Open Access. Powered by Scholars. Published by Universities.®

Veterinary Infectious Diseases Commons

Open Access. Powered by Scholars. Published by Universities.®

Articles 1 - 3 of 3

Full-Text Articles in Veterinary Infectious Diseases

Whole Genome Sequencing Of West Nile Viruses From Animals Submitted To The Connecticut Veterinary Medical Diagnostic Laboratory (Cvmdl), Usa, 2021, Allison Appel May 2023

Whole Genome Sequencing Of West Nile Viruses From Animals Submitted To The Connecticut Veterinary Medical Diagnostic Laboratory (Cvmdl), Usa, 2021, Allison Appel

Honors Scholar Theses

West Nile Virus (WNV) causes a zoonotic disease. WNV was first isolated in Uganda in 1937 and has since become an endemic in countries such as North, South, and Central America [1]. According to the Connecticut Department of Public Health, an average of 8 cases of WNV in humans have been reported since the year 2000 [2]. In this study, we focused on the genetic characterization of the viral genomic RNA of WNVs circulating in birds and mammals in the New England region. Following that aim, the complete genome sequence of two WNVs was obtained via next-generation sequencing. One sample …


Development Of Repressible Systems To Control Gene Expression In Vaccinia Virus, Allison Titong Aug 2012

Development Of Repressible Systems To Control Gene Expression In Vaccinia Virus, Allison Titong

Master's Theses

Two vaccinia virus (VACV) expression systems that contain elements from the lactose (lac) and the tetracycline (tet) operons of E. coli were developed to repress the expression of a reporter gene, enhanced green fluorescent protein (EGFP), in the presence of tet operon inducers. In the first system, lac and tet operon elements were arranged in a gene circuit, and in the presence of increasing concentrations of a lac operon inducer (isopropyl-β-D-thiogalactoside, IPTG), EGFP expression increased in a dose dependent manner and at high IPTG concentrations, expression reached the same levels as a positive control virus. Importantly, in the …


Raccoon Acclimation Towards Traps And Its Effect On Surveillance And Monitoring Of Zoonotic Diseases, Lindsay Rollend May 2012

Raccoon Acclimation Towards Traps And Its Effect On Surveillance And Monitoring Of Zoonotic Diseases, Lindsay Rollend

Master's Theses

Raccoons (Procyon lotor) serve as a source of infection with various pathogens for people, most notably, rabies. Raccoons are specifically targeted for the control of rabies in the United States, primarily due to the 100% fatality rate in humans and animals. There is no cure or treatment for rabies and vaccines may be administered to people if it is administered promptly. In order to evaluate how successful trapping of raccoons is by using conventional methods, motion sensing cameras were placed on traps in order to captured raccoon behaviors. Pictures were coded based on these behaviors (around the trap but not …