)-MLC were investigated in Caco-2 cells. The cytotoxic effect of Q was initially determined by MTT assay. Transepithelial electrical resistance (TER) and apical-to-basolateral fluorescein isothiocyanate-labeled dextran (FD-4) flux were used to determine paracellular permeability. The protein expression of signaling molecules was examined by Western blot analysis, and their target MLCK mRNA expression was determined using real-time PCR. All Q …


A Case Of Conjunctivitis Due To Mdr-Mr Staphylococcus Pseudintermedius In A Dog, Hazel Tamakan Yesi̇lovali, Hali̇t Sukur Aug 2024

A Case Of Conjunctivitis Due To Mdr-Mr Staphylococcus Pseudintermedius In A Dog, Hazel Tamakan Yesi̇lovali, Hali̇t Sukur

The Thai Journal of Veterinary Medicine

A young male Dachshund dog who came to the animal hospital was diagnosed with conjunctivitis on clinical examination. Methicillin-resistant Staphylococcus pseudintermedius was identified as a result of culture and bacteriological examination of the conjunctival swab, and then antimicrobial susceptibility test. Methicillin resistant S. pseudintermedius identified with the VITEK-II Compact device was also confirmed by molecular methods so that susceptible antibiotics were selected for the treatment of the patient and reported. In accordance with the antimicrobial susceptibility results, the patient was treated with an eye drop preparation containing neomycin. This is the first report of a canine conjunctivitis caused by multidrug-methicillin …


Characteristics Of Extended-Spectrum Beta-Lactamase (Esbl)- Producing Escherichia Coli Associated With Antimicrobial Use In Captive Wild Animals, Montira Yossapol, Jinnapat Imidom, Kannarun Sakhorn, Pimpatcha Thanomwat, Sarawut Sonrit, Siraphop Labmala, Tarid Purisotayo, Natapol Pumipuntu, Justice Opare Odoi, Adithep Konputtar Aug 2024

Characteristics Of Extended-Spectrum Beta-Lactamase (Esbl)- Producing Escherichia Coli Associated With Antimicrobial Use In Captive Wild Animals, Montira Yossapol, Jinnapat Imidom, Kannarun Sakhorn, Pimpatcha Thanomwat, Sarawut Sonrit, Siraphop Labmala, Tarid Purisotayo, Natapol Pumipuntu, Justice Opare Odoi, Adithep Konputtar

The Thai Journal of Veterinary Medicine

Antimicrobial agents are a cause of the increase in antimicrobial-resistant (AMR) bacteria. We investigated the prevalence and characteristics of extended-spectrum beta-lactamase (ESBL)-producing bacteria in captive wild animals before and after exposure to antimicrobial agents. Sixty-six fecal samples were collected from captive animals (spotted deer, Rusa deer, geese, chickens, rabbits, and guinea pigs) at the Mahasarakham University Mini Zoo in Thailand on three sampling occasions: October 2021, January 2022, and April 2022. Using MacConkey agar supplemented with cefotaxime, ESBL-producing Escherichia coli (ESBL-Ec) was isolated and subjected to antimicrobial susceptibility tests and genetic analysis. The results showed a high prevalence of ESBL-Ec …


A Potential Of Watercress Nasturtium Officinale Bioactive Compounds In Inhibiting Infectious Myonecrosis Virus (Imnv) By Targeting Rna-Dependent Rna Polymerase (Rdrp) Virus From Several Countries: In Silico Approach, Qurrota A’Yunin, Fatchiyah Fatchiyah, Maftuch Maftuch, Feri Eko Hermanto, Muhammad Hermawan Widyananda, Narendra Santika Hartana, Muhaimin Rifa’I, Yoga Dwi Jatmiko May 2024

A Potential Of Watercress Nasturtium Officinale Bioactive Compounds In Inhibiting Infectious Myonecrosis Virus (Imnv) By Targeting Rna-Dependent Rna Polymerase (Rdrp) Virus From Several Countries: In Silico Approach, Qurrota A’Yunin, Fatchiyah Fatchiyah, Maftuch Maftuch, Feri Eko Hermanto, Muhammad Hermawan Widyananda, Narendra Santika Hartana, Muhaimin Rifa’I, Yoga Dwi Jatmiko

Karbala International Journal of Modern Science

Infectious myonecrosis virus (IMNV) disease causes mass mortality and decreased shrimp production. The RdRp region projects to the interior, where it may function in transcription. The focus of this study was to determine the effect of amino acid polymorphisms from several countries on the structure of RdRp and identify the potential of watercress in inhibiting IMNV by targeting the RdRp protein of IMNV through an in silico approach. The results showed that the structure of the IMNV RdRp protein from Indonesia was similar to Mexico, and the protein structure from India_QDN was identical to India_QIL. Ligand binding affinity values showed …


Chicken Immune Responses To Vaccination By The Avian Influenza Subtype H5n1 Against Avian Influenza, Ramlah Ramlah, Sri Murtini, Retno Damayanti Soejoedono, Ketut Karuni Nyanakumari Natih, Siti Gusti Ningrum Sep 2023

Chicken Immune Responses To Vaccination By The Avian Influenza Subtype H5n1 Against Avian Influenza, Ramlah Ramlah, Sri Murtini, Retno Damayanti Soejoedono, Ketut Karuni Nyanakumari Natih, Siti Gusti Ningrum

Makara Journal of Science

Avian Influenza (AI) is one of the strategic animal diseases still considered a priority for control by the Indonesian government. This study aimed to examine the immune response of chickens to various types of H5N1 subtype AI vaccines in Indonesia and to determine the correlation of factors influencing the post-vaccination AI antibody response. Serum samples were tested using the hemagglutination inhibition (HI) test with the standard AI antigen subtype H5N1 strain A/Chicken/Barru/BBVM/41-13/2013 (Clade 2.1.3) and strain A/Chicken/Semarang/04141225-07/2014 (Clade 2.3.2). Antibody titer was calculated using the Geometric Mean Titer (GMT). The correlation analyses were performed to assess the correlation of antibody …


Assessment Of Trichostrongyles Resistance Against Anthelmintics Commonly Used In Sheep Reared In Dakahlia Governorate, Egypt, Mahmoud R. Saleh, Ibrahim Abbas, Moustafa Al-Araby, Salah Abu-Elwafa Jan 2023

Assessment Of Trichostrongyles Resistance Against Anthelmintics Commonly Used In Sheep Reared In Dakahlia Governorate, Egypt, Mahmoud R. Saleh, Ibrahim Abbas, Moustafa Al-Araby, Salah Abu-Elwafa

Mansoura Veterinary Medical Journal

Objective: To investigate, for the first time, the anthelmintic resistance of different trichostrongle-infecting sheep in the Dakahlia governorate, Egypt.

Design: Randomized controlled study.

Animals: 820 sheep were reared within 10 free-grazing herds with infrequent deworming practices.

Procedures: Fresh fecal samples were collected, and their floats were examined for trichostrongyle eggs, which were then identified using fecal cultures and/or ITS2-PCR amplification. Sheep (n=500) whose samples had egg counts ≥ 150 eggs per gram (EPG) of feces were selected and divided in each herd into five equal groups; each group received one of the tested anthelmintics, including albendazole (5 mg/kg BW), levamisole …


Time Course Investigation Of The Dermal Leukocyte Response To Lipoteichoic Acid In Chickens, Ian M. Gilbert, Jossie M. Santamaria, Gisela F. Erf Jan 2022

Time Course Investigation Of The Dermal Leukocyte Response To Lipoteichoic Acid In Chickens, Ian M. Gilbert, Jossie M. Santamaria, Gisela F. Erf

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Lipoteichoic acid (LTA) is a cell-wall polymer in Gram-positive bacteria that stimulates inflammation. Few studies have investigated in vivo immune response to LTA, and none of the in vivo studies have been performed in birds. For this project, the pulp (a skin derivative) of growing feathers (GF) of chickens was used to investigate the in vivo effects of intradermally injected LTA. In Study 1, GF of chickens were injected with 10 μL of differing concentrations of LTA (0.1, 1.0, 10, 100 or 250 μg LTA/mL; 3 chickens/dose). Growing feathers were plucked before injection (0 h) and at 6, 24, 48, …


New Approach To Health And The Environment To Avoid Future Pandemics, Serge Morand Jan 2020

New Approach To Health And The Environment To Avoid Future Pandemics, Serge Morand

Animal Sentience

This commentary expands Wiebers & Feigin’s target article by pinpointing how declining wildlife, expanding livestock and globalisation contribute to the increase in epidemics of zoonotic diseases, the COVID-19 crisis and future health crises. Epidemics and the emergence of zoonoses are manifestations of dysfunctional links with animals, both wild and domestic, requiring a new approach to health and the environment.


Rehabilitation Of An Injured Golden Eagle (Aquila Chrysaetos): A Case Study, Fida Muhammad Khan, Fakhar-I- Abbas, Abu Ul Hassan Faiz Sep 2017

Rehabilitation Of An Injured Golden Eagle (Aquila Chrysaetos): A Case Study, Fida Muhammad Khan, Fakhar-I- Abbas, Abu Ul Hassan Faiz

Journal of Bioresource Management

Habitat destruction and anthropogenic activities cause wild birds to migrate towards urban areas in search of food and sometimes nesting in high-roof buildings, where they are caught by local people using different techniques and are further used for hunting or recreational purposes. An injured Golden Eagle (Aquila chrysaetos) was found entangled in bushes near the vicinity of Balkasar Research Complex, Chakwal, Pakistan. After complete physical examination, a wound on the right wing and closed leg fracture was diagnosed. The successful treatment was done by anti-parasitic and anti-bacterial drugs with careful monitoring. Splint was adjusted after aligning the fractured …


'Dermo' Fleece Rot And Body Strike On Sheep, S G. Gherardi, S. S. Sutherland, N. Monzu Jan 1985

'Dermo' Fleece Rot And Body Strike On Sheep, S G. Gherardi, S. S. Sutherland, N. Monzu

Journal of the Department of Agriculture, Western Australia, Series 4

Blowfly strike is one of the major problems confronting the sheep industry in Austrsalia, with an estimated totsalcost of control eceeding $100 million each year.

Sheep are susceptible to five types of strike: body, breach, poll, pizzle and wound. Body strike, is of greater economic importance because its sporadic occurence from year to year makes it difficult to predict and cintrol. Outbreaks of body strike after rain can affect large numbers of sheep and inflict severe production losses.

In eastern Australia fleece rot is generally considered to be the main predisposing factor to blowfly strike, but in Western Australia evidence …


New Developments In Footrot Control, R B. Richards, L. J. Depiazzi, R. V. R. Gwynn Jan 1984

New Developments In Footrot Control, R B. Richards, L. J. Depiazzi, R. V. R. Gwynn

Journal of the Department of Agriculture, Western Australia, Series 4

The eradication policy for footrot in sheep adopted by the Western Australian Department of Agriculture has produced an overall decline in the number of properties with the disease.

Our knowledge of the disease has improved substantially as a result of the research conducted at the Slabany Regional Veterinary Laboratory in recent years. Laboratory tests now sssist stock inspectors and veterinary officers to distinguish between different forms of footrot and to quarantine and eradicate accordingly.

The recent discovery of more effective foot-bathing solutions by officers of the Victorian Department of Agriculture will greatly assist further eradication procedures.

Western Australia's sheep industry …


Mycotic Dermatitis (Lumpy Wool) Of Sheep, M R. Gardiner Jan 1971

Mycotic Dermatitis (Lumpy Wool) Of Sheep, M R. Gardiner

Journal of the Department of Agriculture, Western Australia, Series 4

MYCOTIC dermatitis is a common disease of the skin and overlying wool of sheep (and other herbivorous animals).

The photographs show the typical crusts and scabs which result from infection of skin, hair and wool follicles by the mycotic dematitis organism.

This article reviews the causes of lumpy wool infection and sets out control measures.


Infectious Sinusitis Of Turkeys, J Edgar Jan 1971

Infectious Sinusitis Of Turkeys, J Edgar

Journal of the Department of Agriculture, Western Australia, Series 4

Climatic conditions in W.A. are ideal for turkey production if advantage is taken of the wide open spaces. Over-crowded, poorly ventilated quarters are ideal for the spread of infectious sinusitis, and the disease, once established, is hard to eliminate without a complete change of birds.

This article provides advice for the prevention of this important disease at a time when turkey raising is increasing in W.A.


Bluetongue Of Sheep, M R. Gardiner Jan 1968

Bluetongue Of Sheep, M R. Gardiner

Journal of the Department of Agriculture, Western Australia, Series 4

BLUETONGUE is a serious disease of sheep, mainly of introduced European breeds, throughout the African continent.

It is maintained there by wild and domestic ruminants in the presence of certain insect vectors (carriers).

The disease has probably existed there from immemorial times among the indigenous ruminants, emerging as a recognised disease only when the much more susceptible European breeds were brought into the continent.