Veterinary Medicine Commons^™

Detection Of Leptospira Species By Polymerase Chain Reaction (Pcr) In Urine Of Cattle, Burhan Çeti̇nkaya, Hasan Basri̇ Ertaş, Hasan Öngör, Adi̇le Muz

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to investigate the prevalance of leptospirosis in the urine of cattle slaughtered in three major abattoirs in the east of Turkey. A polymerase chain reaction (PCR) based on a pair of genus-specific primers was used to detect leptospiral DNA in the urine samples of 473 cattle, 284 of which were from Elazig, 112 from Malatya and 77 from Diyarbakir. The detection limit of the method was determined to be approximately five bacteria per ml of urine. In the examination of urine samples, 4.02% (19/473) (95% confidence intervals [Cl] 2.4-6.2) were found to be positive by …

Determination Of Prevalence Of Paratuberculosis In Dairy Cattle By Polymerase Chain Reaction (Pcr), Burhan Çeti̇nkaya, Adi̇le Muz, Hasan Basri̇ Ertaş, Hasan Öngör, İ. Yavuz Sezen, H. Basri̇ Gülcü

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the prevalence of paratuberculosis in dairy cattle in ElazİÛ and the surrounding villages. A polymerase chain reaction (PCR) based on IS900, an insertion sequence specific to Mycobacterium avium subsp. paratuberculosis, was used to detect mycobacterial DNA in milk samples of 500 dairy cows. The detection limit of the PCR was assessed by spiking a known negative milk sample with a dilution series (1x10 9 to 10 bacteria/ml) of reference M. avium subsp. paratuberculosis culture). In the agarose gel examination of the PCR amplified products, approximately 50 bacteria/ml were detected. In the examination …

A Survey Of Hypodermosisin Cattle Slaughtered In Thrace (Trakya) Turkey, Aynur Gülanber, Erkut Tüzer, Ayşen Gargili, Müfi̇t Toparlak, İlker Efi̇l, Vedat Keleş, Meltem Ulutaş

Turkish Journal of Veterinary & Animal Sciences

In this study, a total of 365 cattle (234 male, 131 female) slaughtered in different localities in Thrace (The European part of Turkey) were examined for hypodermosis between January and May of 1997. Hypoderma bovis, H. lineatum and unidentified 2nd instar larvae were found in the skin and subcutaneous tissue of the back region of the animals. The prevalence rate and intensity of infestation were 3.56% and 10.23 respectively.

The Effects Of Weaning Age On The Growth, Milk And Milk Fat Characteristics Of Brown Swiss Cattle, Mete Yanar, Recep Aydin

Turkish Journal of Veterinary & Animal Sciences

In this research, the effects of early weaning on the growth rate and milk production characteristics of Brown Swiss cattle in later years were investigated. The calves used in the study were weaned at 5, 7 and 9 weeks of age, and the weights obtained at 6, 9, 12, 15 and 18 months of age were not significantly affected by the age of weaning. The weight gains determined between 6 and 18 months of ages with 3-month intervals were also not significantly affected by the different ages of weaning. The various ages of weaning did not result in a significant …

Determination Of Anti- Toxoplasma Gondiiantibodies In Cattle In Elazığ And Environs By Sabin-Feldman (Sf) Test, Müni̇r Aktaş, Cahi̇t Babür, K. Zafer Karaer, Nazi̇r Dumanli

Turkish Journal of Veterinary & Animal Sciences

Toxoplasma gondii pseudocysts in cattle are sources of infection for humans. Therefore, cattle toxoplasmosis is a very important factor in public health. The aim of the present study was to determine the seroprevalence of cattle toxoplasmosis. A total of 122 cattle from different age groups were selected in Elazığ and environs. Blood samples were taken and sera were collected. Serum samples were kept at -20^0 C until further examination. The values of toxoplasmosis seroprevalence were determined by SF test for anti-Toxoplasma gondii antibodies. Antibodies were detected in 86 out of 122 cattle sera (70.49%). Fifty-four (62.75%), 26 (30.23%), 6 (6.97%) …

Efficacy Of Moxidectin Against Gastrointestinal Nematodesin Naturally Infected Lambs And Calves, Erkut Tüzer, Müfi̇t Toparlak, Kazim Göksu

Turkish Journal of Veterinary & Animal Sciences

In this trial based on the reduction of the egg output and on the evaluation of results from larval/egg differentiation, moxidectin (Cydectin, 1% injectable moxidectin) administrated subcutaneously at a dose of 0.2 mg kgbw -1 was found effective on the gastrointestinal nematodes in naturally infected lambs (8 female and 7 male treatment; 8 female and 7 male control) harbored Haemonchus spp, Ostertagia spp, Trichostrongylus spp and Nematodirus spp and in naturally infected female calves (20 treatment and 10 control) harbored Haemonchus spp, Ostertagia spp, Trichostrongylus spp, Bunostomum sppand Cooperia spp.The efficacy was 100% on day +7, 99.9% on day +14 …

Prevalence Of Liver Fluke Infections In Slaughtered Animals In Trakya(Thrace), Turkey, Ayşen Gargili, Erkut Tüzer, Aynur Gülenber, Müfi̇t Toparlak, İlker Efi̇l, Vedat Keleş, Meltem Ulutaş

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to determine the prevalence of liver fluke infections in cattle and sheep in Trakya (The European Part of Turkey). For this purpose the slaughterhouses of different localities (Tekirdağ, Hadimköy, Lüleburgaz, Çorlu, Kırklareli and Keşan) were visited between 03.03.1997 and 22.10.1997, and a total of 476 sheep and 415 cattle livers were examined. The prevalence rates of fasciolosis hepatica and dicrocoeliosis dendriticum were 3.99% and 23.55% in sheep, and 0.48% and 2.65% in cattle respectively. No changes in livers, which led to the partial or total condemnation, were observed.

Effect Of Macroscopic Classification Of Bovine Ovarian Follicles On Steroidogenicactivities Of Granulosa Cells In Vitro, Mehmet Kuran

Turkish Journal of Veterinary & Animal Sciences

This study aimed to investigate relationships between steroidogenic characteristics and abilities, and some macroscopic characteristics of bovine ovarian follicles and to relate this criterion to the physiological status of the follicles. Follicular fluid of 5 to 8 mm diameter follicles from bovine ovaries (n=46) were analysed and steroidogenic activities of granulosa cells were studied. For the macroscopic classification of the follicles (n=232), appearence of the follicles, thecal vascularization, the colour of theca interna and the presence of follicular debri were determined. Follicles were classified as non-atretic, intermediate and atretic using the cri-terion above. Oestradiol-17b concenration was higher than progesterone concentration …

Heritability Estimation Of Milk Production Of Cows Of Different Origin And Breed In Karacabey State Farm, İsmet Doğan, Okan Ertuğrul

Turkish Journal of Veterinary & Animal Sciences

The objective of this study was to estimate heritability (h^2), a useful criterion, in the improvement studies which would be conducted to improve milk productivity in the Karacabey State Farm. A total of 760 cows, of which milk yield records were used in the study in different lactation period, consisted of 111 American Browns , 142 American Holsteins , 87 German Browns , 130 Swiss Browns , 83 Holland Holsteins and 207 Karacabey Browns. Milk yields were corrected according to the 2x305ME standardisation procedure. Furthermore, coefficients of relationship were calculated separately for Brown and Holstein cows using the pedigree cards …

Determination Of Seroprevalence Of Leptospirosis In Cattle In Elazığ, Burhan Çeti̇nkaya, Hasan Basri̇ Ertaş, Adi̇le Muz, Hasan Öngör, Hakan Kalender, Vi̇ldan Özdemi̇r

Turkish Journal of Veterinary & Animal Sciences

Serum samples collected from randomly-selected 395 cattle between June-November 1997 from the districts and central villages of Elazığ were used to determine the seroprevalence of Leptospirosis in cattle. Microscopic Agglutination Test (MAT) was used to examine serum samples for antibodies to L. hardjo, L. grippotyphosa, L. pomona and L. icterohaemorrhagiae. Positive results were obtained from eight (2.03%) samples; seven (1.77%) of which were agglutinated with L. hardjo and one (0.25%) with L. grippotyphosa antigens. There was a statistically significant difference when the seropositivity rates were compared in different districts (p=0.002). Age and sex were not determined to affect the frequency …

A Field Evaluation Of Ivomec-F For The Treatment Of Naturally Acquired Fasciola Gigantica And Other Gastrointestinal Nematode Infections In Cattle And Buffaloes, Tasanee Chompoochan, Suwannee Nithiuthai, Piyanoot Prasittirat

The Thai Journal of Veterinary Medicine

The efficacy of Ivomec-F was evaluated in 60 cattle and 14 buffaloes that had a naturally acquired infection of Fasciola gigantica. Some animals were also concurrently infected with gastrointestinal nematodes. The animals were divided equally into treatment and control groups. Treatment group 1 which consisted of 30 cattle, were administered a single subcutaneous dose of Ivomec-F (1% ivermectin /10% clorsulon) 1 ml/ 50 kg body weight. Control group 2 which consisted of 30 untreated cattle, were given the same volume of physiological saline. Groups 3 and 4 (7 treated and 7 control buffaloes) were treated in a similar manner as …

Development Of A Blocking Enzyme-Linked Immunosorbent Assay For Detection Of Serum Antibodies To O157 Antigen Of Escherichia Coli., W Laegreid, M Hoffman, J Keen, R Elder, J Kwang

Manuscripts, Articles, Book Chapters and Other Papers

The O157 antigen of Escherichia coli shares structural elements with lipopolysaccharide (LPS) antigens of other bacterial species, notably Brucella abortus and Yersinia enterocolitica 09, a fact that confounds the interpretation of assays for anti-O157 antibodies. To address this problem, a blocking enzyme-linked immunosorbent assay (bELISA) was designed with E. coli O157:H7 LPS as the antigen and a monoclonal antibody specific for E. coli O157, designated 13B3, as the competing antibody. The bELISA had equivalent sensitivity to, and significantly higher specificity than, the indirect ELISA (iELISA), detecting anti-O157 antibodies in sera from cattle experimentally inoculated with O157:H7. Only 13% of sera …

Eosinophilic Myositis In A Cattle, Ayhan Atasever

Turkish Journal of Veterinary & Animal Sciences

Eosinophilic myositis was described in the M. longissimus lumborum, M. latissimus dorsi, M. gluteus muscles of Holstein bull aged 18 month-old. Diffuse lesions were seen grossly. They were well-demarked, in green colour and 2-3 mm in diameter. Diffuse eosinophilic myositis were diagnosed microscopically.

Prevalence Of Bovine Eimeridosis In Thracia, Turkey, M. Özkan Arslan, Erkut Tüzer

Turkish Journal of Veterinary & Animal Sciences

In this study, 768 faecal samples were examined between June 1993-May 1994 to determine the species and the prevalence rates of Eimeriidae in the cattle of Thracia area (The European part of Turkey). The prevalence rate of eimeridosis was found to be 68%. The species determined and their prevalence rates were as follows: Eimeria bovis(34%), E.auburnensis(27%), E.zeuernii(26%), E.ellipsoiDalıs (14%), E.canadensis (12%), E.cylindrica (7.9%), E.supspherica (7.2%), E.alabamensis (4.9%), E.bukidnonensis (2.2%), E.brasiliensis (0.8%) and Isospora sp. (1.2%). Of 768 animals, 29% were found to be infected with single species, 39% with mixed ones and 32% were non-infected. During the study, two calves …

Prevalence Of Mix Infectionsof Crytosporidium Spp. Eschricia Coli K99 And Rotavirus In The Faeces Of Diarrhoeic And Healty Cattle In Ankara..., Zişan Emre, Hatice Fi̇danci

Turkish Journal of Veterinary & Animal Sciences

Faecai specimens from 172 diarrhoeic and 130 healthy cattle were examined for the presence of Crytosporidium spp., Eschricia coli K99 and Rotavirus in Ankara, Turkey. The prevalence of the Crytosporidium spp. infection in diarrhoeic and non-dierrhoeic cattle was 63.3% and 69.2%, respectively. Crytosporidium positive faecal specimens were examined for the presence of E. coli K99 and Rotavirus. E. coli K99 was isolated from the feaces of 35 diarrhoeic (32.1%) and 23 healthy (25.5%) animals. Rotavirus was not detected in any of feacal samples. In vitro drug sensitivities indicated that E. coli K99 was sensitive to Nalidixic acid in both diarrhoeic …

Prevalence Of Cryptosporidium Spp. Infection And Its Relation To Other Entericpathogens (Escherichia Coli K 99 And Rotavirus) In Cattle In Ankara, Turkey, Zisan Emre, B. Metin Alabay, Hatice Fi̇danci, Ali Düzgün, Harun Çerçi̇

Turkish Journal of Veterinary & Animal Sciences

Faecal samples of 172 diarrhoeic and 130 non-diarrhoeic cattle were examined for the presence of Cryptosporidium oocysts. The prevalence of infection in diarrhoeic and non-diarrhoeic cattle was 63.3% and 69.2%, respectively. The infection was prevalent in cattle over three years old, in both diarrhoeic and non-diarrhoeic animals. In both groups, oocyst sheeding level of (+) was the most common. Cryptosporidium-positive faecal specimens were examined for the presence of Escherichia coli K 99 and rotavirus. E. coli K 99 was isolated from the faeces of 35 (32.1%) diarrhoeic and 23 (25.5%) non-diarrhoeic animals. Rotavirus was not detected in any of the …

Studies On Schistosoma Spindale In Cattle And Buffalo In The Northeast Of Thailand 4. Mucosal Scraping Of Rectum For Diagnosis Of Cattle Schistosomosis (การศึกษาพยาธิใบไม้เลือด ชิสโตโซมา สปินดาเล ในโคและกระบือในภาคตะวันออกเฉียงเหนือ 4. การชันสูตรโรคพยาธิใบไม้เลือดโคโดยวิธีขูดเยื่อบุเรคตั้ม), Manvika Pholpark, Niyomsak Upatomm, Somchai Srihakim

The Thai Journal of Veterinary Medicine

Finding of Schistosoma spindale egg in cattle can be easily performed by mucosal scraping of rectum. Mucosal samples of 2-3 cm. long were scraped from dorsal side of the rectum by extended curette, spread on slide and stained with 0.015% malachite green for 10-15 minutes subsequently examined under the microscope. This method gave more accuracy results than the other faecal examination methods: saline sedimentation, formalin preserved faeces sedimentation, Kato's thick smear and MIFC techniques.(การตรวจหาไข่พยาธิใบไม้เลือดของโคสามารถทำได้ง่าย โดยการขูดเยื่อบุผนังลำไส้ด้านบนของเรคตั้ม ยาวประมาณ 3-4 ซม. ด้วยคิวเรทต่อด้าม ย้อมด้วยสี มาลาไคท์กรีน 0.015% บนสไลด์นานประมาณ 10-15 นาที แล้วนำมาตรวจด้วยกล้องจุลทรรศน์เปรียบเทียบกับผลการพิสูจน์โดยวิธีตกตะกอนด้วยน้ำเกลือเข้มข้น 1% หรือวิธีตกตะกอนธรรมดาโดยใช้อุจจาระดองฟอร์มาลิน 10% หรือเทคนิคของ Kato's thick smear และ MIFC พบว่าการตรวจด้วยวิธีขูดเยื่อบุเรคตั้มได้ผลดีกว่าวิธีอื่น ๆ)

Lupinosis : A Disease Still With Us, Jeremy G. Allen, P. Mcr. Wood, K. P. Crocker, J. Hamblin

Journal of the Department of Agriculture, Western Australia, Series 4

Current research on lupinosis continues to reveal what a complex problem it is, but the improved understanding should help to reduce losses.

Cancer Eye Of Cattle, M R. Gardiner, J. L. Anderson, D. E. Robertson

Journal of the Department of Agriculture, Western Australia, Series 4

Cancer of the eye and eyelids is mainly a disease of cattle, although it occasionally occurs in most species of animals and in man. It is the most common cancer in cattle, especially in certain breeds.

Prevent Worms In Cattle, G C De Chaneet

Journal of the Department of Agriculture, Western Australia, Series 4

PARASITIC gastro enteritis, caused by roundworms, is a common cause of economic loss in calves, yearlings and occasionally cows in south-western Australia. Losses could be prevented if stock owners realised the full effects of these parasites, and understood the causes of worm infestation.

Black-Leg In Cattle, F C. Wilkinson

Journal of the Department of Agriculture, Western Australia, Series 4

BLACK-LEG is still a serious disease of cattle in the South West coastal region, causing deaths in young cattle.

The disease is characterised by hot swollen muscles, acute lameness and rapid death.

Cattle affected are usually 6 to 12 months old, in fat condition, and grazing on lush pastures.

Eye Cancer In Cattle, E P. Meyer

Journal of the Department of Agriculture, Western Australia, Series 4

THE CONDITION known as eye cancer occurs in all ages and breeds of cattle but is most common in certain strains of the Hereford breed that have non-pigmented or mottled eyes and eyelids.

A yearly incidence of up to 5 per cent can occur in susceptible herds.

Bovine Tuberculosis Control In Western Australia : Past, Present And Future, M R. Gardiner

Journal of the Department of Agriculture, Western Australia, Series 4

TWENTY YEARS AGO, tuberculosis was a widespread and serious disease in the dairy cattle of Western Australia.

Besides having a marked depressing effect on the economy of the dairying industry, bovine tuberculosis was a constant hazard to humans coming in contact with it.

Cow's Hoofs Need To Be Watched, Department Of Agriculture, Western Australia

Journal of the Department of Agriculture, Western Australia, Series 4

THE need for thorough trimming of the hoofs of sheep for the control of foot rot is recognized, but few realize the need for trimming the hoofs of cattle.

Remarkable Success Of Bovine T.B. Eradication Campaign, J Shilkin

Journal of the Department of Agriculture, Western Australia, Series 4

Less than 20 years ago Perth dairy herds had the highest T.B. incidence of any Australian capital city and half of the Kalgoorlie cattle had the disease.

Why this was so and how the eradication campaign has reduced the incidence of less than 0.3 per cent, in 128,000 cattle is discussed in this article.

Internal Parasites Of Cattle, J Craig, R. Butler, M. E. Nairn

Journal of the Department of Agriculture, Western Australia, Series 4

Worm parasites can have a most serious effect on cattle, even causing deaths.

This article describes the main parasites of cattle, treatment with modern anthelmintic drugs and the best means of preventing and controlling worm outbreaks.

Lice On Cattle, Department Of Agriculture, Western Australia

Journal of the Department of Agriculture, Western Australia, Series 4

Lice can cause serious financial losses through reduced growth rates and loss of production in beef and dairy cattle.

This article describes the main species and recommended control measures.

Bracken Fern Poisoning In Cattle, P B. Lewis

Journal of the Department of Agriculture, Western Australia, Series 4

ONE of the major problems facing farmers in the South-West of Western Australia is the hazard of bracken fern poisoning.

Water For Agriculture. 5. Salt Poisoning In Livestock, J Craig

Journal of the Department of Agriculture, Western Australia, Series 4

LIVESTOCK need a plentiful supply of good, fresh drinking water for normal health and high production.

Over large areas of Western Australia the water from bores, springs and wells contains varying but appreciable amounts of salt and special care may be needed to ensure that the stock water supply is satisfactory.

The Dehorning Of Cattle, J Shilkin

Journal of the Department of Agriculture, Western Australia, Series 4

THE dehorning of cattle, both dairy and beef, has many advantages.

Breeders of stud cattle frequently claim that removal of the horns spoils the appearance of show animals and stress that the practice is cruel.

Whilst both these statements may, to some extent, be true, the advantages of the practice far outweigh any disadvantages.