Veterinary Microbiology and Immunobiology Commons^™

Monoclonal Antibodies To The Fusion Protein Of Bovine Respiratory Syncytial Virus, Kent M. Mulkey, Gary A. Anderson

School of Veterinary and Biomedical Sciences: Faculty Publications

Five monoclonal antibodies specific for bovine respiratory syncytial virus were characterized by Western immunoblotting, radioimmunoprecipitation, and epitope mapping assays. The monoclonal antibodies were found to be specific for the fusion protein, and there were at least two antigen binding sites, one of which was neutralizing.

Replication And Amplification Of Defective Interfering Particle Rn As Of Vesicular Stomatitis Virus In Cells Expressing Viral Proteins From Vectors Containing Cloned Cdnas, Asit K. Pattnaik, Gail W. Wertz

School of Veterinary and Biomedical Sciences: Faculty Publications

Replication and amplification of RNA genomes of defective interfering (DI) particles of vesicular stomatitis virus (VSV) depend on the expression of viral proteins and have untD now been attained only in ceUs coinfected with helper VSV. In the work described in this report, we used a recombinant vaccinia virus-T7 RNA polymerase expression system to synthesize individual VSV proteins in cells transfected with plasmid DNAs that contain cDNA copies of the VSV genes downstream of the T7 RNA polymerase promoter. In this way, we were able to examine the ability of VSV proteins, individually and in combination, to support DI particle …

Efficacy Of A Cell Extract From Actinobacillus (Haemophilus) Pleuropneumoniae Serotype 1 Against Disease In Swine, P. J. Fedorka-Cray, M.J. Huether, D.L. Stine, G. A. Anderson

School of Veterinary and Biomedical Sciences: Faculty Publications

We partially characterized a cell extract (CE) from Actinobacillus pleuropneumoniae serotype 1 and used the CE to test the efficacy of secreted proteins against disease. Secreted products from 4-h culture supernatants were precipitated with 20% polyethylene glycol. Analysis of the CE indicated the presence of protein, endotoxin, and carbohydrate. Hemolytic activity to bovine erythrocytes and cytotoxic activity to porcine mononuclear leukocytes was also demonstrated. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of the CE from a 4-h culture showed a major band at 110 kilodaltons (kDa), while a CE of a 26-h culture indicated the presence of a number of additional …

Acceptance Of Simulated Oral Rabies Vaccine Baits By Urban Raccoons, John Hadidian, Suzanne R. Jenkins, David H. Johnston, Peter J. Savarie, Victor F. Nettles, David M. Manski, George M. Baer

Veterinary Science and Medicine Collection

In summer 1986, a study was conducted to evaluate raccoon (Procyon lotor) acceptance of oral baits that could be used for rabies vaccination, One thousand wax-coated sponge bait cubes were filled with 5 mg of a seromarker (iophenoxic acid), placed in polyethylene bags, and hand-distributed in an 80 ha area within an urban National Park in Washington, D.C. (USA), After 3 wk, target and nontarget animals were trapped and blood samples collected to evaluate bait uptake. Thirty-three of 52 (63%) raccoons had elevated blood iodine levels indicating they had eaten at least one bait, 13 (25%) were negative, and six …

Improved Selective Medium For The Isolation Of Treponema Hyodysenteriae, Robert A. Kunkle, J. M. Kinyon

School of Veterinary and Biomedical Sciences: Faculty Publications

An agar medium with improved selection for Treponema hyodysenteriae was developed. Cultures of T. hyodysenteriae and T. innocens, feces from 11 clinically normal pigs, and colonic contents from 6 pigs with gross lesions consistent with swine dysentery were diluted in phosphate-buffered saline and plated on Trypticase soy agar (BBL Microbiology Systems, Cockeysville, Md.) with 5% citrated bovine blood (TSA), TSA with 400 ,ug of spectinomycin per ml (TSA-S400), TSA-S400 with 25 ,ug each of colistin and vancomycin per ml, and TSA with 5% pig feces extract and five antimicrobial agents (spiramycin, rifampin, vancomycin, colistin, and spectinomycin) (BJ). Viable numbers …

Formation Of Influenza Virus Particles Lacking Hemagglutinin On The Viral Envelope, Asit K. Pattnaik, Donald J. Brown, Debi P. Nayak

School of Veterinary and Biomedical Sciences: Faculty Publications

We investigated the intraceUular block in the transport of hemagglutinin (HA) and the role of HA in virus particle formation by using temperature-sensitive (Is) mutants (1s134 and 1s61S) of inOuenza virus AlWSN/33. We found that at the nonpermissive temperature (39.5°C), the exit of ts HA from the rough endoplasmic reticulum to the Golgi complex was blocked and that no additional block was apparent in either the exit from the Golgi complex or post-Golgi complex transport. When MDBK ceUs were infected with these mutant viruses, they produced noninfectious virus particles at 39.5°C. The efficiency of particle formation at 39.5°C was essentiaUy …

'Dermo' Fleece Rot And Body Strike On Sheep, S G. Gherardi, S. S. Sutherland, N. Monzu

Journal of the Department of Agriculture, Western Australia, Series 4

Blowfly strike is one of the major problems confronting the sheep industry in Austrsalia, with an estimated totsalcost of control eceeding $100 million each year.

Sheep are susceptible to five types of strike: body, breach, poll, pizzle and wound. Body strike, is of greater economic importance because its sporadic occurence from year to year makes it difficult to predict and cintrol. Outbreaks of body strike after rain can affect large numbers of sheep and inflict severe production losses.

In eastern Australia fleece rot is generally considered to be the main predisposing factor to blowfly strike, but in Western Australia evidence …

New Developments In Footrot Control, R B. Richards, L. J. Depiazzi, R. V. R. Gwynn

Journal of the Department of Agriculture, Western Australia, Series 4

The eradication policy for footrot in sheep adopted by the Western Australian Department of Agriculture has produced an overall decline in the number of properties with the disease.

Our knowledge of the disease has improved substantially as a result of the research conducted at the Slabany Regional Veterinary Laboratory in recent years. Laboratory tests now sssist stock inspectors and veterinary officers to distinguish between different forms of footrot and to quarantine and eradicate accordingly.

The recent discovery of more effective foot-bathing solutions by officers of the Victorian Department of Agriculture will greatly assist further eradication procedures.

Western Australia's sheep industry …

Identification Of Four Complementary Rna Species In Akabane Virus-Infected Cells, Asit K. Pattnaik, G. Abraham

School of Veterinary and Biomedical Sciences: Faculty Publications

The analysis of RNA extracted from purified Akabane virus demonstrated the presence of three size classes of single-stranded RNAs with sedimentation coefficients of 31S (large, L), 26S (medium, M), and 13S (small, S). Molecular weights of these RNA species were estimated to be 2.15 x 10⁶,1.5 X 10⁶, and 0.48 x 10⁶ for the L, M, and S RNAs, respectively. Hybridization analysis involving viral genomic RNA and RNA from virus-infected cells resulted in the identification of four virus-specific cRNA species in infected cells. These cRNAs were found to be nonpolyadenylated by their inability to bind …

Mycotic Dermatitis (Lumpy Wool) Of Sheep, M R. Gardiner

Journal of the Department of Agriculture, Western Australia, Series 4

MYCOTIC dermatitis is a common disease of the skin and overlying wool of sheep (and other herbivorous animals).

The photographs show the typical crusts and scabs which result from infection of skin, hair and wool follicles by the mycotic dematitis organism.

This article reviews the causes of lumpy wool infection and sets out control measures.

Infectious Sinusitis Of Turkeys, J Edgar

Journal of the Department of Agriculture, Western Australia, Series 4

Climatic conditions in W.A. are ideal for turkey production if advantage is taken of the wide open spaces. Over-crowded, poorly ventilated quarters are ideal for the spread of infectious sinusitis, and the disease, once established, is hard to eliminate without a complete change of birds.

This article provides advice for the prevention of this important disease at a time when turkey raising is increasing in W.A.

Calf Diarrhea (Scours): Reproduced With A Virus From A Field Outbreak, C. A. Mebus, N. R. Underdahl, M. B. Rhodes, M. J. Twiehaus

Nebraska Agricultural Experiment Station: Historical Research Bulletins

Neonatal calf diarrhea typical of that seen in field cases was produced in colostrum-deprived calves by inoculating either feces or bacteria-free filtrates via the duodenum, orally, or by a spray (aerosol). Three calves, kept free of E. coli, developed severe diarrhea following inoculation with a bacteria-free filtrate. Intense fluorescence in the epithelium of sections of small intestine from two experimental calves was observed following staining with ftuorescein-labeled gamma globulin produced with viral antigen prepared from the feces of two experimental diarrhetic E. coli-free calves. Fluorescing cells were also present in the feces of experimental and field cases of neonatal …

Bluetongue Of Sheep, M R. Gardiner

Journal of the Department of Agriculture, Western Australia, Series 4

BLUETONGUE is a serious disease of sheep, mainly of introduced European breeds, throughout the African continent.

It is maintained there by wild and domestic ruminants in the presence of certain insect vectors (carriers).

The disease has probably existed there from immemorial times among the indigenous ruminants, emerging as a recognised disease only when the much more susceptible European breeds were brought into the continent.

Feeding Urea To Dairy Cows, R A. Bettenay

Journal of the Department of Agriculture, Western Australia, Series 4

IT has been known for some years that the ruminant animal, through the agency of microflora in the rumen, has the ability to use urea as a source of nitrogen, and to convert some of this to protein.

Enterotoxaemia (Pulpy Kidney Disease), J Craig

Journal of the Department of Agriculture, Western Australia, Series 4

ENTEROTOXAEMIA, or pulpy kidney disease, is found in all areas of the world where sheep are raised and has occurred in Western Australia for many years, particularly in the great southern districts.

At one time it was known as "Beverley sheep disease."

The infectious nature of enterotoxaemia was first described by Bennets in 1932.

Down And Out [Pulpy Kidney Disease], F C. Wilkinson

Journal of the Department of Agriculture, Western Australia, Series 4

Two days previously the weaner mob had broken through a gate into a hundred acre paddock in which an excellent green oat crop was growing.

The owner had not been particularly worried because the oats needed grazing. When however, he went to check the water troughs and found 17 weaners dead, he became perturbed.

Viral Infections Of Domestic Animals, George A. Young

School of Veterinary and Biomedical Sciences: Faculty Publications

There is a strong tendency in current research on mammalian virus diseases to concentrate on virus activity at a cellular or subcellular level. This trend is summarized adequately by Enders in relation to viral replication as follows: ','The large and ever-increasing volume of published experimental work on viral replication strikingly reveals the central position of this phenomenon in contemporary virological research. There are good reasons, both biological and practical, underlying this intensive effort to understand, i n detail, how a virus particle, without energy transforming apparatus of its own, manages to utilize the metabolic equipment of the cell to produce …

Worm Eggs: Cost You Money, E.F. Frolik, E.W. Janike

School of Veterinary and Biomedical Sciences: Faculty Publications

Contents

One Million Eggs Daily.......... 3

Damaging Trip......... 4

Worm Remedies......... 5

Modern Worm Remedies......... 6

A Control Program.......... 8

Antibiotics And Sterilisers In The Dairy Industry, K Needham, N. Ingleton

Journal of the Department of Agriculture, Western Australia, Series 4

A S a consequence of the rapid advances made in all aspects of technology there are an ever increasing number of preparations becoming available to the dairy farmer, to assist both in the control of disease in his crops, pastures and animals and to maintain and enhance the quality of his dairy products.

These are—antibiotics, sterilisers, detergents, various weedicides and pesticides

Antibiotic Residues In Milk : The Farmer's Responsibility, N Ingleton

Journal of the Department of Agriculture, Western Australia, Series 4

THE LEVEL of antibiotic residues in commercial milk supplies has been steadily increasing over the past few years.

These residues find their way into the bulk milk as a result of milk from cows under antibiotic treatment for mastitis or other infections being included in the bulk milk for sale.

Pinkeye In Sheep : Some Timely Comments, F C. Wilkinson

Journal of the Department of Agriculture, Western Australia, Series 4

DURING the summer months, it is almost inevitable that numbers of sheep in Western Australia will suffer from contagious ophthalmia—or pinkeye, to give the disease its more popular name.

As it can be quite a distressing disease which can cause severe setbacks, most flock owners, knowing that medicaments are available for the treatment of pinkeye, will feel that they should do something for the affected animals.

Pulpy Kidney Is Still A Sheep Killer, F C. Wilkinson

Journal of the Department of Agriculture, Western Australia, Series 4

ALTHOUGH considerable publicity has been given to methods of control by vaccination, numbers of sheep are lost every year in outbreaks of infectious enterotoxaemia— the disease commonly known as "pulpy kidney."

Histopathology Of Bovine Mastitis, The, C.F. Helmboldt, E.L. Jungherr, W.N. Plastridge

Storrs Agricultural Experiment Station

No abstract provided.

Studies On Nutritional Muscular Dystrophy, I. Dietary Factors, Ii. Fibrosis And Lipomatosis Of Tissues, Violet Myrtle Wilder

Department of Biochemistry: Dissertations, Theses, and Student Research

dissertation published as three peer-reviewed papers:

Sergius Morgulis, Violet M. Wilder, and S. H. Eppstein. (September 1938). Further studies on dietary factors associated with nutritional muscular dystrophy. Journal of Nutrition 16(3): 219-227.

Howard C. Spencer, Sergius Morgulis, and Violet M. Wilder. (August 1937). A micromethod for the determination of gelatin and a study of the collagen content of muscles from normal and dystrophic rabbits. Journal of Biological Chemistry 120(1): 257-266.

Sergius Morgulis, Violet M. Wilder, Howard C. Spencer, and S. H. Eppstein. (August 1938). Studies on the lipid content of normal and dystrophic rabbits. Journal of Biological Chemistry 124(3): 755-766.

Studies Of Immunity Against Hemorrhagic Septicemia, L. Van Es, H. M. Martin

Nebraska Agricultural Experiment Station: Historical Research Bulletins

As one of the phases of our inquiry an effort was made to acquire some general knowledge regarding the immunizing value (1) of sera prepared with the aid of Bacillus bipolaris septicus procured from cattle and swine or (2) of those alleged to be of service in the control or treatment of hemorrhagic septicemia in the species mentioned. The experiments reported in this publication were almost exclusively made with sera purchased from dealers in biologic products.

The Corn-Stalk Disease In Cattle, Frank S. Billings

School of Veterinary and Biomedical Sciences: Faculty Publications

MORPHO-BIOLOGICAL CORRESPONDENCE BETWEEN TWO OR MORE MICRO- ETIOLOGICAL ORGANISMS NOT SUFFICIENT GROUNDS FOR PRONOUNCING THE DISEASES WITH WHICH THEY ARE CONNECTED IDENTICAL. The details of this discussion will be found in my report on the swine-plague. It is necessary, however, to touch upon the essential points here also. As was there shown, Hueppe asserts that the European diseases previously mentioned as being caused by a member of this group of belted, ovoid germs, viz., the “ Huhne Cholera, Kaninchen Septikasmie, und Wild Seuche ’ ’ are all one and the same disease, because their micro-etiological organisms have the same form, …

Keratitis Contagiosa In Cattle, Frank S. Billings

School of Veterinary and Biomedical Sciences: Faculty Publications

This is not a new disease by any means, so far as the United States are concerned, nevertheless I have been unable to find any description of it in the literature at my command. While new to myself until the past Summer, there have been quite a number of reports of its existence, and complaints about it, from farmers and breeders of cattle in some of the live-stock journals of our western States. Under these circumstances, it would seem that a description of its clinical phenomena and gross pathological lesions may not be without scientific interest to the opthalmologist, and …

Inoculation—Vaccination, Frank S. Billings

School of Veterinary and Biomedical Sciences: Faculty Publications

When one comes to discuss this question with the members of the profession as he casually meets them, he is often surprised to find how uncertain and clouded the prevailing ideas are upon the subject. In fact, the majority of physicians do not seem to have any clear conception of the act connected with the words “ inoculation ” and “ vaccination.”