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Veterinary Medicine Commons

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2023

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Articles 181 - 210 of 373

Full-Text Articles in Veterinary Medicine

Comparison Of Semi-Confined And Pasture-Based August Calving Beef Cow Systems, Hannah F. Speer, Harvey C. Freetly, M. E. Drewnoski Jan 2023

Comparison Of Semi-Confined And Pasture-Based August Calving Beef Cow Systems, Hannah F. Speer, Harvey C. Freetly, M. E. Drewnoski

Nebraska Beef Cattle Reports

Limited pasture availability and increased pasture rental rates have generated a need to evaluate alternative cow-calf production systems. The current study compared cow and calf performance in two August-calving cow systems that combined corn residue grazing with 1) perennial forage grazing and hay or 2) summer drylot feeding and fall cover crop grazing. Differences in pregnancy rates between systems within year were not observed; however, the effect of production system on cow body condition and calf body weight at different time points varied across years. Overall, cow and calf performance were not negatively impacted in the drylot/cropland system, suggesting that …


Impact Of Planning Horizon Length On Breeding Objectives And Resulting Selection Decisions, Hunter F. Valasek, Bruce L. Golden, Matthew L. Spangler Jan 2023

Impact Of Planning Horizon Length On Breeding Objectives And Resulting Selection Decisions, Hunter F. Valasek, Bruce L. Golden, Matthew L. Spangler

Nebraska Beef Cattle Reports

The weighting of traits in a selection index depends on varying circumstances that can difer among beef producers (e.g. economic factors, breeding systems). The determination of planning horizon is an additional variable that can differ among producers that represents the impact of genetic selection decisions over a gradient of time. A web-based economic index construction platform (iGENDEC) was used in the current study to investigate the implications of planning horizon on relative emphasis values of traits within the breeding goal and potential differences in sire selection decisions. General-purpose indexes were created for three breeding systems under six different …


Interspecific Oral Rabies Vaccine Bait Competition In The Southeast United States, Wesley C. Dixon, Jacob E. Hill, Richard Chipman, Amy J. Davis, Amy Gilbert, James C. Beasley, Olin E. Rhodes Jr., Guha Dharmarajan Jan 2023

Interspecific Oral Rabies Vaccine Bait Competition In The Southeast United States, Wesley C. Dixon, Jacob E. Hill, Richard Chipman, Amy J. Davis, Amy Gilbert, James C. Beasley, Olin E. Rhodes Jr., Guha Dharmarajan

United States Department of Agriculture Wildlife Services: Staff Publications

The United States Department of Agriculture’s National Rabies Management Program (NRMP) has coordinated the use of oral rabies vaccination (ORV) to control the spread of raccoon rabies virus variant west of the Appalachian Mountains since 1997. Working with state and local partners, the NRMP deploys ORV baits containing a rabies vaccine, primarily targeting raccoon populations (Procyon lotor). Bait competition between raccoons and non-target species may limit the effectiveness of ORV programs, but the extent of bait competition remains poorly quantified, particularly in the southeastern United States. We placed placebo ORV baits in bottomland hardwood (n = 637 baits) and upland …


Molecular Characterization And Biosynthesis Of Izumo1 And Juno In Domestic Cats (Felis Catus), Phakjira Sanguansook Jan 2023

Molecular Characterization And Biosynthesis Of Izumo1 And Juno In Domestic Cats (Felis Catus), Phakjira Sanguansook

Chulalongkorn University Theses and Dissertations (Chula ETD)

The negative impacts of domestic cat overpopulation and the limitations of currently available methods to induce infertility in cats necessitate the development of novel targets for non-surgical contraception in this species. The recent discovery of IZUMO1-JUNO, a pair of sperm-oocyte proteins that are both indispensable for the fertilization process, inspires the targeting of them for contraception in cats. To gain insight into feline IZUMO1 and JUNO, this thesis aims to identify 1) the target organs expressing IZUMO1 and JUNO in domestic cats 2) the expression of feline IZUMO1 during epididymal sperm transit in the epididymis and feline JUNO during various …


Corneal Characterization Of Canine Keratoconjunctivitis Sicca By Optical Coherence Tomography, Kedsaraporn Ruangpanitchayakul Jan 2023

Corneal Characterization Of Canine Keratoconjunctivitis Sicca By Optical Coherence Tomography, Kedsaraporn Ruangpanitchayakul

Chulalongkorn University Theses and Dissertations (Chula ETD)

Keratoconjunctivitis sicca (KCS), known as dry eye syndrome, indicates an imbalance in tear film stability and homeostasis. Quantitative KCS is well diagnosed while qualitative KCS is still underdiagnosed in routine veterinary practice. This study aimed to compare the ocular surface characteristics between experimental dogs (n=20) presented with low Schirmer tear test (STT) values (1-10mm/min) and control dogs (n=20) with normal STT values (≥15mm/min). Dogs with previous treatments or diseases which effect tear production were excluded. The experimental group showed significant increased KCS-related clinical signs score with mucopurulent ocular discharge, keratitis with corneal edema and vessels, and conjunctivitis with hyperemia and …


Occurrence Of Staphylococcus Aureus In Milk And Antibacterial Properties Of Some Herbal Extracts On Milk And Hospital-Acquired Strains, Reza Ebrahimi Hariry, Gülçi̇n Akca, Ayhan Fi̇lazi̇ Jan 2023

Occurrence Of Staphylococcus Aureus In Milk And Antibacterial Properties Of Some Herbal Extracts On Milk And Hospital-Acquired Strains, Reza Ebrahimi Hariry, Gülçi̇n Akca, Ayhan Fi̇lazi̇

Turkish Journal of Veterinary & Animal Sciences

Staphylococcus aureus is a remarkable infectious agent, and with the development of antibiotic resistance by S. aureus, finding new, effective, and safe alternative therapeutic options in animals and humans is crucially demanded. In this study, the occurrence of S. aureus was explored by collecting nonmastitis bovine milk samples first, and then the effects of herbal extracts on methicillinresistant S. aureus (MRSA) strains isolated from cow's milk and humans in the hospitals were investigated. The occurrences of S. aureus in bovine raw milk samples were 13.4%. All raw milk S. aureus isolates were fully susceptible to cefoxitin, ceftiofur, penicillin G, …


Rectal Temperature And Respiration Rate As Indicators Of Heat Stress In Broiler Chickens Subjected To Early-Age Thermal Conditioning And Vitamin C Supplementation, Zoran Ruzic, Zdenko Kanacki, Slobodan Stojanovic, Zorana Kovacevic, Slobodan Knezevic, Srdan Todorovic, Smiljana Paras Jan 2023

Rectal Temperature And Respiration Rate As Indicators Of Heat Stress In Broiler Chickens Subjected To Early-Age Thermal Conditioning And Vitamin C Supplementation, Zoran Ruzic, Zdenko Kanacki, Slobodan Stojanovic, Zorana Kovacevic, Slobodan Knezevic, Srdan Todorovic, Smiljana Paras

Turkish Journal of Veterinary & Animal Sciences

One of the major concerns in poultry production presents heat stress (HS) and it is followed by significant economic loss manifested by reduced growth, decreased immune response, increased mortality, etc. The objective of this study was to investigate whether the early-age thermal conditioning (ETC) and vitamin C (Vit.C) treatments, individually and in combination, produce their beneficial effects through increased heat release manifested as a change in respiration rate consequentially leading to a decrease in the chicken's body temperature and thus reduce the harmful effects of HS. A 400 broiler chicks mixed sex male and female were split into four groups. …


Effects Of Plasma Sample Handling On Equine Insulin Assays, Lauren Willett Jan 2023

Effects Of Plasma Sample Handling On Equine Insulin Assays, Lauren Willett

Murray State Theses and Dissertations

Often, the assumption is made that the results of the various laboratory tests being run are accurate, and that abnormal results reflect a physiologic change occurring in the patient. Unfortunately, this assumption is not always true. Inaccurate results, for various reasons, are an inherent part of diagnostic testing. Having inaccurate results is often worse than having no results at all, because they can lead to an incorrect diagnosis or result in unnecessary testing, wasting the client’s financial resources, or potentially expose the patient to unnecessary risks in pursuit of the cause of an abnormality that does not exist. With appropriate …


Using Rose Pulp Silage Instead Of Sugar Beet Pulp Silage In Lambs Fed With Total Mixed Ration, Kadi̇r Emre Buğdayci, Mustafa Numan Oğuz, Hale Seçi̇lmi̇ş Canbay, Hidir Gümüş, Fatma Karakaş Oğuz Jan 2023

Using Rose Pulp Silage Instead Of Sugar Beet Pulp Silage In Lambs Fed With Total Mixed Ration, Kadi̇r Emre Buğdayci, Mustafa Numan Oğuz, Hale Seçi̇lmi̇ş Canbay, Hidir Gümüş, Fatma Karakaş Oğuz

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the effects of using rose pulp (Rosa damacena) silage instead of sugar beet pulp silage on fattening performance, carcass yield, and some ruminal fluid parameters in lambs fed with total mixed ration. For this purpose, a total of 16 (average 30 ± 5 kg) 6-month-old Pırlak lambs, 8 in each group, were randomly divided into two groups. Total mixed rations were formulated by using concentrate, wheat straw and rose pulp silage (RPS) (Group 1) or sugar beet pulp silage (SBPS) (Group 2) in the experiment. Due to the low dry …


New Insights On Epidemiology Of Infectious Bronchitis Virus In Iran By Comparing Two Genotyping Methods, Arash Ghalyanchilangeroudi, Hossein Hosseini, Zahra Ziafati Kafi, Naser Sadri, Ali Hojabr Rajeoni, Hamideh Najafi Jan 2023

New Insights On Epidemiology Of Infectious Bronchitis Virus In Iran By Comparing Two Genotyping Methods, Arash Ghalyanchilangeroudi, Hossein Hosseini, Zahra Ziafati Kafi, Naser Sadri, Ali Hojabr Rajeoni, Hamideh Najafi

Turkish Journal of Veterinary & Animal Sciences

Different genotypes of infectious bronchitis virus (IBV) in poultry are circulating worldwide. The most efficient classification system for IBV genotypes is based on the complete sequencing of S1 gene, and the target organ can be trachea, kidney, oviduct, lung, esophagus, proventriculus, intestine, liver, spleen, bursa, cecal tonsils, cloaca, and testis. In Iran, IBV genotyping is usually performed by partial sequencing of S1 gene, and the trachea is often selected for sampling. This study applies a different genotyping method, and compares the results with the routine method of genotyping. Samples were collected from the trachea and kidneys of 50 broiler flocks …


Effects Of Use Of Conventional And Sexed Semen On Conception Rate, Calf Sex, Calf Birth Weight, And Stillbirth In Holstein Heifers, Tahi̇r Bayril Jan 2023

Effects Of Use Of Conventional And Sexed Semen On Conception Rate, Calf Sex, Calf Birth Weight, And Stillbirth In Holstein Heifers, Tahi̇r Bayril

Turkish Journal of Veterinary & Animal Sciences

Pregnancy rate is low in artificial insemination with sexed semen compared to conventional semen. This situation poses a major problem for dairy farms. The aim of this study was to determine the effects of semen type on conception rate, calf sex and birth weight, stillbirth, pregnancy loss, and postpartum maternal body weight (MBW) in Holstein heifers inseminated with sexed (n = 229), and conventional semen (n = 153). The female and male calf ratios of the heifers inseminated with sexed and conventional semen were found to be 88% to 54.8% and 12% to 45.2%, respectively. There was a significant difference …


Effects Of Free Capric Acid, Lauric Acid, And Coconut Oil Supplementation On Performance, Carcass, And Some Blood Biochemical Parameters Of Broiler Chickens, Mehmet Demi̇rci̇, Şevket Evci̇, Mehmet Aki̇f Karsli, Ali̇ Şenol Jan 2023

Effects Of Free Capric Acid, Lauric Acid, And Coconut Oil Supplementation On Performance, Carcass, And Some Blood Biochemical Parameters Of Broiler Chickens, Mehmet Demi̇rci̇, Şevket Evci̇, Mehmet Aki̇f Karsli, Ali̇ Şenol

Turkish Journal of Veterinary & Animal Sciences

In this study, it was aimed to investigate the effects of the addition of free capric (decanoic, C10:0) and lauric (dodecanoic, C12:0) acids and coconut (Cocos nucifera) oil to the broiler diets on performance, carcass yield, internal organ weights and some blood parameters. A total of 144 day-old broiler chicks (Ross 308) were used in the study, and four main groups were formed with four subgroups containing equal number of chicks. The control (C) group was fed with basal diet without any supplementation and the experimental groups were fed with 0.4% free capric acid (CA)-, lauric acid (LA)-, …


Taxonomic Classification Of Viral And Bacterial Dna Following 2021 Avian Mass Mortality Event, Tessa Baillargeon Jan 2023

Taxonomic Classification Of Viral And Bacterial Dna Following 2021 Avian Mass Mortality Event, Tessa Baillargeon

Honors Theses and Capstones

From May through July 2021, an unusual mortality event occurred along the eastern coast and Midwest of the United States. Thousands of birds, mostly from the order Passeriformes, were part of the die-off including blue jays (Cyanocitta cristata), common grackles (Quiscalus quiscula), European starlings (Sturnus vulgaris), American robins (Turdus migratorius). Clinical signs included crusted eyes, swollen conjunctiva, otitis, seizures, and ataxia.

The New Hampshire Veterinary Diagnostic Laboratory (NHVDL) received over 100 affected birds from various collaborators throughout the United States including Washington DC, NJ, CT, MD, and OH. Given the timing and geologic …


Pharmacokinetics And Pharmacodynamics Of Intranasal And Intramuscular Administration Of Naloxone In Working Dogs Administered Fentanyl, Ciara A Barr, Joanne Haughan, Giacomo Gianotti, Kelley Varner, Kenneth J Drobatz, Darko Stefanovski, Mary Robinson, Mark Pennington, Amanda Mcguire, Cynthia M Otto Jan 2023

Pharmacokinetics And Pharmacodynamics Of Intranasal And Intramuscular Administration Of Naloxone In Working Dogs Administered Fentanyl, Ciara A Barr, Joanne Haughan, Giacomo Gianotti, Kelley Varner, Kenneth J Drobatz, Darko Stefanovski, Mary Robinson, Mark Pennington, Amanda Mcguire, Cynthia M Otto

Faculty, Staff and Student Publications

BACKGROUND: Working dogs exposed to narcotics might require reversal in the field.

OBJECTIVE: To explore the pharmacokinetic and pharmacodynamic effects of naloxone administered intramuscularly (IM) or intranasally (IN) to reverse fentanyl sedation in working dogs.

ANIMALS: Ten healthy, working dogs aged 1.7 ± 1 year and weighing 26 ± 3 kg.

METHODS: In this randomized, controlled cross-over study dogs received either 4 mg of naloxone IN or IM 10 minutes after fentanyl (0.3 mg IV) administration. Sedation was assessed at baseline and 5 minutes after fentanyl administration, then at 5, 10, 15, 20, 25, 30, 60 and 120 minutes after …


Presumptive Hepatogenous Photosensitization In Beef Cattle Winter Grazing A Cover Crop Containing Brassica Spp. Turnips, Matthew M. Hille, Scott A. Fritz, Breanna C. Brown Jan 2023

Presumptive Hepatogenous Photosensitization In Beef Cattle Winter Grazing A Cover Crop Containing Brassica Spp. Turnips, Matthew M. Hille, Scott A. Fritz, Breanna C. Brown

School of Veterinary and Biomedical Sciences: Faculty Publications

Over a period of ~2 wk, 5 adult beef cows developed severe necrotizing skin lesions on the face and neck shortly after being moved to a field with a cover crop of Brassica spp. turnips. We describe here the clinical signs, hematology, and serum chemistry results, as well as gross and histopathologic findings, from this outbreak. We made a presumptive diagnosis of Brassica-associated liver disease (BALD) based on the similarities in the history and diagnostic findings observed with previously reported cases of BALD from elsewhere in the world. BALD has not been reported previously in cattle in North America, …


Entry Assessment Of Salmonella And E. Coli Contamination In Poultry Feed On The Farms, Ilham Ardiansah Jan 2023

Entry Assessment Of Salmonella And E. Coli Contamination In Poultry Feed On The Farms, Ilham Ardiansah

Chulalongkorn University Theses and Dissertations (Chula ETD)

This research was aimed at precisely estimating the probabilities associated with the presence of Salmonella and E .coli contamination in feed at the entry of broiler and broiler breeder farms. The samples were taken from four different farms during six weeks located in Chonburi Province, Thailand. The microbiological analysis was conducted in the Laboratory of Microbiology, Regional Medical Science Center, Saraburi, Thailand. The entry assessment followed the concept of import risk analysis, including determining models for probabilistic prevalence and the probability of entry. The statistical model used a beta distribution followed by Monte Carlo simulation to assess probabilistic prevalence and …


A Comparative Study Of Oocyte Between Native Cattle And Buffalo Species In Bangladesh: A Qualitative And Quantitative Approach, Zannatul Naim, Md Saiful Islam, Lam Yea Asad, Md Enayet Kabir Jan 2023

A Comparative Study Of Oocyte Between Native Cattle And Buffalo Species In Bangladesh: A Qualitative And Quantitative Approach, Zannatul Naim, Md Saiful Islam, Lam Yea Asad, Md Enayet Kabir

Turkish Journal of Veterinary & Animal Sciences

In vitro embryo production (IVP) can help alleviating reproductive difficulties in cattle and buffalo. This study compared qualitative and quantitative features of native cattle and buffalo ovaries and oocytes to understand them comprehensively. In addition, it was conducted to pick the ideal ovaries for oocyte collection and to assess either maturation affects oocyte diameter or not. The ovaries were collected from the local slaughterhouses of Dhaka city, Bangladesh. Next, the quantitative parameters of ovaries were measured, the follicular fluid was aspired, the oocytes were classified, and the diameters of oocytes were determined in both pre and postmaturation. Here, the results …


Development Of A Chitosan Nano-Immersion Vaccine Against Aeromonas Veronii Infection In Red Tilapia (Oreochromis Sp.), Pimwarang Sukkarun Jan 2023

Development Of A Chitosan Nano-Immersion Vaccine Against Aeromonas Veronii Infection In Red Tilapia (Oreochromis Sp.), Pimwarang Sukkarun

Chulalongkorn University Theses and Dissertations (Chula ETD)

Red tilapia (Oreochromis sp.) is a prominent freshwater fish in Thai aquaculture. It has long been afflicted by severe bacterial diseases, ulcerative syndrome, and hemorrhagic septicemia, primarily caused by Aeromonas veronii. Vaccination is a key strategy for control and prevention. In this study a chitosan nano-immersion vaccine has been developed using a nanoemulsion technique and surface modification method with chitosan polymers. The physicochemical properties, mucoadhesive properties, immune response assays, and vaccine efficacy were investigated. The results showed that the vaccine is spherical-shaped, nanometer-sized, and having monodispersed positive charge. The mucoadhesive characterization, observed by SEM and fluorescence microscope imaging, demonstrated that …


The Expressions Of Mhc Class I And Class Ii In Biopsy Tissues By Immunohistochemistry And The Effect Of Interferon-Gamma (Ifn-Γ) On In Vitro Established Canine Cutaneous Mast Cell Tumors, Sudchaya Bhanpattanakul Jan 2023

The Expressions Of Mhc Class I And Class Ii In Biopsy Tissues By Immunohistochemistry And The Effect Of Interferon-Gamma (Ifn-Γ) On In Vitro Established Canine Cutaneous Mast Cell Tumors, Sudchaya Bhanpattanakul

Chulalongkorn University Theses and Dissertations (Chula ETD)

Canine mast cell tumors (MCT), common skin tumors in dogs, present difficulties in treatment, emphasizing the need for effective therapeutic protocols. The cancer-immunity cycle's complex interactions between cancer and the immune system play a crucial role in tumor growth. Tumors often evade immune response by downregulating MHC expression, hindering T-cell recognition. Cancer immunotherapy is a promising approach that utilizes the patient's immune system for combating cancer. Interferon-gamma (IFN-γ), a potent immune substance, plays a crucial role in enhancing MHC expression and immune functionality, crucial for T-cell recognition and cancer cell elimination. Limited research exists on MHC expression, tumor immune response, …


Upscale Production Of Canine Adipose-Derived Mesenchymal Stem Cell-Derived Exosomes For Wound Healing In Vitro, Yudith Violetta Pamulang Jan 2023

Upscale Production Of Canine Adipose-Derived Mesenchymal Stem Cell-Derived Exosomes For Wound Healing In Vitro, Yudith Violetta Pamulang

Chulalongkorn University Theses and Dissertations (Chula ETD)

Mesenchymal stem cell-derived exosomes (MSC-derived exosomes) have been previously reported to enhance wound healing across different phases. However, its practical application has been hindered due to challenges regarding low productivity and the lack of efficient production protocol. Additionally, the usage of exosome-based regenerative treatments in the veterinary field remains relatively unexplored. Hence, this study aimed to address these gaps by establishing a potential upscaling protocol producing canine adipose-derived mesenchymal stem cell-derived exosomes (cAD-MSC-exosomes) and exploring their wound healing potential in vitro. To facilitate production protocol establishment, an in-house exosome-collecting media, designated as VSCBIC-3, was preliminarily developed. Building upon this foundation, …


Contamination Of Antimicrobial-Resistant Escherichia Coli, Salmonella Spp., Enterococcus Faecalis, And Enterococcus Faecium In Commercial Raw Meat-Based Diets For Cats And Dogs, Rusmin Indra Jan 2023

Contamination Of Antimicrobial-Resistant Escherichia Coli, Salmonella Spp., Enterococcus Faecalis, And Enterococcus Faecium In Commercial Raw Meat-Based Diets For Cats And Dogs, Rusmin Indra

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study aimed to investigate the contamination of third-generation cephalosporin (3GC)-resistant Escherichia coli, Salmonella spp., Enterococcus faecalis, and E. faecium in commercial raw meat-based diets (RMBDs) for cats and dogs in Thailand. Fifty RMBD products from ten brands were included for total plate count and bacterial isolation. Antimicrobial susceptibility testing was conducted for the targeted bacteria. Extended-spectrum β-lactamase (ESBL) production and the presence of blaCTX-M and mcr genes were detected. Additionally, E. coli phylogroups and Salmonella serovars were identified. The RMBD samples were contaminated by 3GC-resistant E. coli (80.0%), Salmonella spp. (70.0%), E. faecalis (100%), and E. faecium (84.0%). All …


Volatile Organic Compound Profiles Identifiled In Vaginal Discharge Of Bitches During Estrous Cycle, Amornthep Preechatangkit Jan 2023

Volatile Organic Compound Profiles Identifiled In Vaginal Discharge Of Bitches During Estrous Cycle, Amornthep Preechatangkit

Chulalongkorn University Theses and Dissertations (Chula ETD)

Volatile organic compounds (VOCs) are the metabolites of microorganisms. VOCs are low molecular weight and easily evaporate at room temperature, all odorants are VOC. VOCs are different depending on health status and reproductive status. The objective of this study was to profile the VOCs in bitches during estrous cycle, and to compare VOCs between stages of estrous cycle and between pre-ovulatory, ovulation and post-ovulatory periods. A total of 23 normal, healthy bitches requested for ovulation timing as part of the routine breeding management were used in this study. All animals had a previous history of normal estrous cycle without any …


Reproductive Performance Of Gilts And Sows In Gestation Group Housing And Free Farrowing System In Tropical Climate, Natchanon Dumniem Jan 2023

Reproductive Performance Of Gilts And Sows In Gestation Group Housing And Free Farrowing System In Tropical Climate, Natchanon Dumniem

Chulalongkorn University Theses and Dissertations (Chula ETD)

Animal welfare is a rising topic in the swine industry, resulting in the adoption of alternative housing systems. However, the knowledge of management practices to mitigate the deleterious effects of these systems on reproductive performance in tropical environments is limited. This thesis aims to determine the impacts of alternative housing systems on the reproductive performance of sows and gilts, comprising 4 parts. i) To compare reproductive performance among conventional crates (CC), group housing gestation pen with temporary farrowing crate (PC), and group housing gestation pen and free-farrowing pen (PP). On average, the farrowing rate of sows in the CC group …


Analytical Performance Of Progesterone Testing Using Surface Plasmon Field-Enhanced Fluorescence Spectroscopy Technology In Dogs, Pimuk Sungprasit Jan 2023

Analytical Performance Of Progesterone Testing Using Surface Plasmon Field-Enhanced Fluorescence Spectroscopy Technology In Dogs, Pimuk Sungprasit

Chulalongkorn University Theses and Dissertations (Chula ETD)

A new clinical chemistry analyzer using surface plasmon field-enhanced fluorescence spectroscopy (SPFS) has been introduced into veterinary diagnostic laboratories as a point-of-care unit, enabling rapid turnaround times for both diagnosis and follow-up. Consequently, the test results must be accurate, precise, and reliable. This research aims to evaluate the method performance of the analyzer. The evaluation was conducted according to Clinical Laboratory Improvement Amendments (CLIA) guidelines using method validation. The performance evaluation included studies on imprecision (within-day and between-day), method comparison, recovery tests, and linearity for determining progesterone concentration. The method validation results showed good and acceptable precision. Serum spiking concentrations …


Genetic Diversity Of Influenza A Viruses In Live Bird Markets And Free-Grazing Ducks In Thailand, Supanat Boonyapisitsopa Jan 2023

Genetic Diversity Of Influenza A Viruses In Live Bird Markets And Free-Grazing Ducks In Thailand, Supanat Boonyapisitsopa

Chulalongkorn University Theses and Dissertations (Chula ETD)

Influenza A viruses (IAVs) can infect birds and a wide variety of mammals. Live bird markets (LBMs) and free-grazing ducks (FGDs) are the important reservoirs of influenza A viruses in Thailand. This study aims to gain the overall information of IAVs circulated in LBMs in Bangkok and in FGD flocks in Thailand. IAVs surveillance in LBM in Bangkok was conducted to identified the IAVs in LBM during 2019-2020. The IAV-H9N2 (n=8) and IAV-H9Nx (n=2) were isolated from 1,178 swab samples. The IAVs of the previous CU-EIDAs’ LBM surveillance programs during 2014-2018 were included for overall genetic analysis. Total 6 subtypes …


Molecular Investigation And Genetic Characterization Of Canine Astrovirus And Canine Kobuvirus With Associated Pathology In Domestic Dogs In Vietnam And Thailand, Tin Van Nguyen Jan 2023

Molecular Investigation And Genetic Characterization Of Canine Astrovirus And Canine Kobuvirus With Associated Pathology In Domestic Dogs In Vietnam And Thailand, Tin Van Nguyen

Chulalongkorn University Theses and Dissertations (Chula ETD)

Canine astrovirus (CaAstV) and canine kobuvirus (CaKoV) are recently recognized pathogens associated with canine gastrointestinal disease (GID), though their exact role remains poorly understood. In both Vietnam and Thailand, these viruses were detected in dogs with and without diarrhea, particularly high frequency in puppies under 6 months old. Co-infection between CaAstV and CaKoV, as well as with other common canine enteric viruses (canine parvovirus, canine enteric coronavirus, canine distemper virus), were also confirmed. Complete coding sequences (CDS) of 7 CaAstV strains and 9 CaKoV strains from Vietnam, and 2 CaAstV strains and 4 CaKoV strains from Thailand were successfully identified. …


Innovation Of Alpha-Mangostin Encapsulated With Nanostructured-Lipid Carrier As Non-Surgical Castration In Cats And Survey Attitude Towards Surgical Sterilization In Thailand, Shanaporn Leelakajornkit Jan 2023

Innovation Of Alpha-Mangostin Encapsulated With Nanostructured-Lipid Carrier As Non-Surgical Castration In Cats And Survey Attitude Towards Surgical Sterilization In Thailand, Shanaporn Leelakajornkit

Chulalongkorn University Theses and Dissertations (Chula ETD)

Overpopulation of companion animals, particularly dogs and cats, is a global concern, necessitating effective population control policies. This research investigates the attitudes of veterinarians and pet owners in Thailand regarding dog and cat surgical sterilization and the use of progestins as non-surgical contraception. The study also assesses the effects of intratesticular administration of alpha-mangostin encapsulated in nanostructured lipid carriers (AM-NLC) in male cats, and adverse effects. Furthermore, a novel formulation of AM-NLC for intratesticular administration is also developed. Surveys reveal that decisions on sterilization are influenced by species, sex, COVID-19 impacts, and demographic factors. And anesthesia risks remain a primary …


Developmental Model Of Endometrial Cell Culture For The Study Of Equine Endometrosis, Sawita Santiviparat Jan 2023

Developmental Model Of Endometrial Cell Culture For The Study Of Equine Endometrosis, Sawita Santiviparat

Chulalongkorn University Theses and Dissertations (Chula ETD)

The importance and rationale of this thesis were to address the critical knowledge gap in equine endometrosis by developing a comprehensive 3-dimensional (3D) in vitro model of equine endometrial tissue using advanced technology like 3D bioprinting. Study 1 aimed to develop an in vitro model for studying equine endometrial function by isolating and characterizing equine endometrial epithelial cells (eECs) and endometrial mesenchymal stem cells (eMSCs) using a modified tissue explantation outgrowth technique. Both cell types were successfully cultured in specific medium conditions, maintaining their characteristic features. Notably, eECs retained their function in fetal bovine serum (FBS) and KnockOut™ serum replacement …


A Comprehensive Study Of African Horse Sickness Outbreak In The Northeastern Region Of Thailand: Outbreak Investigation, Genetic Characterization, And Longitudinal Serological Monitoring, Napawan Bunpapong Jan 2023

A Comprehensive Study Of African Horse Sickness Outbreak In The Northeastern Region Of Thailand: Outbreak Investigation, Genetic Characterization, And Longitudinal Serological Monitoring, Napawan Bunpapong

Chulalongkorn University Theses and Dissertations (Chula ETD)

African horse sickness (AHS) results from infection with the African horse sickness virus (AHSV). The AHSV is a non-contagious and belongs to genus Orbivirus, family Reoviridae and primarily by Culicoides midges. AHS caused the devastating infection in equine population, including horses, ponies, European donkeys, and mules. AHS maintains its occurrence in southern African countries, it may possess threatening impact to the naïve regions such as Asia or Southeast Asia. The objectives of this study are 1) To investigate the emerging AHS outbreak in the Northeastern region of Thailand, 2) To characterize emerged AHSV in Thailand and 3) To monitor serological …


Diversity Of Blood-Sucking Insects And Vector-Borne Diseases In Pig Farms In Thailand, Kritsada Thongmeesee Jan 2023

Diversity Of Blood-Sucking Insects And Vector-Borne Diseases In Pig Farms In Thailand, Kritsada Thongmeesee

Chulalongkorn University Theses and Dissertations (Chula ETD)

Vector-borne diseases (VBDs) have been recognized as important diseases in humans and animals across the globe. Blood-sucking insects, such as mosquitoes, tabanid flies, and stable flies may carry pathogens, transmit, and cause VBDs in hosts, including pigs. In Thailand, most of the pig farms have backyard system implying that pigs could be easily exposed to blood-sucking insects and lead to infection with vector-borne pathogens (hemoplasmas, Japanese encephalitis virus (JEV), and Trypanosoma (T.) evansi). However, the information about vector-borne pathogens in pigs in Thailand needs to be updated. The objectives of this dissertation were to study the occurrence and genetic diversity …