Veterinary Microbiology and Immunobiology Commons^™

Evaluating Potential Effects Of Solar Power Facilities On Wildlife From An Animal Behavior Perspective, Rachel Y. Chock, Barbara Clucas, Elizabeth K. Peterson, Bradley Blackwell, Daniel T. Blumstein, Kathleen Church, Esteban Fernández-Juricic, Gabriel Francescoli, Alison L. Greggor, Paul Kemp, Gabriela M. Pinho, Peter M. Sanzenbacher, Bruce A. Schulte, Pauline Toni

United States Department of Agriculture Wildlife Services: Staff Publications

Solar power is a renewable energy source with great potential to help meet increasing global energy demands and reduce our reliance on fossil fuels. However, research is scarce on how solar facilities affect wildlife. With input from professionals in ecology, conservation, and energy, we conducted a research-prioritization process and identified key questions needed to better understand impacts of solar facilities on wildlife. We focused on animal behavior, which can be used to identify population responses before mortality or other fitness consequences are documented. Behavioral studies can also offer approaches to understand the mechanisms leading to negative interactions (e.g., collision, singeing, …

Environmental Correlates Of Genetic Variation In The Invasive European Starling In North America, Natalie R. Hofmeister, Scott J. Werner, Irby J. Lovette

United States Department of Agriculture Wildlife Services: Staff Publications

Populations of invasive species that colonize and spread in novel environments may differentiate both through demographic processes and local selection. European starlings (Sturnus vulgaris) were introduced to New York in 1890 and subsequently spread throughout North America, becoming one of the most widespread and numerous bird species on the continent. Genome-wide comparisons across starling individuals and populations can identify demographic and/or selective factors that facilitated this rapid and successful expansion. We investigated patterns of genomic diversity and differentiation using reduced-representation genome sequencing of 17 winter-season sampling sites. Consistent with this species' high dispersal rate and rapid expansion history, …

Temporal And Spatial Blood Feeding Patterns Of Urban Mosquitoes In The San Juan Metropolitan Area, Puerto Rico, Matthew W. Hopken, Limarie J. Reyes-Torres, Nicole Scavo, Antoinette J. Piaggio, Zaid Abdo, Daniel Taylor, James Pierce, Donald A. Yee

United States Department of Agriculture Wildlife Services: Staff Publications

Simple Summary: Understanding the biodiversity of urban ecosystems is critical for management of invasive and pest species, conserving native species, and disease control. Mosquitoes (Culicidae) are ubiquitous and abundant in urban ecosystems, and rely on blood meals taken from vertebrates. We used DNA from freshly blood-fed mosquitoes to characterize the diversity of vertebrate host species in the San Juan Metropolitan Area, Puerto Rico. We collected two mosquito species that fed on a variety of vertebrates. Culex quinquefasciatus fed on 17 avian taxa (81.2% of blood meals), seven mammalian taxa (17.9%), and one reptilian taxon (0.85%). Aedes aegypti blood meals were …

Population Genomic Transformations Induced By Isolation Of Wild Bird Avian Influenza Viruses (Orthomyxoviridae) In Embryonated Chicken Eggs, Matthew W. Hopken, Antoinette J. Piaggio, K. L. Pabilonia, James Pierce, Theodore Anderson, Courtney Pierce, Zaid Abdo

United States Department of Agriculture Wildlife Services: Staff Publications

Isolation and cultivation of wild-type viruses in model organism cells or tissues is standard practice in virology. Oftentimes, the virus host species is distantly related to the species from which the culture system was developed. Thus, virus culture in these tissues and cells basically constitutes a host jump, which can lead to genomic changes through genetic drift and/or adaptation to the culture system. We directly sequenced 70 avian influenza virus (Orthomyxoviridae) genomes from oropharyngeal/cloacal swabs collected from wild bird species and paired virus isolates propagated from the same samples following isolation in specific-pathogen-free embryonated chicken eggs. The data were analyzed …

Inefficiency Of Anthraquinone-Based Avian Repellents When Applied To Sunflower: The Importance Of Crop Vegetative And Floral Characteristics In Field Applications, Brandon Kaiser, Burton L. Johnson, Mike Ostlie, Scott J. Werner, Page E. Klug

United States Department of Agriculture Wildlife Services: Staff Publications

BACKGROUND: Blackbirds (Icteridae) cause significant damage to sunflower (Helianthus annuus L.) prompting the need for effective management tools. Anthraquinone-based repellents can reduce feeding by > 80% in laboratory settings, but require birds to learn the negative association through repellent ingestion. We evaluated an anthraquinone-based repellent applied directly to mature sunflower plants for its ability to reduce bird damage. We used captive male red-winged blackbirds (Agelaius phoeniceus) to evaluate efficacy of two anthraquinone-based formulations in varying concentrations and applied in a manner attainable by sunflower producers. We also assessed field application methods for repellent coverage and anthraquinone residues when …

The Evolutionary Consequences Of Human–Wildlife Conflict In Cities, Christopher J. Schell, Lauren Stanton, Julie K. Young, Lisa Angeloni, Joanna E. Lambert, Stewart W. Breck, Maureen H. Murray

United States Department of Agriculture Wildlife Services: Staff Publications

Human–wildlife interactions, including human–wildlife conflict, are increasingly common as expanding urbanization worldwide creates more opportunities for people to encounter wildlife. Wildlife–vehicle collisions, zoonotic disease transmission, property damage, and physical attacks to people or their pets have negative consequences for both people and wildlife, underscoring the need for comprehensive strategies that mitigate and prevent conflict altogether. Management techniques often aim to deter, relocate, or remove individual organisms, all of which may present a significant selective force in both urban and nonurban systems. Managementinduced selection may significantly affect the adaptive or nonadaptive evolutionary processes of urban populations, yet few studies explicate the …

Modeling Mongoose Rabies In The Caribbean: A Model-Guided Fieldwork Approach To Identify Research Priorities, Caroline C. Sauvé, Erin E. Rees, Amy T. Gilbert, Are R. Berentsen, Agathe Allibert, Patrick A. Leighton

United States Department of Agriculture Wildlife Services: Staff Publications

We applied the model-guided fieldwork framework to the Caribbean mongoose rabies system by parametrizing a spatially-explicit, individual-based model, and by performing an uncertainty analysis designed to identify parameters for which additional empirical data are most needed. Our analysis revealed important variation in output variables characterizing rabies dynamics, namely rabies persistence, exposure level, spatiotemporal distribution, and prevalence. Among epidemiological parameters, rabies transmission rate was the most influential, followed by rabies mortality and location, and size of the initial infection. The most influential landscape parameters included habitat-specific carrying capacities, landscape heterogeneity, and the level of resistance to dispersal associated with topography. Movement …

Serological Responses Of Raccoons And Striped Skunks To Ontario Rabies Vaccine Bait In West Virginia During 2012–2016, Shylo R. Johnson, Dennis Slate, Kathleen M. Nelson, Amy J. Davis, Samual A. Mills, John T. Forbes, Kurt Vercauteren, Amy T. Gilbert, Richard B. Chipman

United States Department of Agriculture Wildlife Services: Staff Publications

Since the 1990s, oral rabies vaccination (ORV) has been used successfully to halt the westward spread of the raccoon rabies virus (RV) variant from the eastern continental USA. Elimination of raccoon RV from the eastern USA has proven challenging across targeted raccoon (Procyon lotor) and striped skunk (Mephitis mephitis) populations impacted by raccoon RV. Field trial evaluations of the Ontario Rabies Vaccine Bait (ONRAB) were initiated to expand ORV products available to meet the rabies management goal of raccoon RV elimination. This study describes the continuation of a 2011 trial inWest Virginia. Our objective was to evaluate raccoon …

Avian Influenza A Viruses Reassort And Diversify Differently In Mallards And Mammals, Ketaki Ganti, Anish Bagga, Juliana Dasilva, Samuel S. Shepard, John R. Barnes, Susan A. Shriner, Katia Koelle, Anice C. Lowen

United States Department of Agriculture Wildlife Services: Staff Publications

Reassortment among co-infecting influenza A viruses (IAVs) is an important source of viral diversity and can facilitate expansion into novel host species. Indeed, reassortment played a key role in the evolution of the last three pandemic IAVs. Observed patterns of reassortment within a coinfected host are likely to be shaped by several factors, including viral load, the extent of viral mixing within the host and the stringency of selection. These factors in turn are expected to vary among the diverse host species that IAV infects. To investigate host differences in IAV reassortment, here we examined reassortment of two distinct avian …

European Starling Nest‐Site Selection Given Enhanced Direct Nest Predation Risk, Bradley Blackwell, Thomas W. Seamans, Morgan Pfeiffer, Bruce N. Buckingham

United States Department of Agriculture Wildlife Services: Staff Publications

The European starling (Sturnus vulgaris) is recognized, outside its native range, as an invasive species, and efforts to deter its nesting have generally been unsuccessful. Enhancing perceived risk at the nest site is a little‐explored route in developing a nest deterrent. Specifically, nest predation risk affects site selection, reproduction, antipredator behavior, and fitness in nesting birds. We questioned whether perceived predation risk as related to a snake model positioned inside a nest box, in striking form, moving, and present throughout the period of cavity site selection would induce European starlings to avoid or delay nest construction. We conducted our study …

Best Management Practices For Trapping Furbearers In The United States, H. Bryant White, Gordon R. Batcheller, Edward K. Boggess, Clifford L. Brown, Joseph W. Butfiloski, Thomas A. Decker, John D. Erb, Michael W. Fall, David A. Hamilton, Tim L. Hiller, George F. Hubert Jr., Matthew J. Lovallo, John F. Olson, Nathan M. Roberts

United States Department of Agriculture Wildlife Services: Staff Publications

Humans have used wild furbearers for various purposes for thousands of years. Today, furbearers are sustainably used by the public for their pelts, leather, bones, glands, meat, or other purposes. In North America, contemporary harvest of furbearers has evolved along with trap technologies and societal concerns, and is now highly regulated and more closely coupled with harvest analysis and population monitoring. Traps and regulated trapping programs provide personal or cultural rewards that can also support conservation, and can assist with advancing ecological knowledge through research, protecting endangered species, restoring populations or habitats, protecting personal property, and enhancing public health and …

Variation In Reversal Learning By Three Generalist Mesocarnivores, Lauren A. Stanton, Eli S. Bridge, Joost Huizinga, Shylo R. Johnson, Julie K. Young, Sarah Benson‑Amram

United States Department of Agriculture Wildlife Services: Staff Publications

Urbanization imposes novel challenges for wildlife, but also provides new opportunities for exploitation. Generalist species are commonly found in urban habitats, but the cognitive mechanisms facilitating their successful behavioral adaptations and exploitations are largely under-investigated. Cognitive flexibility is thought to enable generalists to be more plastic in their behavior, thereby increasing their adaptability to a variety of environments, including urban habitats. Yet direct measures of cognitive flexibility across urban wildlife are lacking. We used a classic reversal-learning paradigm to investigate the cognitive flexibility of three generalist mesocarnivores commonly found in urban habitats: striped skunks (Mephitis mephitis), raccoons ( …

Social Identity And Acceptability Of Wild Pig (Sus Scrofa) Control Actions: A Case Study Of Texas Hunters, Lauren Jaebker, Tara L. Teel, Alan D. Bright, Hailey E. Mclean, John M. Tomeček, Maureen G. Frank, Rachael L. Connally, Stephanie A. Shwiff, Keith M. Carlisle

United States Department of Agriculture Wildlife Services: Staff Publications

Wild pigs (Sus scrofa) pose significant challenges to wildlife managers. This research explored Texas hunters’ acceptability of wild pig control actions, and whether acceptability varied according to hunters’ affiliation with four different categories of natural resource organizations as an indicator of social identity. Results of a survey (n = 37,317) revealed that most hunters were accepting of all control actions except toxicants and non-lethal deterrents. Mean acceptability scores for each action differed significantly across the four affiliation categories, but effect sizes were minimal. Hunters affiliated with agricultural organizations were the most accepting of control actions, while hunters with …

Strategic Considerations For Invasive Species Managers In The Utilization Of Environmental Dna (Edna): Steps For Incorporating This Powerful Surveillance Tool, Jeffrey Morisette, Stanley Burgiel, Kelsey Brantley, Wesley M. Daniel, John Darling, Jeanette Davis, Thomas Franklin, Keith Gaddis, Margaret Hunter, Richard Lance, Tracy Leskey, Yale Passamaneck, Antoinette Piaggio, Brian Rector, Adam Sepulveda, Melissa Smith, Carol A. Stepien, Taylor Wilcox

United States Department of Agriculture Wildlife Services: Staff Publications

Invasive species surveillance programs can utilize environmental DNA sampling and analysis to provide information on the presence of invasive species. Wider utilization of eDNA techniques for invasive species surveillance may be warranted. This paper covers topics directed towards invasive species managers and eDNA practitioners working at the intersection of eDNA techniques and invasive species surveillance. It provides background information on the utility of eDNA for invasive species management and points to various examples of its use across federal and international programs. It provides information on 1) why an invasive species manager should consider using eDNA, 2) deciding if eDNA can …

Reproductive Trade-Offs In The Colorado Checkered Whiptail Lizard (Aspidoscelis Neotesselatus): An Examination Of The Relationship Between Clutch And Follicle Size, Hannah E. Caracalas, S. S. French, S. B. Hudson, B. M. Kluever, A. C. Webb, D. Eifler, A. J. Lehmicke, Lise M. Aubry

United States Department of Agriculture Wildlife Services: Staff Publications

Life history theory predicts that there should be an inverse relationship between offspring size and number, because individuals cannot simultaneously maximize both when resources are limited. Although extensively studied in avian species, the occurrence and determinants of reproductive tradeoffs in oviparous reptiles are far less understood, particularly in parthenogenetic species. We studied this trade-off in the Colorado Checkered Whiptail, Aspidoscelis neotesselatus, a female-only parthenogenetic lizard. Using data previously collected in 2018 and 2019, we tested for clutch and egg size trade-offs and determined whether this relationship could be influenced by female size and aspects of physiological condition. Physiological condition …

Influenza A Virus Surveillance, Infection And Antibody Persistence In Snow Geese (Anser Caerulescens), Susan A. Shriner, J. Jeffrey Root, Jeremy W. Ellis, Kevin T. Bentler, Kaci K. Vandalen, Thomas Gidlewski, Sarah N. Bevins

United States Department of Agriculture Wildlife Services: Staff Publications

Some snow geese (Anser caerulescens) migrate between Eurasia and North America and exhibit high seroprevalence for influenza A viruses (IAVs). Hence, these birds might be expected to play a role in intercontinental dispersal of IAVs. Our objective in this manuscript was to characterize basic incidence and infection characteristics for snow geese to assess whether these birds are likely to significantly contribute to circulation of IAVs. Thus, we 1) estimated snow goose infection prevalence by summarizing > 5,000 snow goose surveillance records, 2) experimentally infected snow geese with a low pathogenic IAV (H4N6) to assess susceptibility and infection dynamics and …

Invasive Species In Puerto Rico: The View From El Yunque, Jess Zimmerman, Julissa Rojas-Sandoval, Aaron B. Shiels

United States Department of Agriculture Wildlife Services: Staff Publications

Native flora and fauna of Puerto Rico have a long biogeographic connection to South America. Theory and empirical evidence suggest that islands, particularly those distantly isolated from the mainland, should be more susceptible to naturalizations and invasions of non-native species than continental areas. Anthropogenic disturbances can facilitate accidental and deliberate introductions of non-native species. In this study, we asked: What is the current status of introduced species within El Yunque National Forest (EYNF), the largest and most well-conserved forest area of Puerto Rico? To address this question, we reviewed the literature and surveyed local experts to identify introduced plant and …

Modelling The Factors Affecting The Probability For Local Rabies Elimination By Strategic Control, Johann L. Kotzé, John Duncan Grewar, Aaron M. Anderson

United States Department of Agriculture Wildlife Services: Staff Publications

Dog rabies has been recognized from ancient times and remains widespread across the developing world with an estimated 59,000 people dying annually from the disease. In 2011 a tri-partite alliance consisting of the OIE, the WHO and the FAO committed to globally eliminating dog-mediated human rabies by 2030. Regardless of global support, the responsibility remains with local program managers to implement successful elimination programs. It is well known that vaccination programs have a high probability of successful elimination if they achieve a population-coverage of 70%. It is often quoted that reducing population turnover (typically through sterilizations) raises the probability for …

Continental-Scale Dynamics Of Avian Influenza In U.S. Waterfowl Are Driven By Demography, Migration, And Temperature, Erin E. Gorsich, Colleen T. Webb, Andrew A. Merton, Jennifer A. Hoeting, Ryan S. Miller, Matthew Farnsworth, Seth R. Swafford, Thomas J. Deliberto, Kerri Pedersen, Alan B. Franklin, Robert G. Mclean, Kenneth R. Wilson, Paul Doherty

United States Department of Agriculture Wildlife Services: Staff Publications

Emerging diseases of wildlife origin are increasingly spilling over into humans and domestic animals. Surveillance and risk assessments for transmission between these populations are informed by a mechanistic understanding of the pathogens in wildlife reservoirs. For avian influenza viruses (AIV), much observational and experimental work in wildlife has been conducted at local scales, yet fully understanding their spread and distribution requires assessing the mechanisms acting at both local, (e.g., intrinsic epidemic dynamics), and continental scales, (e.g., long-distance migration). Here, we combined a large, continental-scale data set on low pathogenic, Type A AIV in the United States with a novel network-based …

Avian Use Of Exotic Street Treescapes In Metropolitan Areas Of Phoenix, Arizona, Brian E. Washburn, Karen Hoss, David L. Bergman

United States Department of Agriculture Wildlife Services: Staff Publications

Urban areas are highly modified environments that are strongly influenced by a variety of anthropogenic factors. Consequently, these areas contain unique wildlife communities typically dominated by species that are generalist in nature or highly adaptable. We examined the use of five species of exotic treescapes by exotic and native birds in metropolitan areas of Phoenix, Arizona. House Sparrows [Passer domesticus (37%)], European Starlings [Sturnus vulagris (27%)], Mourning Doves [Zenaida macroura (11%)], and Great-tailed Grackles [Quiscalus mexicanus (7%)] were the most frequently observed species during the study. Approximately two-thirds (67%) of the birds observed during the study were exotic species. Avian …

A Sonic Net Reduces Damage To Sunflower By Blackbirds (Icteridae): Implications For Broad-Scale Agriculture And Crop Establishment, Amanda K. Werrell, Page E. Klug, Romuald N. Lipcius, John P. Swaddle

United States Department of Agriculture Wildlife Services: Staff Publications

Blackbirds, such as red-winged blackbirds (Agelaius phoeniceus), are notorious agricultural pests and damage crops at multiple stages of growth. Our aim was to test a novel deterrent, the use of sound designed to mask communication among birds (termed a “Sonic Net”), to deter blackbirds (Icteridae) from target areas of maturing sunflower crops. The Sonic Net masks communication of a target species by delivering “pink noise” that overlaps with the frequencies that the species uses for acoustic communication. If birds cannot hear predators or conspecific warning calls their perceived predation risk increases, and they relocate to an area with …

Surgical Sterilization Impacts On Behavior Of Coyote Pairs, Tyler Leary, Jeffrey T. Schultz, Julie K. Young

United States Department of Agriculture Wildlife Services: Staff Publications

Coyotes (Canis latrans) involved in depredation of livestock, an act frequently resulting in human-wildlife conflict, often do so out of necessity for provisioning pups. Surgical sterilization methods such as vasectomy that preserve gonadal hormones have been successful in reducing depredation by free-ranging coyotes while allowing individuals to maintain territoriality and mate fidelity. However, use of these methods remain costly and ineffective for wide-scale use. Given the alternative proposal of using chemical sterilization techniques, we investigated whether the use of hormone-altering sterilization methods impacted behavior of captive coyote pairs (i.e., male-female pair bonds). Our objective was to evaluate behavior …

Estimation Of Wildlife Damage From Federal Crop Insurance Data, Sophie Mckee, Stephanie A. Shwiff, Aaron M. Anderson

United States Department of Agriculture Wildlife Services: Staff Publications

BACKGROUND: Wildlife damage to crops is a persistent and costly problem for many farmers in the USA. Most existing estimates of crop damage have relied on direct assessment methods such as field studies conducted by trained biologists or surveys distributed to farmers. In this paper, we describe a new method of estimating wildlife damage that exploits federal crop insurance data. We focused our study on four crops: corn, soybean, wheat, and cotton, chosen because of their economic importance and their vulnerability to wildlife damage.

RESULTS: We determined crop-raiding hot spots across the USA over the 2015–2019 period and identified the …

A Framework For Surveillance Of Emerging Pathogens At The Human-Animal Interface: Pigs And Coronaviruses As A Case Study, Kim M. Pepin, Ryan S. Miller, Mark Q. Wilber

United States Department of Agriculture Wildlife Services: Staff Publications

Pigs (Sus scrofa) may be important surveillance targets for risk assessment and risk-based control planning against emerging zoonoses. Pigs have high contact rates with humans and other animals, transmit similar pathogens as humans including CoVs, and serve as reservoirs and intermediate hosts for notable human pandemics. Wild and domestic pigs both interface with humans and each other but have unique ecologies that demand different surveillance strategies. Three fundamental questions shape any surveillance program: where, when, and how can surveillance be conducted to optimize the surveillance objective? Using theory of mechanisms of zoonotic spillover and data on risk factors, …

Diets Of Double-Crested Cormorants In The Lake Winnebago System, Wisconsin, Ryan P. Koenigs, Daniel J. Dembkowski, Charles D. Lovell, Daniel A. Isermann, Adam D. Nickel

United States Department of Agriculture Wildlife Services: Staff Publications

Double-crested cormorant Phalacrocorox auritus Lesson (cormorant) populations have increased throughout the Great Lakes region of North America causing concern related to the impact of cormorant predation on fish communities. A recent decline in yellow perch Perca flavescens (Mitchill) abundance within the Lake Winnebago System, Wisconsin, USA, prompted an assessment of cormorant diets to evaluate potential effects of cormorant predation on the sportfish community. Diets were collected from 883 cormorants (417 from Lake Winnebago and 466 from Lake Butte des Morts) between 2015 and 2017. Cormorant diets on both waterbodies consisted mostly of freshwater drum Aplodinotus grunniens Rafinesque and gizzard shad …

Evaluation Of A Warfarin Bait For Controlling Invasive Wild Pigs (Sus Scrofa), James C. Beasley, Lindsay M. Clontz, Allison Rakowski, Nathan P. Snow, Kurt C. Vercauteren

United States Department of Agriculture Wildlife Services: Staff Publications

BACKGROUND: Wild pigs (Sus scrofa) cause widespread environmental and economic damage, and as a result are subjected to extensive control. Current management strategies have proven insufficient, and there is growing interest in use of toxicants to control invasive populations of this species. In 2017 a low-dose warfarin bait was federally approved for use in controlling wild pigs in the United States. However, no states have allowed use of this bait due to unanswered questions regarding welfare concerns, field efficacy, and non-target impacts.

RESULTS: All captive wild pigs fed 0.005% warfarin baits in no choice feeding trials succumbed in an average …

Space Use In Free-Ranging Canids: Are Gonadal Hormones Required For Territory Maintenance?, Eric M. Gese, Patricia A. Terletzky

United States Department of Agriculture Wildlife Services: Staff Publications

Fertility control among carnivores has been used to reduce depredations on livestock and wild neonates, population control, modify behavior, inhibit genetic introgression, and reduce human–wildlife conflicts. Although there is considerable knowledge on techniques to sterilize carnivores, there is little information concerning how the absence of gonadal hormones influences behavior, space use, and survival of wild canids. We examined territorial fidelity, home-range size and overlap, and survival of 179 surgically sterilized free-ranging canids (124 coyotes (Canis latrans Say, 1823), 55 coyote – red wolf (Canis rufus Audubon and Bachman, 1851) hybrids) with gonadal hormones present (tubal-ligated females (n = 70), vasectomized …

Estimating Wildlife Strike Costs At Us Airports: A Machine Learning Approach, Levi Altringer, Jordan Navin, Michael J. Begier, Stephanie A. Shwiff, Aaron M. Anderson

United States Department of Agriculture Wildlife Services: Staff Publications

Current lower bound estimates of the economic burden of wildlife strikes make use of mean cost assignment to impute missing values in the National Wildlife Strike Database (NWSD). The accuracy of these estimates, however, are undermined by the skewed nature of reported cost data and fail to account for differences in observed strike characteristics—e.g., type of aircraft, size of aircraft, type of damage, size of animal struck, etc. This paper makes use of modern machine learning techniques to provide a more accurate measure of the strike-related costs that accrue to the US civil aviation industry. We estimate that wildlife strikes …

Viral And Immune Studies On Bovine Viral Diarrhea Virus (Bvdv) In Bovine Species, Karim Abdelsalam

Electronic Theses and Dissertations

BVDV is a major pathogen of ruminants that causes immune dysfunction and lead to secondary bacterial infection. The underlying mechanism of this dysfunction is not well understood. Based on previous results, we believe that macrophages play a central role in BVDV-associated immune dysfunction. Previously, we have shown that the infected monocyte-derived macrophage (MDM) supernatant of the high virulent strains of BVDV significantly induced lymphocyte apoptosis. In the current study, we performed experiments to identify the apoptotic factors in the infected MDM supernatants in vitro. Also, we have extended our investigation to include testing the impact of infected macrophages on neutrophil …

Evidence For Continental-Scale Dispersal Of Antimicrobial Resistant Bacteria By Landfill-Foraging Gulls, Christina A. Ahlstrom, Mariëlle L. Van Toor, Hanna Woksepp, Jeffrey C. Chandler, John A. Reed, Andrew B. Reeves, Jonas Waldenström, Alan B. Franklin, David C. Douglas, Jonas Bonnedahl, Andrew M. Ramey

United States Department of Agriculture Wildlife Services: Staff Publications

Anthropogenic inputs into the environment may serve as sources of antimicrobial resistant bacteria and alter the ecology and population dynamics of synanthropic wild animals by providing supplemental forage. In this study, we used a combination of phenotypic and genomic approaches to characterize antimicrobial resistant indicator bacteria, animal telemetry to describe host movement patterns, and a novel modeling approach to combine information fromthese diverse data streams to investigate the acquisition and long-distance dispersal of antimicrobial resistant bacteria by landfill-foraging gulls. Our results provide evidence that gulls acquire antimicrobial resistant bacteria from anthropogenic sources, which they may subsequently disperse across and between …