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2023

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Articles 331 - 360 of 373

Full-Text Articles in Veterinary Medicine

Investigation Of Relationships Between Serum Lactate, Acute Phase Proteins, Pro/ Antiinflammatory Cytokine Levels, And Metritis Formation In Holstein Dairy Heifers, Talha Avcilar, Davut Koca, Yavuz Nak, Mustafa Eren Şahi̇n, Musa Özgür Özyi̇ği̇t, Deni̇z Nak Jan 2023

Investigation Of Relationships Between Serum Lactate, Acute Phase Proteins, Pro/ Antiinflammatory Cytokine Levels, And Metritis Formation In Holstein Dairy Heifers, Talha Avcilar, Davut Koca, Yavuz Nak, Mustafa Eren Şahi̇n, Musa Özgür Özyi̇ği̇t, Deni̇z Nak

Turkish Journal of Veterinary & Animal Sciences

The main purpose of this investigation was to identify and suggest suitable early screening inflammatory markers for the early diagnosis of uterine infections such as acute septic metritis (ASM) in the Holstein heifers. In addition, to achieve the above -mentioned aim, the objectives of the present study were tried to determine the relationship between blood serum lactate, acute phase proteins (APPs), pro/anti inflammatory cytokine levels, and metritis formation in Holstein heifers. For this purpose, 250 Holstein heifers which were 11-13 months old, were chosen as the study material. After heifers were conceived, blood serum samples were collected at the 8th …

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Effect Of Different Light Intensities And Colors On Growth Performance And Reproductive Characteristics In Japanese Quails, Fawwad Ahmad, Muhammad Sharif, Muhammad Ashraf, Muhammad Riaz, Muhammad Shoaib, Talha Siddique, Aqian Ahmed, Ahmad Hamad Sheir, Muhammad Ahsan Yaseen, Muhammad Mahboob Ali Hamid Jan 2023

Effect Of Different Light Intensities And Colors On Growth Performance And Reproductive Characteristics In Japanese Quails, Fawwad Ahmad, Muhammad Sharif, Muhammad Ashraf, Muhammad Riaz, Muhammad Shoaib, Talha Siddique, Aqian Ahmed, Ahmad Hamad Sheir, Muhammad Ahsan Yaseen, Muhammad Mahboob Ali Hamid

Turkish Journal of Veterinary & Animal Sciences

Light is one of the most important environmental factors in quail farming, as it has a significant impact on production efficiency and reproductive characteristics. The purpose of this study was to determine the effect of different light intensities and colors on production performance in Japanese quails. For the first experiment, 200 Japanese quails were divided into five treatment groups, each with four replicates (10 birds in each). The birds in the control treatment were exposed to a light intensity of 20 lux, while the other four treatment groups were exposed to light intensities of 10 lux, 15 lux, 25 lux, …

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Arterial Vascularization Of The Spleen In Merino Sheep, Melek Tosun, Şükrü Hakan Atalgin Jan 2023

Arterial Vascularization Of The Spleen In Merino Sheep, Melek Tosun, Şükrü Hakan Atalgin

Turkish Journal of Veterinary & Animal Sciences

In this study, it is aimed to reach detailed information about the anatomy of arteries in the sheep spleen, which can be used as an example and model for the success of planned or emergency surgical interventions related to the spleen. In spleen injuries, the spleen may be divided into two or three parts, or it may be limited to cracks on the spleen. In particular, it is aimed to protect the organs that have been damaged by trauma recently. Operations such as splenectomy and splenography are surgical procedures that require good knowledge of the pathways of artery and its …

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Examination Of Canine Herpesvirus-1 In Dogs By Serological, Molecular, And Pathological Methods, Serkan Yildirim, Fatma Gülten Bayraktar, Hakan Aydin, Yavuz Seli̇m Sağlam, Gi̇zem Eser, Muhammet Bahaeddi̇n Dörtbudak Jan 2023

Examination Of Canine Herpesvirus-1 In Dogs By Serological, Molecular, And Pathological Methods, Serkan Yildirim, Fatma Gülten Bayraktar, Hakan Aydin, Yavuz Seli̇m Sağlam, Gi̇zem Eser, Muhammet Bahaeddi̇n Dörtbudak

Turkish Journal of Veterinary & Animal Sciences

In this study, it was aimed to examine Canine Herpesvirus-1 (CHV-1) with different diagnostic methods and investigate the pathological changes in positive samples. For this purpose, 100 dog uteruses of different ages and breeds, were removed by ovariohysterectomy and their blood samples were used as the study material. Enzyme-linked immunosorbent assay (ELISA) and Indirect Fluorescent Antibody Test (IFAT) for serological diagnosis, polymerase chain reaction (PCR) method for molecular diagnosis, immunohistochemistry (IHC) and immunofluorescence (IF) staining for immunopathological diagnosis were performed on the samples. Additionally, hematoxylin and eosin (HE) staining was performed to examine the histopathological changes in the CHV- 1-positive …

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Genetic Parameters Of Growth, Reproductive, And Productive Characteristics In Anatolian Buffaloes, Yusuf Kaplan, Mustafa Tekerli̇ Jan 2023

Genetic Parameters Of Growth, Reproductive, And Productive Characteristics In Anatolian Buffaloes, Yusuf Kaplan, Mustafa Tekerli̇

Turkish Journal of Veterinary & Animal Sciences

It was aimed to investigate the genetic parameters in growth, fertility, and productivity of Anatolian buffaloes maintained at private farms of Yozgat. A total of 1139 buffalo cows and their calves were used in the study. The weights at birth, weaning, 6th and 12th months of age, and daily live weight gains (DLWG) of buffalo calves born from 2015 to 2019 were recorded. Calving interval (CI) and service period (SP), lactation milk yield (LMY), milk yield per day of lactation period (MYPDLP), milk yield per day of calving interval (MYPDCI), peak yield (PY), a day at peak yield (DPY) and …

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Modeling Of Individual Egg Weights Of Lohmann-Brown Layer Hens, Esra Yavuz, Samet Hasan Abaci, Kadi̇r Erensoy, Mustafa Şahi̇n Jan 2023

Modeling Of Individual Egg Weights Of Lohmann-Brown Layer Hens, Esra Yavuz, Samet Hasan Abaci, Kadi̇r Erensoy, Mustafa Şahi̇n

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to determine the most suitable model for egg weights of the Cubic, Gompertz, Logistics, Gamma, Richard, Piecewise Quadratic, Orscov, and Sigmoaidal models, which are widely used in estimation. Lohmann-Brown Classic laying hens raised in Ondokuz Mayıs University Research and Application farm were used as animal material. In the modeling of egg weights of 351 layer hens raised in 3-storey cages, individual egg weight data measured at weekly intervals, 32 on the 1st floor, 17 on the 2nd floor, and 27 on the 3rd floor, were taken into account, and a total of 76 hens' individual …

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The Effect Of Simvastatin Treatment On Tnf-Alpha-Induced Inflammation In Human Vascular Endothelial Cells (Huvecs), Büşra Görgün, Betül İşi̇ner Kaya, Kübra Kaya, Leyla Di̇dem Kozaci Jan 2023

The Effect Of Simvastatin Treatment On Tnf-Alpha-Induced Inflammation In Human Vascular Endothelial Cells (Huvecs), Büşra Görgün, Betül İşi̇ner Kaya, Kübra Kaya, Leyla Di̇dem Kozaci

Turkish Journal of Veterinary & Animal Sciences

This study investigates the effects of simvastatin (SMV) on human umbilical vein endothelial cells (HUVECs) inflamed by tumor necrosis factor-alpha (TNF-a). Cells were treated with simvastatin (5 μM, 10 μM, and 50 μM) with/without TNF (20 ng/mL). The effects of simvastatin on the proliferation of cells were examined, and its cytotoxic effect was determined by lactate dehydrogenase (LDH) assays. Metalloproteinase expression (MMP-2 and MMP-9) levels of SMV at different concentrations were determined by qPCR, and protein levels were determined by Western blot. The increasing concentrations of SMV down-regulated the expression levels of MMPs. After stimulation with TNF-a, the effect of …

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Effects Of Feeding In Different Quality Rangelands On Meat Quality And Fatty Acids Composition In Angora Goat Kids And Young Bucks, Betül Zehra Sariçi̇çek Jan 2023

Effects Of Feeding In Different Quality Rangelands On Meat Quality And Fatty Acids Composition In Angora Goat Kids And Young Bucks, Betül Zehra Sariçi̇çek

Turkish Journal of Veterinary & Animal Sciences

The effects of feeding in different quality rangelands on meat quality and fatty acids composition in Angora goat kids and bucks were determined. Twenty male Angora goat kids were selected from each farm that produces Angora goats in the area. There are three separate rangelands with previously determined quality classes (good, medium, and low-quality). In each farm, among these animals grazed completely on the rangeland, 7 heads from 4-month-old kids and 7 heads from 7-month-old bucks were selected and slaughtered. The Longissimus thoracis muscle was removed for meat quality measurement. The increased rangeland quality increased the protein content of kid's …

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The First Record Of Philophthalmus Lucipetus (Trematoda: Philophthalmidae) In The Great Egret (Ardea Alba) From Turkey, Mehmet Öztürk, Şi̇nasi̇ Umur Jan 2023

The First Record Of Philophthalmus Lucipetus (Trematoda: Philophthalmidae) In The Great Egret (Ardea Alba) From Turkey, Mehmet Öztürk, Şi̇nasi̇ Umur

Turkish Journal of Veterinary & Animal Sciences

The genus Philophthalmus, Looss 1899 (Echinostoma: Philophthalmidae) is a cosmopolitan group of parasites that infects the eyes of birds and mammals, including humans. A great egret (Ardea alba) was found dead in Kızılırmak Delta, Samsun, Turkey. The bird was transported to the Faculty of Veterinary Medicine, Ondokuz Mayıs University, Samsun Turkey, for a necropsy. In the parasitological examination, a trematode was detected in the eye, and then it was identified as Philophthalmus lucipetus. This is the first report of the zoonotic parasite P. lucipetus in Turkey.

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Investigation Of The In Vitro Antioxidant Activity Of Fermented Tomato Pruning Residues And Its Effects On Biochemical And Hematological Parameters Of Rats, Bariş Denk, Beyza Aydoğan, İsmai̇l Bayram, Abdurrahman Fati̇h Fi̇dan Jan 2023

Investigation Of The In Vitro Antioxidant Activity Of Fermented Tomato Pruning Residues And Its Effects On Biochemical And Hematological Parameters Of Rats, Bariş Denk, Beyza Aydoğan, İsmai̇l Bayram, Abdurrahman Fati̇h Fi̇dan

Turkish Journal of Veterinary & Animal Sciences

In the tomato greenhouse production process, a by-product emerges in the form of greenhouse residues. One of these residues is leaf pruning residue, which poses an environmental pollution problem in terms of waste management. The purpose of this work was to investigate the use of fermented tomato leaves (FTL) as an animal feed additive and its potential to mitigate the environmental pollution problem caused by greenhouse pruning residues. The pruning residues were fermented and their in vitro antioxidant activity (AOA) levels were analyzed using 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picryl-hydrazylhydrate (DPPH), reducing power (RP), and total phenolic content (TPC) assays. Wistar albino …

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Investigation Of Bmp15 And Gdf9 Gene Polymorphisms And Their Effects On Litter Size In Anatolian Sheep Breed Akkaraman, Koray Kirikçi Jan 2023

Investigation Of Bmp15 And Gdf9 Gene Polymorphisms And Their Effects On Litter Size In Anatolian Sheep Breed Akkaraman, Koray Kirikçi

Turkish Journal of Veterinary & Animal Sciences

This study aimed to examine the polymorphism of the BMP15 (FecX$^{G}$ and FecX$^{I}$) and GDF9 (G1 and G4) genes and the association between genotypes and litter size in the Akkaraman breed. Blood samples were taken from 100 ewes in total. Genomic DNA was obtained from whole blood. The PCR-RFLP technique was employed to determine the polymorphisms of the genes under investigation. The G1 was the only polymorphic among the four loci analyzed. The frequencies for the GA and GG genotypes were 0.26 and 0.74, respectively, while the A and G allele frequencies were 0.13 and 0.87, respectively. The observed and …

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The Effect Of Saccharomyces Cerevisiae As A Probiotic On The Nutrient Degradability Of Some Commonly Feedstuffs Used In Turkey, Muslimo Abdullahi Sheikh Hassan, Mehmet Aki̇f Karsli Jan 2023

The Effect Of Saccharomyces Cerevisiae As A Probiotic On The Nutrient Degradability Of Some Commonly Feedstuffs Used In Turkey, Muslimo Abdullahi Sheikh Hassan, Mehmet Aki̇f Karsli

Turkish Journal of Veterinary & Animal Sciences

The objective of this study was to evaluate the effects of Saccharomyces cerevisiae as a probiotic on in situ ruminal DM, OM, CP and starch degradability values in some energy and protein sources commonly used in ruminant nutrition. In this study, the in situ degradation kinetics and fractions of dry matter, organic matter, crude protein, and starch of 6 different feedstuffs (barley, wheat, corn, sunflower seed meal, cottonseed meal and soybean meal) commonly used in animal nutrition were determined. Three ruminally cannulated Bafra sheep were used in the experiment. The experiment was designed as carryover experimental design with two periods. …

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Investigation Of Snps In Bmp15 And Gdf9 Genes In "Çepni" And "Of" Sheep In The Black Sea Region Of Turkey, Koray Kirikçi Jan 2023

Investigation Of Snps In Bmp15 And Gdf9 Genes In "Çepni" And "Of" Sheep In The Black Sea Region Of Turkey, Koray Kirikçi

Turkish Journal of Veterinary & Animal Sciences

Sheep fertility genes have been studied in Turkey for many years. But so far it cannot be said that a satisfactory result has been achieved in terms of the number of detected polymorphisms on fertility genes. Exhibiting the genetic structure of local gene resources adapted to a particular region is very important for sustainability, conservation and breeding studies, and many other issues such as profitability. Therefore, the primary aim of this study was to investigate the single nucleotide polymorphisms (SNPs) on the BMP15 and GDF9 genes in two new Turkish sheep breeds, Çepni and Of. DNA sequencing was performed to …

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Influence Of Lactobacillus Plantarum Administration On Some Serum/Tissue (Uterus) Antioxidant And Cytokine Levels In Female Rats Exposed To Cadmium (Cd), İhsan Kisadere, Bariş Güner, Hakan Tavşanli, Mehmet Faruk Aydin, Mustafa Usta Jan 2023

Influence Of Lactobacillus Plantarum Administration On Some Serum/Tissue (Uterus) Antioxidant And Cytokine Levels In Female Rats Exposed To Cadmium (Cd), İhsan Kisadere, Bariş Güner, Hakan Tavşanli, Mehmet Faruk Aydin, Mustafa Usta

Turkish Journal of Veterinary & Animal Sciences

The study aimed to investigate the effects of oral Lactobacillus plantarum (Lp) administration on some serum/tissue (uterus) antioxidant and cytokine levels as well as histopathologic changes in female rats exposed chronically to Cd. For this purpose, rats were separated into four groups including control (C, n = 8), cadmium (Cd, n = 8), Lp (n = 8), and Cd + Lp (n = 8). Cd (dissolved in water at a dose of 2.04 mg/mL) was orally administered to Cd and Cd + Lp groups for 28 days. Besides, active-live Lp, which was grown at a level of approximately 10$^{8}$-10$^{9}$ cfu/mL …

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Could There Be A Relationship Between Feeding Characteristics And The Shape Of Condylus Occipitalis And Foramen Magnum In Mammals?, Yasi̇n Demi̇raslan, İsmai̇l Demi̇rci̇oğlu, İftar Gürbüz, Funda Aksünger Karaavci, Abdullah Ortadeveci, Özcan Özgel Jan 2023

Could There Be A Relationship Between Feeding Characteristics And The Shape Of Condylus Occipitalis And Foramen Magnum In Mammals?, Yasi̇n Demi̇raslan, İsmai̇l Demi̇rci̇oğlu, İftar Gürbüz, Funda Aksünger Karaavci, Abdullah Ortadeveci, Özcan Özgel

Turkish Journal of Veterinary & Animal Sciences

The aim of the study was to investigate the shape of the foramen magnum and condylus occipitalis of mammalian species belonging to different feeding groups. For this, two-dimensional geometric morphometric method analyses were applied. The data from the skulls of the 12 species were used. In principal component analysis, the first three principal components accounted for 70.353%of the total shape variation. According to the first principal component, the dorsal edge of the condylus occipitalis and foramen magnum contributed the most to shape variation. Most of the individuals were correctly separated according to the feeding groups. The most accurate grouping features …

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Molecular Detection Of Canine And Feline Hemotropic Mycoplasmas In Northern Vietnam, Bui Thi Huyen Thuong Jan 2023

Molecular Detection Of Canine And Feline Hemotropic Mycoplasmas In Northern Vietnam, Bui Thi Huyen Thuong

Chulalongkorn University Theses and Dissertations (Chula ETD)

Hemoplasma is commonly used to refer to hemotropic Mycoplasma bacterial species belonging to the genus Mycoplasma that can attach to the surface of red blood cells, potentially leading to hemolytic anemia. These organisms can infect a wide range of mammals, including companion animals and humans worldwide. Specifically for companion animals, two main hemoplasma species can infect dogs, and three major species can infect cats including Mycoplasma haemocanis, Candidatus Mycoplasma haematoparvum, Mycoplasma haemofelis, Candidatus Mycoplasma haemominutum, and Candidatus Mycoplasma turicensis, respectively. Currently, there is a lack of research data regarding hemoplasma infections in dogs and cats in Vietnam. Therefore, this study …

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Microencapsulation Of Lactobacillus Plantarum Mb001 And Its Probiotic Effect On Growth Performance And Gut Integrity Of Broile Rs, Sasi Vimon Jan 2023

Microencapsulation Of Lactobacillus Plantarum Mb001 And Its Probiotic Effect On Growth Performance And Gut Integrity Of Broile Rs, Sasi Vimon

Chulalongkorn University Theses and Dissertations (Chula ETD)

The objectives of this work were 1) to study the preparation and characterization of LPMB001/AG-AL, 2) todetermine the characteristics, stability and viability of LPMB001/AG-AL against simulated gastrointestinal tract, high temperature and storage conditions and 3) To evaluate the supplementation of LPMB001/AG-AL on growth performance,ileal digestibility, intestinal morphology and microbiome in broiler chickens. The present work declared microcapsule networks based on polymer matrices (sodium alginate, AL and agar, AG) and charges under ionic crosslink aw emulsion to encapsulate probiotics for extensive stability in adverse conditions. The microcapsule was studied on physicochemicalcharacteristics, swelling efficiency as well as release pertormance and viability under …

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Expression Of Proteins Mediating Pulmonary Hypertension In Dogs Affected With Degenerative Mitral Valve Disease, Siriwan Sakarin Jan 2023

Expression Of Proteins Mediating Pulmonary Hypertension In Dogs Affected With Degenerative Mitral Valve Disease, Siriwan Sakarin

Chulalongkorn University Theses and Dissertations (Chula ETD)

Pulmonary hypertension (PH) is defined by an abnormal elevation in pressure within pulmonary vasculature, often associated with degenerative mitral valve disease (DMVD) in dogs. While histopathological changes in the pulmonary arteries, known as pulmonary arterial remodeling, have been documented in human patients with PH, these alterations in DMVD dogs with PH have not been published. Proteomics, a potent method for exploring the entire protein complement of a biological sample, has emerged as a valuable tool for identifying PH-specific proteins, potentially serving as biomarkers for diagnosis and enhancing understanding of PH pathogenesis in DMVD dogs. This study aimed to evaluate the …

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Screening Of Lactate Utilizing Bacteria And Their Beneficial Properties To Improve Energy Balance, Production And Ruminal Fermentation In Crossbred Dairy Goats, Saranporn Poothong Jan 2023

Screening Of Lactate Utilizing Bacteria And Their Beneficial Properties To Improve Energy Balance, Production And Ruminal Fermentation In Crossbred Dairy Goats, Saranporn Poothong

Chulalongkorn University Theses and Dissertations (Chula ETD)

Sixty-three strains were isolated from ruminal fluid of crossbred dairy goats. Based on phenotype and 16S rRNA gene, sixteenbacterial groups were identified and two novel species of Selenomonas were purposed in this study. The strain mPRGC5 was purposed as Selenomonas runimis and the strain mPRGC8 was named Selenomonas caprae. The antimicrobial activity of all the strains was determined.Ligilactobacillus salivarius MP3 showed antibacterial activity against Cutibacterium acnes subsp. acnes DSM 1897' and Kocuria thizophila Mill. The antimicrobial substance was genomically identified as enterolysin A. Furtherniore, L. salivarius MP3 presented probiotic properties and the potential antimicrobial activity against pathogens. The fourteen strains …

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Application Of Anti-Müllerian Hormone As An Indicator For Ovarian Reserve And Ovarian Response To Hormonal Stimulation In Domestic Cats And Wild Felids, Sittat Chumsri Jan 2023

Application Of Anti-Müllerian Hormone As An Indicator For Ovarian Reserve And Ovarian Response To Hormonal Stimulation In Domestic Cats And Wild Felids, Sittat Chumsri

Chulalongkorn University Theses and Dissertations (Chula ETD)

Serum anti-Müllerian hormone (AMH) has been utilized to predict ovarian reserve and select suitable candidates for natural mating and artificial insemination (AI) in domestic animals. However, research on serum AMH concentration as a fertility marker in feline species remains limited. This study aims to investigate: 1) the possibility of using serum AMH levels to determine the number of follicles in domestic cats; 2) the validation of the AMH Gen II ELISA for measuring serum AMH levels in fishing cats, leopard cats and clouded leopards; and 3) the relationship between serum AMH levels and ovarian responsiveness in clouded leopards. In Study …

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Contraception In Free-Ranging Long-Tailed Macaques With Special Focus On Anesthesia Management And Development Of Nanobody Targeting Extracellular Domain Of Follicular Stimulating Hormone Receptors, Pakpoom Navanukraw Jan 2023

Contraception In Free-Ranging Long-Tailed Macaques With Special Focus On Anesthesia Management And Development Of Nanobody Targeting Extracellular Domain Of Follicular Stimulating Hormone Receptors, Pakpoom Navanukraw

Chulalongkorn University Theses and Dissertations (Chula ETD)

The overlapping presence of free-ranging long-tailed macaques (Macaca fascicularis) and humans in Thailand and ฆSoutheast Asia resulted in physical violence, ravaging and zoonotic disease transmission. To mitigate these detriments, the synergy of surgical and nonsurgical contraception is essential for imperative population control. This thesis aimed to: 1) enhance animal welfare and safety of macaques undergoing surgical contraception by applying multimodal anesthesia practices; 2) generate alternative non-surgical contraceptives against the extracellular domain (ECD) of follicular stimulating hormone receptors (FSHr) in long-tailed macaques; 3) formulate nano/microstructured microspheres containing anti-FSHr scFv for oral delivery and determine the pharmacokinetics and bioavailabilityIn Study 1, the …

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De Novo Assembly Of The Reference Genome, Genetic Variations And Serum Proteomics Of Eld's Deer (Rucervus Eldii), Vichayanee Pumpitakkul Jan 2023

De Novo Assembly Of The Reference Genome, Genetic Variations And Serum Proteomics Of Eld's Deer (Rucervus Eldii), Vichayanee Pumpitakkul

Chulalongkorn University Theses and Dissertations (Chula ETD)

Eld’s deer is a conserved wildlife species in Thailand. Due to the limited population group in captivity, Eld’s deer is facing a crisis of low genetic diversity, particularly among the captive Siamese Eld’s deer (SED) subspecies. This study aimed to construct genomes for a SED and a Burmese Eld’s deer (BED) for the first time and use genome-wide single nucleotide polymorphisms (SNPs) to assess the genetic purity and inbreeding status of 35 SED and 49 BED with limited pedigree data. Results revealed that the 2 subspecies diverged approximately 1.26 million years ago. All SED are purebred, whereas a small proportion …

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Utilization Of Testosterone-Encapsulated Nanoparticles For Masculinization Of Red Tilapia (Oreochromis Sp.), Jakarwan Yostawonkul Jan 2023

Utilization Of Testosterone-Encapsulated Nanoparticles For Masculinization Of Red Tilapia (Oreochromis Sp.), Jakarwan Yostawonkul

Chulalongkorn University Theses and Dissertations (Chula ETD)

Tilapia (Oreochromis sp.) is a freshwater fish that is highly popular for aquaculture in Thailand. To achieve high yields, good growth rates, and better feed conversion rates, 17-alpha-methyltestosterone are commonly used to induce male sex in farmed tilapia. However, it has been found in previous studies that these synthetic hormones have low water solubility and limited absorption capabilities, leading to excessive hormone use. In this research, lipid-based nanoparticles, a widely used drug delivery technology, were employed to enhance the efficiency of synthetic hormones with low water solubility. This improved the dispersion of hormones in water, enhanced their absorption in the …

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Genome Characterization And Development Of Inactivated Vaccine Against Scale Drop Disease Virus (Sddv) In Asian Sea Bass (Lates Calcarifer), Putita Chokmangmeepisarn Jan 2023

Genome Characterization And Development Of Inactivated Vaccine Against Scale Drop Disease Virus (Sddv) In Asian Sea Bass (Lates Calcarifer), Putita Chokmangmeepisarn

Chulalongkorn University Theses and Dissertations (Chula ETD)

Scale drop disease virus (SDDV) is a significant pathogen affecting Asian sea bass (Lates calcarifer) in Southeast Asia, causing scale drop syndrome (SDS) and resulting in high mortality and substantial economic losses in aquaculture. Recently, SDDV has been reported in Asian sea bass farms throughout the Asia-Pacific region including Thailand, Indonesia, Malaysia, Singapore, and China. This study employed metagenomic methods to analyze bacterial and viral communities in infected fish tissues from Southeast Asia, leading to the recovery and characterization of the complete genome of SDDV strain TH7_2019. The genome, comprising 131 kb with 134 putative ORFs, showed high amino acid …

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Characterization Of Antimicrobial Resistance And Virulence Factors In Multidrug-Resistant Extraintestinal Pathogenic Escherichia Coli Isolated From Canine Urinary Tract, Reproductive Tract And Bloodstream Infections, Parinya Sroithongkham Jan 2023

Characterization Of Antimicrobial Resistance And Virulence Factors In Multidrug-Resistant Extraintestinal Pathogenic Escherichia Coli Isolated From Canine Urinary Tract, Reproductive Tract And Bloodstream Infections, Parinya Sroithongkham

Chulalongkorn University Theses and Dissertations (Chula ETD)

Despite extensive characterization of uropathogenic Escherichia coli (UPEC) causing urinary tract infections (UTIs), the genetic background of non-urinary extraintestinal pathogenic E. coli (ExPEC) in dogs, such as those causing canine pyometra, prostatic abscesses (PAs), and bloodstream infections (BSIs), remains inadequately understood. This study characterized the virulence determinants of 101 E. coli isolates from canine pyometra (n=61), PAs (n=38), and BSIs (n=2). The analysis revealed a stronger association of pyometra strains with UPEC compared to PA strains. Forty-one isolates were resistant to third-generation cephalosporins and/or fluoroquinolones, with 14 being extended-spectrum β-lactamase producers and carrying blaCTX-M. Whole-genome sequencing was performed on 10 …

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Development And Evaluation The Efficacy Of A Water-Soluble Probiotic Microencapsulation Prototype For Pediococcus Acidilactici Isolated From Broiler Chickens, Ratchnida Kamwa Jan 2023

Development And Evaluation The Efficacy Of A Water-Soluble Probiotic Microencapsulation Prototype For Pediococcus Acidilactici Isolated From Broiler Chickens, Ratchnida Kamwa

Chulalongkorn University Theses and Dissertations (Chula ETD)

Probiotics are live bacteria that can be given orally to promote gastrointestinal health. A limitation of probiotic use is their diminished efficiency following the loss of live cells during high-temperature processing and storage. Microencapsulation technology has been created to protect probiotic cells, preserve their viability, and maintain their efficacy following manufacture. The aim of this study was to evaluate the effectiveness of a water-soluble microencapsulation method using gum Arabic (GA) and skim milk (SKM) over a three-month storage period following processing. Four strains of Pediococcus acidilactici (BYF26, BYF20, BF9, and BF14) that were typical lactic acid bacteria (LAB) strains isolated …

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Novel Vaccination Strategy Against Vibrio Harveyi Infection In Asian Seabass (Lates Calcarifer) Using A Chitosan – Oxygen Nanobubble - Vaccine Complex, Nguyen Giang Thu Lan Jan 2023

Novel Vaccination Strategy Against Vibrio Harveyi Infection In Asian Seabass (Lates Calcarifer) Using A Chitosan – Oxygen Nanobubble - Vaccine Complex, Nguyen Giang Thu Lan

Chulalongkorn University Theses and Dissertations (Chula ETD)

Vibriosis is a bacterial disease caused by Vibrio harveyi (V. harveyi; Vh ) that poses a substantial threat to Asian seabass (Lates calcarifer) aquaculture. In this study, we investigated alternative approaches to conventional injection vaccination for prevention of V. harveyi in Asian seabass. The first approach involved an immersion prime with heat-inactivated V. harveyi vaccine, followed by two oral booster doses (day 14-18 and day 28-32). Immunized fish demonstrated higher expression levels of immune genes (i.e. TNFα, IL1β, CD4, CD8, and IgM) following the immersion prime and the first oral booster dose, peaking at 14 and 28 days post vaccination …

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Farrowing Performances And Piglet Characteristics In Highly Prolific Sows In Tropical Environment: Impact Of Aging, Ambient Temperature And Humidity Within The Barn, And Feeds, Yosua Kristian Adi Jan 2023

Farrowing Performances And Piglet Characteristics In Highly Prolific Sows In Tropical Environment: Impact Of Aging, Ambient Temperature And Humidity Within The Barn, And Feeds, Yosua Kristian Adi

Chulalongkorn University Theses and Dissertations (Chula ETD)

Hyperprolific sows have been widely distributed in the global swine industry, including Thailand. Therefore, factors affecting the farrowing performance and piglet characteristics of these sows were investigated to establish effective management practices, especially in tropical climates. Moreover, strategies to enhance sow colostrum production and expedite the farrowing process were also explored. Additionally, deep insight into the effect of aging on farrowing performance, particularly farrowing duration, through investigation of reproductive organ morphology and oxytocin receptor expression on uterine tissue was conducted. The study revealed that sows with a high number of piglets born per litter, a high parity number, and parturition …

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Assessment Of Longitudinal Systolic Funcion By Using Tissue Motion Annular Displacement In Hypertrophic Cardiomyopathy Cats, Nadthagarn Glaewketgarn Jan 2023

Assessment Of Longitudinal Systolic Funcion By Using Tissue Motion Annular Displacement In Hypertrophic Cardiomyopathy Cats, Nadthagarn Glaewketgarn

Chulalongkorn University Theses and Dissertations (Chula ETD)

In humans, tissue motion annular displacement (TMAD) providesresponsive and accurate assessment on longitudinal systolic function. The aim ofthis study is to investigate the LV longitudinal systolic function in hypertrophiccardiomyopathy (HCM) and control cats by using TMAD. The study includes 26control cats and 21 HCM cats. Global TMAD were not influence by breed, sex, age,and heart rate. Global TMAD and % Global TMAD significantly decreased in theHCM group (2.86±0.86 mm and 11.46±3.37%) when compared to control group(3.95±0.89 mm and 16.12±2.94%) (p<0.001 and <0.001), suggesting LV longitudinalsystolic dysfunction in HCM cats. LV fractional shortening (LVFS) showed nodifference between the control (52.76±11.63%) and HCM stage group(59.80±13.51%) (p=0.061). Indicate that TMAD is a sensitive technique foridentifying LV longitudinal systolic dysfunction. Global TMAD and % global TMADhad a significant correlation with global longitudinal strain (GLS). Therefore, TMADcan be used to evaluate longitudinal systolic function in HCM. The reliability test ofglobal TMAD and % global TMAD were considered moderate. In conclusion, globalTMAD and % global TMAD was significantly decreased in cats with HCM comparedwith the control group and was considered as sensitive and repeatable techniquefor evaluating LV longitudinal systolic dysfunction in cats with HCM

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Investigating Of Candidate Genes Affecting Milk Production In Dairy Cattle Using Systematic Review And Whole Genome Sequencing Database, Xiaoxian Su Jan 2023

Investigating Of Candidate Genes Affecting Milk Production In Dairy Cattle Using Systematic Review And Whole Genome Sequencing Database, Xiaoxian Su

Chulalongkorn University Theses and Dissertations (Chula ETD)

Milk production traits in dairy cattle, such as milk yield, fat content, and protein levels, are pivotal to the economic success of the dairy industry. These traits not only determine the quality and quantity of milk produced but also directly impact the profitability of dairy operations. Consequently, a deep understanding of the genetic architecture underlying these traits is essential for the development of effective breeding programs aimed at enhancing milk production and quality. The purpose of this study is to identify potential candidate genes related to milk production in Thai dairy cattle through systematic review of genome wide association study …

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