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Veterinary Infectious Diseases Commons™
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- ELISA (2)
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- African horse sickness virus (AHSV) (1)
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- Antimicrobial susceptibility profiles (1)
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Articles 1 - 9 of 9
Full-Text Articles in Veterinary Infectious Diseases
Evaluation Of Chicken Serum And Chicken Egg Yolk As Novel Supplements For The In Vitro Cultivation Of Mycoplasma Hyorhinis, Yi-Chia Li, Sin-Hang Ma, Wei-Hao Lin, Chao-Nan Lin, Ming-Tang Chiou
Evaluation Of Chicken Serum And Chicken Egg Yolk As Novel Supplements For The In Vitro Cultivation Of Mycoplasma Hyorhinis, Yi-Chia Li, Sin-Hang Ma, Wei-Hao Lin, Chao-Nan Lin, Ming-Tang Chiou
The Thai Journal of Veterinary Medicine
Mycoplasma hyorhinis is a significant pathogen in swine, yet its fastidious nutritional requirements present challenges for in vitro cultivation. Conventional supplements like horse serum (HS) often suffer from batch variability and potential inhibitors. This study aimed to evaluate chicken serum (CS) and chicken egg yolk (CY) as novel alternatives to develop a reliable cultivation method for M. hyorhinis. Five clinical isolates were cultured for 10 days in a basal medium supplemented with CS or CY at 10%, 20%, or 30%, with 30% HS as the control. Growth was quantified daily using a Color Changing Unit (CCU) assay. CS supported …
Recovery And Characterization Of Moraxella Species From Bovine Specimens, Laura G. Leger
Recovery And Characterization Of Moraxella Species From Bovine Specimens, Laura G. Leger
School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research
The family Moraxellaceae is made up of a diverse group of 24 named species that fall into three phylogenetic groups. While most Moraxella spp. act as commensal respiratory and/or ocular flora of mammals, some are associated with disease. One of these diseases, infectious bovine keratoconjunctivitis (IBK), has significant welfare and economic impacts to both cattle and cattle producers. Moraxella bovis is the only experimentally determined cause of IBK, however, an association of Moraxella bovoculi with disease has been proposed. Currently, available vaccines do not have high levels of efficacy in the field. Culture based methods are the gold standard for …
Seroprevalence Of Small Ruminant Lentiviruses (Srlvs) And Peste Des Petits Ruminants Virus (Pprv) In Nonvaccinated Sheep And Goats In Sarawak, Malaysia, Bura Thlama Paul, Ali Saidu, Maria Eva Barudi, Faez Firdaus Abdullah Jesse, Juriah Kamaludeen, Suhaili Mustafa, Masnindah Malahubban, Ali Hanafiah Hakim, Krishnan Nair Balakrishnan, Mohd Azmi Mohd Lila
Seroprevalence Of Small Ruminant Lentiviruses (Srlvs) And Peste Des Petits Ruminants Virus (Pprv) In Nonvaccinated Sheep And Goats In Sarawak, Malaysia, Bura Thlama Paul, Ali Saidu, Maria Eva Barudi, Faez Firdaus Abdullah Jesse, Juriah Kamaludeen, Suhaili Mustafa, Masnindah Malahubban, Ali Hanafiah Hakim, Krishnan Nair Balakrishnan, Mohd Azmi Mohd Lila
The Thai Journal of Veterinary Medicine
Small ruminant lentiviruses (SRLVs) and Peste des Petits Ruminants virus (PPRV) are economically important viral diseases that threaten small ruminant production globally. While previous studies have reported their serological status in Peninsular Malaysia, there is limited epidemiological information on their occurrence in East Malaysia. This study aimed to determine the seroprevalence and risk factors of SRLVs and PPRV among nonvaccinated sheep and goats from selected smallholder farms in Sarawak. A total of 245 serum samples collected from sheep (n = 117) and goat (n = 128) were screened using commercial ID Screen® PPR competition (PPRC ver. 0821) and MVV/CAEV …
Molecular Identification Of Cryptosporidium Spp. Isolated From Dairy Calves In The Northern Region Of Algeria, Dadda Anes, Abdellah Mohamed Cherif, Aoun Leila, Chatanun Eamudomkarn, Khelef Djamel, Keeseon S Eom, Dongmin Lee, Barakaeli Abdieli Ndosi, Mohammed Mebarek Bia
Molecular Identification Of Cryptosporidium Spp. Isolated From Dairy Calves In The Northern Region Of Algeria, Dadda Anes, Abdellah Mohamed Cherif, Aoun Leila, Chatanun Eamudomkarn, Khelef Djamel, Keeseon S Eom, Dongmin Lee, Barakaeli Abdieli Ndosi, Mohammed Mebarek Bia
The Thai Journal of Veterinary Medicine
Cryptosporidium spp. is the most frequent agent in Algeria that causes neonatal diarrhea in calves, with high symptomatic diarrhea in pre-weaned dairy calves. The study aims to measure the prevalence, distribution, and genotype of Cryptosporidium-infected calves in one of Algeria's high milk production areas (North Central and Northeast). From 35 dairy cattle farms were selected 172 (77 with diarrheic and 95 without diarrheic symptoms) calves aged less than 60 days. Twenty-three farms (65.71%) were detected with cryptosporidiosis using a modified Ziehl-Neelsen stain, whereby 68 calves (39.53%) were obtained positive. The Copro-ELISA test gave an individual prevalence of (37.66%, 22.11%) …
Fetal Losses Associated With Prrsv, Ppv, And Pcv2 Detection, Em-On Olanratmanee, Pachara Pearodwong, Duangkamol Lewchalermwong, Padet Tummaruk
Fetal Losses Associated With Prrsv, Ppv, And Pcv2 Detection, Em-On Olanratmanee, Pachara Pearodwong, Duangkamol Lewchalermwong, Padet Tummaruk
The Thai Journal of Veterinary Medicine
This study aimed to investigate the prevalence of porcine reproductive and respiratory syndrome virus (PRRSV), porcine parvovirus (PPV), and porcine circovirus type 2 (PCV2) infections associated with fetal losses in 10 commercial swine herds in Thailand. Samples were collected from 100 sows and included 25 aborted fetuses, 35 mummified fetuses, and 40 stillborn piglets. The average parity number of the sows was 2.8 ± 2.4, ranging from 0 to 11. Fetal age was determined based on either the day of abortion or estimated using crown-rump length, and categorized as ≥70 days. Organs from each case were pooled, homogenized, and tested …
Antimicrobial Susceptibility Profiles (Mic And Mmc) Against Mycoplasma Gallisepticum And M. Synoviae Isolated From Chicken Farms In Thailand, Kriengwich Limpavithayakul, Alongkorn Amonsin, Nataya Charoenvisal, Arithat Limsatanun, Katanyu Yangsuk, Sumrit Toopiya, Füsun Güngör, Esther Schonewille, Javier Uriarte, Somsak Pakpinyo
Antimicrobial Susceptibility Profiles (Mic And Mmc) Against Mycoplasma Gallisepticum And M. Synoviae Isolated From Chicken Farms In Thailand, Kriengwich Limpavithayakul, Alongkorn Amonsin, Nataya Charoenvisal, Arithat Limsatanun, Katanyu Yangsuk, Sumrit Toopiya, Füsun Güngör, Esther Schonewille, Javier Uriarte, Somsak Pakpinyo
The Thai Journal of Veterinary Medicine
Mycoplasma gallisepticum (MG) and M. synoviae (MS) are important pathogens causing significant economic losses in poultry production. Disease prevention and control strategies include vaccination, antimicrobial therapy, and strict biosecurity measures. The present study provides, for the first time, both minimum inhibitory concentration (MIC) and minimum mycoplasmacidal concentration (MMC) values as antimicrobial susceptibility profiles of Thai MG and MS field isolates. This study aimed to evaluate the susceptibility of five MG and ten MS isolates to various antimycoplasma drugs: doxycycline, lincomycin-spectinomycin, tiamulin, tilmicosin, tylosin, and tylvalosin, using a microdilution broth assay. For MG isolates, tilmicosin had the highest mean MIC (12.5 …
Monitoring Of African Horse Sickness Virus-Specific Antibodies In Horses, Northeastern Thailand, Napawan Bunpapong, Kitikhun Udom, Ekkapat Chamsai, Patchara Phuektes, Suphattra Jittimanee, Suphannika Phutthachalee, Supassama Chaiyawong, Kamonpan Charoenkul, Alongkorn Amonsin Prof. Dr.
Monitoring Of African Horse Sickness Virus-Specific Antibodies In Horses, Northeastern Thailand, Napawan Bunpapong, Kitikhun Udom, Ekkapat Chamsai, Patchara Phuektes, Suphattra Jittimanee, Suphannika Phutthachalee, Supassama Chaiyawong, Kamonpan Charoenkul, Alongkorn Amonsin Prof. Dr.
The Thai Journal of Veterinary Medicine
African horse sickness (AHS) is caused by infection with the African horse sickness virus (AHSV). In March 2020, AHS was first reported in Thailand due to the importation of subclinically infected host animals, such as zebras. In this study, we conducted a serological survey for AHSV-specific antibodies across 21 horse farms in the northeastern provinces (n=3) of Thailand. A total of 600 serum samples were collected for cross-sectional (n = 292) and longitudinal serological monitoring (n = 308). A cross-sectional study was conducted in 2020, during which serum samples (n = 292) were collected and tested for AHS antibodies using …
Overcoming Vaccine-Associated Enhanced Respiratory Disease In Swine Flu With Lipid Nanoparticle-Dna Technology, The Nguyen
School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research
The two primary challenges in developing vaccines against swine influenza A virus (IAV-S) are the continuous viral evolution and vaccine-associated enhanced respiratory disease (VAERD). Current vaccine strategies share a common limitation: the significant time required for licensing updated vaccines. Moreover, the strain selection for vaccine updates depends entirely on predictions, raising the risk that chosen strains may not match circulating viruses. Such mismatches not only reduce vaccine efficacy but also risk exacerbating disease severity through VAERD. Here, in this thesis, we developed and evaluated a novel DNA-based vaccine platform designed to address the need for rapid updates in response to …
Correlation Of Classical Swine Fever (Csf) Antibody Protective Level Detected By Enzyme-Linked Immunosorbent Assay And Serum Neutralization Test, Chairani Ridha Maghfira, Nguyen Thanh Che, Apisit Kittawornrat, Roongroje Thanawongnuwech, Yaowalak Panyasing
Correlation Of Classical Swine Fever (Csf) Antibody Protective Level Detected By Enzyme-Linked Immunosorbent Assay And Serum Neutralization Test, Chairani Ridha Maghfira, Nguyen Thanh Che, Apisit Kittawornrat, Roongroje Thanawongnuwech, Yaowalak Panyasing
The Thai Journal of Veterinary Medicine
Protective antibody against classical swine fever virus (CSFV) is an important parameter used for disease monitoring in CSFV-endemic areas. Enzyme-linked immunosorbent assay (ELISA) is a simple and practical serological assay for CSFV antibody detection. However, the use of CSFV ELISAs in CSFV antibody-positive herds, i.e., CSFVvaccinated or -previously infected herds, was limited by an application of the test results. This study aimed to evaluate the correlation and estimation of protective antibody levels detected by ELISA and serum neutralization assay. A total of 522 negative and positive serums were tested by SN and ELISA in parallel. Comparisons of sample-to-positive (S/P) values …