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Full-Text Articles in Veterinary Medicine

Detection Of Bovine Tuberculosis By Tuberculin Test And Polymerase Chain Reaction In Van, Turkey, Hasan Solmaz, Zi̇ya İlhan, Abdulbaki̇ Aksakal, Ti̇mur Gülhan, İsmai̇l Hakki Eki̇n Jan 2009

Detection Of Bovine Tuberculosis By Tuberculin Test And Polymerase Chain Reaction In Van, Turkey, Hasan Solmaz, Zi̇ya İlhan, Abdulbaki̇ Aksakal, Ti̇mur Gülhan, İsmai̇l Hakki Eki̇n

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to identify the presence and prevalence of bovine tuberculosis in the eastern Anatolian border province of Van and its villages and to collect the preliminary data required for programs for the prevention and control of this zoonotic disease. The tuberculin test was performed on 210 cattle. Nasal swab and milk samples were obtained from the animals tested. Three cattle that had a positive tuberculin test also tested positive for the DNA target, a 580-bp fragment of IS6110 specific for members of the Mycobacterium tuberculosis complex. This fragment was also recovered from milk taken from …

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Seroprevalence Of Brucellosis In Human, Cattle, And Buffalo Populations In Pakistan, Iftikhar Hussain, Muhammad Imran Arshad, Muhammad Shahid Mahmood, Masood Akhtar Jan 2008

Seroprevalence Of Brucellosis In Human, Cattle, And Buffalo Populations In Pakistan, Iftikhar Hussain, Muhammad Imran Arshad, Muhammad Shahid Mahmood, Masood Akhtar

Turkish Journal of Veterinary & Animal Sciences

A total of 1500 serum samples were collected from cattle, buffaloes, and humans. The Rose Bengal plate test (RBPT) and enzyme-linked immunosorbent assay (ELISA) detected Brucella antibodies in 56 (10.18%) and 44 (8%) of the cattle, respectively. In samples collected from buffaloes 9.38% and 6.92% were positive, whereas 14% and 11% of the samples from humans were found to be positive by RBPT and ELISA, respectively. Among village workers a higher prevalence of brucellosis was recorded in females than in males. Abattoir-associated personnel also had a higher incidence of brucellosis. Results of the present study revealed that RBPT and ELISA …

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The Influence Of Epidermal Growth Factor On Maturation And Fertilisation Of Bovine Oocytes In Vitro, Bülent Polat, M. Rifat Salmanoğlu Jan 2008

The Influence Of Epidermal Growth Factor On Maturation And Fertilisation Of Bovine Oocytes In Vitro, Bülent Polat, M. Rifat Salmanoğlu

Turkish Journal of Veterinary & Animal Sciences

The aim of the present study was to evaluate the efficiency of different doses of epidermal growth factor (EGF) on maturation and fertilisation of bovine oocytes in vitro. A total of 1209 high quality oocytes (follicles 2-8 mm in diameter, with unexpanded cumulus cells) were obtained by aspiration from ovaries collected at a slaughterhouse. The oocytes were then assigned into 6 groups: 1, 10, 100, and 1000 ng/ml EGF groups, as well as positive (EGF replaced with hCG) and negative (TCM 199 only, no supplement) control groups. Maturation and fertilisation rates were calculated at 39 °C in an atmosphere of …

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Plasmids And Protein Patterns Of Escherichia Coli Isolated From Bovine Mastitis In Konya, Turkey, Uçkun Sai̇t Uçan, Leyla Açik, Ayten Çelebi̇, Osman Ergani̇ş, Emi̇ne Arslan Jan 2005

Plasmids And Protein Patterns Of Escherichia Coli Isolated From Bovine Mastitis In Konya, Turkey, Uçkun Sai̇t Uçan, Leyla Açik, Ayten Çelebi̇, Osman Ergani̇ş, Emi̇ne Arslan

Turkish Journal of Veterinary & Animal Sciences

In this study, a total of 30 Escherichia coli isolates obtained from milk samples of dairy cows suffering from subclinical mastitis in Konya, Turkey were typed according to plasmids and protein patterns. Agarose gel electrophoresis and sodium doedecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) methods were used to identify plasmids and whole-cell protein profiles. Of these two methods, SDS-PAGE typing proved to be more discriminate for typing the isolates.

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Meningitis Associated With Brucella Abortus In An Aborted Bovine Foetus, Cengi̇z Çeti̇n, İ. Taci̇ Cangül, Mehmet Akan, Ebru Özel, Ahmet Akkoç Jan 2004

Meningitis Associated With Brucella Abortus In An Aborted Bovine Foetus, Cengi̇z Çeti̇n, İ. Taci̇ Cangül, Mehmet Akan, Ebru Özel, Ahmet Akkoç

Turkish Journal of Veterinary & Animal Sciences

In this study, the brain and other organs from an aborted bovine foetus were examined histopathologically and microbiologically. In addition, a serum sample from the aborting cow was examined serologically. A granulomatous inflammation, characterised by the infiltration of lymphocytes, plasma cells with some neutrophils and macrophages was observed in the meninges of the brain, liver and kidney. Brucella abortus biovar 1 was isolated in pure culture from the brain and other organs. The serum sample was positive for brucellosis. These findings indicate that Brucella abortus is a pathogen capable of inducing meningitis in bovine foetuses.

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First Identification Of Neospora Caninum In Thailand, Than Kyaw, Prachin Virakul, Manop Muangyai, Wijit Banlunara Dec 2003

First Identification Of Neospora Caninum In Thailand, Than Kyaw, Prachin Virakul, Manop Muangyai, Wijit Banlunara

The Thai Journal of Veterinary Medicine

An immunohistochemical examination of aborted bovine tissues, collected from two dairy farms that had a high abortion rate (7%), detected the organism Neospora caninum (NC). Five out of 12 aborting cows (41.7%) were seropositive to NC using a competitive ELISA test. Neospora-tachyzoites were detected in the placenta of a seropositive aborting cow. Neither NC parasites nor cysts were detected in other fetal tissues. The identification of the Neospora-parasite and a high percentage of Neospora seropositive aborting cows, suggested NC as the possible cause of abortion. This is the first identification of the NC parasite in Thailand.

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The Presence, Isolation And Characterisation Of Galectin-1 In Bovine Adrenal Gland, Kami̇l Seyrek Jan 2003

The Presence, Isolation And Characterisation Of Galectin-1 In Bovine Adrenal Gland, Kami̇l Seyrek

Turkish Journal of Veterinary & Animal Sciences

The mammalian adrenal gland in the developmental stage provides excellent system for studying the role(s) of lectins in the foetal period because the adrenal gland is composed of two separate endocrine organs which differ in embryologic origin, type of secretion, and function. There is no information on the presence of galectin-1 or galectin-1 reactive glycoconjugates in the development of the bovine adrenal gland. Therefore, the present study focused on this protein. For separation of galectin-1 dialysis, affinity chromatography and SDS-PAGE were used. To monitor the localisation of galectin-1 and its epitopes immunhistochemistry was used. A single 14-kD band comigrated with …

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A Comparison Of The Effect Of Methyl-B-Cyclodextrin On The Osmotic Fragility Of Ovine, Bovine And Human Erythrocytes, Şevket Arikan Jan 2003

A Comparison Of The Effect Of Methyl-B-Cyclodextrin On The Osmotic Fragility Of Ovine, Bovine And Human Erythrocytes, Şevket Arikan

Turkish Journal of Veterinary & Animal Sciences

Cyclodextrins are used extensively in food, cosmetic, drug and chemical industries to increase aqueous solubility and stability, and reduce or eliminate the unpleasant taste and smell of many products. Experiments were conducted to examine the effect of a pre-haemolytic dose of methyl b-cyclodextrin (MBCD) on the osmotic fragility of erythrocytes collected from sheep, cattle and humans. Heparinised blood collected from these three species were analysed within 3 h of collection. Erythrocyte suspensions were mixed with MBCD to give concentrations of 2 mM, 4 mM or 6 mM cyclodextrin. The mixtures were incubated for 30 min at 37 °C and centrifuged. …

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Immunoperoxidase Studies On Leptospirosis In Bovine Abortion, Ahmet Temur, Yavuz Seli̇m Sağlam Jan 2003

Immunoperoxidase Studies On Leptospirosis In Bovine Abortion, Ahmet Temur, Yavuz Seli̇m Sağlam

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to determine leptospirosis from aborted bovine fetuses. Tissue sections of the liver, lung, spleen and kidney were stained with avidin-biotin peroxidase complex, and antigen localization was examined. These examinations showed positive staining in eight (24.24%) out of 33 fetuses for the presence of leptospira antigens. In these eight cases, leptospira antigens were found in three lung (37.5%), two liver (25%), four kidney (50%) and four spleen (50%) samples. Microscopic studies demonstrated that leptospiral antigens were located in the interalveolar and interlobular septum of the lung; in the cytoplasm of hepatocytes and macrophages in the portal regions …

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Transgenic Bovine Nuclear Transfer Embryos From Adult Somatic Cell Lines*, Sezen Arat, S. Jacek Rzucidlo, Steven L. Stice Jan 2003

Transgenic Bovine Nuclear Transfer Embryos From Adult Somatic Cell Lines*, Sezen Arat, S. Jacek Rzucidlo, Steven L. Stice

Turkish Journal of Veterinary & Animal Sciences

This study was performed to examine whether adult fibroblast cells can be manipulated genetically and whether clonal lines derived from those cells can support embryonic development. A primary adult cell line was established from the ear of an aged cow. Adult cells with a plasmid containing the enhancer green fluorescence protein (EGFP) gene, and non-transfected cells were used for cloning. Green fluorescence expression was observed in 35/49 (71.4%) adult clones. The developmental rates of embryos were significantly lower for cell lines expressing EGFP (11.4%) than for non-transfected cells (20.1%, P < 0.05). However, there was no decrease in nuclear transfer (NT) developmental rates (21.5%) when donor nuclei from EGFP transfected cell lines not expressing EGFP but retaining neomycin-resistance gene expression were used as donor nuclei. The NT embryos from transfected and non-transfected cell lines had similar morphology and cell numbers. The results indicated that adult cells can complete clonal propagation including transfection and selection and can be used to produce transgenic NT embryos.

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Serological And Haematological Investigations On Bovine Leukaemia Virus Infections In Kars District, Sali̇h Otlu, Fuat Aydin, Oktay Genç, M. Ali̇ Güler, Gürbüz Gökçe Jan 2001

Serological And Haematological Investigations On Bovine Leukaemia Virus Infections In Kars District, Sali̇h Otlu, Fuat Aydin, Oktay Genç, M. Ali̇ Güler, Gürbüz Gökçe

Turkish Journal of Veterinary & Animal Sciences

This study was carried out in order to serologically and haematologically determine the prevalance of Bovine Leukaemia Virus infection in cattle raised in Kars and environs.For this purpose a total of 443 blood samples and 383 milk samples belonging to the various breeds (Zavot, Holstein, Brown-Swiss and Native), and sexes (383 female, 60 male)were examined serologically (Agar Gel Immunodiffusion (AGID)and Enzyme Linked Immunosorbent Assay (ELISA))and haematologically (lymphocyt counts, total leucocyt counts and haematocrit value). All of the 443 blood samples and 383 milk samples examined serologically by AGID and ELISA tests were found to be negative in EBL.As a result …

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Changes In The Size Distribution Of Steroidogenic And Non-Steroidogenic Bovine Luteal Cells During The Oestrus Cycle, Arzu Yi̇ği̇t, Şevket Arikan Jan 2001

Changes In The Size Distribution Of Steroidogenic And Non-Steroidogenic Bovine Luteal Cells During The Oestrus Cycle, Arzu Yi̇ği̇t, Şevket Arikan

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to investigate the size distribution of steroidogenic and non-steroidogenic luteal cells throughout the oestrus cycle. Twelve corpora lutea collected from different stages of the oestrus cycle were used. Corpora lutea collected from nonpregnant cows were dissociated into single-cell suspensions by enzyme treatments. Cells were stained for 3\beta-hydroxysteroid dehydrogenase (3\beta-HSD) activity, a marker for steroidogenic cells. The sizes of 3b-HSD positive (steroidogenic) and negative cells (nonsteroidogenic) were measured with an ocular micrometer. Very small cells (

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Effects Of Different Maturation Periods On In Vitro Maturation Of Bovine Oocytes, Sema Bi̇rler, Serhat Pabuççuoğlu, İ. Kamuran İleri̇, Serhat Alkan, Mithat Evecen Jan 1998

Effects Of Different Maturation Periods On In Vitro Maturation Of Bovine Oocytes, Sema Bi̇rler, Serhat Pabuççuoğlu, İ. Kamuran İleri̇, Serhat Alkan, Mithat Evecen

Turkish Journal of Veterinary & Animal Sciences

Primer oocytes (n=157) collected from ovaries of slaughtered cows were matured at 39°C with 95-100% humidity and under a gas mixture of 5% O 2 , 5% CO 2 and 90% N 2 for 22 (n=52), 24 (n=52) and 26 (n=53) hours. Modified Parker's Medium (MPM) supplemented with FSH and 20% ECS was used as maturation medium. At the end of these periods all oocytes were fixated in ethanol:acetic acid in the ratio of 3:1 for 24 hours and stained by %2 aceto-orcein to evaluate the maturational criteria. At the 22, 24 and 26-hours maturation groups, 23(%44.2), 27 (%51.9) and …

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The Presence Of Sialic Acid In Bovine Serum Amyloid P Component (กรดไซอะลิคในซีรั่มโปรตีน Amyloid P Component ในโค), Meena Sarikaputi, Masaharu Naiki Jun 1995

The Presence Of Sialic Acid In Bovine Serum Amyloid P Component (กรดไซอะลิคในซีรั่มโปรตีน Amyloid P Component ในโค), Meena Sarikaputi, Masaharu Naiki

The Thai Journal of Veterinary Medicine

Neuraminidase-treated bovine serum amyloid P component (SAP) has chromatographic profile from DEAE-HPLC column of shorter retention time and less peak number than intact molecule does. SDS-PAGE analysis reveals a decrease in size of bovine SAP subunits after neuraminidase treatment, compared with an untreated molecule. This study indicates a presence of sialic acid in bovine SAP.(จากการวิเคราะห์ซีรั่มโปรตีน amyloid P component (SAP) ในโคด้วยเครื่อง High performance liquid chromatography (HPLC) โดยใช้คอลัมภ์ DEAE พบว่า ซีรั่มโปรตีน SAP ที่ผ่านการย่อยด้วยเอนไซม์ neuraminidase มี retention time สั้นกว่า และจำนวน peak ของโปรตีนน้อยกว่าซีรั่มโปรตีนชนิดเดียวกันที่ไม่ได้ผ่านการย่อย จากการ วิเคราะห์ด้วยอิเลคโตรฟอเรซีสบนเจล SDS-PAFE พบว่าหน่วยย่อยของซีรั่มโปรตีน SAP ที่ผ่านการย่อยด้วยเอนไซม์ neutraminidase มีขนาดเล็กลงเมื่อเทียบกับซีรั่มโปรตีนชนิดเดียว กันในสภาพธรรมชาติ ผลการวิเคราะห์โดยวิธีดังกล่าวแสดงให้เห็นว่ามีกรดไซอะลิคเป็น ส่วนประกอบอยู่ในโมเลกุลของซีรั่มโปรตีน SAP ในโค)

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Environmental Effects On The Quality Of Bovine Semen(ผลของสภาวะแวดล้อมต่อคุณภาพของน้ำเชื้อโคและกระบือ), M. Subhan Quereshi, H.A. Samad, Zabita Khan, Shafgat Hussain Mar 1995

Environmental Effects On The Quality Of Bovine Semen(ผลของสภาวะแวดล้อมต่อคุณภาพของน้ำเชื้อโคและกระบือ), M. Subhan Quereshi, H.A. Samad, Zabita Khan, Shafgat Hussain

The Thai Journal of Veterinary Medicine

The interaction between semen quality of Holstein Friesian, Jersey, Crossbred and Nili-Ravi buffaloes and the environment was studied. Semen was collected for one year at the Semen Production Unit, Surizai District, Peshawar from 10 Holstein Friesian, 4 Jersey and 4 Crossbred bulls and 3 buffaloes. Semen was collected early in the morning, twice weekly, using an artificial vagina. Environmental data regarding the monthly average temperature, humidity and daylight, was recorded. The correlation co-effi-cient between the semen volume, motility and sperm concentration and the three environmental parameters was calculated. Statistical analysis showed that in buffalo bulls motility was significantly (r> 0.5874) …

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Complete Sequence Of Bovine C-Reactive Protein Complementary Dna, Meena Sarikaputi, Masami Morimatsu, Masayki Saito, Masaharu Naiki Dec 1993

Complete Sequence Of Bovine C-Reactive Protein Complementary Dna, Meena Sarikaputi, Masami Morimatsu, Masayki Saito, Masaharu Naiki

The Thai Journal of Veterinary Medicine

Bovine CRP cDNA of 2.2 kb was isolated from bovine liver cDNA library by using the probe containing sequence homologous in human CRP and rabbit CRP. Restriction map of this cDNA showed only one site for each following restriction enzyme EcoRI, HindIII, PstI, Sacl and Xbal. DNA sequence analysis revealed that bovine CRP cDNA has 57 bp coded for signal peptide of 19 amino acid and 618 bp for mature protein of 206 amino acid residues. The first and the last amino acid residues of the mature protein, deduced from its DNA sequence was found to be glutamine and tryptophan, …

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Production Of Monoclonal Antibodies Against Bovine C-Reactive Protein, M. Sarikaputi, W. Nunomura, M. Morimatsu, M. Naiki Sep 1993

Production Of Monoclonal Antibodies Against Bovine C-Reactive Protein, M. Sarikaputi, W. Nunomura, M. Morimatsu, M. Naiki

The Thai Journal of Veterinary Medicine

Three monoclonal antibodies (MAbs) against bovine C-reactive protein (CRP) were produced by fusing murine myeloma cell line P3X63Ag8U1 and splenic lympocytes from two BALB/c mice immunized with 100 ug of purified CRP, followed by two booster injections at 10-day intervals. The hybridomas producing antibodies specific for bovine CRP were obtained with effciencies of 1.3 % and 0.2 % respectively. All three MAbs were IgM class with k- light chains. The MAbs obtained from ascitic fluid showed definite antibody activity at 1,000 to 10,000 and the antibody concentrations were 0.9 to 1.7 mg/ml. Two of three MAbs showed significant cross-reactivity with …

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Histopathology Of Bovine Mastitis, The, C.F. Helmboldt, E.L. Jungherr, W.N. Plastridge Dec 1953

Histopathology Of Bovine Mastitis, The, C.F. Helmboldt, E.L. Jungherr, W.N. Plastridge

Storrs Agricultural Experiment Station

No abstract provided.

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Feeding And Care Of Calves, R. R. Thalman Aug 1938

Feeding And Care Of Calves, R. R. Thalman

Nebraska Agricultural Experiment Station: Historical Circulars

It is not infrequent that considerable difficulty is encountered in raising calves with limited amounts of milk or none at all. Requests for information on calf gruels, mixed grain feeds, and commercial supplements are frequent enough to make a short circular upon this subject seem desirable. Furthermore, as the more diversified agricultural program gets under way these requests are increasmg.

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White Scours Of Calves, L. Van Es May 1933

White Scours Of Calves, L. Van Es

Nebraska Agricultural Experiment Station: Historical Circulars

It becomes possible to understand some of the reasons for the occurrence of disorders among the newborn of animals kept under the usual conditions imposed by domestication. Among these, the subject of this circular, White Scours in Calves, occupies a prominent place.

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