Veterinary Anatomy Commons^™

The Effects Of Insulin On Hepatic Glucocorticoid Receptor Content In The Diabetic Rat, Jeffrey John Yourick

Masters Theses

The streptozotocin-induced diabetic rat liver was analyzed for glucocorticoid receptor (GCR) content by a saturation and Scatchard analysis. A preliminary study was performed on the stabilization of the cytoplasmic GCR in a frozen cytosol preparation. The addition of 10% glycerol (V/V), 10 mM sodium molybdate, and 0.2 mg/ml trypsin inhibitor resulted in an approximate 20% sparing of GCR content upon freezing.

The hepatic GCR content of diabetic rats was significantly decreased from a control level of 0.1667 ± 0.0142 pmol/mg protein to 0.1094 ± 0.007 pmol/mg protein. Insulin replacement therapy to the diabetic rat dramatically increased the hepatic GCR content. …

The Effects Of Prostaglandin E2 On The Healing Processes Of Rat Perforated Tympanic Membranes, Diane Sue Saylor

Masters Theses

Prostaglandin E₂ (PGE₂) was topically administered to perforated rat tympanic membranes in an attempt to facilitate regrowth of the membranes. Every 24 hours for the first four postperforation days, two groups of tympanic membranes were processed and examined histologically for differences between PGE₂ treated and control animals.

At 24 hours postperforation granulocyte infiltration at the perforation site was greater in the test animals. By 48 hours blood clots were larger in PGE₂ treated animals. Finally, the epithelium was thinner in test animals than in control animals at 96 hours.

Slight tendencies for the test animals …