Comparative and Laboratory Animal Medicine Commons^™

Two Major Ontario Hospital Labs Bought Research Dogs From U.S. Breeder With Troubled Pastdogs Bred For Scientific And Medical Testing Suffer Abuse As The Scientific Industry Increasingly Turns To Alternatives For Research., Allison Hunter, Mary Beech, Sami Marshak, Kate Morano

Capstones

The purpose-bred dog breeding industry for research is little known. Each year, thousands of dogs are bred for scientific and medical testing, supplied to research facilities by a handful of major commercial breeders. But gaps in enforcement and regulation have allowed abuses to persist, even as the industry increasingly turns to alternatives to animal testing. Animal rights advocates have been decrying the treatment of these dogs. A federal Inspector General report found lax oversight of the industry, raising further concerns about how dogs are treated. This story was reported in collaboration with the Investigative Journalism Bureau, based in Toronto, and …

Bone-Sparing Effects Of Pueraria Mirifica Extract On Bone Loss In Androgen-Deficient Cynomolgus Macaques (Macaca Fascicularis), Thaweechai Saetae, Taratorn Kemthong, Sukanya Jaroenporn, Suchinda Malaivijitnond

The Thai Journal of Pharmaceutical Sciences

Background: Synthetic estrogens provide remarkable benefits for bone health in men; however, they also present several drawbacks after long-term use. Pueraria mirifica, a phytoestrogen-rich herb, has garnered attention due to its positive effects and minimal adverse side effects on bone in females. Most research on bone health has been conducted on rodents; however, bone architecture and remodeling in rodents differ from those in humans, making it challenging to apply the results to clinical trials.
Objectives: Thus, this study assessed the bonesparing effects of P. mirifica extract (PME) in androgen-deficient male cynomolgus macaques.

Materials and Methods: Adult male macaques were divided …

Hematologic And Serum Biochemical Reference Intervals For Formosan Sika Deer (Cervus Nippon Taiouanus) From A Semi-Free-Ranging Population In Taiwan, Chen-Wei Wang, Ke-Lun Chen, Hung-Yi Wu, Chia-Ying Lin

The Thai Journal of Veterinary Medicine

The Formosan sika deer (Cervus nippon taiouanus), an endemic Taiwanese subspecies, holds a pivotal role in local conservation. This study addresses a significant knowledge gap by establishing population-specific reference intervals (RIs) for this species, as existing RIs are based on limited captive populations and may not accurately reflect the health of semi-free-ranging cohorts. We analyzed 632 sampling events from 232 healthy adult deer within Kenting National Park over 12 years (2013–2024). This study adhered to ASVCP guidelines, establishing instrument-specific RIs and employing linear mixed-effects models (LMMs) to evaluate the complex interactions among analytical instruments, sex, and body weight. …

Extended-Release Buprenorphine With Bupivacaine Effectively Provides Postoperative Analgesia In A Mouse Laparotomy Model, Monika K. Huss, Amber Kerk, Renee Cotton, Helen Chum, Michael Hsieh, Katechan Jampachaisri, Patrick Sharp, Cholowat Pacharinsak

The Thai Journal of Veterinary Medicine

Multimodal analgesia is widely encouraged for major survival surgery. This facilitates lower doses of individual drugs, thereby reducing side effects. Extended-release analgesics offer sustained analgesia coverage, prevent drug dosing nadirs, and reduce stress. Studies evaluating multimodal and extended-release analgesia combinations for major survival surgery in mice are limited. We compared the analgesic effect of bupivacaine and extended-release buprenorphine (Bupi-ER) to bupivacaine and buprenorphine HCl (Bupi-HCl). We hypothesized that preoperative Bupi-ER would provide more effective analgesia than Bupi-HCl. Mice were randomly assigned to 1 of 2 treatment groups: bupivacaine (2 mg/kg, local infiltration, once) with either 1) extended-release buprenorphine (0.6 mg/kg, …

Ahr Activation At The Air-Blood Barrier Alters Systemic Microrna Release After Inhalation Of Particulate Matter Containing Environmentally Persistent Free Radicals, Ankit Aryal, Ashlyn C. Harmon, Alexandra Noël, Qingzhao Yu, Kurt J. Varner, Tammy R. Dugas

School of Public Health Faculty Publications

Particulate matter containing environmentally persistent free radicals (EPFRs) is formed when organic pollutants are incompletely burned and adsorb to the surface of particles containing redox-active metals. Our prior studies showed that in mice, EPFR inhalation impaired vascular relaxation in a dose- and endothelium-dependent manner. We also observed that activation of the aryl hydrocarbon receptor (AhR) in the alveolar type-II (AT-II) cells that form the air-blood interface stimulates the release of systemic factors that promote endothelial dysfunction in vessels peripheral to the lung. AhR is a recognized regulator of microRNA (miRNA) biogenesis, and miRNA control diverse signaling pathways. We thus hypothesized …

Safety Study Of Polyunsaturated Fatty Acids Extracted From New Zealand Green Lipped Mussel And Krill On Hematology And Blood Chemistry Profiles Of Healthy Dogs And Cats, Uttra Jamikorn, Panumas Kongpanna

The Thai Journal of Veterinary Medicine

New Zealand green-lipped mussel (Perna canaliculus) is a shellfish with a green shell containing many types of fatty acids, especially polyunsaturated fatty acids (PUFAs). The aim of the present study was to investigate the safety of dietary PUFAs supplementation on blood hematology, chemistry and coagulogram pancreatic enzymes in dogs and cats. Twelve healthy beagle dogs and healthy mature cats were used in these studies. Complete randomized block and triplicate 4×4 Latin square designs were used for these dog and cat safety studies, respectively. Experimental diets composed of sham control (0X) and 3 treatment groups, including 1X, 3X, and …

Impact Of Voluntary Exercise On Systemic Inflammation In A 2? Hit Model Of Heart Failure With Preserved Ejection Fraction., Heather Quiriarte, Eunhan Cho, Youyoung Kim, Jake E. Doiron, Gregory Davis, James Stampley, Robert C. Noland, Zhen Li, Sanjiv J. Shah, Brian A. Irving, Guillaume Spielmann, David J. Lefer, Timothy D. Allerton

School of Medicine Faculty Publications

No abstract provided.

Leveraging Clinical Standards To Improve The Translational Relevance Of Therapies For Experimental Spinal Cord Injury, Aaron L. Silverstein

Theses and Dissertations--Neuroscience

Efforts to improve treatment of spinal cord injury (SCI) often use animals to develop effective therapeutics, intending to improve the survival and the quality of life of persons with SCI. Over half of all SCIs in the United States occur at the cervical level and often cause locomotor deficits and life-threatening breathing dysfunction. Thus, my dissertation is focused on this important patient population.

After beginning with a description of translational research and surveying SCI from a clinical standpoint, I turned to describe experimental models of cervical SCI and more detailed neuroimmunological findings relevant to my work. Then, I conducted a …