Veterinary Medicine Commons^™

Investigation Of Three Single-Nucleotide Polymorphisms (1252 G>A, 2534 G>A, And 2569 T>C) Of The Mbl1 Gene In Anatolian Buffaloes, Esma Gamze Aksel, Bi̇lal Akyüz

Turkish Journal of Veterinary & Animal Sciences

It has been reported that since the MBL gene is involved in the first step of infection and inflammation processes, it may be associated with susceptibility to bacterial and viral diseases in living organisms. In this study, it was aimed to determine the genotypic variation of Anatolian buffaloes in terms of three (1252G>A, 2534 G>A, and 2569 T>C) single nucleotide polymorphisms (SNPs) within the MBL-1 gene. For this purpose, 103 Anatolian buffaloes were genotyped for relevant SNPs by PCR-RFLP process. The allele and genotype frequencies of the examined samples were determined, and the population was examined for …

Rumen-Protected Methionine Supplementation Improves Lactation, Oxidative Status, And Immune Response During A Subclinical Mastitis Challenge In Lactating Dairy Cows., Anita Paz Lopez

Electronic Theses and Dissertations

Mastitis is a significant problem in the dairy industry, posing numerous challenges for dairy farmers worldwide. Mastitis refers to the inflammation of the mammary gland, primarily caused by bacterial infections. This condition affects the health and well-being of dairy cows and has severe economic implications for farmers and overall dairy production. The prevalence of mastitis substantially threatens milk quality, productivity, and profitability, making it a top concern in the dairy industry. Preventing mastitis in cows is crucial for maintaining the herd's health and ensuring highquality milk production. Nutritional strategies have recently gained greater attention due to a direct impact on …

Reproductive Trade-Offs In The Colorado Checkered Whiptail Lizard (Aspidoscelis Neotesselatus): An Examination Of The Relationship Between Clutch And Follicle Size, Hannah E. Caracalas, S. S. French, S. B. Hudson, B. M. Kluever, A. C. Webb, D. Eifler, A. J. Lehmicke, Lise M. Aubry

USDA Wildlife Services: Staff Publications

Life history theory predicts that there should be an inverse relationship between offspring size and number, because individuals cannot simultaneously maximize both when resources are limited. Although extensively studied in avian species, the occurrence and determinants of reproductive tradeoffs in oviparous reptiles are far less understood, particularly in parthenogenetic species. We studied this trade-off in the Colorado Checkered Whiptail, Aspidoscelis neotesselatus, a female-only parthenogenetic lizard. Using data previously collected in 2018 and 2019, we tested for clutch and egg size trade-offs and determined whether this relationship could be influenced by female size and aspects of physiological condition. Physiological condition …

Comparison Of Performance Parameters, Stress, And Immunity Levels Of Native Andcommercial Layers Reared In Different Cage Densities In Turkey, Uğur Özentürk, Ahmet Yildiz

Turkish Journal of Veterinary & Animal Sciences

In this study, production performance, stress, and immunity levels of native Turkish and imported commercial laying hens were investigated in two different cage densities. In the trial, a total of 06 groups were formed by using two different cage densities (312.50 and 468.75 cm$^{2}$/hen) on each of 03 different laying hen hybrids of Isa Brown (IB), Atak-S (A-S) and Novogen White (NW). The trial was carried out with 09 replicates in each group having 10 birds per replicate making 540 birds in total. Water and feed were provided as ad libitum. It was found that the native hybrid showed lower …

Competing Reproductive And Physiological Investments In An All‑Female Lizard, The Colorado Checkered Whiptail, Lise M. Aubry, Spencer B. Hudson, Bryan M. Kluever, Alison C. Webb, Susannah S. French

USDA Wildlife Services: Staff Publications

Organisms in the wild have to allocate limited resources towards competing functions such as reproduction, growth, and self-maintenance. These competing investments create significant changes in physiological activity, and we still know little about the relationship between physiological activity and reproductive investment in natura. We investigated trade-offs between physiological activity and reproductive investment in the parthenogenetic Colorado checkered whiptail, Aspidoscelis neotesselata, across three different sites at the US Army Fort Carson Military Installation near Colorado Springs, CO, through-out the reproductive season in 2018 and 2019. We measured clutch size and reproductive activity and quantified plasma corticosterone (CORT), reactive oxygen metabolites (ROMs), …

Comparison Of Igg And Semiquantitative Tests For Evaluation Of Passive Transfer Immunity In Calves, Onur Topal, Hasan Batmaz, Zafer Meci̇toğlu, Ender Uzabaci

Turkish Journal of Veterinary & Animal Sciences

Serum immunoglobulin (IgG) and semiquantitative tests are used for the evaluation of passive transfer immunity (PTI) in calves. We aimed to evaluate PTI in calves by using a Brix refractometer, total protein (TP), gamma-glutamyl transferase (GGT), and glutaraldehyde coagulation test (GCT) on days 1, 3, and 7 after birth; to compare the results with serum IgG; and to evaluate which day these tests are given on will give the best results. The blood samples were collected from 60 Holstein dairy calves on day 0, just after birth, and on the 1st, 3rd, and 7th days after birth. The serum IgG …

Quantitation Of Anti-Pythium Insidiosum Antibodies Before And After Immunotherapy In Healthy Dogs, Carmen Beatriz Arsuaga

LSU Master's Theses

Pythium insidiosum is an aquatic oomycete that causes invasive, progressive granulomatous lesions of the skin in dogs, horses, and cats, and of the gastrointestinal tract in dogs. Quantitation of anti-P. insidiosum IgG antibodies can be used in dogs to both confirm a suspected diagnosis and to monitor response to therapy. Recently, an immunotherapeutic product (IP) has been marketed for the treatment of pythiosis in dogs, horses, and people. The aim of this study was to evaluate the effect of administration of this product on anti-P. insidiosum IgG concentrations in dogs. The IP was administered to seven, healthy hound mixes on …

In Ovo Feeding With Ss-Hydroxy Ss-Methylbutyrate And Broilerperformance, Intestinal Health, And Immunity Status, Ömer Hakan Muğlali, Mustafa Salman, Ayhan Gacar, Fi̇li̇z Akdağ, Mustafa Yavuz Gülbahar, Alper Çi̇ftçi̇, Ertan Emek Onuk, Fi̇li̇z Karadaş, Hüseyi̇n Eseceli̇

Turkish Journal of Veterinary & Animal Sciences

The effects of in ovo administration of β-hydroxy-β-methylbutyrate (HMB) on broiler performance, intestinal health, and immunity were investigated. At day 18 of incubation, fertile eggs were divided into negative (no injection) and positive (physiologic serum injection) controls and three HMB treatment groups. HMB solution was injected at 0.1% (Group 1), 0.2% (Group 2), and 0.3% (Group 3) concentrations. There were no significant differences between the groups for live weight gain, feed conversion rate, and hot carcass, breast meat, liver, and heart weight. However, the gizzard weight in Group 1 was significantly higher than those in the other groups (P < 0.05). Although the intestinal villi lengths in the treatment Groups 1 and 3 on day 20 were greater than those in the control groups (P < 0.01), at the end of the experiment there were no significant differences among the groups. For antibody levels, the differences between groups on days 4 and 42 were highly significant (P < 0.01), and the antibody levels in Group 2 were higher than those in the other groups. Separately, growth performance and antibody levels were positively correlated with the length of villi in Group 2 (HMB 0.2%).

The Role Of Porcine Reproductive And Respiratory Syndrome Virus As A Risk Factor In The Outbreak Of Porcine Epidemic Diarrhea In Immunized Swine Herds, Mei-Zhou Huang, Hui Wang, Sheng-Yi Wang, Dong-An Cui, Xin Tuo, Yong-Ming Liu

Turkish Journal of Veterinary & Animal Sciences

The aim of the study was to investigate the relation between porcine epidemic diarrhea (PED) and porcine reproductive and respiratory syndrome virus (PRRSV). Here, we constructed a dynamic clinical experimental model in which sows were infected with PRRSV. The stability of the experimental model was surveyed by detecting the levels of antibodies against classical swine fever virus (CSFV) or PRRSV, respectively. In both group A (infected group) and group B (healthy group), the CSFV antibody level was not significantly different (P > 0.05). In group B, the PRRSV antibody level was also not significantly different (P > 0.05) at the three times …

Molecular Characterization And Expression Analysis Of Mir-29a In Porcine Cells And Porcine Reproductive And Respiratory Syndrome Virus Infected Peripheral Blood Mononuclear Cells, Win Surachetpong, Sunaree Nantakhruea, Porntippa Lekcharoensuk

The Thai Journal of Veterinary Medicine

MicroRNAs are a group of small non-coding RNA that are involved in posttranscriptional regulation through target mRNA degradation or inhibition of protein synthesis. The objective of this present study was to characterize and determine expression level of porcine miR-29a in various tissues. Our results indicated that porcine miR-29a shared high conserved sequence with miR-29a from other organisms. Furthermore, in silico analysis suggested that the seed region of porcine miR-29a partially matched IFN-γ mRNA. RT-PCR analysis indicated that miR-29a expressed in various tissues including lymph node, spleen, peripheral blood mononuclear cells (PBMCs) and PK-15 cells. Expression profiling of miR-29a in PRRSV-infected …

Analysis Of Mirna-29a Expression In Porcine Peripheral Blood Mononuclear Cells Using Quantitative Reverse Transcription Polymerase Chain Reaction, Sunaree Nantakhruea, Porntippa Lekcharoensuk, Win Surachetpong

The Thai Journal of Veterinary Medicine

MicroRNAs (miRNAs) are small non coding RNA that regulate gene expression as post transcriptional regulators in mammalian tissues. One of the miRNAs that participates in mammalian immune response is miR-29a. Despite the limited information of miR-29a in porcine cells, it is interesting to determine whether miR-29a involves in immune regulation in pig. In this study, we developed a quantitative reverse transcription polymerase chain reaction using SYBR detection system to examine the expression of primary miR-29a (pri-miR-29a) in porcine peripheral blood mononuclear cells (PBMCs) in the presence of mitogen. PBMCs were stimulated with concanavalin A (Con A) at a concentration of …

Biotin Affects The Immune Response Of Piglets Inoculated With Porcine Circovirus Type 2, Chen Hong, Zhang Keying, Ding Xuemei, Chen Daiwen

Turkish Journal of Veterinary & Animal Sciences

Weanling crossbred pigs were used in a 5-week trial to evaluate the effects of biotin and porcine circovirus type 2 (PCV2) challenge on serum cytokine concentrations and humoral immunoresponse. Although not significant, there was a trend towards reduced interferon-\gamma concentration in the challenged pigs compared to the controls, especially at 14 and 21 days postinoculation (dpi). Biotin supplementation with 50 or 200 µg/kg biotin improved these levels, with higher doses producing an earlier and stronger response. Although the magnitude of the immune response was small and inconsistent, diet supplementation with 50 or 200 µg/kg biotin appeared to increase serum immunoglobulin …

Identifying Blood Biomarkers And Physiological Processes That Distinguish Humans With Superior Performance Under Psychological Stress., Amanda M. Cooksey, Nausheen Momen, Russell Stocker, Shane C. Burgess

College of Veterinary Medicine Publications and Scholarship

BACKGROUND: Attrition of students from aviation training is a serious financial and operational concern for the U.S. Navy. Each late stage navy aviator training failure costs the taxpayer over $1,000,000 and ultimately results in decreased operational readiness of the fleet. Currently, potential aviators are selected based on the Aviation Selection Test Battery (ASTB), which is a series of multiple-choice tests that evaluate basic and aviation-related knowledge and ability. However, the ASTB does not evaluate a person's response to stress. This is important because operating sophisticated aircraft demands exceptional performance and causes high psychological stress. Some people are more resistant to …

Characterization Of The Equine 2'-5' Oligoadenylate Synthetase 1 (Oas1) And Ribonuclease L (Rnasel) Innate Immunity Genes, Jonathan J. Rios, Andrey A. Perelygin, Maureen T. Long, Teri L. Lear, Andrey A. Zharkikh, Margo A. Brinton, David L. Adelson

Veterinary Science Faculty Publications

BACKGROUND: The mammalian OAS/RNASEL pathway plays an important role in antiviral host defense. A premature stop-codon within the murine Oas1b gene results in the increased susceptibility of mice to a number of flaviviruses, including West Nile virus (WNV). Mutations in either the OAS1 or RNASEL genes may also modulate the outcome of WNV-induced disease or other viral infections in horses. Polymorphisms in the human OAS gene cluster have been previously utilized for case-control analysis of virus-induced disease in humans. No polymorphisms have yet been identified in either the equine OAS1 or RNASEL genes for use in similar case-control studies.

RESULTS: …

Comparative Analysis Of Vertebrate Eif2ak2 (Pkr) Genes And Assignment Of The Equine Gene To Eca15q24-Q25 And The Bovine Gene To Bta11q12-Q15, Andrey A. Perelygin, Teri L. Lear, Andrey A. Zharkikh, Margo A Brinton

Veterinary Science Faculty Publications

The structures of the canine, rabbit, bovine and equine EIF2AK2 genes were determined. Each of these genes has a 5' non-coding exon as well as 15 coding exons. All of the canine, bovine and equine EIF2AK2 introns have consensus donor and acceptor splice sites. In the equine EIF2AK2 gene, a unique single nucleotide polymorphism that encoded a Tyr329Cys substitution was detected. Regulatory elements predicted in the promoter region were conserved in ungulates, primates, rodents, Afrotheria (elephant) and Insectifora (shrew). Western clawed frog and fugu EIF2AK2 gene sequences were detected in the USCS Genome Browser and compared to those of other …

A Study On The Effects Of Levamisole On The Immune System Of Rainbow Trout (Oncorhynchus Mykiss, Walbaum), Ünal İspi̇r, Mustafa Dörücü

Turkish Journal of Veterinary & Animal Sciences

The effects of levamisole on the specific and non-specific immune mechanisms of rainbow trout (Oncorhynchus mykiss, Walbaum) were examined. The weight of the 60 fish used (30 in the control group and 30 in the experimental group) in this study was 99.4-216.0 g. After injecting 5 mg kg-^1 of levamisole intraperitonally, blood was taken from the heart of 30 anesthetized fish and hematocrit, leucocrit, leucocyte level, glass-adherent Nitroblue tetrazolium (NBT) positive cell activation, NBT activities, phagocytic activity, potential killing activities of neutrophyl and monocytes, myeloperoxidase production (MPO), immunoglobulin, lysozyme and ceruloplasmin (Cp) activity were determined on days 3, 7, 10 …

Equine Immunity To Cyathostome Infections, Marie Alexandra Baudena

LSU Doctoral Dissertations

To study the protective responses of cyathostome-infected ponies, two challenges were performed employing animals with different histories of exposure to these parasites. The hypothesis developed and to be tested in these experiments was that ponies that had longer exposure to cyathostome contaminated pastures would express acquired resistance to infection. The assumption behind this hypothesis was that helminth-naïve ponies infected with cyathostomes would eliminate the infection using only innate immune responses. Whereas previously exposed ponies would eliminate the infection with acquired immune responses, and these would be more effective in ponies with longer exposure to cyathostomes. Thus, helminth-naïve animals would acquire …

The Effect Of Aging On The Immune Response To Vaccination In The Horse, Daniel H. Fermaglich

LSU Master's Theses

Vaccination programs are designed to protect an animal from infection, however, depending upon the age and health of the animal vaccination may not stimulate a protective humoral response. It is possible that, as in the human and mouse models, geriatric equines may be less responsive than their younger counterparts to current vaccination protocols. The purpose of this study was to identify an age related diminution in the primary and secondary immune responses of geriatric horses in response to vaccination. Two groups of horses were sampled. The first group consisted of an open herd of 39 privately owned horses, varying in …

Evaluation Of Immunogenicity Of Pasterurella Haemolytica Serotypes In Experimental Models, K. Serdar Di̇ker, Mehmet Akan, Osman Kaya

Turkish Journal of Veterinary & Animal Sciences

The immunogenicity of Pasteurella haemolyticaserotypes was investigated in an experimental animal model. Rabbits were immunized with bacterins and leukotoxins of selected serotypes of P. haemolytica,and the IgG level and antileukotoxin activities of immune sera were measured. The highest IgG levels against A1, A2, A7 and T4 antigens were detected in rabbits receiving polyvalent combined (bacterin plus leukotoxin) immunogens. Monovalent combined immunogens induced high antibody titers against homologous serotypes, but low titers against heterologous serotypes. Serotype A1 and A7 were found to be more antigenic than A2 and T4. The highest antileukotoxin activity in monovalent groups was detected in A1 and …

The Effects Of Various Level Of Ascorbic Acid On The Immunity Andegg Yield Of Laying Hens, Behiç Coşkun, Fatma İnal, Osman Erganis, Yahya Kuyucuoğlu, Ümran Ok, İlhami Çeli̇k, Ali Muhtar Ti̇fti̇k, Firuze Kurtoğlu

Turkish Journal of Veterinary & Animal Sciences

This research was carried out in two experiments. In first experiment, three different levels of vitamin C (0, 50 and 100 mg/kg feed) were added the diets based on corn, and the effects of the diets on egg yields, blood vitamin levels, T-lymphocyte percentage, plasma cell count in spleen and antibody titers against the vaccine of Newcastle disease were investigated for one year. Total of 648 laying hens were used in this experiment. The group fed the diet added 50 mg/kg vitamin C, produced less egg and fed more feed for one kg egg than other groups. There were no …